摘要
目的:检测肝癌组织中富含脯氨酸的酪氨酸蛋白激酶2(proline-rich tyrosine kinase-2,Pyk2)、Twist和基质金属蛋白酶-7(MMP-7)的表达,并分析它们相互之间的关系及其临床意义。方法:应用免疫组织化学法检测80例肝癌组织及癌旁肝组织中Pyk2、Twist及MMP-7蛋白的表达情况,分析其与临床病理因素的关系。结果:Pyk2在肝癌组织及癌旁肝组织中的阳性表达率分别为71.3%、12.0%,差异有统计学意义(P<0.05);Twist在肝癌组织及癌旁肝组织中的阳性表达率分别为66.3%、6.0%,差异有统计学意义(P<0.05);MMP-7在肝癌组织及癌旁肝组织中的阳性表达率分别为68.8%、8.0%,差异有统计学意义(P<0.05)。Pyk2阳性表达与肝癌病理分级、分化程度、血管侵犯及TNM分期明显相关(P<0.05),Twist阳性表达与肝癌病理分级、分化程度、血管侵犯及TNM分期明显相关(P<0.05),MMP-7阳性表达与肝癌病理分级、分化程度、血管侵犯及TNM分期明显相关(P<0.05)。肝癌组织中,Pyk2与Twist表达呈正相关(P<0.05),Pyk2和MMP-7表达亦呈正相关(P<0.05),Twist和MMP-7表达亦呈正相关(P<0.05)。结论:Pyk2、Twist和MMP-7蛋白的表达水平在肝癌组织中与肿瘤组织的分化程度、病理分级、血管侵犯及临床TNM分期等均有关。因此,临床联合检测Pyk2、Twist和MMP-7对肝癌患者的预后判断具有重要意义。
Objective:To study the expressions and their clinical significance of Pyk2,Twist and MMP-7 in hepatocellular carcinoma.Method:The expressions of Pyk2,Twist and MMP-7 proteins were tested by immunohistochemistry in 80 cases of hepatocellular carcinomas and adjacent paracancerous tissues.Result:The positive rate of Pyk2 in hepatocellular carcinomas was significantly higher than that of adjacent paracancerous tissues(71.3% vs 12.0%,P<0.05).Significant differences were also observed in the expression rate of Twist between the patients with hepatocellular carcinomas and paracancerous tissues(66.3% vs 6.0%,P<0.05).The positive rate of MMP-7 in hepatocellular carcinomas was significantly higher than that of adjacent paracancerous tissues(68.8% vs 8.0%,P<0.05).The differences of the expression of Pyk2 in hepatocellular carcinoma of pathological grading,tumor differentiation,vascular invasion and clinical TNM stage were significant(P<0.05).The differences of the expression of Twist in hepatocellular carcinoma of pathological grading,differentiation,vascular invasion and clinical TNM stage were also significant(P<0.05).The differences of the expression of MMP-7 in hepatocellular carcinoma of pathological grading,differentiation,vascular invasion and clinical TNM stage were also significant(P<0.05).Significantly positive correlation was found between Pyk2 and Twist by using spearman correlation analysis(P<0.05).There was positive correlation between Pyk2 and MMP-7(P<0.05).There was positive correlation between Twist and MMP-7(P<0.05).Conclusion:The expressions of Pyk2,Twist and MMP-7 are related to the pathological grading,differentiation,vascular invasion and clinical TNM stage,and their combined detection can help estimate the outcomes of the patients.
引文
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