思茅松毛虫肠道真菌分离鉴定及水解酶活性初步研究
|
摘要
肠道微生物在昆虫的食物消化、免疫防御中发挥重要作用,但目前对昆虫肠道真菌了解不多。本研究以重要林业害虫—思茅松毛虫Dendrolimu kikuchii Matsumura为材料,分离鉴定其幼虫中的肠道真菌。采用传统微生物分离纯培养的方法从思茅松毛虫4龄幼虫肠道样品中分离肠道真菌,运用ITS序列分析鉴定,并对其产酶活性初步研究。经同源序列比对分析,思茅松毛虫4龄幼虫肠道中共分离得到12株真菌,分别属于德巴利酵母属Debaryomyces sp.,拟盘多毛孢属Pestalotiopsis sp.,青霉属Penicillium sp.,弯担菌属Curvibasidium sp.。产酶活性研究表明8株菌产纤维素酶,9株菌产淀粉酶,7株菌产脂肪酶,2株菌产蛋白酶。DKF-8产淀粉酶能力最高,酶活力是60.907 U/mL。DKF-10产纤维素酶能力最高,酶活力是14.276 U/g,菌株DKF-6产蛋白酶活力是5.561 U/mL,菌株DKF-8产蛋白酶酶活力是2.918 U/mL。思茅松毛虫4龄幼虫肠道真菌物种丰富度较低。本实验为未来深入研究思茅松毛虫肠道微生物功能提供了菌株材料。
Intestinal microorganisms play an important role in insect food digestion and immune defense, but little is known about intestinal fungi. In this study, Dendrolimu kikuchii Matsumura, an important forestry pest was used to isolate and identify intestinal fungi from its larvae. The intestinal fungi of the 4 instar larvae of D. kikuchii were isolated by pure culture and identified by ITS sequence analysis. Then their enzyme activity was preliminarily studied. After homologous sequence alignment analysis, 12 strains of fungi were isolated from the intestinal tract of the 4 instar larvae of D. kikuchii. The isloated fungi were Debaryomyces sp., Pestalotiopsis sp., Penicillium sp. and Curvibasidium sp. Among them, results showed that 8 strains produced cellulase, 9 strains produced amylase, 7 strains produced lipase, and 2 strains produced protease. DKF-8 ha the highest amylase producing ability and the enzyme activity was 60.907 U/mL. The cellulase activity of DKF-10 was 14.276 U/g, The protease activity of DKF-6 was 5.561 U/mL and DKF-8 was 2.918 U/mL. The species diversity of intestinal fungi of 4 instar larvae of D. kikuchii was relatively deficient. This work provides potential available strains for functional studies of the gut fungi in D. kikuchii.
Intestinal microorganisms play an important role in insect food digestion and immune defense, but little is known about intestinal fungi. In this study, Dendrolimu kikuchii Matsumura, an important forestry pest was used to isolate and identify intestinal fungi from its larvae. The intestinal fungi of the 4 instar larvae of D. kikuchii were isolated by pure culture and identified by ITS sequence analysis. Then their enzyme activity was preliminarily studied. After homologous sequence alignment analysis, 12 strains of fungi were isolated from the intestinal tract of the 4 instar larvae of D. kikuchii. The isloated fungi were Debaryomyces sp., Pestalotiopsis sp., Penicillium sp. and Curvibasidium sp. Among them, results showed that 8 strains produced cellulase, 9 strains produced amylase, 7 strains produced lipase, and 2 strains produced protease. DKF-8 ha the highest amylase producing ability and the enzyme activity was 60.907 U/mL. The cellulase activity of DKF-10 was 14.276 U/g, The protease activity of DKF-6 was 5.561 U/mL and DKF-8 was 2.918 U/mL. The species diversity of intestinal fungi of 4 instar larvae of D. kikuchii was relatively deficient. This work provides potential available strains for functional studies of the gut fungi in D. kikuchii.
