摘要
快速碱化因子(RALF)是一种能够参与植物发育调控的植物肽激素,在真菌中也广泛存在并且影响真菌对寄主的侵染状况。通过外源添加酸碱来明确pH值对玉米大斑菌生长的影响,使用实时荧光定量PCR技术分析玉米大斑病菌中StRALF基因在外源添加酸、碱时表达量的变化。运用预测网站对克隆得到的StRALF基因编码区蛋白序列的基本性质进行分析。结果表明,玉米大斑瓋菌在碱性环境下生长状态较好。碱性条件下,StRALF基因比酸性条件下基因表达量高。在侵染寄主过程中,96 h及以后表达量达到最大。经预测分析,初步确定其为分泌蛋白或膜蛋白。在弱碱性条件下,StRALF基因表达量较高并参与侵染过程及侵染后的生长阶段。
Rapid alkalization factor(RALF) is a kind of plant peptide hormone that can participate in the regula-tion of plant development. It exists widely in fungi and affects the infectivity of fungi. In this paper, the effects of dif-ferent pH values on the growth of Setosphaeria turcica were determined by adding acid and alkali to cultural medi-um. The changes in the expression of StRALF gene in S. turcica were analyzed by real-time fluorescence quantita-tive PCR technology. The basic properties of StRALF gene coding region protein were analyzed at prediction web-site. The results showed that S. turcica grew better in alkaline environment; and in alkaline condition, the expression level of StRALF gene was higher than that in acid condition. During the process of S. turcica infection to host, the ex-pression of StRALF gene reached the maximum after 96 h, and it was preliminarily predicted as secretory protein or membrane protein. In total, under weak alkaline condition, StRALF gene was up-regulated and then involved in the infection process and growth period. This study can provide a reference for the further research on the action and ac-tion mechanism of RALF in S. turcica.
引文
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