溃结安康汤对溃疡性结肠炎模型大鼠结肠黏膜MEK/ERK信号通路调控作用
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摘要
目的:观察溃结安康汤对TNBS诱导的大鼠溃疡性结肠炎(ulcerative colitis,UC)的治疗作用,并探讨其可能机制。方法:将48只清洁级SD大鼠,体质量(200±20)g,将其随机分为4组,分别为正常组、模型组、溃结安康汤组、SASP组。造模并治疗21d后,进行DAI评分,观察结肠HE染色病理涂片,MEKmRNA、ERKmRNA表达。结果:与模型组相比较,溃结安康汤组DAI评分降低,结肠HE染色病理示炎症细胞明显减少,MEKmRNA、ERKmRNA表达均升高,均具有显著性差异(P<0.05)。结论:溃结安康汤可以显著改善UC大鼠症状、病理形态,通过调高MEKmRNA、ERKmRNA表达,从而达到抑制炎症、改善症状的效果。
Objective:To observe the effects of Kuijie Ankang Formula on rats of ulcerative colitis(UC) and explore its possible mechanism.Methods:Forty-eight SD rats,body weighted(200±20)g were randomly divided into 4 groups:control group,model group,Kuijie Ankang Formula group and SASP group.After 21 days′ treatment,we recordedDAI score,observed colonic HE staining pathological smear, expressions of MEKmRNA and ERKmRNA on colonic mucosa of ulcerative colitis of rats.Results:To compare with the model group,the Kuijie Ankang Formula group′s DAI index showed gradual decreasing trend and pathological morphology was significantly improved.The expressions of MEKmRNA and ERKmRNA were increased and there were significant difference(P<0.05).Conclusion:Kuijie Ankang Formula could significantly improve the symptoms and pathological morphology in UC rats by increasing the expressions of MEKmRNA and ERKmRNA to reach the effect of inhibiting inflammation.
Objective:To observe the effects of Kuijie Ankang Formula on rats of ulcerative colitis(UC) and explore its possible mechanism.Methods:Forty-eight SD rats,body weighted(200±20)g were randomly divided into 4 groups:control group,model group,Kuijie Ankang Formula group and SASP group.After 21 days′ treatment,we recordedDAI score,observed colonic HE staining pathological smear, expressions of MEKmRNA and ERKmRNA on colonic mucosa of ulcerative colitis of rats.Results:To compare with the model group,the Kuijie Ankang Formula group′s DAI index showed gradual decreasing trend and pathological morphology was significantly improved.The expressions of MEKmRNA and ERKmRNA were increased and there were significant difference(P<0.05).Conclusion:Kuijie Ankang Formula could significantly improve the symptoms and pathological morphology in UC rats by increasing the expressions of MEKmRNA and ERKmRNA to reach the effect of inhibiting inflammation.
引文
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[2] 中国中西医结合学会消化系统疾病专业委员会.溃疡性结肠炎中西医结合诊疗指南(草案)[J].中国中西医结合消化杂志,2011,19(1):61-65.
[3] 柳越冬,陶弘武,王长洪.优化溃结方对溃疡性结肠炎大鼠模型结肠组织表皮生长因子影响的实验研究[J].辽宁中医杂志,2012,39(2):240-242.
[4] 柳越冬,陶弘武,王长洪.优化溃结方对溃疡性结肠炎大鼠模型白细胞介素-1β的含量及蛋白表达的影响[C]// 首届世界中医药大会夏季峰会暨“一带一路”中医药发展国际研讨会,2015.
[5] Wang Y H,Ge B,Yang X L,et al.Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitis[J].International Immunopharmacology,2011,11(10):1620-1627.
[6] Li W G,Zhang X Y,Wu Y J,et al.Anti-inflammatory effect and mechanism of proanthocyanidins from grape seeds[J].Acta Pharmacologica Sinica,2001,22(12):1117-1120.
[7] 陈奇.中药药理研究方法学[M].北京:人民卫生出版社,2000:1024.
[8] 王坤,宣秀敏,王莲,等.溃疡性结肠炎大鼠外周血CD4+ CD25+ Treg的变化及相关性研究[J].安徽医科大学学报,2012,47(3):354-356.
[9] 柳越冬,陶弘武,田振国.慢性非特异性溃疡性结肠炎中西医结合治疗进展[J].中华中医药学刊,2009,27(5):1002-1004.
[10] 吴崑,严红丽,穆敬平.MMP-2、MMP-9与溃疡性结肠炎患者肠黏膜通透性的相关性研究[J].临床与病理杂志,2016,36(4):384-388.
[11] Zhou L,Tan X,Kamohara H,et al.MEK1 and MEK2 isoforms regulate distinct functions in pancreatic cancer cells[J].Oncology Reports,2010,24(1):251-255.
[12] Harding A,Giles N,Burgess A,et al.Mechanism of mitosis-specific activation of MEK1[J].Journal of Biological Chemistry,2003,278(19):16747-16754.
[13] 曹燕飞.疏肝健脾法对溃疡性结肠炎大鼠血清IL-6、IL-10水平及结肠组织Mek1/2基因表达影响的研究[D].兰州:甘肃中医学院,2014.
[14] 宋宁.溃结灵对溃疡性结肠炎大鼠结肠黏膜ITF表达及MEK/ERK通路的影响[D].广州:广州中医药大学,2008.