斜纹夜蛾3种SlitPBPs的组织表达模式分析
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摘要
性信息素结合蛋白(pheromone binding proteins,PBPs)能够与性信息素分子结合,从而启动昆虫的寻偶及交配行为。本研究采用半定量RT-PCR技术分别对斜纹夜蛾3种性信息素结合蛋白SlitPBP1、SlitPBP2和SlitPBP3基因在雌、雄虫不同组织的基因表达模式进行了比较分析。组织表达模式结果表明,SlitPBP1基因只在雌、雄虫触角和雌虫前足中表达而在其他组织中无表达;SlitPBP2基因在雌虫的触角、喙、前足、中足、后足、翅膀和雄虫的触角、喙、前足、翅膀中均有表达,且在同一部位,SlitPBP2基因在雌虫的表达量均高于雄虫相应部位的表达量;除雌、雄虫的胸部外,SlitPBP3在雌、雄虫的触角、喙、前足、中足、后足、腹部和翅膀中均有较高水平的表达。可见,SlitPBP1、SlitPBP2和SlitPBP3基因在斜纹夜蛾不同组织的表达模式各不相同。本研究结果可为深入研究不同SlitPBPs蛋白在斜纹夜蛾生长发育过程中的不同生理功能提供科学参考。
The pheromone binding proteins(PBPs) can specifically bind to sex pheromone molecules, thereby initiating insect mate behavior. The semi-quantitative RT-PCR technique was used to analyze the tissues expression patterns of SlitPBP1, SlitPBP2 and SlitPBP3 in Spodoptera litura. The results of tissue expression patterns indicate that the SlitPBP1 is expressed only in the antennae of the male and female S.litura and the female forefoot. But, SlitPBP1 is not expressed in other tissues. The SlitPBP2 is expressed in the female antennae, proboscis, forefoot, middle foot, hind foot, wings and male antennae, proboscis, forefoot and wings. For the same tissue, the expression level of SlitPBP2 in females is higher than that of males. In addition to the thorax of male and female S. litura, SlitPBP3 has higher expression levels in the antennae, proboscis, forefoot, middle foot, hind foot, abdomen and wings. It can be seen that the expression patterns of SlitPBP1, SlitPBP2 and SlitPBP3 in different tissues of S. litura are different. The results of this study can provide a scientific reference for further study of different physiological functions of different SlitPBPs in the development of S. litura.
The pheromone binding proteins(PBPs) can specifically bind to sex pheromone molecules, thereby initiating insect mate behavior. The semi-quantitative RT-PCR technique was used to analyze the tissues expression patterns of SlitPBP1, SlitPBP2 and SlitPBP3 in Spodoptera litura. The results of tissue expression patterns indicate that the SlitPBP1 is expressed only in the antennae of the male and female S.litura and the female forefoot. But, SlitPBP1 is not expressed in other tissues. The SlitPBP2 is expressed in the female antennae, proboscis, forefoot, middle foot, hind foot, wings and male antennae, proboscis, forefoot and wings. For the same tissue, the expression level of SlitPBP2 in females is higher than that of males. In addition to the thorax of male and female S. litura, SlitPBP3 has higher expression levels in the antennae, proboscis, forefoot, middle foot, hind foot, abdomen and wings. It can be seen that the expression patterns of SlitPBP1, SlitPBP2 and SlitPBP3 in different tissues of S. litura are different. The results of this study can provide a scientific reference for further study of different physiological functions of different SlitPBPs in the development of S. litura.
引文
Armes NJ, Wightman JA, Jadhav DR, et al. Status of insecticide resistance in Spodoptera litura in Andhra pradesh, India [J]. Pest Management Science, 2015, 50 (3): 240-248.
Callahan FE, Vogt RG, Tucker ML, et al. High level expression of "male specific" pheromone binding proteins (PBPs) in the antennae of female noctuiid moths [J]. Insect Biochemistry and Molecular Biology, 2000, 30 (6): 507-514.
Dong K.Structure Characterization and Functional Differentiation of Pheromone Binding Proteins in Cotton Worm Helicoverpa armigera [D]. Beijing: China Agricultural University, 2017. [董昆. 棉铃虫性信息素结合蛋白结构特征及功能分化研究 [D]. 北京:中国农业大学, 2017]
Gong DP, Zhao P, Lin Y, et al. Identification and expression pattern of two pheromone binding proteins in silkworm, Bombyx mori [J]. Acta Entomology Sinica, 2006, 49 (3): 355-362. [龚达平, 赵萍, 林英, 等. 家蚕信息素结合蛋白BmPBP2和BmPBP3基因的初步鉴定及表达分析 [J]. 昆虫学报, 2006, 49 (3): 355-362]
Gu SH. Sex pheromone recognition and immunolocalization of three pheromone binding proteins in the black cutworm moth Agrotis ipsilon [J]. Insect Biochemistry and Molecular Biology, 2013, 43 (3): 237-251.
