补肾养血煎剂对特应性皮炎豚鼠模型肾上腺脏器系数影响的实验研究
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摘要
目的探讨补肾养血煎剂对特应性皮炎豚鼠模型肾上腺脏器系数影响的实验研究。方法通过丙酮激发豚鼠,模拟特应性皮炎皮损,分别造成A组急性特应性皮炎模型与B组建立亚急性/慢性特应性皮炎模型,2组均分为模型对照组、阳性药物对照组、中药高剂量组、中药低剂量组,分别应用生理盐水、盐酸西替利嗪片、补肾养血煎剂治疗。对豚鼠模型肾上腺脏器系数进行测定,摘取肾上腺并称重,计算脏器系数。结果特应性皮炎豚鼠A、B模型组与空白对照组比较,肾上腺系数降低(P <0.01)。与A、B模型组比较,A、B阳性药物组、中药高剂量组、中药低剂量组肾上腺系数均明显增高(P <0.01)。A组阳性药物组与中药高剂量组比较,中药高剂量可提高肾上腺系数(P <0.05)。结论补肾养血煎剂可以提高豚鼠模型肾上腺脏器系数,对实验性特应性皮炎有治疗作用。
Objective To investigate the effect of Bushen Yangxue decoction on adrenal organ coefficient in guinea pigs with atopic dermatitis. Methods Guinea pigs were stimulated by acetone to simulate atopic dermatitis lesions.Subacute/chronic atopic dermatitis models were established in group A and group B respectively. The two groups were divided into model control group, positive drug control group, high-dose group and low-dose group of traditional Chinese medicine. They were treated with saline, cetirizine hydrochloride tablets and Bushen Yangxue decoction respectively. The adrenal organ coefficients of guinea pigs were measured, and the adrenal glands were extracted and weighed, and the organ coefficients were calculated. Results The adrenal coefficient of model A and B guinea pigs with atopic dermatitis was lower than that of blank control group(P < 0.01). Compared with model group A and B,the adrenal coefficient of positive drug group A and B, high dose group and low dose group of traditional Chinese medicine increased significantly(P < 0.01). Comparison between positive drug A group and high dose group of traditional Chinese medicine, high dose of traditional Chinese Medicine can increase adrenal coefficient(P < 0.05).Conclusion Bushen Yangxue decoction can improve the adrenal organ coefficient of guinea pig model and has therapeutic effect on experimental atopic dermatitis.
Objective To investigate the effect of Bushen Yangxue decoction on adrenal organ coefficient in guinea pigs with atopic dermatitis. Methods Guinea pigs were stimulated by acetone to simulate atopic dermatitis lesions.Subacute/chronic atopic dermatitis models were established in group A and group B respectively. The two groups were divided into model control group, positive drug control group, high-dose group and low-dose group of traditional Chinese medicine. They were treated with saline, cetirizine hydrochloride tablets and Bushen Yangxue decoction respectively. The adrenal organ coefficients of guinea pigs were measured, and the adrenal glands were extracted and weighed, and the organ coefficients were calculated. Results The adrenal coefficient of model A and B guinea pigs with atopic dermatitis was lower than that of blank control group(P < 0.01). Compared with model group A and B,the adrenal coefficient of positive drug group A and B, high dose group and low dose group of traditional Chinese medicine increased significantly(P < 0.01). Comparison between positive drug A group and high dose group of traditional Chinese medicine, high dose of traditional Chinese Medicine can increase adrenal coefficient(P < 0.05).Conclusion Bushen Yangxue decoction can improve the adrenal organ coefficient of guinea pig model and has therapeutic effect on experimental atopic dermatitis.
引文
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[15]杨连卿.滋阴药、温肾阳中药对大鼠下丘脑-垂体-肾上腺皮质轴受抑模型的作用-肾上腺皮质束状带超微结构观察[J].中西医结合杂志, 1983, 3(6):353.
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[18]宋显利.养血祛风饮治疗特应性皮炎的临床观察及对IgE的影响[D].哈尔滨:黑龙江中医药大学, 2017.
[2]阮红石.中药外用药治疗特应性皮炎的Meta分析及用药规律研究[D].广州:广州中医药大学, 2014.
[3]郑益志,余土根,朱金土,等.异位性皮炎中医辨证分型与Thl/Th2细胞因子表达水平的相关性[J].中国中西医结合杂志, 2010, 30(1):102.
[4]陈梦娇,丁佩军,张慧敏.中医药对特应性皮炎免疫调节作用的研究进展[J].现代中西医结合杂志, 2016, 25(24):2734-2736.
[5]张志斌,郑金生.《本草纲目》整理研究的再思考[J].中医杂志, 2016, 57(22):1896-1900.
[6]韩首章,曹旭,韩翔.病理指导下运用中药治疗寻常性银屑病210例[J].辽宁中医杂志, 2013, 40(8):1628.
[7]王海波,刘海春,王振清.健脾安神中药治疗特应性皮炎[J].吉林中医药, 2014, 34(12):1255-1257.
[8]赵辩.中国临床皮肤病学[M].南京:江苏科学技术出版社,2012:717.
[9]刘国栋,马鑫,马拴全,等.中药配合冷喷枸地氯雷他定治疗激素依赖性皮炎[J].长春中医药大学学报, 2015, 31(4):824-826.
[10]瞿旻晔,袁晓琳,马健.特应性皮炎发病过程及其机制研究现状探讨[J].中国皮肤性病学杂志, 2015, 29(10):1085-1087.
[11] GALAL E E. Ef fect of glycyrrhetinic acid on salt and water metabolism. Brit Jphamacol, 1995(2):305.
[12]王敏,杨进,李岩.火针配合体针围刺治疗局限性神经性皮炎疗效观察[J].天津中医药, 2014, 33(8):475-477.
[13]饶琪,宋瑜.中医药治疗特应性皮炎研究进展[J].浙江中西医结合杂志, 2014, 24(7):651-654.
[14]赵巍.中药“喘可治”治疗特应性皮炎的实验与临床研究[D].广州:广州中医药大学, 2016.
[15]杨连卿.滋阴药、温肾阳中药对大鼠下丘脑-垂体-肾上腺皮质轴受抑模型的作用-肾上腺皮质束状带超微结构观察[J].中西医结合杂志, 1983, 3(6):353.
[16]蔡定芳.右归饮对大鼠下丘脑-垂体-肾上腺-胸腺轴抑制模型的影响[J].中国免疫学杂志, 1994, 10(4):236.
[17]蔡定芳,沈自尹,张玲娟,等.右归饮对皮质酮大鼠下丘脑单胺类递质含量以及体质量饮食摄水的影响.中国中西医结合杂志[J]. 1995, l5(l2):728-731.
[18]宋显利.养血祛风饮治疗特应性皮炎的临床观察及对IgE的影响[D].哈尔滨:黑龙江中医药大学, 2017.