Production of bio-fertilizer from Ascophyllum nodosum and Sargassum muticum (Phaeophyceae)
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摘要
Oceans and seas form a large body of water that contains a natural biodiversity. For humans,represents a resource, which makes this a point of interest, from researches to improve the economy. Seaweeds produce many compounds and secondary metabolites that can be used in different fields of industry such as food, agricultural, pharmaceutical and health. Even though seaweeds are ancestral resources, recently it was notorious a global interest in knowing more about its potentials, where biotechnology plays an important role in research. Studies showed that seaweed has many bioactive compounds beneficial to plant development, giving them a great potential as an agricultural fertilizer. Adding seaweeds to the soil provides organic matter, minerals, trace elements, growth plant regulator, metabolites, vitamins, and amino acids and it can work as a soil conditioner. In Portugal, the use of seaweeds for agriculture is important since long time ago. In the past, populations that lived near coastal zone depended on the seaweeds as a family subsistence but, throughout the years, synthetic fertilizers replaced seaweeds. Our work aimed to assess the potential of the extracts obtained from Ascophyllum nodosum and from Sargassum muticum as an agricultural fertilizer.This evaluation was carried out with rice plants(Oryza sativa) and lettuce(Lactuca sativa), in germination bioassays, the culture of rice and lettuce plants in pots, and culture of lettuce plants in hydroponics. For that, seaweed liquid extracts were used in different concentrations in different bioassays. Results show that extracts obtained from two seaweeds, A. nodosum and S. muticum, can be promissory plant biofertilizer at a concentration of 25% and had a positive effect on seed germination, plant development, and production.
Oceans and seas form a large body of water that contains a natural biodiversity. For humans,represents a resource, which makes this a point of interest, from researches to improve the economy. Seaweeds produce many compounds and secondary metabolites that can be used in different fields of industry such as food, agricultural, pharmaceutical and health. Even though seaweeds are ancestral resources, recently it was notorious a global interest in knowing more about its potentials, where biotechnology plays an important role in research. Studies showed that seaweed has many bioactive compounds beneficial to plant development, giving them a great potential as an agricultural fertilizer. Adding seaweeds to the soil provides organic matter, minerals, trace elements, growth plant regulator, metabolites, vitamins, and amino acids and it can work as a soil conditioner. In Portugal, the use of seaweeds for agriculture is important since long time ago. In the past, populations that lived near coastal zone depended on the seaweeds as a family subsistence but, throughout the years, synthetic fertilizers replaced seaweeds. Our work aimed to assess the potential of the extracts obtained from Ascophyllum nodosum and from Sargassum muticum as an agricultural fertilizer.This evaluation was carried out with rice plants(Oryza sativa) and lettuce(Lactuca sativa), in germination bioassays, the culture of rice and lettuce plants in pots, and culture of lettuce plants in hydroponics. For that, seaweed liquid extracts were used in different concentrations in different bioassays. Results show that extracts obtained from two seaweeds, A. nodosum and S. muticum, can be promissory plant biofertilizer at a concentration of 25% and had a positive effect on seed germination, plant development, and production.
Oceans and seas form a large body of water that contains a natural biodiversity. For humans,represents a resource, which makes this a point of interest, from researches to improve the economy. Seaweeds produce many compounds and secondary metabolites that can be used in different fields of industry such as food, agricultural, pharmaceutical and health. Even though seaweeds are ancestral resources, recently it was notorious a global interest in knowing more about its potentials, where biotechnology plays an important role in research. Studies showed that seaweed has many bioactive compounds beneficial to plant development, giving them a great potential as an agricultural fertilizer. Adding seaweeds to the soil provides organic matter, minerals, trace elements, growth plant regulator, metabolites, vitamins, and amino acids and it can work as a soil conditioner. In Portugal, the use of seaweeds for agriculture is important since long time ago. In the past, populations that lived near coastal zone depended on the seaweeds as a family subsistence but, throughout the years, synthetic fertilizers replaced seaweeds. Our work aimed to assess the potential of the extracts obtained from Ascophyllum nodosum and from Sargassum muticum as an agricultural fertilizer.This evaluation was carried out with rice plants(Oryza sativa) and lettuce(Lactuca sativa), in germination bioassays, the culture of rice and lettuce plants in pots, and culture of lettuce plants in hydroponics. For that, seaweed liquid extracts were used in different concentrations in different bioassays. Results show that extracts obtained from two seaweeds, A. nodosum and S. muticum, can be promissory plant biofertilizer at a concentration of 25% and had a positive effect on seed germination, plant development, and production.
