Structural Characterization of Glucan Produced by Lactobacillus sake L-7 from Sausage
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摘要
A linear glucan was produced by Lactobacillus sake L-7 isolated from homemade sausage. Cultivation of the strain in Man–Rogosa–Sharpe(MRS) medium containing 50 g/L sucrose yielded 5.3 g/L of purified exopolysaccharide(EPS). The EPS was characterized by gas chromatography(GC), Fourier-transform infrared(FT-IR) spectroscopy, high-performance sizeexclusion chromatography(HPSEC), nuclear magnetic resonance(NMR) spectroscopy, and scanning electron microscopy(SEM). The monosaccharide composition of the EPS was glucose, and its molecular weight was 1 × 10~7 Da. The FT-IR and NMR spectra revealed that the L-7 EPS was a linear glucan with α-(1 → 6) glucosidic bonds. SEM images of the dried EPS revealed a hollow tubular structure. The water solubility index and water holding capacity of L-7 EPS were 96 and 272%, respectively. The results of hydrolysis indicated that L-7 EPS was not susceptible to hydrolysis by physiological barriers and can be used as a soluble dietary fiber with health benefits. All these characteristics suggest that L-7 EPS might have potential applications in the food, cosmetic, and pharmaceutical industries.
A linear glucan was produced by Lactobacillus sake L-7 isolated from homemade sausage. Cultivation of the strain in Man–Rogosa–Sharpe(MRS) medium containing 50 g/L sucrose yielded 5.3 g/L of purified exopolysaccharide(EPS). The EPS was characterized by gas chromatography(GC), Fourier-transform infrared(FT-IR) spectroscopy, high-performance sizeexclusion chromatography(HPSEC), nuclear magnetic resonance(NMR) spectroscopy, and scanning electron microscopy(SEM). The monosaccharide composition of the EPS was glucose, and its molecular weight was 1 × 10~7 Da. The FT-IR and NMR spectra revealed that the L-7 EPS was a linear glucan with α-(1 → 6) glucosidic bonds. SEM images of the dried EPS revealed a hollow tubular structure. The water solubility index and water holding capacity of L-7 EPS were 96 and 272%, respectively. The results of hydrolysis indicated that L-7 EPS was not susceptible to hydrolysis by physiological barriers and can be used as a soluble dietary fiber with health benefits. All these characteristics suggest that L-7 EPS might have potential applications in the food, cosmetic, and pharmaceutical industries.
A linear glucan was produced by Lactobacillus sake L-7 isolated from homemade sausage. Cultivation of the strain in Man–Rogosa–Sharpe(MRS) medium containing 50 g/L sucrose yielded 5.3 g/L of purified exopolysaccharide(EPS). The EPS was characterized by gas chromatography(GC), Fourier-transform infrared(FT-IR) spectroscopy, high-performance sizeexclusion chromatography(HPSEC), nuclear magnetic resonance(NMR) spectroscopy, and scanning electron microscopy(SEM). The monosaccharide composition of the EPS was glucose, and its molecular weight was 1 × 10~7 Da. The FT-IR and NMR spectra revealed that the L-7 EPS was a linear glucan with α-(1 → 6) glucosidic bonds. SEM images of the dried EPS revealed a hollow tubular structure. The water solubility index and water holding capacity of L-7 EPS were 96 and 272%, respectively. The results of hydrolysis indicated that L-7 EPS was not susceptible to hydrolysis by physiological barriers and can be used as a soluble dietary fiber with health benefits. All these characteristics suggest that L-7 EPS might have potential applications in the food, cosmetic, and pharmaceutical industries.