Gong ZJ, Zhou WW, Yu HZ, et al. Cloning, expression and functional analysis of a general odorant-binding protein 2 gene of the rice striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) [J]. Insect Molecular Biology, 2009, 18 (3): 405-417.
He YM. Green control and integration technology of Spodoptera litura [J]. Scientific Breeding, 2017, 11: 34-35. [何永梅. 斜纹夜蛾绿色防控集成技术 [J]. 科学种养, 2017, 11: 34-35]
Jia XJ, Hao SD, Du YL, et al. cDNA cloning, expression profiling and binding affinity assay of the pheromone binding protein Cpun-PBP1 in the yellow peach moth, Conogethes punctiferalis (Lepidoptera:Crambidae) [J]. Acta Entomology Sinica, 2015, 58 (11): 1167-1176. [贾小俭, 郝少东, 杜艳丽, 等. 桃蛀螟性信息素结合蛋白Cpun-PBP1的cDNA克隆、表达谱及其与配体化合物的结合特性分析 [J]. 昆虫学报, 2015, 58 (11): 1167-1176]
Laue M, Steinbrecht RA. Topochemistry of moth olfactory sensilla [J]. International Journal of Insect Morphology & Embryology, 1997, 26 (3): 217-228.
Leal WS. Odorant reception in insects: Roles of receptors, binding proteins, and degrading enzymes [J]. Annual Review of Entomology, 2013, 58: 373-391.
Li L, Yang WL, Guo XR, et al. Cloning sequence analysis and spatio-temporal expression of a pheromone binding protein 2 (PBP2) gene from Helicoverpa assulta (Guenée)(Lepidoptera: Noctuidae) [J]. Acta Entomology Sinica, 2009, 52 (11): 1199-1205. [李亮, 杨文玲, 郭线茹, 等. 烟夜蛾性信息素结合蛋白2(PBP2)基因的克隆、序列分析与时空表达 [J]. 昆虫学报, 2009, 52 (11): 1199-1205]
Li XT, Luo HY, Chen YW, et al. Control efficiency of different biological control technologies on three major pest insects of tobacco [J]. Crop Research, 2011, 25 (4): 361-365. [李晓婷, 罗华元, 陈月舞, 等. 不同生物防治技术对烟草烟蚜和烟青虫及斜纹夜蛾的防治效果 [J]. 作物研究, 2011, 25 (4): 361-365]
Liu NY. FunctionalDifferentiation of Pheromone Binding Protein /General Odorant-bingding Protein in Spodoptera exigua and Spodoptera litura [D]. Nanjing: Nanjing Agricultural University, 2015. [刘乃勇. 甜菜夜蛾和斜纹夜蛾PBP/GOBP的功能分化研究 [D]. 南京:南京农业大学, 2015]
Liu NY, Liu CC, Dong SL. Functional differentiation of pheromone-binding proteins in the common cutworm Spodoptera litura [J]. Comparative Biochemistry & Physiology Part A Molecular & Integrative Physiology, 2013, 165 (2): 254-262.
Miu S, Zhang L, Wang LH. Study progress of insect odorant-binding protein [J]. Journal of Yunnan University, 2003, 25: 161-165. [缪森, 张龙, 王丽红. 昆虫气味结合蛋白研究进展 [J]. 云南大学学报, 2003, 25: 161-165]
Mohanty S, Zubkov S, Gronenborn AM. The solution NMR structure of Antheraea polyphemus PBP provides new in-sight into pheromone recognition by pheromone-binding proteins [J]. Journal of Molecular Biology, 2004, 337 (2): 443-451.
Mukunda L, Miazzi F, Kaltofen S, et al. Calmodulin modulates insect odorant receptor function [J]. Cell Calcium, 2014, 55 (4): 191-199.