引文
Akila N,Jeyadoss T. 2010. The potential of seaweed liquid fertilizer on the growth and antioxidant enhancement of Helianthus annuus L. Oriental Journal of Chemistry,26(4):1 353-1 360.
Anisimov M M, Chaikina E L. 2014. Effect of seaweed extracts on the growth of seedling roots of soybean(Glycine max(L.)Merr.)seasonal changes in the activity. Int. J. Curr.Res. Acad. Rev., 2(3):19-23.
Arioli T, Mattner S W, Winberg P C. 2015. Applications of seaweed extracts in Australian agriculture:past, present and future. Journal of Applied Phycology, 27(5):2 007-2015.
Briceno-Dominguez D, Hernandez-Carmona G, Moyo M,Stirk W, van Staden J. 2014. Plant growth promotingactivity of seaweed liquid extracts produced from Macrocystis pyrifera under different pH and temperature conditions. Journal of Applied Phycology, 26(5):2 203-2210.
Bruno R L A, Viana J S, da Silva V F, Bruno G B, de Moura M F. 2007. Producao e qualidade de sementes e raizes de cenoura cultivada em solo com adubacao organica e mineral. Horticultura Brasileira, 25(2):170-174.
Calvo P, Nelson L, Kloepper J W. 2014. Agricultural uses of plant biostimulants. Plant and Soil, 383(1-2):3-41,https://doi.org/10.1007/s11104-014-2131-8.
Coimbra C S. 2006. Inferencias filogeneticas na ordem Fucales(Phaeophyceae), com enfase no genero Sargassum C.Agardh do Atlantico Sul. Instituto de Biociencias, Sao Paulo, https://doi.org/10.11606/T.41.2006.tde-22082007-101454.
Craigie J S. 2011. Seaweed extract stimuli in plant science and agriculture. Journal of Applied Phycology, 23(3):371-393. https://doi.org/10.1007/s10811-010-9560-4.
Cunha-Santino M B, Gouvea S P, Bianchini Jr I, Vieira A A H.2008. Oxygen uptake during mineralization of photosynthesized carbon from phytoplankton of the Barra Bonita Reservoir:a mesocosm study. Journal of Biology,68(1):115-122.
Dantas N P, Pinheiro J F, Santos J H. 1998. Effects of varying concentrations of Sargassum vulgare C. Agardh on growth of lettuce and coriander. Archives of Marine Sciences, 31:41-46.
Erreira A G. 2004. Interferencia:competicao e alelopatia. In:Ferreira A G, Borghetti F eds. Germinacao:do Basico ao Aplicado. Artmed, Porto Alegre. p.251-262.
Erulan V, Soundarapandian P, Thirumaran G, Ananthan G.2009. Studies on the effect of Sargassum polycystum(C.Agardh, 1824)extract on the growth and biochemical composition of Cajanus cajan(L.)Mill sp. AmericanEurasian J. Agric.&Environ. Sci., 6(4):392-399.
Eyras M C, Defosse G E, Dellatorre F. 2008. Seaweed compost as an amendment for horticultural soils in Patagonia,Argentina. Compost Science&Utilization, 16(2):119-124.
Fernandes A L T, de Oliveira Silva R. 2011. Avaliagao do extrato de algas(Ascophyllum nodosum)no desenvolvimento vegetativo e produtivo do cafeeiro irrigado por gotejamento e cultivado em condicoes de cerrado. Enciclopedia Biosfera, Centro Cientifico Conhecer-Goiania, 7(13):147-157.
