焙制程度对壁虎抑制H_(22)荷瘤小鼠肿瘤生长活性的影响
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摘要
研究焙制程度对壁虎抗肿瘤活性的影响。利用传统烘干结合瓦片焙干的方法制备壁虎不同程度焙制品,检测酥脆度并进行感官评价,按药典方法检测含水量和水溶性浸出物含量,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法检测可溶性蛋白质多样性;制备H_(22)荷瘤小鼠模型,检测壁虎不同程度焙制品抑瘤效果及免疫器官指数,并评价小鼠的生存质量,相关动物实验获得北京中医药大学伦理学委员会批准。随着焙制程度的加深,壁虎可溶性蛋白质分子量大小有显著差异并且蛋白质浓度降低,抗肿瘤活性由高到低顺序为烘干未焙制品(34.11%)、焙至微黄品(29.14%)、焙至焦黑品(28.43%)和焙至焦黄品(21.98%),焙制品感官评价优于烘干未焙制品。结果提示,焙制提升感官评价,但会降低壁虎抗肿瘤活性;以疗效为指标,烘干至含水量7.71%为佳。
This study was designed to investigate the effect on tumor growth inhibition activity of lizards(Gekkoswinhonis Guenther) with different extent of broiling. Samples were prepared by a traditional drying method combined with broiling on clay tiles. Four groups of samples were all dried before broiling. Group A was without broiling; group B was mildly broiled; group C was moderately broiled; and group D was heavily broiled. Crispiness was detected by the sizes of the generated fragments of different groups and crispiness increased with broiling.Sensory evaluation of vision and olfaction was performed, and scores were generated by evaluators. Moderately broiled group had the highest total score in sensory evaluation. Water content and content of water-soluble extracts were detected according to Chinese Pharmacopoeia. With the increasing broiling extent, content of water-soluble extracts increased while water content decreased. Soluble protein concentration was detected by bicinchoninic acid(BCA) kit and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) with the same crude drug content. Soluble protein concentration decreased with the increasing broiling extent. With equal loading of proteins at the same concentration, soluble protein diversity was detected by SDS-PAGE. Band difference was marked by red boxes. Soluble protein molecule weights showed significant difference with the increasing broiling extent. H_(22) tumor-bearing mice model was established and used to detect tumor growth inhibition rate and immune organ index. Life quality of mice was evaluated. Mice treated with Gekkoswinhonis Guenther had better appetites and higher average weights compared with positive control group treated with fluorouracil(5-FU). Animal experiments were approved by the Ethics Committee of Beijing University of Chinese Medicine. Group A had the highest tumor growth inhibition rate(34.11%), followed by Group B(29.14%) and Group D(28.43%), Group C(21.98%)had the lowest tumor growth inhibition rate, but sensory evaluation was on the contrary. These results indicated that moderately broiling improved sensory evaluation but reduced the tumor growth inhibition activity of Gekko‐swinhonis Guenther. The best tumor growth inhibition activity appeared when water content was 7.71%.
This study was designed to investigate the effect on tumor growth inhibition activity of lizards(Gekkoswinhonis Guenther) with different extent of broiling. Samples were prepared by a traditional drying method combined with broiling on clay tiles. Four groups of samples were all dried before broiling. Group A was without broiling; group B was mildly broiled; group C was moderately broiled; and group D was heavily broiled. Crispiness was detected by the sizes of the generated fragments of different groups and crispiness increased with broiling.Sensory evaluation of vision and olfaction was performed, and scores were generated by evaluators. Moderately broiled group had the highest total score in sensory evaluation. Water content and content of water-soluble extracts were detected according to Chinese Pharmacopoeia. With the increasing broiling extent, content of water-soluble extracts increased while water content decreased. Soluble protein concentration was detected by bicinchoninic acid(BCA) kit and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) with the same crude drug content. Soluble protein concentration decreased with the increasing broiling extent. With equal loading of proteins at the same concentration, soluble protein diversity was detected by SDS-PAGE. Band difference was marked by red boxes. Soluble protein molecule weights showed significant difference with the increasing broiling extent. H_(22) tumor-bearing mice model was established and used to detect tumor growth inhibition rate and immune organ index. Life quality of mice was evaluated. Mice treated with Gekkoswinhonis Guenther had better appetites and higher average weights compared with positive control group treated with fluorouracil(5-FU). Animal experiments were approved by the Ethics Committee of Beijing University of Chinese Medicine. Group A had the highest tumor growth inhibition rate(34.11%), followed by Group B(29.14%) and Group D(28.43%), Group C(21.98%)had the lowest tumor growth inhibition rate, but sensory evaluation was on the contrary. These results indicated that moderately broiling improved sensory evaluation but reduced the tumor growth inhibition activity of Gekko‐swinhonis Guenther. The best tumor growth inhibition activity appeared when water content was 7.71%.