引文
Cai WC, Li MY, Li Y, et al. Application of Dendrolimu kikuchii CPV against Dendrolimu kikuchii mats [J]. Forest Pest and Disease, 2004, 2: 21-23. [蔡文翠, 黎茂尧, 黎勇, 等. 思茅松毛虫质型多角体病毒防治思茅松毛虫研究 [J]. 中国森林病虫, 2004, 2: 21-23]
Chen BS, Lu XM, Shao YQ. Diversity of the gut microbiota in lepidopteran insects and their interaction with hosts [J]. Acta Entomologica Sinica, 2017, 60 (6): 710-722. [陈勃生, 鲁兴萌, 邵勇奇. 鳞翅目昆虫肠道微生物的多样性及其与宿主的相互作用 [J]. 昆虫学报, 2017, 60 (6): 710-722]
Chen J, Kuang CB. Isolation, identification of a cellulose-degradation fungus [J]. Journal of Southwest University (Natural Science Edition), 2016, 38 (8): 22-26. [陈静, 匡成兵. 纤维素降解真菌的分离筛选 [J]. 西南大学学报 (自然科学版), 2016, 38 (8): 22-26]
Chen JZ, Tu X, Cheng F, et al. Isolation, identification and antibacterial activity of intestinal symbiotic fungi from Acrida cinerea [J]. Journal of China Three Gorges University (Natural Sciences), 2014, 36 (3): 108-112. [陈君芝, 涂璇, 程凡, 等. 中华剑角蝗肠道共生真菌的分离鉴定及抑菌活性筛选 [J]. 三峡大学学报(自然科学版), 2014, 36 (3): 108-112]
Deng SC. Analysis of the effect of Beauveria bassiana on the control of Dendrolimu kikuchii [J]. Yunnan Forest Inventory and Planning, 1992, 4: 19-20. [邓书采. 白僵菌防治思茅松毛虫的效果分析 [J]. 云南林业调查规划, 1992, 4: 19-20]
Deng XM. Prelimary study on using compound biological insecticides to control Dendrolimu kikuchii [J]. Journal of Fujian Forestry Science and Technology, 2002, 3: 33-36. [邓秀明. 复合生物杀虫剂防治思茅松毛虫的初步研究 [J]. 福建林业科技, 2002, 3: 33-36]
Gilliam M. Identification and roles of non-pathogenic Microflora associated with honey bees [J]. Fems Microbiology Letters, 1997, 155: 1-10.
Guo QL. Investigation of Diversity and Functional Enzymes of Fungi from Erdos a Paleo Salt Lake in Inner Mongolia [D].Hanzhong: Shaanxi University of Technology, 2014. [郭庆兰. 内蒙古鄂尔多斯一古盐湖真菌多样性及其功能酶的筛选 [D]. 汉中:陕西理工学院, 2014]
Hou TQ. The Pine Caterpillars in China [M]. Beijing: Science Press, 1987: 32-34. [侯陶谦. 中国松毛虫 [M]. 北京: 科学出版社, 1987: 32-34]
Hu X, Li M, Chen H. Community structure of gut fungi during different developmental stages of the Chinese white pine beetle [J]. Scientific Reports, 2015, 5 (5315): 8411.
Jiang XZ, Yang EC, Liu XZ. Relationship and coevolution between fungi and insects [J]. Frontier Science, 2009, 3 (1): 12-21. [蒋先芝, 杨恩策, 刘杏忠. 真菌和昆虫的互作关系及协同进化 [J]. 前沿科学, 2009, 3 (1): 12-21]
Kang L, Wang JH, Sun YH, et al. Diversity analysis of intestinal aerobic bacteria isolated from 3th instar Dendrolimu kikuchii larvae by ARDRA [J]. Hubei Agricultural Sciences, 2012, 51 (7): 1481-1483. [康柳, 王金华, 孙佑赫, 等. 3龄思茅松毛虫幼虫肠道好氧细菌的分离及ARDRA多态性分析 [J]. 湖北农业科学, 2012, 51 (7): 1481-1483]
Li YL, Fan LY, Fan YY, et al. Screening, identification of endophytic fungus produing raw starch-degrading enzyme from Salvia miltiorrhiza Bge.and its enzymatic properties [J]. Science and Technology of Food Industry , 2017, 38 (9): 108-112. [李艳玲, 樊鲁玉, 侯衍英, 等. 丹参内生真菌产生淀粉酶菌株的筛选鉴定及酶学性质分析 [J]. 食品工业科技, 2017, 38 (9): 108-112]
