无花果中维生素C含量的测定
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摘要
目的采用分光光度法测定无花果和无花果干中维生素C的含量。方法选取一定浓度维生素C标准液进行波长扫描,确定维生素C的最大吸收波长,将待测样品匀浆处理后在此波长下进行分光光度测定。结果维生素C的最大吸收波长为520 nm,校准曲线方程Y=0.0208X+0.001(r~2=0.9998),无花果和无花干果中维生素C含量分别为69.08 mg/100 g和64.06 mg/100 g,加标回收实验中回收率均在90%以上。结论无花果中含有较高的维生素C,且无花果维生素C含量高于无花果干中维生素C含量。
Objective To determine the content of vitamin C in Ficus carica L. and dry figs. Methods The certain concentration vitamin C standard solution was used for wavelength scanning to determine the maximum absorption wavelength of vitamin C. The homogenized sample was determined by spectrophotometry at this wavelength. Results The maximum absorption wavelength of vitamin C was 520 nm, the calibration curve equation was Y=0.0208X+0.001(r~2=0.9998), and the vitamin C content in Ficus carica and dried Ficus carica was 69.08 mg/100 g and 64.06 mg/100 g. The recoveries were above 90%. Conclusion Ficus carica L. has higher vitamin C content, and the vitamin C content in Ficus carica is higher than dry figs.
Objective To determine the content of vitamin C in Ficus carica L. and dry figs. Methods The certain concentration vitamin C standard solution was used for wavelength scanning to determine the maximum absorption wavelength of vitamin C. The homogenized sample was determined by spectrophotometry at this wavelength. Results The maximum absorption wavelength of vitamin C was 520 nm, the calibration curve equation was Y=0.0208X+0.001(r~2=0.9998), and the vitamin C content in Ficus carica and dried Ficus carica was 69.08 mg/100 g and 64.06 mg/100 g. The recoveries were above 90%. Conclusion Ficus carica L. has higher vitamin C content, and the vitamin C content in Ficus carica is higher than dry figs.
引文
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[19]张彩芳,李翠翠.番茄汁饮料维生素C稳定性的研究[J].粮食与食品工业,2018,25(4):25-30.Zhang CF,Li CC.Study on stability of vitamin C in tomato juice beverage[J].Cere Food Ind,2018,25(4):25-30.
[20]杨丽,麦振龙,朱良,等.果蔬中维生素C含量的测定方法比较与优化[J].安徽农业科学,2018,46(22):232-233,236.Yang L,Mai ZL,Zhu L,et al.Comparison and optimization of determination methods of vitamin C content in fruits and vegetables[J].JAnhui Agric Sci,2018,46(22):232-233,236.
[21]邱清莲,吴斯燕,刘光兰,等.含葡萄籽提取物的维生素C的测定[J].食品安全质量检测学报,2018,9(1):39-44.Qiu QL,Wu SY,Liu G L,et al.Determination of vitamin C in grape seed extraction[J].J Food Saf Qual,2018,9(1):39-44.
[22]杨艳.分光光度法测定苦丁茶中维生素C的含量[J].广州化工,2010,38(10):161-162.Yang Y.Determination of vitamin C in Iletlatifolia thunb with spectrophotography[J].Guangdong Chem Ind,2010,38(10):161-162.
[2]马艳弘,田丽敏,孙小华,等.无花果酶解制汁工艺优化及抗氧化活性[J].食品研究与开发,2019,40(1):111-117.Ma YH,Tian LM,Sun XH,et al.Optimization of enzymatic hydrolysis for production of fig juice and antioxidant activity[J].Food Res Dev,2019,40(1):111-117.
[3]张锦华,徐蔓,白宝清,等.响应面法优化提取无花果干果中多酚和总黄酮物质及其抗氧化活性[J].食品工业科技,2018,39(16):183-190,212.Zhang JH,Xu M,Bai BQ,et al.Optimization of extraction of polyphenols and total flavonoids from dried figs by response surface methodology and antioxidant activity analysis[J].Sci Technol Food Ind,2018,39(16):183-190,212.
[4]崔鹏,陈志冉,史雷革.微波提取无花果叶中的总黄酮及其体外抗氧化活性研究[J].食品研究与开发,2018,39(1):41-45.Cui P,Chen ZR,Shi LG,et al.Microwave extraction and free radical scavenging activity of total flavonoids from Ficus carica leaves[J].Food Res Dev,2018,39(1):41-45.
[5]徐蔓,张锦华,白宝清.无花果干果不同溶剂提取物及其抗氧化活性研究[J].山西农业科学,2017,45(9):1430-1434.Xu M,Zhang JH,Bai BQ,et al.Study on different solvent extracts of fig dried fruit and its antioxidative activity[J].J Shanxi Agric Sci,2017,45(9):1430-1434.
[6]秦丹丹,张生万,郭萌,等.干燥方式对无花果酚类物质及其抗氧化活性的影响[J].食品科学,2018,39(9):102-107.Qin DD,Zhang SW,Guo M,et al.Effect of drying methods on polyphenol composition and antioxidant activities of figs(Ficus carica L.)[J].Food Sci,2018,39(9):102-107.
