一株采自四川南充的羊肚菌生境调查与鉴定
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摘要
为调查四川南充羊肚菌资源并考察其发生地环境,于2014年3月31日在南充市西充县金泉乡双桥子村采集到1株羊肚菌.通过菌丝体培养,镜检观察完成形态鉴定,基于ITS、LSU、ef1-α、rpb1和rpb2序列分析构建的系统发育树完成分子鉴定,确定为Morchella sp.(Mes-16),归入黄色羊肚菌支系(Esculenta Clade),进一步结合样地环境及土壤理化性质分析了羊肚菌在当季发生的可能的机制.
In order to investigate the resource and habitat of morel in Nanchong,Sichuan,1 strain of morel was collected from Shuangqiaozi Village,Nanchong,Sichuan,China,in March 2014. According to morphological evidence and DNA sequences of ITS,LSU,ef1-α,rpb1 and rpb2 sequences,all samples were characterized as Morchella sp.( Mes-16),belonging to Esculenta Clade. Further analysis of the environment of morels habitat and the local soil sample indicated the possible mechanism for this autumn occurrence of morels.
In order to investigate the resource and habitat of morel in Nanchong,Sichuan,1 strain of morel was collected from Shuangqiaozi Village,Nanchong,Sichuan,China,in March 2014. According to morphological evidence and DNA sequences of ITS,LSU,ef1-α,rpb1 and rpb2 sequences,all samples were characterized as Morchella sp.( Mes-16),belonging to Esculenta Clade. Further analysis of the environment of morels habitat and the local soil sample indicated the possible mechanism for this autumn occurrence of morels.
引文
[1]杜习慧,赵琪,杨祝良.羊肚菌的多样性、演化历史及栽培研究进展[J].菌物学报,2014,33(2):183-197.
[2]WIPF D,FRIBOURG A,MUNCH J C.Diversity of the internal transcribed spacer of r DNA in morels[J].American Journal of Botany,1999,45(9):769-778.
[3]KUO M,DEWSBURY D R,O'Donnell K,et al.Taxonomic revision of true morels(Morchella)in Canada and the United States[J].Mycologia,2012,104(5):1159-1177.
[4]TAYLOR J W,JACOBSON D,KROKEN S,et al.Phylogenetic species recognition and species concepts in fungi[J].Fungal Genetics and Biology,2000,31(1):21-32.
[5]DU XH,ZHAO Q,O'DONNELL K,et al.Multigene molecular phylogenetics reveals true morels(Morchella)are especiallyspecies-rich in China[J].Fungal Genetics and Biology,2012a,49:455-469.
[6]王昭艳,左长清,曹文洪,等.2011红壤丘陵区不同植被恢复模式土壤理化性质相关分析[J].土壤学报,48(4):715-724.
[7]鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社,2000,34-35,81-83,106-107.
[8]邵蕾,王丽霞,张民,等.控释肥类型及氮素水平对氮磷钾利用率的影响[J].水土保持学报,2009,23(4):170-175.
[9]孟珍贵,李永,赵琪,等.滇西北松口蘑菌塘的生态因子调查[J].食用菌学报,2011,18(1):12-17.
[10]Kuo M.Morchella tomentosa,a new species from western North America,and notes on M.rufobrunnea[J].Mycotaxon,2008,105:441-446.
[11]DU X H,ZHAO Q,YANG Z L,et al.How well do ITS r DNA sequences differentiate species of true morels(Morchella)?[J].Mycologia,2012b,104(6):1351-1368.
[12]TAMURA K,PETERSON D,PETERSON N,et al.MEGA5:Molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[13]KAUSERUD H,SCHUMACHER T.Outcrossing or inbreeding:DNA markers provide evidence for type of reproductive mode in Phellinus nigrolimitatus(Basidiomycota)[J].Mycological Research,2001,105:676-683.
[14]WTITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal RNA for phylogenetics[J].PCR Protocols.Academic Press,San Diego,1990:315-322.
[15]O'DONNELL K,CIGELNIK E,WEBER N S,et al.Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18 S and 28 S ribosomal DNA sequence analysis[J].Mycologia,1997,89:48-65.
[16]MORTIMER P E,KARUNARATHNA S C,LI Q H,et al.Prized edible Asian mushrooms:ecology,conservation and sustainability[J].Fungal Diversity,2012,56:31-47.