引文
1.Ahmed Z,Wang YP,Anjum N et al(2013)Characterization of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefi r-Part II.Food Hydrocoll30(1):343-350
2.Sathiyanaryanan G,Dineshkumar K,Yang YH(2017)Microbial exopolysaccharide-mediated synthesis and stabilization of metal nanoparticles.Crit Rev Microbiol 43(6):731-752
3.Cui JD,Zhang BZ(2011)Comparison of culture methods on exopolysaccharide production in the submerged culture of Cordyceps militaris and process optimization.Lett Appl Microbiol 52(2):123-128
4.Ibarburu I,Puertas AI,Berregi I et al(2015)Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.Int J Food Microbiol 214:54-62
5.Bounaix MS,Gabriel V,Robert H et al(2010)Characterization of glucan-producing Leuconostoc strains isolated from sourdough.Int J Food Microbiol 144(1):1-9
6.Du RP,Xing HW,Zhou ZJ et al(2017)Isolation,characterisation,and fermentation optimisation of glucansucrase producing Leuconostoc mesenteroides DRP105 from sauerkraut with improved preservation stability.Int J Food Sci Technol 52(12):2522-2530
7.Cui JD,Qiu JQ(2012)Production of extracellular water insoluble polysaccharide from Pseudomonas sp.J Agric Food Chem60(19):4865-4871
8.Baruah R,Maina NH,Katina K et al(2017)Functional food applications of dextran from Weissella cibaria RBA12 from pummelo(Citrus maxima).Int J Food Microbiol 242:124-131
9.Schmid J(2018)Recent insights in microbial exopolysaccharide biosynthesis and engineering strategies.Curr Opin Biotechnol53:130-136
10.Zarour K,Llamas MG,Prieto A et al(2017)Rheology and bioactivity of high molecular weight dextrans synthesised by lactic acid bacteria.Carbohydr Polym 174:646-657
11.Kanmani P,Satish KR,Yuvaraj N et al(2011)Production and purifi cation of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro.Bioresour Technol 102(7):4827-4833
12.Maina NH,Tenkanen M,Maaheimo H et al(2008)NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa.Carbohydr Res 343(9):1446-1455
13.Leathers TD,Nunnally MS,Cote GL(2009)Modifi cation of alternan by dextranase.Biotechnol Lett 31(2):289-293
14.Yuliani E,Imai T,Teeka J et al(2011)Exopolysaccharide production from sweet potato-shochu distillery wastewater by Lactobacillus sakei CY1.Biotechnol Biotechnol Equip 25(2):2329-2333
15.Robijn GW,Van den Berg DJC,Haas H et al(1995)Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0?1.Carbohydr Res 276(1):117-136
16.Nácher-Vázquez M,Ballesteros N,Canalesáet al(2015)Dextrans produced by lactic acid bacteria exhibit antiviral and immunomodulatory activity against salmonid viruses.Carbohydr Polym124:292-301
17.Yang YP,Peng Q,Guo YY et al(2015)Isolation and characterization of dextran produced by Leuconostoc citreum NM105 from manchurian sauerkraut.Carbohydr Polym 133:365-372
18.Ai LZ,Guo QB,Ding HH et al(2016)Structure characterization of exopolysaccharides from Lactobacillus casei LC2W from skim milk.Food Hydrocoll 56:134-143
19.Trabelsi I,Slima SB,Chaabane H et al(2015)Purifi cation and characterization of a novel exopolysaccharides produced by Lactobacillus sp.Ca6.Int J Biol Macromol 74:541-546
20.Kavitake D,Devi PB,Singh SP et al(2016)Characterization of a novel galactan produced by Weissella confusa KR780676 from an acidic fermented food.Int J Biol Macromol 86:681-689
21.Saravanan C,Shetty PKH(2016)Isolation and characterization of exopolysaccharide from Leuconostoc lactis KC117496 isolated from idli batter.Int J Biol Macromol 90:100-106
22.Van den Berg DJC,Robijn GW,Janssen AC et al(1995)Production of a novel extracellular polysaccharide by Lactobacillus sake 0-1 and characterization of the polysaccharide.Appl Environ Microbiol 61(8):2840-2844
23.Wang J,Zhao X,Tian Z et al(2015)Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11isolated from Tibet Kefi r.Carbohydr Polym 125:16-25
24.Miao M,Bai AJ,Jiang B et al(2014)Characterisation of a novel water-soluble polysaccharide from Leuconostoc citreum SK24.002.Food Hydrocoll 36:265-272
25.Ahmed RZ,Siddiqui K,Arman M et al(2012)Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3.Carbohydr Polym 90(1):441-446