Liu SX, Wang JH, Xiong Z, et al. Inhibitory effect of ethanol extract from Artemisia annua on intestinal bacteria of 5th instar larva of Dendrolimu kikuchii [J]. Jiangsu Agricultural Sciences, 2012, 40 (4): 141-142. [刘绍雄, 王金华, 熊智, 等. 青蒿乙醇提取物对5龄思茅松毛虫肠道细菌的抑制作用 [J]. 江苏农业科学, 2012, 40 (4): 141-142]
Liu SX, Wang MY, Wang JH, et al. Inhibition effect of Castanea mollissima Blume bract extract on intestinal bacteria in Dendrolimu kikuchii Matsumura [J]. Journal of Southern Agriculture, 2012, 43 (12): 1968-1972. [刘绍雄, 王明月, 王金华, 等. 板栗苞壳提取物对思茅松毛虫肠道细菌的抑菌作用 [J]. 南方农业学报, 2012, 43 (12): 1968-1972]
Ma YF, Chen SF, Wang JH, et al. Screening and virulence of intestinal aerobic bacteria from the 7th instar larvae of Dendrolimu kikuchii [J]. Forest Pest and Disease, 2012, 31 (1): 1-4. [马艳芳, 陈升富, 王金华, 等. 思茅松毛虫7龄幼虫肠道好氧细菌的筛选及毒力测定 [J]. 中国森林病虫, 2012, 31 (1): 1-4]
Qiao H, Han TY, Zhang QF. Research on the enzyme-producing capacity of marine fungi [J]. China Brewing, 2018, 37 (1): 59-62. [乔慧, 韩廷玉, 张庆芳. 海洋真菌产酶能力的研究 [J]. 中国酿造, 2018, 37 (1): 59-62]
Schloss PD, Delalibera I, Handelsman J, et al. Bacteria associated with the guts of two wood-boring beetles: Anoplophora glabripennis and Saperda vestita (Cerambycidae) [J]. Environmental Entomolgy, 2006, 35 (3): 625-629.
Shi L, Zhao Y, Zhang ZH, et al. Effect of compound microbial insecticide on pine caterpillar control in large area [J]. Forest Research, 2004, 4: 461-464. [石雷, 赵耘, 张忠和, 等. 复合微生物杀虫剂大面积防治松毛虫的效果 [J]. 林业科学研究, 2004, 4: 461-464]
Shao MW.Indentification and Metabolites of Symbiotic Fungi Isolated the Gut of Two Kinds of Dragonflies [D]. Jinhua: Zhejing Normal University, 2015. [邵明伟. 两种蜻蜓肠道菌的分离鉴定及其代谢产物研究 [D]. 金华:浙江师范大学, 2015]
Sun YH, Xiong Z, Wang JH, et al. Amplified ribosomal DNA restriction analysis and identification of intestinal aerobic bacteria from 4th instar larva of Dendrolimu kikuchii [J]. Chinese Journal of Applied Entomology, 2012, 49 (6): 1618-1622. [孙佑赫, 熊智, 王金华, 等. 思茅松毛虫四龄幼虫肠道好氧细菌的ARDRA分析及鉴定 [J]. 应用昆虫学报, 2012, 49 (6): 1618-1622]
Sun YH, Xiong Z, Wang JH, et al. ARDRA and identification of intestinal bacteria from 6th instar larvae of Dendrolimu kikuchii [J]. Hubei Agricultural Sciences, 2012, 51 (8): 1684-1686. [孙佑赫, 熊智, 王金华, 等. 思茅松毛虫6龄幼虫肠道细菌的ARDRA分析与鉴定 [J]. 湖北农业科学, 2012, 51 (8): 1684-1686]
Sun XX. Studies on the Gut Microbial Diversity and Isolation, Identification of Chitin-Degrading Bacteria from the Hindgut of Macrotermes barneyi [D]. Jinan: Shandong University, 2017. [孙新新. 黄翅大白蚁肠道微生物多样性及几丁质降解微生物的分离与鉴定 [D]. 济南: 山东大学, 2017]
Sunitha VH, Devi DN, Srinivas C. Extracellular enzymatic activity of endophytic fungal strains isolated from medicinal plants [J]. World Journal of Agricultural Sciences , 2013, 9 (1): 1-9.
