中国新记录种毛细木霉Trichoderma capillare及功能评价
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  • 英文篇名:Chinese new record Trichoderma capillare and its functional assessment
  • 作者:陈凯 ; 李纪顺 ; 张广志 ; 王贻莲 ; 赵晓燕 ; 吴晓青 ; 扈进冬 ; 杨合同
  • 英文作者:CHEN Kai;LI Ji-shun;ZHANG Guang-zhi;WANG Yi-lian;ZHAO Xiao-yan;WU Xiao-qing;HU Jin-dong;YANG He-tong;Shandong Provincial Key Laboratory of Applied Microbiology,Biology Institute,Biotechnology Center,Shandong Academy of Sciences;
  • 关键词:中国新记录种 ; 毛细木霉 ; 抑菌能力 ; 水解酶活性
  • 英文关键词:Chinese new record;;Trichoderma capillare;;antifungal capability;;hydrolase activity
  • 中文刊名:SDKX
  • 英文刊名:Shandong Science
  • 机构:山东省科学院生物研究所山东省科学院生物技术研究中心山东省应用微生物重点实验室;
  • 出版日期:2016-02-15
  • 出版单位:山东科学
  • 年:2016
  • 期:v.29;No.138
  • 基金:山东省重点研发计划(2015GNC113003);; 山东省攻关计划(2014GSF121028);; 科技基础性工作专项(2014FY120900)
  • 语种:中文;
  • 页:SDKX201601016
  • 页数:6
  • CN:01
  • ISSN:37-1188/N
  • 分类号:97-101+113
摘要
木霉菌株TW22166分离自湖南省洞庭湖滩涂湿地,该菌株分生孢子梗具有长而发达的主轴,二次分枝丰富,不规则,一般单生,分生孢子梗向顶方向着生单个瓶梗。利用ITS、tef1及rpb2序列分析,发现该菌株同Trichoderma capillare同源性达到99%以上;在以rpb2构建的系统发育进化树中,TW22166与Trichoderma capillare strain GJS 06-66[JN175530.1]位于同一分支。该菌株在PDA平板上对9种植物病原真菌均有抑制作用,其中对Fusarium.oxysporum的抑制率为100.0%。菌株具有蛋白酶、几丁质酶及纤维素酶活性,其中蛋白酶活性较高,透明圈宽度达16.0 mm。该菌株鉴定为毛细木霉Trichoderma capillare,是木霉属中国新记录种,也是一株较有潜力的生物防治菌株。
        Trichoderma strain TW22166 was isolated from soil samples of Dongting Lake mudflat wetland of Hunan province,China. Its conidiophores had a strongly developed central axis from which phialides arise singly over several levels below the tip a single phialide terminating a basal cell with a short,spur-like phialide arising as an outgrowth of the basal cell at the septum. Through detecting molecular markers( ITS,tef1 and rpb2),we discovered that its homology with Trichoderma capillare was more than 99% by NCBI. In rpb2 sequences based phylogenetic tree,strain TW22166 and Trichoderma capillare strain GJS 06-66 [JN175530. 1] were on one identical branch. Strain TW22166 exhibited antifungal capability against 9 plant pathogenic fungi on PDA plate,and its inhibition ratio against Fusarium oxysporum was up to100. 0%. It had proteinase, chitinase and cellulase activity, among which proteinase activity was higher and its hydrolyzation radius was 16. 0 mm. Strain TW22166 was identified as Trichoderma capillare,a Chinese new record of genus Trichoderma which was a potential biological prevention and control strain.
引文
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