引文
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[16] Hou XN. Anti-tumor Activity Study of Gecko(壁虎抗肿瘤研究)[D]. Beijing:Beijing University of Chinese Medicine, 2008.
[2] Li SZ. Compendium of Materia Medica(本草纲目)[M]. Beijing:People's Medical Publishing House,1982:2389.
[3] Jiang GX, Wang CM, Geng D. Research progress and mechanism research on anti-tumor material finding from Gecko[J]. J Tradit Chin Med(中华中医药杂志),2013, 28:1037-1040.
[4] Chen G, Li W. Editorial preface for targeted cancer therapy[J].Acta Pharm Sin B, 2018, 8:501-502.
[5] Cui YL. Doctor Li Jiansheng's experience on treatment ofesophageal carcinoma[J]. Beijing J Tradit Chin Med(北京中医),2005, 24:269-270.
[6] Xu MS, Li ZB. Yiqi Sanjie decoction and Xiaojie powder in the treatment of 80 cases of a dvanced esophageal cancer pain[J].Shanxi J Tradit Chin Med(陕西中医),2003, 24:612-613.
[7] Qiao YS,Zhou SH. Experience of diagnosing and treating cerebral tumor from Zhou Chang'an[J]. China Med Pharm(中国医药科学),2013, 3:99-101.
[8] Luo HD. Three treatments and tests of neck tumor[J]. J Sichuan Tradit Chin Med(四川中医),2001,19:39-40.
[9] Hou XN, Geng D, Cai A, et al. Comparative research on anticancer activity between fresh and processed Gekkosubpalmatus[J]. J Chin Med Mater(中药材),2008, 31:957-959.
[10] Drug Administration of the People's Republic of China. National Standard for Processing Traditional Chinese Medicine(全国中药炮制规范)[M]. Beijing:People's Medical Publishing House,1988:332.
[11] Zhou GR. Clinical observation on treatment of 8 cases of fistula with Gecko powder[J]. Chin J Ethnomed Ethnopharm(中国民族民间医药杂志),2000, 44:137.
[12] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China(中华人民共和国药典:四部)[S].Beijing:China Medical Science Press, 2015.
[13] Wang BB, Wang XH, Liu K, et al. Enhanced radiosensitivity of H22 ascitic tumor to 12C6+ions radiation in ultra-filtration extract mixture from Astragalus mongholicus-treated mice[J]. Acta Pharm Sin(药学学报),2015, 50:1596-1602.
[14] Fang XY. Medical Experimental Zoology(医学实验动物学)[M]. Beijing:People's Medical Publishing House, 1995:104-116.
[15] Ouyang LD, Ma L, Xiao XH. Analysis on the status quo and countermeasures of processing animal medicine of traditional Chinese medicine[J]. Chin Tradit Pat Med(中成药),2017, 39:1034-1037.
[16] Hou XN. Anti-tumor Activity Study of Gecko(壁虎抗肿瘤研究)[D]. Beijing:Beijing University of Chinese Medicine, 2008.