Tong Q, Liu HP. Research advances of Dendrolimu kikuchii [J]. Forest Pest and Disease, 2009, 28 (4): 23-26. [童清, 刘宏屏. 思茅松毛虫研究进展 [J]. 中国森林病虫, 2009, 28 (4): 23-26]
Xiang H, Huang YP. Symbiosis between gut microbiota and insects [J]. Chinese Bulletin of Entomology, 2008, 5: 687-693. [相辉, 黄勇平. 肠道微生物与昆虫的共生关系 [J]. 昆虫知识, 2008, 5: 687-693]
Yang MM.Identity and Genomic Sequence Analysis of Dendrolimu kikuchii Nucleopolyhendrovirus [D]. Yangling:Northwest A & F University, 2012. [杨苗苗. 思茅松毛虫核型多角体病毒及全基因组分析 [D]. 杨陵:西北农林科技大学, 2012]
Yu HW, Wang ZK, Liu L, et al. Analysis of fungal diversity in intestines of Hepialus gonggaensis larvae [J]. Acta Microbiologica Sinica, 2008, 4: 439-445. [俞和韦, 王中康, 刘莉, 等. 贡嘎蝠蛾幼虫肠道真菌多样性分析 [J]. 微生物学报, 2008, 4: 439-445]
Zeng JP, Ge F, Su JW, et al. Researches on the occurrences of major forest insect pests of pine caterpillar Dendrolimus sp. in China [J]. Chinese Bullletin of Entomology, 2010, 47 (3): 451-459.[曾菊平, 戈峰, 苏建伟, 等. 我国林业重大害虫松毛虫的灾害研究进展 [J]. 昆虫知识, 2010, 47 (3): 451-459]
Zeng SS, Yang G, Yang ZX, et al. Application of Dendrolimu kikuchii cytoplasmic polyhedrosis virus [J]. Forest Pest and Disease, 2000, 19 (1): 28-29. [曾述圣, 杨桂, 杨中学, 等. 思茅松毛虫质型多角体病毒的应用 [J]. 中国森林病虫, 2000, 19 (1): 28-29]
Zhang WX, Wang JH, Xiong Z, et al. Screening and toxicity determination of intestinal aerobic bacteria from 2nd instar larva of Dendrolimu kikuchii [J]. Journal of Anhui Agricultural Sciences, 2011, 39 (28): 17288-17290. [张武先, 王金华, 熊智, 等. 思茅松毛虫2龄幼虫肠道好氧细菌的筛选及毒力测定 [J]. 安徽农业科学, 2011, 39 (28): 17288-17290]
Zhang ZY, Sheng P, Huang SW, et al. Diversity, function and application of insect gut microbiota [J]. Biotic Resources, 2017, 39 (4): 231-239. [张振宇, 圣平, 黄胜威. 昆虫肠道微生物的多样性、功能及应用 [J]. 生物资源, 2017, 39 (4): 231-239]
Zhang Y. Investigation On Bioactive Metabolites Producted by Three Insect Gut Fungi [D]. Jinhua: Zhejing Normal University, 2015. [张蕴. 三株昆虫肠道共生菌的活性代谢产物研究 [D]. 金华:浙江师范大学, 2015]
Zhou DQ, Xu DQ. Microbiology Experiment Course [M]. Beijing: Higher Education Press, 2013: 350-353. [周德庆, 徐德强. 微生物学实验教程 [M]. 北京: 高等教育出版社, 2013: 350-353]
Zhu LL, Liu SX, Shi R, et al. Inhibition effect of Ebenaceae Diospyros kaki leaf extract on intestinal bacteria from instar larvae of Dendrolimus kikuchii Matsumura [J]. Science and Technology of Food Industry, 2013, 34 (7): 123-125. [朱丽丽, 刘邵雄, 施蕊, 等.柿子叶提取物对思茅松毛虫肠道细菌抑制作用的研究 [J]. 食品工业科技, 2013, 34 (7): 123-125]
Zhu LL, Liu SX, Wang JH, et al. Inhibition effects of ethanol extracts from Eupatorium adenophorum spreng on intestinal bacteria from 5th instar larvae of Dendrolimu kikuchii [J]. Journal of Henan Agricultural Sciences, 2012, 41 (3): 98-100. [朱丽丽, 刘邵雄, 王金华, 等. 紫茎泽兰乙醇提取物对五龄思茅松毛虫肠道细菌的抑制作用 [J]. 河南农业科学, 2012, 41 (3): 98-100]
