MicroRNA-29a在结直肠癌组织中的表达及意义
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摘要
目的探讨micro RNA-29a(mi R-29a)在结直肠癌组织中的表达,以及mi R-29a反义寡核苷酸对结直肠癌细胞增殖和侵袭的影响。方法选取行手术治疗的初诊结直肠癌患者195例,利用实时荧光定量聚合酶链反应检测结直肠癌和癌旁组织中mi R-29a表达,培养人结直肠癌Lo Vo细胞,随机分为ASO-mi R-29a组、ASO-对照序列组和空白对照组,检测各组细胞中mi R-29a表达,MTT法检测各组细胞增殖能力,Transwell小室检测各组细胞迁移和侵袭能力。结果结直肠癌组织mi R-29a相对表达量为(1.93±0.19),癌旁组织为(1.26±0.12),两者比较差异有统计学意义(P <0.05);结直肠癌组织中mi R-29a相对表达量与TNM分期和淋巴结转移有关(P <0.05),多元线性回归分析结果显示,TNM分期和淋巴结转移是影响结直肠癌组织中mi R-29a表达的因素(P <0.05);ASO-mi R-29a组细胞中mi R-29a相对表达量低于ASO-对照序列组和空白对照组(P <0.05);MTT实验结果显示,ASO-miR-29a组与ASO-对照序列组和空白对照组相比吸光度值降低(P <0.05);与ASO-对照序列组和空白对照组比较,ASO-miR-29a组迁移细胞数和侵袭细胞数均减少(P <0.05)。结论 mi R-29a在结直肠癌组织中呈高表达,参与结直肠癌发生、进展过程,特异性抑制结直肠癌细胞中miR-29a表达可减少细胞增殖,抑制细胞迁移和侵袭能力。
Objective To investigate the expression of miRNA-29 a in colorectal cancer, and the effect of miRNA-29 a on proliferation and invasion of colorectal cancer cells. Methods A total of 195 cases of colorectal cancer were involved in this study. The expression of miRNA-29 a in colorectal cancer tissue and adjacent normal tissue was detected by real-time fluorescence quantitative PCR. Human colorectal cancer LoVo cell line was cultured.All cells were randomly divided into ASO-miRNA-29 a group, ASO-control sequence group and blank control group.MTT and Transwell assay were performed to detect the cell proliferation and invasion, respectively. Results The relative expression level of miRNA-29 a in the colorectal cancer tissues was increased when compared with adjacent tissues was [(1.93 ± 0.19) vs(1.26 ± 0.12), P < 0.05]. Multiple linear regression analysis suggested that increased expression level of miRNA-29 a in colorectal cancer tissues was correlated to TNM stage and lymph node metastasis(P < 0.05). The relative expression level of miRNA-29 a in ASO-miRNA-29 a group was lower than the ASO-control group and blank control group(P < 0.05). MTT and Transwell assay showed that silencing miRNA-29 a compromised cellular proliferation and invasive capability when compared with the ASO-control sequence group and blank control group(P < 0.05). Conclusions Enhanced expression of miRNA-29 a in colorectal cancer tissues may be involved in the carcinogenesis and progression of colorectal cancer.
Objective To investigate the expression of miRNA-29 a in colorectal cancer, and the effect of miRNA-29 a on proliferation and invasion of colorectal cancer cells. Methods A total of 195 cases of colorectal cancer were involved in this study. The expression of miRNA-29 a in colorectal cancer tissue and adjacent normal tissue was detected by real-time fluorescence quantitative PCR. Human colorectal cancer LoVo cell line was cultured.All cells were randomly divided into ASO-miRNA-29 a group, ASO-control sequence group and blank control group.MTT and Transwell assay were performed to detect the cell proliferation and invasion, respectively. Results The relative expression level of miRNA-29 a in the colorectal cancer tissues was increased when compared with adjacent tissues was [(1.93 ± 0.19) vs(1.26 ± 0.12), P < 0.05]. Multiple linear regression analysis suggested that increased expression level of miRNA-29 a in colorectal cancer tissues was correlated to TNM stage and lymph node metastasis(P < 0.05). The relative expression level of miRNA-29 a in ASO-miRNA-29 a group was lower than the ASO-control group and blank control group(P < 0.05). MTT and Transwell assay showed that silencing miRNA-29 a compromised cellular proliferation and invasive capability when compared with the ASO-control sequence group and blank control group(P < 0.05). Conclusions Enhanced expression of miRNA-29 a in colorectal cancer tissues may be involved in the carcinogenesis and progression of colorectal cancer.
引文
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[2]TAKAHASHI R U,PRIETO-VILA M,HIRONAKA A,et al.The role of extracellular vesicle microRNAs in cancer biology[J].Clin Chem Lab Med,2017,55(5):648-656.
[3]MAHATI S,XIAO L,YANG Y,et al.miRNA-29a suppresses growth and migration of hepatocellular carcinoma by regulating CLDN1[J].Biochem Biophys Res Commun,2017,486(3):732-737.
[4]奚卓,薛一雪,马珺,等.miRNA-29s家族对人脑胶质瘤干细胞生物学行为的影响[J].中国医科大学学报,2017,46(3):201-209.
[5]宋佳伦,杨琦.转录因子扭曲基因表达异常在早期子宫颈癌发病中的意义及对预后的影响[J].中国妇幼健康研究,2016,27(9):1066-1068.
[6]王建国,史春云,王建飞,等.miRNA-7对胃癌细胞SGC7901增殖、迁移及侵袭能力的影响及其与黏着斑激酶相关性研究[J].华中科技大学学报(医学版),2016,45(5):519-522.
[7]李增军,王海鹏,宋宝,等.RNA干扰沉默高迁移率族蛋白B1的表达对结直肠癌LoVo细胞增殖和侵袭的抑制作用[J].中华肿瘤杂志,2015,37(9):664-670.
[8]冯雅靖,王宁,方利文,等.1990年与2013年中国人群结直肠癌疾病负担分析[J].中华流行病学杂志,2016,37(6):768-772.
[9]傅佶泓.结直肠癌中差异表达miRNA的筛选与功能研究[D].上海:上海交通大学,2013.
[10]KEDMI M,SAS-CHEN A,YAREN Y.MicroRNAs and growth factors:an alliance propelling tumor progression[J].J Clin Med,2015,4(8):1578-1599.
[11]YAMADA A,HORIMATSU T,OKUGAWA Y,et al.Serum miRNA-21,miRNA-29a,and miRNA-125b are promising biomarkers for the early detection of colorectal neoplasia[J].Clin Cancer Res,2015,21(18):4234-4242.
[12]PEI Y F,LEI Y,LIU X Q.miR-29a promotes cell proliferation and EMT in breast cancer by targeting ten eleven translocation 1[J].Biochim Biophys Acta,2016,1862(11):2177-2185.
[13]KWON J J,WILLY J A,QUIRIN K A,et al.Novel role of miRNA-29a in pancreatic cancer autophagy and its therapeutic potential[J].Oncotarget,2016,7(44):71635-71650.
[14]李志华.miRNA-29家族调控乳腺癌形成、发展和转移分子机制的研究进展[J].肿瘤研究与临床,2016,28(7):490-493.