Gandhiappan K, Perumal P. 2001. Growth promoting effect of seaweed liquid fertilizer(Enteromorpha intestinalis)on the sesame crop plant. Seaweed Research and Utilization,23(1-2):23-25.
Gressler V. 2010. Composicao quimica e potencial biologico das algas vermelhas marinhas Laurencia filiformis,Laurencia intricata, Plocamium brasiliense e Ochtodes secundiramea da costa brasileira. Faculdade de Ciencias Farmaceuticas, Sao Paulo. https://doi.org/10.11606/T.9.2010.tde-15072011-110848.
Hernandez-Herrera R M, Santacruz-Ruvalcaba F, Ruiz-LopezM A, Norrie J, Hemandez-Carmona G. 2014. Effect of liquid seaweed extracts on growth of tomato seedlings(Solanum lycopersicum L.). Journal of Applied Phycology,26(1):619-628.
Kawakita, E T, de Souza E A, Uehara D M, de Oliveira Orsi R.2015. Shelf-life evaluation of hydroalcoholic propolis extract kept under different storage temperatures. Atas de Saude Ambiental, 3(1):33-46.
Limberger P A, Gheller J A. 2012. Efeito da aplicacao foliar de extrato de algas, aminoácidos e nutrientes via foliar na produtividade e qualidade de alface crespa. Revista Brasileira de Energias Renovaveis, 1:148-161.
Maguire J D. 1962. Speed of germination:aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2(2):176-177.
Mayara Tania P, Deisiane Del Castilo B, Alex Bruno Lobato R,Ryan da Silva R, Sheylla Susan Moreira da Silva de A.2015. Antioxidant and cytotoxic potential of aqueous crude extract of Acmella oleracea(L.)R.K. Jansen.Journal of Chemical and Pharmaceutical Research,7(12):562-569.
McHugh D J. 2003. A guide to the seaweed industry. FAO Fisheries Technical Paper, 441:73-90.
Michalak I, Chojnacka K. 2015. Algae as production systems of bioactive compounds. Engineering in Life Sciences,152(2):160-176.
Monteiro C, Engelen A H, Serrao E A, Santos R. 2009. Habitat differences in the timing of reproduction of the invasive alga Sargassum muticum(Phaeophyta, Sargassaceae)over tidal and lunar cycles. Journal of Phycology, 45(1):1-7.
Moreira G C, Haber L L, Tonin F B, Goto R, Valente MC.2006. Efeito de diferentes 6pocas de aplicacao da alga marinha Ascophyllum nodosum no desenvolvimento da alface. XLVI Congresso Brasileiro de Olericultura. 30/07a 04/08/2006, Goiania, Brasil.
Nabti E, Jha B, Hartmann A. 2017. Impact of seaweeds on agricultural crop production as biofertilizer. International Journal of Environmental Science and Technology, 14(5):1 119-1 134.
Pereira L, Correia F. 2015. Algas Marinhas da Costa Portuguesa-Ecologia, Biodiversidade e Utilizacoes. Nota de Rodap6Edicoes, Paris. 340p.
Pereira L. 2010. Littoral of Viana do Castelo—ALGAE. Uses in agriculture, gastronomy and food industry(Bilingual).Camara Municipal de Viana do Castelo, Viana do Castelo.68p.
Pereira L. 2018a. Ascophyllum nodosum, MACOI-Portuguese Seaweeds Website, MARE, University of Coimbra,Portugal. http://macoi.ci.uc.pt/spec_list_detail.php?spec_id=6Accessed March26, Accessed on 2018.
Pereira L. 2018b. Sargassum muticum. MACOI-Portuguese Seaweeds Website, MARE, University of Coimbra,Portugal. http://macoi.ci.uc.pt/spec_list_detail.php?spec_id=157AccessedMarch26, Accessed on 2018.
Plantytec. 2014. Plantytec Group. http://www.plantytecgroup.com.br/Accessed March26, Accessed on 2018.