[7]白冬红,卢昊,辛力,等.几种无花果干制产品抗氧化性的测定[J].山东农业科学,2017,49(2):64-66,71.Bai DH,Lu H,Xin L,et al.Determination of antioxidant ability of several dried fig products[J].J Shandong Agric Sci,2017,49(2):64-66,71.
[8]马丽,白淑荣,韩占友,等.果蔬中总多酚和维生素C测定方法优化的研究[J].食品安全质量检测学报,2015,6(4):1492-1497.Ma L,Bai SR,Han ZY,et al.Study on optimized determination of total polyphenols and vitamin C[J].J Food Saf Qual,2015,6(4):1492-1497.
[9]李文婕,杨小明,张赫男,等.无花果多糖的纯化及其抗氧化活性研究[J].食品工业科技,2014,35(14):161-165.Li WJ,Yang XM,Zhang HN,et al.Study on purification and antioxidation of polysaccharides from Ficus carica L.[J].Sci Technol Food Ind,2014,35(14):161-165.
[10]汤慧民.无花果的抗氧化研究[J].食品工业,2012,33(11):135-137.Tang HM.Study on antioxidation of Ficus carica L.[J].Food Ind,2012,33(11):135-137.
[11]罗志辉,陈丽娜,陈冠丹,等.新型紫外可见光法测定沙田柚中维生素C的含量[J].食品安全质量检测学报,2018,9(10):2321-2325.Luo ZH,Chen LN,Chen GD,et al.A novel UV-visible method for the detection of vitamin C in Shatian pumelo[J].J Food Saf Qual,2018,9(10):2321-2325.
[12]张泽俊,沙坤,马雯.无花果叶不同溶剂提取物抗氧化活性的比较研究[J].安徽农业科学,2011,39(12):6981-6982,7010.Zhang ZJ,Sha K,Ma W.Study on antioxidative activity of the different solvent extracts from Ficus carica L.leaves[J].J Anhui Agric Sci,2011,39(12):6981-6982,7010.
[13]邱松山,周天,姜翠翠.无花果粗多糖提取工艺及抗氧化活性研究[J].食品与机械,2011,27(1):40-42.Qiu SS,Zhou T,Jiang CC.Preliminary study on extracting technology and antioxidant acitvity of total polysaccharide from Ficus carica L.[J].Food Mach,2011,27(1):40-42.
[14]周爱琴,李春丽,王然,等.无花果果实发育形态学观察[J].北京农学院学报,2016,31(4):17-20.Zhou AQ,Li CL,Wang R,et al.Morphological observation on fig fruit development[J].J Beijing Univ Agric,2016,31(4):17-20.
[15]张小曼,马银海,袁琳,等.无花果皮红色素提取及其抗氧化性[J].食品研究与开发,2010,31(12):281-284.Zhang XM,Ma YH,Yuan L,et al.Study on resin extraction and antioxidant activity of red pigment from pericapr of Ficus carica L.[J].Food Res Dev,2010,31(12):281-284.
[16]Han QQ,Shen TT,Wang F,et al.Preventive and therapeutic potential of vitamin C in mental disorders[J].Current Med Sci,2018,38(1):1-10.
[17]Ang A,Pullar JM,Currie MJ,et al.Vitamin C and immune cell function in inflammation and cancer[J].Biochem Soc Trans,2018,46(5):1147-1159.
[18]颜道民,尹金晶,唐晋文,等.鲜食无花果贮藏保鲜技术研究进展[J].食品安全质量检测学报,2019,10(9):2462-2467.Yan DM,Yin JJ,Tang JW.Research advances on storage and fresh-keeping technology of fresh Ficus carica L.[J].J Food Saf Qual,2019,10(9):2462-2467.
[19]张彩芳,李翠翠.番茄汁饮料维生素C稳定性的研究[J].粮食与食品工业,2018,25(4):25-30.Zhang CF,Li CC.Study on stability of vitamin C in tomato juice beverage[J].Cere Food Ind,2018,25(4):25-30.
[20]杨丽,麦振龙,朱良,等.果蔬中维生素C含量的测定方法比较与优化[J].安徽农业科学,2018,46(22):232-233,236.Yang L,Mai ZL,Zhu L,et al.Comparison and optimization of determination methods of vitamin C content in fruits and vegetables[J].JAnhui Agric Sci,2018,46(22):232-233,236.
[21]邱清莲,吴斯燕,刘光兰,等.含葡萄籽提取物的维生素C的测定[J].食品安全质量检测学报,2018,9(1):39-44.Qiu QL,Wu SY,Liu G L,et al.Determination of vitamin C in grape seed extraction[J].J Food Saf Qual,2018,9(1):39-44.
[22]杨艳.分光光度法测定苦丁茶中维生素C的含量[J].广州化工,2010,38(10):161-162.Yang Y.Determination of vitamin C in Iletlatifolia thunb with spectrophotography[J].Guangdong Chem Ind,2010,38(10):161-162.