Nie NS, Huang SJ, Long QL. Insecticide-resistance and control strategy for common cutworm, Spodoptera litura (Fab.) [J]. Acta Agriculturae Jiangxi, 2007, 19 (7): 51-54. [聂南生, 黄水金, 龙丘陵. 斜纹夜蛾的抗药性及其治理策略 [J]. 江西农业学报, 2007, 19 (7): 51-54]
Pelosi P, Maida R. Odorant-binding proteins in insects [J]. Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology, 1995, 111 (3): 503-514.
Qian HY, Xu AY. Advances in research on insect pheromone binding proteins [J]. Anhui Agricultural Science Bulletin, 2007, 23: 128-130. [钱荷英, 徐安英. 昆虫信息素结合蛋白研究进展 [J]. 安徽农学通报, 2007, 23: 128-130]
Sang S, Wang Z, Qi JW, et al. Research progresses on pesticide resistance of Spodoptera litura [J]. Journal of Environmmental Entomolgy, 2013, 35 (6): 808-814. [桑松, 王政, 齐江卫, 等. 斜纹夜蛾抗药性研究进展 [J]. 环境昆虫学报, 2013, 35 (6): 808-814]
Sun M, Liu Y, Wang G. Expression patterns and binding properties of three pheromone binding proteins in the diamondback moth, Plutella xyllotella [J]. Journal of Insect Physiology, 2012, 59 (1): 46-55.
Tian Z, Zhang Y. Molecular characterization and functional analysis of pheromone binding protein 1 from Cydia pomonella (L.) [J]. Insect Molecular Biology, 2016, 25 (6): 769-777.
Tian ZQ, Sun LN, Li YY, et al. Research progress of the function of sex pheromone binding protein in insects [J]. Journal of Agriculture, 2017, 7 (9): 14-20. [田志强, 孙丽娜, 李艳艳, 等. 昆虫性信息素结合蛋白功能研究进展 [J]. 农学学报, 2017, 7 (9): 14-20]
Vogt RG, Riddiford LM. Pheromone binding and inactivation by moth antennae [J]. Nature, 1981, 293: 161-163.
Wang JZ, Hu P, Luo YQ, et al. Identification and expression patterns of the pheromone binding protein genes AglaPBP1 and AglaPBP2 in Anoplophora glabripennis (Coleoptera:Cerambycidae) [J]. Chinese Journal of Applied Entomology, 2017, 54 (1): 45-55. [王菁桢, 胡平, 骆有庆, 等. 光肩星天牛性信息素结合蛋白AglaPBP1和AglaPBP2基因鉴定和表达分析 [J]. 应用昆虫学报, 2017, 54 (1): 45-55]
Wang XS, Tang LD, Wu JH. Research progress on olfactory proteins of insect [J]. Chinese Journal of Tropical Crops, 2017, 38 (6): 1171-1179. [王晓双, 唐良德, 吴建辉. 昆虫嗅觉相关蛋白的研究进展 [J]. 热带作物学报, 2017, 38 (6): 1171-1179]
Weng XQ, Zheng RP, Huang CY. Insect olfactory chemosensory protein and interaction model [J]. Agricultural Science & Technology and Equipment, 2017, 5: 15-16. [翁小倩, 郑茹萍, 黄晨燕. 昆虫嗅觉化学感受蛋白及其相关作用模型 [J]. 农业科技与装备, 2017, 5: 15-16]
Wu HX, Zheng YL, Han MH, et al. Large-scale application of mass trapping of Spodoptera litura with sex pheromone an devaluation of its control efficiency in the cabbage field [J]. Plant Protection, 2011, 37 (1): 138-141. [吴华新, 郑永利, 韩敏晖, 等. 大面积应用性信息素诱捕甘蓝斜纹夜蛾及田间控害效果 [J]. 植物保护, 2011, 37 (1): 138-141]
Xiu WM, Zhou YZ, Dong SL. Molecular characterization and expression pattern of two pheromone-binding proteins from Spodoptera litura (Fabricius) [J].Journal of Chemical Ecology, 2008, 34: 487-498.