[17]O'DONNELL K,ROONEY A P,MILLS G L,et al.Phylogeny and historical biogeography of true morels(Morchella)reveals an early Cretaceous origin and high continental endemism and provincialism in the Holarctic[J].Fungal Genetics and Biology,2011,48(3):252-265.
[18]赵琪,徐中志,程远辉,等.尖顶羊肚菌仿生栽培技术[J].西南农业学报,2009,22(6):1690-1693.
[19]BUSCOT F.Field observations on growth and development of Morchella rotunda and Mitrophora semilibera in relation to forest soil temperature[J].Canadian Journal of Botany,1989,67(11):589-593.
[20]MILLER S C.Cultivation of Morchella:United States,6951074[P].2002-06-26.
[21]李青连,丁翠,范黎.羊肚菌营养方式的稳定碳同位素研究[J].菌物学报,2013,32(Z1):213-223.
[22]王波,鲜灵.人工栽培羊肚菌的鉴定[J].西南农业学报,2013,26(5):1988-1991.
[2]WIPF D,FRIBOURG A,MUNCH J C.Diversity of the internal transcribed spacer of r DNA in morels[J].American Journal of Botany,1999,45(9):769-778.
[3]KUO M,DEWSBURY D R,O'Donnell K,et al.Taxonomic revision of true morels(Morchella)in Canada and the United States[J].Mycologia,2012,104(5):1159-1177.
[4]TAYLOR J W,JACOBSON D,KROKEN S,et al.Phylogenetic species recognition and species concepts in fungi[J].Fungal Genetics and Biology,2000,31(1):21-32.
[5]DU XH,ZHAO Q,O'DONNELL K,et al.Multigene molecular phylogenetics reveals true morels(Morchella)are especiallyspecies-rich in China[J].Fungal Genetics and Biology,2012a,49:455-469.
[6]王昭艳,左长清,曹文洪,等.2011红壤丘陵区不同植被恢复模式土壤理化性质相关分析[J].土壤学报,48(4):715-724.
[7]鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社,2000,34-35,81-83,106-107.
[8]邵蕾,王丽霞,张民,等.控释肥类型及氮素水平对氮磷钾利用率的影响[J].水土保持学报,2009,23(4):170-175.
[9]孟珍贵,李永,赵琪,等.滇西北松口蘑菌塘的生态因子调查[J].食用菌学报,2011,18(1):12-17.
[10]Kuo M.Morchella tomentosa,a new species from western North America,and notes on M.rufobrunnea[J].Mycotaxon,2008,105:441-446.
[11]DU X H,ZHAO Q,YANG Z L,et al.How well do ITS r DNA sequences differentiate species of true morels(Morchella)?[J].Mycologia,2012b,104(6):1351-1368.
[12]TAMURA K,PETERSON D,PETERSON N,et al.MEGA5:Molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[13]KAUSERUD H,SCHUMACHER T.Outcrossing or inbreeding:DNA markers provide evidence for type of reproductive mode in Phellinus nigrolimitatus(Basidiomycota)[J].Mycological Research,2001,105:676-683.
[14]WTITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal RNA for phylogenetics[J].PCR Protocols.Academic Press,San Diego,1990:315-322.
[15]O'DONNELL K,CIGELNIK E,WEBER N S,et al.Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18 S and 28 S ribosomal DNA sequence analysis[J].Mycologia,1997,89:48-65.
[16]MORTIMER P E,KARUNARATHNA S C,LI Q H,et al.Prized edible Asian mushrooms:ecology,conservation and sustainability[J].Fungal Diversity,2012,56:31-47.
[17]O'DONNELL K,ROONEY A P,MILLS G L,et al.Phylogeny and historical biogeography of true morels(Morchella)reveals an early Cretaceous origin and high continental endemism and provincialism in the Holarctic[J].Fungal Genetics and Biology,2011,48(3):252-265.
[18]赵琪,徐中志,程远辉,等.尖顶羊肚菌仿生栽培技术[J].西南农业学报,2009,22(6):1690-1693.
[19]BUSCOT F.Field observations on growth and development of Morchella rotunda and Mitrophora semilibera in relation to forest soil temperature[J].Canadian Journal of Botany,1989,67(11):589-593.
[20]MILLER S C.Cultivation of Morchella:United States,6951074[P].2002-06-26.
[21]李青连,丁翠,范黎.羊肚菌营养方式的稳定碳同位素研究[J].菌物学报,2013,32(Z1):213-223.
[22]王波,鲜灵.人工栽培羊肚菌的鉴定[J].西南农业学报,2013,26(5):1988-1991.