Safinaz A F, Ragaa A H 2013. Effect of some red marine algae as biofertilizers on growth of maize(Zea mayz L.)plants.International Food Research Journal, 20(4):1 629-1 632.
Sanchez I, Fernandez C. 2005. Impact of the invasive seaweed Sargassum muticum(Phaeophyta)on an intertidal macroalgal assemblage. Journal of Phycology, 41(5):923-930.
Shekhar Sharma H S, Fleming C, Selby C, Rao J R, Martin T.2014. Plant biostimulants:a review on the processing of macroalgae and use of extracts for crop management to reduce abiotic and biotic stresses. Journal of Applied Phycology, 26(1):465-490.
Silva P M D. 2009. Atividades biologicas de extratos de algas marinhas brasileiras. Dissertacao de mestrado em Bioquimica. Instituto de Quimica, SP. https://doi.org/10.11606/D.46.2010.tde-16062010-084634.
Thirumaran G, Arumugam M, Arumugam R, Anantharaman P.2009. Effect of seaweed liquid fertilizer on growth and pigment concentration of Abelmoschus esculentus(I)Medikus. American-Eurasian Journal of Agronomy, 2(2):57-66.
Veeragurunathan V, Meenakshi Sundaram V, Balachandar C.2011, Comparative studies on fertilizing efficiency of LSF from three seaweeds on the growth of Capsicum annum.Seaweed Res. Utiln.,33(1-2):143-149.
Venkataraman Kumar V, Mohan R, Murugeswari R,Muthusamy M. 1993. Effect of crude and commercial seaweed extracts on seed germination and seedling growth in green gram and black gram. Seaweed Res Utiln.,16(1&2):23-27.
Vijayanand N, Ramya S S, Rathinavel S. 2014. Potential of liquid extracts of Sargassum wightii on growth,biochemical and yield parameters of cluster bean plant.Asian Pacific Journal of Reproduction, 3(2):150-155.
Wang J, Liu S, Qin Y K, Chen X L, Xiong R E, Yu H H, Li K C, Li P C. 2017. Preparation and characterization of controlled-release fertilizers coated with marine polysaccharide derivatives. Chinese Journal of Oceanology and Limnology, 35(5):1 086-1 093.
Zheng S Y, He M L, Jiang J, Zou S M, Yang W N, Zhang Y,Deng J, Wang C H. 2016. Effect of kelp waste extracts on the growth and lipid accumulation of microalgae.Bioresource Technology, 201:80-88.
Anisimov M M, Chaikina E L. 2014. Effect of seaweed extracts on the growth of seedling roots of soybean(Glycine max(L.)Merr.)seasonal changes in the activity. Int. J. Curr.Res. Acad. Rev., 2(3):19-23.
Arioli T, Mattner S W, Winberg P C. 2015. Applications of seaweed extracts in Australian agriculture:past, present and future. Journal of Applied Phycology, 27(5):2 007-2015.
Briceno-Dominguez D, Hernandez-Carmona G, Moyo M,Stirk W, van Staden J. 2014. Plant growth promotingactivity of seaweed liquid extracts produced from Macrocystis pyrifera under different pH and temperature conditions. Journal of Applied Phycology, 26(5):2 203-2210.
Bruno R L A, Viana J S, da Silva V F, Bruno G B, de Moura M F. 2007. Producao e qualidade de sementes e raizes de cenoura cultivada em solo com adubacao organica e mineral. Horticultura Brasileira, 25(2):170-174.
Calvo P, Nelson L, Kloepper J W. 2014. Agricultural uses of plant biostimulants. Plant and Soil, 383(1-2):3-41,https://doi.org/10.1007/s11104-014-2131-8.
Coimbra C S. 2006. Inferencias filogeneticas na ordem Fucales(Phaeophyceae), com enfase no genero Sargassum C.Agardh do Atlantico Sul. Instituto de Biociencias, Sao Paulo, https://doi.org/10.11606/T.41.2006.tde-22082007-101454.
Craigie J S. 2011. Seaweed extract stimuli in plant science and agriculture. Journal of Applied Phycology, 23(3):371-393. https://doi.org/10.1007/s10811-010-9560-4.