Yang A, Guo L, Jiang JX, et al. Overview on characteristics and function of odorant binding proteins in insects [J]. Journal of Changjiang Vegetables, 2018, 2: 36-41. [杨安, 郭玲, 蒋杰贤, 等. 昆虫气味结合蛋白特性与功能研究概述 [J]. 长江蔬菜, 2018, 2: 36-41]
Zhang N, Chen JL, Wu JX. Improvement of semi-artificial diet for Spodoptera litura [J]. Journal of Northwest A & F university (Natural Science Edition), 2016, 4: 109-113. [张南, 陈巨龙, 仵均祥. 斜纹夜蛾半人工饲料的改进 [J]. 西北农林科技大学学报(自然科学版), 2016, 4: 109-113]
Zhang S, Zhang YJ, Su HH, et al. Cloning, expression and tissue-specific expression of cDNA encoding pheromone binding protein PBP2 in Helicoverpa armigera (Hübner) [J]. Scientia Agricultura Sinica, 2009, 42 (7): 2359-2365. [张帅, 张永军, 苏宏华, 等. 棉铃虫信息素结合蛋白2 cDNA的克隆、表达与组织特异性表达 [J]. 中国农业科学, 2009, 42 (7): 2359-2365]
Zhang SX, Cui WZ, Wang GX, et al. Molecular cloning and evolution analysis of Pheromone-binding Protein (PBP) subfamily genes from wild silkworm Bombyx mandarina [J]. Science of Sericulture, 2009, 35 (4): 776-782. [张升祥, 崔为正, 王更先, 等. 野桑蚕性信息素结合蛋白(PBP)亚家族基因的克隆与进化分析 [J]. 蚕业科学, 2009, 35 (4): 776-782]
Zhang YC, Bu YH, Li FZ. Research on effect of gyplure to prevent and control tobacco Spodoptera litura [J]. Yunnan Agricultural Science and Technology, 2013, 3: 13-15. [张映翠, 布云虹, 李凤芝. 性诱剂防治烟田斜纹夜蛾研究 [J]. 云南农业科技, 2013, 3: 13-15]
Zhang ZK, Zhang Y, Wu SY, et al. Recent advances in odorant binding proteins of insects [J]. Journal of Environmmental Entomolgy, 2017, 39 (3): 713-720. [张治科, 张烨, 吴圣勇, 等. 昆虫气味结合蛋白研究进展 [J]. 环境昆虫学报, 2017, 39 (3): 713-720]
Callahan FE, Vogt RG, Tucker ML, et al. High level expression of "male specific" pheromone binding proteins (PBPs) in the antennae of female noctuiid moths [J]. Insect Biochemistry and Molecular Biology, 2000, 30 (6): 507-514.
Dong K.Structure Characterization and Functional Differentiation of Pheromone Binding Proteins in Cotton Worm Helicoverpa armigera [D]. Beijing: China Agricultural University, 2017. [董昆. 棉铃虫性信息素结合蛋白结构特征及功能分化研究 [D]. 北京:中国农业大学, 2017]
Gong DP, Zhao P, Lin Y, et al. Identification and expression pattern of two pheromone binding proteins in silkworm, Bombyx mori [J]. Acta Entomology Sinica, 2006, 49 (3): 355-362. [龚达平, 赵萍, 林英, 等. 家蚕信息素结合蛋白BmPBP2和BmPBP3基因的初步鉴定及表达分析 [J]. 昆虫学报, 2006, 49 (3): 355-362]
Gu SH. Sex pheromone recognition and immunolocalization of three pheromone binding proteins in the black cutworm moth Agrotis ipsilon [J]. Insect Biochemistry and Molecular Biology, 2013, 43 (3): 237-251.
Gong ZJ, Zhou WW, Yu HZ, et al. Cloning, expression and functional analysis of a general odorant-binding protein 2 gene of the rice striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) [J]. Insect Molecular Biology, 2009, 18 (3): 405-417.
He YM. Green control and integration technology of Spodoptera litura [J]. Scientific Breeding, 2017, 11: 34-35. [何永梅. 斜纹夜蛾绿色防控集成技术 [J]. 科学种养, 2017, 11: 34-35]
Jia XJ, Hao SD, Du YL, et al. cDNA cloning, expression profiling and binding affinity assay of the pheromone binding protein Cpun-PBP1 in the yellow peach moth, Conogethes punctiferalis (Lepidoptera:Crambidae) [J]. Acta Entomology Sinica, 2015, 58 (11): 1167-1176. [贾小俭, 郝少东, 杜艳丽, 等. 桃蛀螟性信息素结合蛋白Cpun-PBP1的cDNA克隆、表达谱及其与配体化合物的结合特性分析 [J]. 昆虫学报, 2015, 58 (11): 1167-1176]
Laue M, Steinbrecht RA. Topochemistry of moth olfactory sensilla [J]. International Journal of Insect Morphology & Embryology, 1997, 26 (3): 217-228.
Leal WS. Odorant reception in insects: Roles of receptors, binding proteins, and degrading enzymes [J]. Annual Review of Entomology, 2013, 58: 373-391.
