RhoC基因对胆管癌细胞株QBC939细胞增殖和cyclinD1基因的影响
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摘要
目的研究RhoC基因对胆管癌QBC939细胞株的增殖及cyclinD1 mRNA表达的影响。方法将正、反义RhoC cDNA真核表达载体,转染人胆管癌细胞QBC939,检测细胞生长曲线,RT-PCR检测cyclinD1在QBC939细胞、转染空载体的QBC939细胞(QBC939-V)、转染正义RhoC的QBC939细胞(QBC939-S)、转染反义RhoC的QBC939细胞(QBC939-AS)的mRNA相对表达量。结果与QBC939及QBC939-V对照细胞相比,QBC939-S体外生长较快,QBC939-AS体外生长较慢,cyclinD1表达在QBC939-AS与对照组减少(P<0.05),cyclinD1表达在QBC939-S与对照组增加(P<0.05)。结论 RhoC可能通过调节cyclinD1来调控QBC939的增殖能力。
Objective To observe the effect of RhoC gene on QBC939 cells' proliferation and cyclinD1 gene expression. Methods Sense、Antisence eukaryotic expression plasmid of RhoC were transfected into QBC939 by Lipofectamine method.RT - PCR was used to examine the expression of Hygromycin B and cyclinD1,the changes of proliferation were checked by growth analysis in vitro.Results The difference of cyclinD1 expression in QBC939 - AS group、QBC939 - S group and in control group was statistically significant(P <0.05),Compared with the control QBC939 cells and QBC939 - V cells,QBC939 -AS grew more slowly,but QBC939 -S grew more quickly.Conclusion RhoC could control the proliferation of cholangiocarcinoma cell lines QBC939 cells by control the expression of cyclinD1.
Objective To observe the effect of RhoC gene on QBC939 cells' proliferation and cyclinD1 gene expression. Methods Sense、Antisence eukaryotic expression plasmid of RhoC were transfected into QBC939 by Lipofectamine method.RT - PCR was used to examine the expression of Hygromycin B and cyclinD1,the changes of proliferation were checked by growth analysis in vitro.Results The difference of cyclinD1 expression in QBC939 - AS group、QBC939 - S group and in control group was statistically significant(P <0.05),Compared with the control QBC939 cells and QBC939 - V cells,QBC939 -AS grew more slowly,but QBC939 -S grew more quickly.Conclusion RhoC could control the proliferation of cholangiocarcinoma cell lines QBC939 cells by control the expression of cyclinD1.
引文
[1]A D Ridley.The GTP - binding Protein Rho.J Biochem Cell Biol, 1997,23:1225-1229.
[2]石铮,王家兴,何庆良,等.人RhoC基因正、反义真核表达载体的构建及对肿瘤增殖的影响.中华实验外科杂志,2005,22: 1289-1291.
[3]Chardin P,Madaule P,Tavitian A,et al.Coding sequence of human rho cDNAs clone 6 and clone 9.Nucleic Acid Res,1988,16(6): 2717-2717.
[4]Fagan K.Oliveira L.Pittler S.Sequence of rho small GTP - binding protein cDNAs from human retina and identification of novel 5 - prime end cloning artifacts.Exp Eye Res,1994,59:235 -237.
[5]Anne J.Ridley.Rho proteins and cancer.Breast Cancer Research and Treatment,2004,84:13-19.
[6]石铮,郑岩松,曾金华,等.RhoC基因及蛋白在胃癌肝转移中的表达及意义.中华实验外科杂志,2002,19:310-311.
[7]王亮,金锡御,潘进洪,等.内皮抑制素抑制内皮细胞增殖的细胞周期调控机制.中华实验外科杂志,2003,20:821-822.
[8]蔡圣强,王建江,陈谢周,等.胆管癌细胞cyvclinDl与P16蛋白异常表达.中华新医学,2002,3:867-868.
[9]Rees RW,Foxwell NA,Alph RD,et al.Y - 27632,a Rho - kinase inhibitor, inhibits proliferation and adrenergic contraction of prostatic smooth muscle cells.J Urol,2003,170:2517 -2522.
[10]Welsh CF.Rho GTPases as key transducers of proliferative signals in gl cell cycle regulation.Breast Cancer Research and Treatment, 2004,84:33-42.
[11]Fuse M,Tanalsa T,Shibata T.Regulation of geranylgeranyl pyrophosphate synthase in the proliferation of rat FRTL -5 cells:involvement of both cAMP - PKA and PI3 - AKT pathways.Biochem Biophys Res Commun,2004,315:1147 -1153.
[2]石铮,王家兴,何庆良,等.人RhoC基因正、反义真核表达载体的构建及对肿瘤增殖的影响.中华实验外科杂志,2005,22: 1289-1291.
[3]Chardin P,Madaule P,Tavitian A,et al.Coding sequence of human rho cDNAs clone 6 and clone 9.Nucleic Acid Res,1988,16(6): 2717-2717.
[4]Fagan K.Oliveira L.Pittler S.Sequence of rho small GTP - binding protein cDNAs from human retina and identification of novel 5 - prime end cloning artifacts.Exp Eye Res,1994,59:235 -237.
[5]Anne J.Ridley.Rho proteins and cancer.Breast Cancer Research and Treatment,2004,84:13-19.
[6]石铮,郑岩松,曾金华,等.RhoC基因及蛋白在胃癌肝转移中的表达及意义.中华实验外科杂志,2002,19:310-311.
[7]王亮,金锡御,潘进洪,等.内皮抑制素抑制内皮细胞增殖的细胞周期调控机制.中华实验外科杂志,2003,20:821-822.
[8]蔡圣强,王建江,陈谢周,等.胆管癌细胞cyvclinDl与P16蛋白异常表达.中华新医学,2002,3:867-868.
[9]Rees RW,Foxwell NA,Alph RD,et al.Y - 27632,a Rho - kinase inhibitor, inhibits proliferation and adrenergic contraction of prostatic smooth muscle cells.J Urol,2003,170:2517 -2522.
[10]Welsh CF.Rho GTPases as key transducers of proliferative signals in gl cell cycle regulation.Breast Cancer Research and Treatment, 2004,84:33-42.
[11]Fuse M,Tanalsa T,Shibata T.Regulation of geranylgeranyl pyrophosphate synthase in the proliferation of rat FRTL -5 cells:involvement of both cAMP - PKA and PI3 - AKT pathways.Biochem Biophys Res Commun,2004,315:1147 -1153.