Cunha-Santino M B, Gouvea S P, Bianchini Jr I, Vieira A A H.2008. Oxygen uptake during mineralization of photosynthesized carbon from phytoplankton of the Barra Bonita Reservoir:a mesocosm study. Journal of Biology,68(1):115-122.
Dantas N P, Pinheiro J F, Santos J H. 1998. Effects of varying concentrations of Sargassum vulgare C. Agardh on growth of lettuce and coriander. Archives of Marine Sciences, 31:41-46.
Erreira A G. 2004. Interferencia:competicao e alelopatia. In:Ferreira A G, Borghetti F eds. Germinacao:do Basico ao Aplicado. Artmed, Porto Alegre. p.251-262.
Erulan V, Soundarapandian P, Thirumaran G, Ananthan G.2009. Studies on the effect of Sargassum polycystum(C.Agardh, 1824)extract on the growth and biochemical composition of Cajanus cajan(L.)Mill sp. AmericanEurasian J. Agric.&Environ. Sci., 6(4):392-399.
Eyras M C, Defosse G E, Dellatorre F. 2008. Seaweed compost as an amendment for horticultural soils in Patagonia,Argentina. Compost Science&Utilization, 16(2):119-124.
Fernandes A L T, de Oliveira Silva R. 2011. Avaliagao do extrato de algas(Ascophyllum nodosum)no desenvolvimento vegetativo e produtivo do cafeeiro irrigado por gotejamento e cultivado em condicoes de cerrado. Enciclopedia Biosfera, Centro Cientifico Conhecer-Goiania, 7(13):147-157.
Gandhiappan K, Perumal P. 2001. Growth promoting effect of seaweed liquid fertilizer(Enteromorpha intestinalis)on the sesame crop plant. Seaweed Research and Utilization,23(1-2):23-25.
Gressler V. 2010. Composicao quimica e potencial biologico das algas vermelhas marinhas Laurencia filiformis,Laurencia intricata, Plocamium brasiliense e Ochtodes secundiramea da costa brasileira. Faculdade de Ciencias Farmaceuticas, Sao Paulo. https://doi.org/10.11606/T.9.2010.tde-15072011-110848.
Hernandez-Herrera R M, Santacruz-Ruvalcaba F, Ruiz-LopezM A, Norrie J, Hemandez-Carmona G. 2014. Effect of liquid seaweed extracts on growth of tomato seedlings(Solanum lycopersicum L.). Journal of Applied Phycology,26(1):619-628.
Kawakita, E T, de Souza E A, Uehara D M, de Oliveira Orsi R.2015. Shelf-life evaluation of hydroalcoholic propolis extract kept under different storage temperatures. Atas de Saude Ambiental, 3(1):33-46.
Limberger P A, Gheller J A. 2012. Efeito da aplicacao foliar de extrato de algas, aminoácidos e nutrientes via foliar na produtividade e qualidade de alface crespa. Revista Brasileira de Energias Renovaveis, 1:148-161.
Maguire J D. 1962. Speed of germination:aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2(2):176-177.
Mayara Tania P, Deisiane Del Castilo B, Alex Bruno Lobato R,Ryan da Silva R, Sheylla Susan Moreira da Silva de A.2015. Antioxidant and cytotoxic potential of aqueous crude extract of Acmella oleracea(L.)R.K. Jansen.Journal of Chemical and Pharmaceutical Research,7(12):562-569.
McHugh D J. 2003. A guide to the seaweed industry. FAO Fisheries Technical Paper, 441:73-90.
Michalak I, Chojnacka K. 2015. Algae as production systems of bioactive compounds. Engineering in Life Sciences,152(2):160-176.
Monteiro C, Engelen A H, Serrao E A, Santos R. 2009. Habitat differences in the timing of reproduction of the invasive alga Sargassum muticum(Phaeophyta, Sargassaceae)over tidal and lunar cycles. Journal of Phycology, 45(1):1-7.