Li L, Yang WL, Guo XR, et al. Cloning sequence analysis and spatio-temporal expression of a pheromone binding protein 2 (PBP2) gene from Helicoverpa assulta (Guenée)(Lepidoptera: Noctuidae) [J]. Acta Entomology Sinica, 2009, 52 (11): 1199-1205. [李亮, 杨文玲, 郭线茹, 等. 烟夜蛾性信息素结合蛋白2(PBP2)基因的克隆、序列分析与时空表达 [J]. 昆虫学报, 2009, 52 (11): 1199-1205]
Li XT, Luo HY, Chen YW, et al. Control efficiency of different biological control technologies on three major pest insects of tobacco [J]. Crop Research, 2011, 25 (4): 361-365. [李晓婷, 罗华元, 陈月舞, 等. 不同生物防治技术对烟草烟蚜和烟青虫及斜纹夜蛾的防治效果 [J]. 作物研究, 2011, 25 (4): 361-365]
Liu NY. FunctionalDifferentiation of Pheromone Binding Protein /General Odorant-bingding Protein in Spodoptera exigua and Spodoptera litura [D]. Nanjing: Nanjing Agricultural University, 2015. [刘乃勇. 甜菜夜蛾和斜纹夜蛾PBP/GOBP的功能分化研究 [D]. 南京:南京农业大学, 2015]
Liu NY, Liu CC, Dong SL. Functional differentiation of pheromone-binding proteins in the common cutworm Spodoptera litura [J]. Comparative Biochemistry & Physiology Part A Molecular & Integrative Physiology, 2013, 165 (2): 254-262.
Miu S, Zhang L, Wang LH. Study progress of insect odorant-binding protein [J]. Journal of Yunnan University, 2003, 25: 161-165. [缪森, 张龙, 王丽红. 昆虫气味结合蛋白研究进展 [J]. 云南大学学报, 2003, 25: 161-165]
Mohanty S, Zubkov S, Gronenborn AM. The solution NMR structure of Antheraea polyphemus PBP provides new in-sight into pheromone recognition by pheromone-binding proteins [J]. Journal of Molecular Biology, 2004, 337 (2): 443-451.
Mukunda L, Miazzi F, Kaltofen S, et al. Calmodulin modulates insect odorant receptor function [J]. Cell Calcium, 2014, 55 (4): 191-199.
Nie NS, Huang SJ, Long QL. Insecticide-resistance and control strategy for common cutworm, Spodoptera litura (Fab.) [J]. Acta Agriculturae Jiangxi, 2007, 19 (7): 51-54. [聂南生, 黄水金, 龙丘陵. 斜纹夜蛾的抗药性及其治理策略 [J]. 江西农业学报, 2007, 19 (7): 51-54]
Pelosi P, Maida R. Odorant-binding proteins in insects [J]. Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology, 1995, 111 (3): 503-514.
Qian HY, Xu AY. Advances in research on insect pheromone binding proteins [J]. Anhui Agricultural Science Bulletin, 2007, 23: 128-130. [钱荷英, 徐安英. 昆虫信息素结合蛋白研究进展 [J]. 安徽农学通报, 2007, 23: 128-130]
Sang S, Wang Z, Qi JW, et al. Research progresses on pesticide resistance of Spodoptera litura [J]. Journal of Environmmental Entomolgy, 2013, 35 (6): 808-814. [桑松, 王政, 齐江卫, 等. 斜纹夜蛾抗药性研究进展 [J]. 环境昆虫学报, 2013, 35 (6): 808-814]
Sun M, Liu Y, Wang G. Expression patterns and binding properties of three pheromone binding proteins in the diamondback moth, Plutella xyllotella [J]. Journal of Insect Physiology, 2012, 59 (1): 46-55.
Tian Z, Zhang Y. Molecular characterization and functional analysis of pheromone binding protein 1 from Cydia pomonella (L.) [J]. Insect Molecular Biology, 2016, 25 (6): 769-777.
Tian ZQ, Sun LN, Li YY, et al. Research progress of the function of sex pheromone binding protein in insects [J]. Journal of Agriculture, 2017, 7 (9): 14-20. [田志强, 孙丽娜, 李艳艳, 等. 昆虫性信息素结合蛋白功能研究进展 [J]. 农学学报, 2017, 7 (9): 14-20]
Vogt RG, Riddiford LM. Pheromone binding and inactivation by moth antennae [J]. Nature, 1981, 293: 161-163.