26.Du RP,Xing HW,Yang YP et al(2017)Optimization,purifi cation and structural characterization of a dextran produced by L.mesenteroides isolated from Chinese sauerkraut.Carbohydr Polym174:409-416
27.Bounaix MS,Gabriel V,Morel S et al(2009)Biodiversity of exopolysaccharides produced from sucrose by sourdough lactic acid bacteria.J Agric Food Chem 57(22):10889-10897
28.Freitas F,Alves VD,Reis MAM(2011)Advances in bacterial exopolysaccharides:from production to biotechnological applications.Trends Biotechnol 29(8):388-398
29.Lacaze G,Wick M,Cappelle S(2007)Emerging fermentation technologies:development of novel sourdoughs.Food Microbiol24(2):155-160
30.Tian YT,Zhao YT,Zeng HL et al(2016)Structural characterization of a novel neutral polysaccharide from Lentinus giganteus and its antitumor activity through inducing apoptosis.Carbohydr Polym 154:231-240
31.Sun YX,Liu JC,Yang XD et al(2010)Purifi cation,structural analysis and hydroxyl radical-scavenging capacity of a polysaccharide from the fruiting bodies of Russula virescens.Process Biochem 45(6):874-879
32.He YL,Ye M,Du ZZ et al(2014)Purifi cation,characterization and promoting eff ect on wound healing of an exopolysaccharide from Lachnum YM405.Carbohydr Polym 105:169-176
33.Shao L,Wu ZJ,Zhang H et al(2014)Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.Carbohydr Polym 107:51-56
34.Seymour FR,Knapp RD,Bishop SH(1976)Determination of the structure of dextran by 13 C nuclear magnetic resonance spectroscopy.Carbohydr Res 51(2):179-194
35.Uzochukwu S,Balogh E,Loefl er RT et al(2001)Structural analysis by 13 C-nuclear magnetic resonance spectroscopy of glucans elaborated by gum-producing bacteria isolated from palm wine.Food Chem 73(2):225-233
36.Purama RK,Goswami P,Khan AT et al(2009)Structural analysis and properties of dextran produced by Leuconostoc mesenteroides NRRL B-640.Carbohydr Polym 76(1):30-35
37.Li CC,Li W,Chen XH et al(2014)Microbiological,physicochemical and rheological properties of fermented soymilk produced with exopolysaccharide(EPS)producing lactic acid bacteria strains.LWT Food Sci Technol 57(2):477-485
2.Sathiyanaryanan G,Dineshkumar K,Yang YH(2017)Microbial exopolysaccharide-mediated synthesis and stabilization of metal nanoparticles.Crit Rev Microbiol 43(6):731-752
3.Cui JD,Zhang BZ(2011)Comparison of culture methods on exopolysaccharide production in the submerged culture of Cordyceps militaris and process optimization.Lett Appl Microbiol 52(2):123-128
4.Ibarburu I,Puertas AI,Berregi I et al(2015)Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.Int J Food Microbiol 214:54-62
5.Bounaix MS,Gabriel V,Robert H et al(2010)Characterization of glucan-producing Leuconostoc strains isolated from sourdough.Int J Food Microbiol 144(1):1-9
6.Du RP,Xing HW,Zhou ZJ et al(2017)Isolation,characterisation,and fermentation optimisation of glucansucrase producing Leuconostoc mesenteroides DRP105 from sauerkraut with improved preservation stability.Int J Food Sci Technol 52(12):2522-2530
7.Cui JD,Qiu JQ(2012)Production of extracellular water insoluble polysaccharide from Pseudomonas sp.J Agric Food Chem60(19):4865-4871
8.Baruah R,Maina NH,Katina K et al(2017)Functional food applications of dextran from Weissella cibaria RBA12 from pummelo(Citrus maxima).Int J Food Microbiol 242:124-131
9.Schmid J(2018)Recent insights in microbial exopolysaccharide biosynthesis and engineering strategies.Curr Opin Biotechnol53:130-136
10.Zarour K,Llamas MG,Prieto A et al(2017)Rheology and bioactivity of high molecular weight dextrans synthesised by lactic acid bacteria.Carbohydr Polym 174:646-657
11.Kanmani P,Satish KR,Yuvaraj N et al(2011)Production and purifi cation of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro.Bioresour Technol 102(7):4827-4833
12.Maina NH,Tenkanen M,Maaheimo H et al(2008)NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa.Carbohydr Res 343(9):1446-1455
13.Leathers TD,Nunnally MS,Cote GL(2009)Modifi cation of alternan by dextranase.Biotechnol Lett 31(2):289-293