Zhu XF. Modern Experimental Technique of Microbiology [M]. Hangzhou: Zhejiang University Press, 2011: 269-275. [朱旭芬. 现代微生物学实验技术 [M]. 杭州: 浙江大学出版社, 2011: 269-275]
Chen BS, Lu XM, Shao YQ. Diversity of the gut microbiota in lepidopteran insects and their interaction with hosts [J]. Acta Entomologica Sinica, 2017, 60 (6): 710-722. [陈勃生, 鲁兴萌, 邵勇奇. 鳞翅目昆虫肠道微生物的多样性及其与宿主的相互作用 [J]. 昆虫学报, 2017, 60 (6): 710-722]
Chen J, Kuang CB. Isolation, identification of a cellulose-degradation fungus [J]. Journal of Southwest University (Natural Science Edition), 2016, 38 (8): 22-26. [陈静, 匡成兵. 纤维素降解真菌的分离筛选 [J]. 西南大学学报 (自然科学版), 2016, 38 (8): 22-26]
Chen JZ, Tu X, Cheng F, et al. Isolation, identification and antibacterial activity of intestinal symbiotic fungi from Acrida cinerea [J]. Journal of China Three Gorges University (Natural Sciences), 2014, 36 (3): 108-112. [陈君芝, 涂璇, 程凡, 等. 中华剑角蝗肠道共生真菌的分离鉴定及抑菌活性筛选 [J]. 三峡大学学报(自然科学版), 2014, 36 (3): 108-112]
Deng SC. Analysis of the effect of Beauveria bassiana on the control of Dendrolimu kikuchii [J]. Yunnan Forest Inventory and Planning, 1992, 4: 19-20. [邓书采. 白僵菌防治思茅松毛虫的效果分析 [J]. 云南林业调查规划, 1992, 4: 19-20]
Deng XM. Prelimary study on using compound biological insecticides to control Dendrolimu kikuchii [J]. Journal of Fujian Forestry Science and Technology, 2002, 3: 33-36. [邓秀明. 复合生物杀虫剂防治思茅松毛虫的初步研究 [J]. 福建林业科技, 2002, 3: 33-36]
Gilliam M. Identification and roles of non-pathogenic Microflora associated with honey bees [J]. Fems Microbiology Letters, 1997, 155: 1-10.
Guo QL. Investigation of Diversity and Functional Enzymes of Fungi from Erdos a Paleo Salt Lake in Inner Mongolia [D].Hanzhong: Shaanxi University of Technology, 2014. [郭庆兰. 内蒙古鄂尔多斯一古盐湖真菌多样性及其功能酶的筛选 [D]. 汉中:陕西理工学院, 2014]
Hou TQ. The Pine Caterpillars in China [M]. Beijing: Science Press, 1987: 32-34. [侯陶谦. 中国松毛虫 [M]. 北京: 科学出版社, 1987: 32-34]
Hu X, Li M, Chen H. Community structure of gut fungi during different developmental stages of the Chinese white pine beetle [J]. Scientific Reports, 2015, 5 (5315): 8411.
Jiang XZ, Yang EC, Liu XZ. Relationship and coevolution between fungi and insects [J]. Frontier Science, 2009, 3 (1): 12-21. [蒋先芝, 杨恩策, 刘杏忠. 真菌和昆虫的互作关系及协同进化 [J]. 前沿科学, 2009, 3 (1): 12-21]
Kang L, Wang JH, Sun YH, et al. Diversity analysis of intestinal aerobic bacteria isolated from 3th instar Dendrolimu kikuchii larvae by ARDRA [J]. Hubei Agricultural Sciences, 2012, 51 (7): 1481-1483. [康柳, 王金华, 孙佑赫, 等. 3龄思茅松毛虫幼虫肠道好氧细菌的分离及ARDRA多态性分析 [J]. 湖北农业科学, 2012, 51 (7): 1481-1483]
Li YL, Fan LY, Fan YY, et al. Screening, identification of endophytic fungus produing raw starch-degrading enzyme from Salvia miltiorrhiza Bge.and its enzymatic properties [J]. Science and Technology of Food Industry , 2017, 38 (9): 108-112. [李艳玲, 樊鲁玉, 侯衍英, 等. 丹参内生真菌产生淀粉酶菌株的筛选鉴定及酶学性质分析 [J]. 食品工业科技, 2017, 38 (9): 108-112]