Wang JZ, Hu P, Luo YQ, et al. Identification and expression patterns of the pheromone binding protein genes AglaPBP1 and AglaPBP2 in Anoplophora glabripennis (Coleoptera:Cerambycidae) [J]. Chinese Journal of Applied Entomology, 2017, 54 (1): 45-55. [王菁桢, 胡平, 骆有庆, 等. 光肩星天牛性信息素结合蛋白AglaPBP1和AglaPBP2基因鉴定和表达分析 [J]. 应用昆虫学报, 2017, 54 (1): 45-55]
Wang XS, Tang LD, Wu JH. Research progress on olfactory proteins of insect [J]. Chinese Journal of Tropical Crops, 2017, 38 (6): 1171-1179. [王晓双, 唐良德, 吴建辉. 昆虫嗅觉相关蛋白的研究进展 [J]. 热带作物学报, 2017, 38 (6): 1171-1179]
Weng XQ, Zheng RP, Huang CY. Insect olfactory chemosensory protein and interaction model [J]. Agricultural Science & Technology and Equipment, 2017, 5: 15-16. [翁小倩, 郑茹萍, 黄晨燕. 昆虫嗅觉化学感受蛋白及其相关作用模型 [J]. 农业科技与装备, 2017, 5: 15-16]
Wu HX, Zheng YL, Han MH, et al. Large-scale application of mass trapping of Spodoptera litura with sex pheromone an devaluation of its control efficiency in the cabbage field [J]. Plant Protection, 2011, 37 (1): 138-141. [吴华新, 郑永利, 韩敏晖, 等. 大面积应用性信息素诱捕甘蓝斜纹夜蛾及田间控害效果 [J]. 植物保护, 2011, 37 (1): 138-141]
Xiu WM, Zhou YZ, Dong SL. Molecular characterization and expression pattern of two pheromone-binding proteins from Spodoptera litura (Fabricius) [J].Journal of Chemical Ecology, 2008, 34: 487-498.
Yang A, Guo L, Jiang JX, et al. Overview on characteristics and function of odorant binding proteins in insects [J]. Journal of Changjiang Vegetables, 2018, 2: 36-41. [杨安, 郭玲, 蒋杰贤, 等. 昆虫气味结合蛋白特性与功能研究概述 [J]. 长江蔬菜, 2018, 2: 36-41]
Zhang N, Chen JL, Wu JX. Improvement of semi-artificial diet for Spodoptera litura [J]. Journal of Northwest A & F university (Natural Science Edition), 2016, 4: 109-113. [张南, 陈巨龙, 仵均祥. 斜纹夜蛾半人工饲料的改进 [J]. 西北农林科技大学学报(自然科学版), 2016, 4: 109-113]
Zhang S, Zhang YJ, Su HH, et al. Cloning, expression and tissue-specific expression of cDNA encoding pheromone binding protein PBP2 in Helicoverpa armigera (Hübner) [J]. Scientia Agricultura Sinica, 2009, 42 (7): 2359-2365. [张帅, 张永军, 苏宏华, 等. 棉铃虫信息素结合蛋白2 cDNA的克隆、表达与组织特异性表达 [J]. 中国农业科学, 2009, 42 (7): 2359-2365]
Zhang SX, Cui WZ, Wang GX, et al. Molecular cloning and evolution analysis of Pheromone-binding Protein (PBP) subfamily genes from wild silkworm Bombyx mandarina [J]. Science of Sericulture, 2009, 35 (4): 776-782. [张升祥, 崔为正, 王更先, 等. 野桑蚕性信息素结合蛋白(PBP)亚家族基因的克隆与进化分析 [J]. 蚕业科学, 2009, 35 (4): 776-782]
Zhang YC, Bu YH, Li FZ. Research on effect of gyplure to prevent and control tobacco Spodoptera litura [J]. Yunnan Agricultural Science and Technology, 2013, 3: 13-15. [张映翠, 布云虹, 李凤芝. 性诱剂防治烟田斜纹夜蛾研究 [J]. 云南农业科技, 2013, 3: 13-15]
Zhang ZK, Zhang Y, Wu SY, et al. Recent advances in odorant binding proteins of insects [J]. Journal of Environmmental Entomolgy, 2017, 39 (3): 713-720. [张治科, 张烨, 吴圣勇, 等. 昆虫气味结合蛋白研究进展 [J]. 环境昆虫学报, 2017, 39 (3): 713-720]