摘要
从新疆盐碱土中分离纯化得到一株高效解磷菌PS-3,为改善盐碱地磷素供应提供菌种资源。采用单因素和正交试验,对菌株PS-3的发酵条件和溶磷能力进行分析。结果表明,菌株PS-3较适发酵条件为温度35℃、pH 7和盐浓度1%,其对碳、氮源的利用顺序依次为葡萄糖>麦芽糖>果糖>蔗糖>乳糖,硝酸铵>硝酸钠>氯化铵>硫酸铵>尿素,菌株PS-3最适溶磷条件为2%葡萄糖、0.01%硝酸铵、温度35℃、初始pH 7.5、盐浓度2 g/L、接种量4%,在此条件下,其对磷酸三钙的溶解量可达100 2.95mg/L。该菌株具有一定耐盐性和良好的溶磷能力,在生物溶磷方面具有较好的应用潜力。
The aim of the present work is to isolate a highly effective phosphate-solubilizing strain PS-3 for improving phosphorus supplyof saline-alkali soil in Xinjiang. The single factor experiment and the orthogonal experiment were used to analyze the fermentation conditionsand the phosphate-dissolving capability of strain PS-3. The results showed that the appropriate fermentation conditions of the strain PS-3 weretemperature 35℃,pH 7 and salt concentration 1%. The order of the strain utilizing carbon and nitrogen was glucose > maltose > fructose >sucrose > lactose,and ammonium nitrate > sodium nitrate > ammonium chloride > ammonium sulfate > urea,respectively. The optimalconditions for the solubilization of strain PS-3 were 2% glucose,0.01% ammonium nitrate,temperature 35℃,initial pH 7.5,salt content 2 g/L,and inoculation amount 4%. The phosphate-solubilizing capability reached the level of 1 002.95 mg/L under this condition. The strain PS-3 istolerant to salt stress and has desired phosphate-solubilizing capacity,and thus has a better potential for microbial inoculants with phosphorussolubilizing ability.
引文
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