福建正红菇遗传多样性分析
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摘要
以采自福建省山区的正红菇Russula griseocarnosa为研究材料,进行ITS(internal transcribed spacer)、RPB2(the second largest subunit of the nuclear RNA polymerase enzyme II)保守区段克隆测序和序列同源性比对。将从GenBank中获得的部分红菇属物种ITS和RPB2序列与正红菇序列通过最大简约法进行系统发育分析。基于ITS序列构建的系统发育树表明,福建正红菇与云南大红菌Russula griseocarnosa X.H.Wang,Zhu L.Yang&Knudsen,sp.nov.间序列差异较小,亲缘关系较近,以99%的支持率聚为一簇。福建正红菇与欧洲红菇Russula vinosa及玫瑰红菇Russula rosea间序列差异较大,亲缘关系较远,在系统发育树上聚为不同分枝。基于RPB2序列构建的系统发育树也表明福建正红菇与云南大红菌聚为一簇(支持率94%),而与玫瑰红菇聚为不同分支。
Fruiting bodies of Russula griseocarnosa were collected in Fujian to study the mushroom′s phylogenetic diversity.Two gene regions of ITS(internal transcribed spacer)and RPB2(the second largest subunit of the nuclear RNA polymerase enzyme II)were cloned.The phylogenetic diversity was examined according to the maximum parsimony trees with sequences of several other Russula species.It showed that,based on the ITS sequences,R.griseocarnosafound in the province clustered with R.griseocarnosa X.H.Wang,Zhu L.Yang & Knudsen,sp.nov.found in Yunnan,with a bootstrap value of 99%.The differences among R.griseocarnosa,R.vinosa and R.rosea were apparent,with a bootstrap value below 50%.The maximum parsimony tree based on the RPB2 sequences also showed that R.griseocarnosa clustered with R.griseocarnosa X.H.Wang,Zhu L.Yang & Knudsen,with a bootstrap value of 94%.The distinction was also apparent,with a bootstrap value less than 50%.
Fruiting bodies of Russula griseocarnosa were collected in Fujian to study the mushroom′s phylogenetic diversity.Two gene regions of ITS(internal transcribed spacer)and RPB2(the second largest subunit of the nuclear RNA polymerase enzyme II)were cloned.The phylogenetic diversity was examined according to the maximum parsimony trees with sequences of several other Russula species.It showed that,based on the ITS sequences,R.griseocarnosafound in the province clustered with R.griseocarnosa X.H.Wang,Zhu L.Yang & Knudsen,sp.nov.found in Yunnan,with a bootstrap value of 99%.The differences among R.griseocarnosa,R.vinosa and R.rosea were apparent,with a bootstrap value below 50%.The maximum parsimony tree based on the RPB2 sequences also showed that R.griseocarnosa clustered with R.griseocarnosa X.H.Wang,Zhu L.Yang & Knudsen,with a bootstrap value of 94%.The distinction was also apparent,with a bootstrap value less than 50%.
引文
[1]李国杰,文华安.中国红菇属分类研究进展[J].菌物学报,2009,28(2):303-309.
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[3]CHEN X H,CHEN L X,ZHOU H B,et al.Chemical Composition and Antioxidant Activities of Russula griseocarnosa sp.Nov[J].Journal of Agricltural and Food Chemistry,2010,58:6966-6971.
[4]唐超,陈应龙,刘润进.菌根食用菌研究进展[J].菌物学报,2011,30(3):367-378.
[5]WANG X H,YANG Z L,LI Y C,et al.Russula griseocarnosa sp.nov.(Russulaceae,Russulales),a commercially important edible mushroom in tropical China:mycorrhiza,phylogenetic position,and taxonomy[J].Nova Hedwigia,2009,88:269-282.
[6]SCHMITT I,CRESPO A,DIVAKAR P K.New primers for promising single-copy genes in fungal phylogenetics and systematic[J].Persoonia,2009,23:35-40.
[7]ALVIN M C,RAJESH J,KEVIN D H.Phylogenetic utility of protein(RPB2,b-tubulin)and ribosomal(LSU,SSU)gene sequences in the systematic of Sordariomycetes(Ascomycota,Fungi)[J].Antonie Van Leeuwenhoek,2007,91:327-349.
[8]陈新华.广东商品红菇形态和分子鉴定、营养成分分析及其生物活性研究[D].湖南:中南大学,2010.
[9]王桂文,刘文波.广西红菇子实体及分离株的rDNA ITS序列分析[J].广西科学,2004,11(3):261-265.
[2]李惠珍,黄德鑫,许旭萍.红菇属的生物化学研究进展[J].中国食用菌,1999,18(4):8-10.
[3]CHEN X H,CHEN L X,ZHOU H B,et al.Chemical Composition and Antioxidant Activities of Russula griseocarnosa sp.Nov[J].Journal of Agricltural and Food Chemistry,2010,58:6966-6971.
[4]唐超,陈应龙,刘润进.菌根食用菌研究进展[J].菌物学报,2011,30(3):367-378.
[5]WANG X H,YANG Z L,LI Y C,et al.Russula griseocarnosa sp.nov.(Russulaceae,Russulales),a commercially important edible mushroom in tropical China:mycorrhiza,phylogenetic position,and taxonomy[J].Nova Hedwigia,2009,88:269-282.
[6]SCHMITT I,CRESPO A,DIVAKAR P K.New primers for promising single-copy genes in fungal phylogenetics and systematic[J].Persoonia,2009,23:35-40.
[7]ALVIN M C,RAJESH J,KEVIN D H.Phylogenetic utility of protein(RPB2,b-tubulin)and ribosomal(LSU,SSU)gene sequences in the systematic of Sordariomycetes(Ascomycota,Fungi)[J].Antonie Van Leeuwenhoek,2007,91:327-349.
[8]陈新华.广东商品红菇形态和分子鉴定、营养成分分析及其生物活性研究[D].湖南:中南大学,2010.
[9]王桂文,刘文波.广西红菇子实体及分离株的rDNA ITS序列分析[J].广西科学,2004,11(3):261-265.