磁核碳点制备及其主要影响因素分析
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摘要
为富集碳点荧光信号,提高碳点在超痕量检测应用的灵敏性,用壳聚糖包埋磁核的纳米材料,通过水热法原位生成磁核碳点并进行表征,利用正交试验对其制备条件进行分析。试验结果表明,通过控制碳点生成的反应温度、反应时间和碳源浓度,可制备荧光性能优异,并具有磁分离效果的碳点,其中反应温度是磁核碳点生成的关键影响因素(P=0.015)。该碳点的成功制备有利于提高检测灵敏度,应用前景广阔。
To enrich the fluorescence of carbon dots and improve the sensitivity of carbon dots in ultratrace detection,the magnetic-core carbon dots(Mc-CDs) were in-situ synthesized using the nano material of chitosan-packaged magnetic cores through hydrothermal method in this paper.The as-synthesized Mc-CDs were characterized.The conditions of Mc-CDs preparation were analyzed by orthogonal test.The results showed that the Mc-CDs can be successfully fabricated with excellent fluorescent properties under controlling of the temperature,reaction time and concentrations of carbon source of magnetic-core,which had eminent effect of magnetic separation.The reaction temperature played a key role in synthesis of McCDs(P = 0.015).The successful synthesis of Mc-CDs improved the sensitivity of CDs in detection,endowing Mc-CDs with wide prospect of applications.
To enrich the fluorescence of carbon dots and improve the sensitivity of carbon dots in ultratrace detection,the magnetic-core carbon dots(Mc-CDs) were in-situ synthesized using the nano material of chitosan-packaged magnetic cores through hydrothermal method in this paper.The as-synthesized Mc-CDs were characterized.The conditions of Mc-CDs preparation were analyzed by orthogonal test.The results showed that the Mc-CDs can be successfully fabricated with excellent fluorescent properties under controlling of the temperature,reaction time and concentrations of carbon source of magnetic-core,which had eminent effect of magnetic separation.The reaction temperature played a key role in synthesis of McCDs(P = 0.015).The successful synthesis of Mc-CDs improved the sensitivity of CDs in detection,endowing Mc-CDs with wide prospect of applications.
引文
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[2]Esteves da Silva JCG,Goncalves HMR.Analytical and bioanalytical applications of carbon dots[J].TracTrends in Analytical Chemistry,2011,30(8):1327-1336.
[3]Mosconi D,Mazzier D,Silvestrini S,et al.Synthesis and Photochemical Applications of Processable Polymers Enclosing Photo luminescent Carbon Quantum Dots[J].ACS Nano,2015,9(4):4156-4164.
[4]Hou J,Li H,Wang L,et al.Rapid microwave-assisted synthesis of molecularly imprinted polymers on carbon quantum dots for fluorescent sensing of tetracycline in milk[J].Talanta,2016(146):34-40.
[5]Weng CI,Chang HT,Lin CH,et al.One-step synthesis of biofunctional carbon quantum dots for bacterial labeling[J].Biosens Bioelectron,2015(68):1-6.
[6]Mehta VN,Jha S,Basu H,et al.One-step hydrothermal approach to fabricate carbon dots from apple juice for imaging of mycobacterium and fungal cells[J].Sensors and Actuators B:Chemical,2015(213):434-443.
[7]Pierrat P,Wang R,Kereselidze D,et al.Efficient in vitro and in vivo pulmonary delivery of nucleic acid by carbon dot-based nanocarriers[J].Biomaterials,2015(51):290-302.
[8]He J,Zhang H,Zou J,et al.Carbon dots-based fluorescent probe for"off-on"sensing of Hg(II)and(I)[J].Biosens Bioelectron,2016(79):531-535.
[9]Chen Y,Wu Y,Weng B,et al.Facile synthesis of nitrogen and sulfur co-doped carbon dots and application for Fe(III)ions detection and cell imaging[J].Sensors and Actuators B:Chemical,2016(223):689-696.
[10]Ye ZQ,Tang R,Wu H,et al.Preparation of europium complex-conjugated carbon dots for ratiometric fluorescence detection of copper(II)ions[J].New Journal of Chemistry,2014,38(12):5721-5726.
[11]Shereema RM,Sankar V,Raghu KG,et al.One Step Green Synthesis of Carbon Quantum Dots and Its Application Towards the Bioelectroanalytical and Biolabeling Studies[J].Electrochimica Acta,2015(182):588-595.
[12]Sharma R,Ragavan KV,Thakur MS,et al.Recent advances in nanoparticle based aptasensors for food contaminants[J].Biosens Bioelectron,2015(74):612-627.
[13]Gu HW,Xu KM,Xu CJ,et al.Biofunctional magnetic nanoparticles for protein separation and pathogen detection[J].Chemical Communications,2006(9):941-949.
[14]Shi Y,Pan Y,Zhong J,et al.Facile synthesis of gadolinium(III)chelates functionalized carbon quantum dots for fluorescence and magnetic resonance dual-modal bioimaging[J].Carbon,2015(93):742-750.
[15]Cao M,Li Z,Wang J,et al.Food related applications of magnetic iron oxide nanoparticles:Enzyme immobilization,protein purification,and food analysis[J].Trends in Food Science&Technology,2012;27(1):47-56.
[16]Yao W,Rao PH,Du YT,et al.Synthesis of magnetic silica with quaternary ammonium salt and its application for chromium(VI)removal[J].Desalination And Water Treatment,2015,55(1):173-182.
[17]Purbia R,Paria S.A simple turn on fluorescent sensor for the selective detection of thiamine using coconut water derived luminescent carbon dots[J].Biosens Bioelectron,2016(79):467-475.