Liu SX, Wang JH, Xiong Z, et al. Inhibitory effect of ethanol extract from Artemisia annua on intestinal bacteria of 5th instar larva of Dendrolimu kikuchii [J]. Jiangsu Agricultural Sciences, 2012, 40 (4): 141-142. [刘绍雄, 王金华, 熊智, 等. 青蒿乙醇提取物对5龄思茅松毛虫肠道细菌的抑制作用 [J]. 江苏农业科学, 2012, 40 (4): 141-142]
Liu SX, Wang MY, Wang JH, et al. Inhibition effect of Castanea mollissima Blume bract extract on intestinal bacteria in Dendrolimu kikuchii Matsumura [J]. Journal of Southern Agriculture, 2012, 43 (12): 1968-1972. [刘绍雄, 王明月, 王金华, 等. 板栗苞壳提取物对思茅松毛虫肠道细菌的抑菌作用 [J]. 南方农业学报, 2012, 43 (12): 1968-1972]
Ma YF, Chen SF, Wang JH, et al. Screening and virulence of intestinal aerobic bacteria from the 7th instar larvae of Dendrolimu kikuchii [J]. Forest Pest and Disease, 2012, 31 (1): 1-4. [马艳芳, 陈升富, 王金华, 等. 思茅松毛虫7龄幼虫肠道好氧细菌的筛选及毒力测定 [J]. 中国森林病虫, 2012, 31 (1): 1-4]
Qiao H, Han TY, Zhang QF. Research on the enzyme-producing capacity of marine fungi [J]. China Brewing, 2018, 37 (1): 59-62. [乔慧, 韩廷玉, 张庆芳. 海洋真菌产酶能力的研究 [J]. 中国酿造, 2018, 37 (1): 59-62]
Schloss PD, Delalibera I, Handelsman J, et al. Bacteria associated with the guts of two wood-boring beetles: Anoplophora glabripennis and Saperda vestita (Cerambycidae) [J]. Environmental Entomolgy, 2006, 35 (3): 625-629.
Shi L, Zhao Y, Zhang ZH, et al. Effect of compound microbial insecticide on pine caterpillar control in large area [J]. Forest Research, 2004, 4: 461-464. [石雷, 赵耘, 张忠和, 等. 复合微生物杀虫剂大面积防治松毛虫的效果 [J]. 林业科学研究, 2004, 4: 461-464]
Shao MW.Indentification and Metabolites of Symbiotic Fungi Isolated the Gut of Two Kinds of Dragonflies [D]. Jinhua: Zhejing Normal University, 2015. [邵明伟. 两种蜻蜓肠道菌的分离鉴定及其代谢产物研究 [D]. 金华:浙江师范大学, 2015]
Sun YH, Xiong Z, Wang JH, et al. Amplified ribosomal DNA restriction analysis and identification of intestinal aerobic bacteria from 4th instar larva of Dendrolimu kikuchii [J]. Chinese Journal of Applied Entomology, 2012, 49 (6): 1618-1622. [孙佑赫, 熊智, 王金华, 等. 思茅松毛虫四龄幼虫肠道好氧细菌的ARDRA分析及鉴定 [J]. 应用昆虫学报, 2012, 49 (6): 1618-1622]
Sun YH, Xiong Z, Wang JH, et al. ARDRA and identification of intestinal bacteria from 6th instar larvae of Dendrolimu kikuchii [J]. Hubei Agricultural Sciences, 2012, 51 (8): 1684-1686. [孙佑赫, 熊智, 王金华, 等. 思茅松毛虫6龄幼虫肠道细菌的ARDRA分析与鉴定 [J]. 湖北农业科学, 2012, 51 (8): 1684-1686]
Sun XX. Studies on the Gut Microbial Diversity and Isolation, Identification of Chitin-Degrading Bacteria from the Hindgut of Macrotermes barneyi [D]. Jinan: Shandong University, 2017. [孙新新. 黄翅大白蚁肠道微生物多样性及几丁质降解微生物的分离与鉴定 [D]. 济南: 山东大学, 2017]
Sunitha VH, Devi DN, Srinivas C. Extracellular enzymatic activity of endophytic fungal strains isolated from medicinal plants [J]. World Journal of Agricultural Sciences , 2013, 9 (1): 1-9.