Moreira G C, Haber L L, Tonin F B, Goto R, Valente MC.2006. Efeito de diferentes 6pocas de aplicacao da alga marinha Ascophyllum nodosum no desenvolvimento da alface. XLVI Congresso Brasileiro de Olericultura. 30/07a 04/08/2006, Goiania, Brasil.
Nabti E, Jha B, Hartmann A. 2017. Impact of seaweeds on agricultural crop production as biofertilizer. International Journal of Environmental Science and Technology, 14(5):1 119-1 134.
Pereira L, Correia F. 2015. Algas Marinhas da Costa Portuguesa-Ecologia, Biodiversidade e Utilizacoes. Nota de Rodap6Edicoes, Paris. 340p.
Pereira L. 2010. Littoral of Viana do Castelo—ALGAE. Uses in agriculture, gastronomy and food industry(Bilingual).Camara Municipal de Viana do Castelo, Viana do Castelo.68p.
Pereira L. 2018a. Ascophyllum nodosum, MACOI-Portuguese Seaweeds Website, MARE, University of Coimbra,Portugal. http://macoi.ci.uc.pt/spec_list_detail.php?spec_id=6Accessed March26, Accessed on 2018.
Pereira L. 2018b. Sargassum muticum. MACOI-Portuguese Seaweeds Website, MARE, University of Coimbra,Portugal. http://macoi.ci.uc.pt/spec_list_detail.php?spec_id=157AccessedMarch26, Accessed on 2018.
Plantytec. 2014. Plantytec Group. http://www.plantytecgroup.com.br/Accessed March26, Accessed on 2018.
Safinaz A F, Ragaa A H 2013. Effect of some red marine algae as biofertilizers on growth of maize(Zea mayz L.)plants.International Food Research Journal, 20(4):1 629-1 632.
Sanchez I, Fernandez C. 2005. Impact of the invasive seaweed Sargassum muticum(Phaeophyta)on an intertidal macroalgal assemblage. Journal of Phycology, 41(5):923-930.
Shekhar Sharma H S, Fleming C, Selby C, Rao J R, Martin T.2014. Plant biostimulants:a review on the processing of macroalgae and use of extracts for crop management to reduce abiotic and biotic stresses. Journal of Applied Phycology, 26(1):465-490.
Silva P M D. 2009. Atividades biologicas de extratos de algas marinhas brasileiras. Dissertacao de mestrado em Bioquimica. Instituto de Quimica, SP. https://doi.org/10.11606/D.46.2010.tde-16062010-084634.
Thirumaran G, Arumugam M, Arumugam R, Anantharaman P.2009. Effect of seaweed liquid fertilizer on growth and pigment concentration of Abelmoschus esculentus(I)Medikus. American-Eurasian Journal of Agronomy, 2(2):57-66.
Veeragurunathan V, Meenakshi Sundaram V, Balachandar C.2011, Comparative studies on fertilizing efficiency of LSF from three seaweeds on the growth of Capsicum annum.Seaweed Res. Utiln.,33(1-2):143-149.
Venkataraman Kumar V, Mohan R, Murugeswari R,Muthusamy M. 1993. Effect of crude and commercial seaweed extracts on seed germination and seedling growth in green gram and black gram. Seaweed Res Utiln.,16(1&2):23-27.
Vijayanand N, Ramya S S, Rathinavel S. 2014. Potential of liquid extracts of Sargassum wightii on growth,biochemical and yield parameters of cluster bean plant.Asian Pacific Journal of Reproduction, 3(2):150-155.
Wang J, Liu S, Qin Y K, Chen X L, Xiong R E, Yu H H, Li K C, Li P C. 2017. Preparation and characterization of controlled-release fertilizers coated with marine polysaccharide derivatives. Chinese Journal of Oceanology and Limnology, 35(5):1 086-1 093.
Zheng S Y, He M L, Jiang J, Zou S M, Yang W N, Zhang Y,Deng J, Wang C H. 2016. Effect of kelp waste extracts on the growth and lipid accumulation of microalgae.Bioresource Technology, 201:80-88.