14.Yuliani E,Imai T,Teeka J et al(2011)Exopolysaccharide production from sweet potato-shochu distillery wastewater by Lactobacillus sakei CY1.Biotechnol Biotechnol Equip 25(2):2329-2333
15.Robijn GW,Van den Berg DJC,Haas H et al(1995)Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0?1.Carbohydr Res 276(1):117-136
16.Nácher-Vázquez M,Ballesteros N,Canalesáet al(2015)Dextrans produced by lactic acid bacteria exhibit antiviral and immunomodulatory activity against salmonid viruses.Carbohydr Polym124:292-301
17.Yang YP,Peng Q,Guo YY et al(2015)Isolation and characterization of dextran produced by Leuconostoc citreum NM105 from manchurian sauerkraut.Carbohydr Polym 133:365-372
18.Ai LZ,Guo QB,Ding HH et al(2016)Structure characterization of exopolysaccharides from Lactobacillus casei LC2W from skim milk.Food Hydrocoll 56:134-143
19.Trabelsi I,Slima SB,Chaabane H et al(2015)Purifi cation and characterization of a novel exopolysaccharides produced by Lactobacillus sp.Ca6.Int J Biol Macromol 74:541-546
20.Kavitake D,Devi PB,Singh SP et al(2016)Characterization of a novel galactan produced by Weissella confusa KR780676 from an acidic fermented food.Int J Biol Macromol 86:681-689
21.Saravanan C,Shetty PKH(2016)Isolation and characterization of exopolysaccharide from Leuconostoc lactis KC117496 isolated from idli batter.Int J Biol Macromol 90:100-106
22.Van den Berg DJC,Robijn GW,Janssen AC et al(1995)Production of a novel extracellular polysaccharide by Lactobacillus sake 0-1 and characterization of the polysaccharide.Appl Environ Microbiol 61(8):2840-2844
23.Wang J,Zhao X,Tian Z et al(2015)Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11isolated from Tibet Kefi r.Carbohydr Polym 125:16-25
24.Miao M,Bai AJ,Jiang B et al(2014)Characterisation of a novel water-soluble polysaccharide from Leuconostoc citreum SK24.002.Food Hydrocoll 36:265-272
25.Ahmed RZ,Siddiqui K,Arman M et al(2012)Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3.Carbohydr Polym 90(1):441-446
26.Du RP,Xing HW,Yang YP et al(2017)Optimization,purifi cation and structural characterization of a dextran produced by L.mesenteroides isolated from Chinese sauerkraut.Carbohydr Polym174:409-416
27.Bounaix MS,Gabriel V,Morel S et al(2009)Biodiversity of exopolysaccharides produced from sucrose by sourdough lactic acid bacteria.J Agric Food Chem 57(22):10889-10897
28.Freitas F,Alves VD,Reis MAM(2011)Advances in bacterial exopolysaccharides:from production to biotechnological applications.Trends Biotechnol 29(8):388-398
29.Lacaze G,Wick M,Cappelle S(2007)Emerging fermentation technologies:development of novel sourdoughs.Food Microbiol24(2):155-160
30.Tian YT,Zhao YT,Zeng HL et al(2016)Structural characterization of a novel neutral polysaccharide from Lentinus giganteus and its antitumor activity through inducing apoptosis.Carbohydr Polym 154:231-240
31.Sun YX,Liu JC,Yang XD et al(2010)Purifi cation,structural analysis and hydroxyl radical-scavenging capacity of a polysaccharide from the fruiting bodies of Russula virescens.Process Biochem 45(6):874-879
32.He YL,Ye M,Du ZZ et al(2014)Purifi cation,characterization and promoting eff ect on wound healing of an exopolysaccharide from Lachnum YM405.Carbohydr Polym 105:169-176
33.Shao L,Wu ZJ,Zhang H et al(2014)Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.Carbohydr Polym 107:51-56
34.Seymour FR,Knapp RD,Bishop SH(1976)Determination of the structure of dextran by 13 C nuclear magnetic resonance spectroscopy.Carbohydr Res 51(2):179-194
35.Uzochukwu S,Balogh E,Loefl er RT et al(2001)Structural analysis by 13 C-nuclear magnetic resonance spectroscopy of glucans elaborated by gum-producing bacteria isolated from palm wine.Food Chem 73(2):225-233
36.Purama RK,Goswami P,Khan AT et al(2009)Structural analysis and properties of dextran produced by Leuconostoc mesenteroides NRRL B-640.Carbohydr Polym 76(1):30-35
37.Li CC,Li W,Chen XH et al(2014)Microbiological,physicochemical and rheological properties of fermented soymilk produced with exopolysaccharide(EPS)producing lactic acid bacteria strains.LWT Food Sci Technol 57(2):477-485