Tong Q, Liu HP. Research advances of Dendrolimu kikuchii [J]. Forest Pest and Disease, 2009, 28 (4): 23-26. [童清, 刘宏屏. 思茅松毛虫研究进展 [J]. 中国森林病虫, 2009, 28 (4): 23-26]
Xiang H, Huang YP. Symbiosis between gut microbiota and insects [J]. Chinese Bulletin of Entomology, 2008, 5: 687-693. [相辉, 黄勇平. 肠道微生物与昆虫的共生关系 [J]. 昆虫知识, 2008, 5: 687-693]
Yang MM.Identity and Genomic Sequence Analysis of Dendrolimu kikuchii Nucleopolyhendrovirus [D]. Yangling:Northwest A & F University, 2012. [杨苗苗. 思茅松毛虫核型多角体病毒及全基因组分析 [D]. 杨陵:西北农林科技大学, 2012]
Yu HW, Wang ZK, Liu L, et al. Analysis of fungal diversity in intestines of Hepialus gonggaensis larvae [J]. Acta Microbiologica Sinica, 2008, 4: 439-445. [俞和韦, 王中康, 刘莉, 等. 贡嘎蝠蛾幼虫肠道真菌多样性分析 [J]. 微生物学报, 2008, 4: 439-445]
Zeng JP, Ge F, Su JW, et al. Researches on the occurrences of major forest insect pests of pine caterpillar Dendrolimus sp. in China [J]. Chinese Bullletin of Entomology, 2010, 47 (3): 451-459.[曾菊平, 戈峰, 苏建伟, 等. 我国林业重大害虫松毛虫的灾害研究进展 [J]. 昆虫知识, 2010, 47 (3): 451-459]
Zeng SS, Yang G, Yang ZX, et al. Application of Dendrolimu kikuchii cytoplasmic polyhedrosis virus [J]. Forest Pest and Disease, 2000, 19 (1): 28-29. [曾述圣, 杨桂, 杨中学, 等. 思茅松毛虫质型多角体病毒的应用 [J]. 中国森林病虫, 2000, 19 (1): 28-29]
Zhang WX, Wang JH, Xiong Z, et al. Screening and toxicity determination of intestinal aerobic bacteria from 2nd instar larva of Dendrolimu kikuchii [J]. Journal of Anhui Agricultural Sciences, 2011, 39 (28): 17288-17290. [张武先, 王金华, 熊智, 等. 思茅松毛虫2龄幼虫肠道好氧细菌的筛选及毒力测定 [J]. 安徽农业科学, 2011, 39 (28): 17288-17290]
Zhang ZY, Sheng P, Huang SW, et al. Diversity, function and application of insect gut microbiota [J]. Biotic Resources, 2017, 39 (4): 231-239. [张振宇, 圣平, 黄胜威. 昆虫肠道微生物的多样性、功能及应用 [J]. 生物资源, 2017, 39 (4): 231-239]
Zhang Y. Investigation On Bioactive Metabolites Producted by Three Insect Gut Fungi [D]. Jinhua: Zhejing Normal University, 2015. [张蕴. 三株昆虫肠道共生菌的活性代谢产物研究 [D]. 金华:浙江师范大学, 2015]
Zhou DQ, Xu DQ. Microbiology Experiment Course [M]. Beijing: Higher Education Press, 2013: 350-353. [周德庆, 徐德强. 微生物学实验教程 [M]. 北京: 高等教育出版社, 2013: 350-353]
Zhu LL, Liu SX, Shi R, et al. Inhibition effect of Ebenaceae Diospyros kaki leaf extract on intestinal bacteria from instar larvae of Dendrolimus kikuchii Matsumura [J]. Science and Technology of Food Industry, 2013, 34 (7): 123-125. [朱丽丽, 刘邵雄, 施蕊, 等.柿子叶提取物对思茅松毛虫肠道细菌抑制作用的研究 [J]. 食品工业科技, 2013, 34 (7): 123-125]
Zhu LL, Liu SX, Wang JH, et al. Inhibition effects of ethanol extracts from Eupatorium adenophorum spreng on intestinal bacteria from 5th instar larvae of Dendrolimu kikuchii [J]. Journal of Henan Agricultural Sciences, 2012, 41 (3): 98-100. [朱丽丽, 刘邵雄, 王金华, 等. 紫茎泽兰乙醇提取物对五龄思茅松毛虫肠道细菌的抑制作用 [J]. 河南农业科学, 2012, 41 (3): 98-100]
Zhu XF. Modern Experimental Technique of Microbiology [M]. Hangzhou: Zhejiang University Press, 2011: 269-275. [朱旭芬. 现代微生物学实验技术 [M]. 杭州: 浙江大学出版社, 2011: 269-275]