16S rRNA技术检测盐诱导高血压大鼠肠道菌群的变化
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摘要
目的检测高血压大鼠肠道菌群的变化,探讨正常菌群在盐诱导高血压发生发展中的作用。方法以8%高盐饮食喂养SD雄性大鼠制备高血压模型。Real-time PCR检测菌群结构的改变,同时检测血浆炎性因子IL-1β、IL-6和TNF-α水平变化。结果同对照组大鼠血压(96.00mmHg±5.74mmHg)相比盐敏感组(122.79 mmHg±6.37 mmHg)显著升高,而盐抵抗组无明显变化;实验组大鼠体重(172.00g±15.58g,164.25g±16.11g)较对照组(377.63g±32.47g)明显降低;同对照组相比实验组菌群结构发生比例倒置,即双歧杆菌(6.19±0.47,7.52±0.47 vs 8.59±0.42)、乳杆菌(6.77±0.23,7.09±0.28 vs 7.60±0.26)、拟杆菌(8.98±0.45,8.46±0.47 vs 9.99±0.73)数量降低;肠杆菌(7.93±0.20,7.78±0.29 vs 7.28±0.27)数量升高。同盐抵抗组大鼠相比,盐敏感组双歧杆菌、乳杆菌降低更加显著,但拟杆菌数量高于盐抵抗组。两实验组大鼠血浆细胞因子IL-1β、IL-6和TNF-α水平较对照组均显著升高(P<0.05)。结论盐诱导高血压大鼠肠道菌群结构发生改变,盐敏感组双歧杆菌、乳杆菌和拟杆菌含量显著降低,提示其可能参与盐诱导高血压病程。
Objective To observe the changes of the intestinal flora structure in high salt induced hypertension rats.Methods SD male rats were fed on 8% NaCl diet for 8 weeks to prepare hypertension models.Real-time PCR method was used to detect the changes of the flora structure for analysis on the role of intestinal flora in the process of salt sensitive hypertension.Meanwhile,the levels of IL-1β,IL-6 and TNF-αin plasma were detected.Results Compared with the control group(96.00 mmHg±5.74 mmHg),the blood pressure in the salt sensitivity group(122.79 mmHg±6.37 mmHg)increased significantly,while there was no significant change in the salt resistance group.Compared with the control group(377.63 g±32.47 g),the body weight in the experiment group(172.00 g±15.58 g,164.25 g±16.11 g)decreased significantly.The structure of microflora in the experiment group was changed,the numbers of Bifidobacteria(6.19±0.47,7.52±0.47 vs 8.59±0.42),Lactobacilli(6.77±0.23,7.09±0.28 vs 7.60±0.26)and Escherichia coli(8.98±0.45,8.46±0.47 vs 9.99±0.73)decreased,while that of Enterobacters(7.93±0.20,7.78±0.29 vs 7.28±0.27)increased.Compared with the salt resistance group,the reduction of Bifidobacteria and Lactobacilli in the salt sensitivity group were more significant,but that of Escherichia coli was higher than in the salt resistance group.The levels of plasma cytokines IL-1β,IL-6 and TNF-αin the experiment group were significantly higher than those in the control group(P<0.05).Conclusion The structure of intestinal microflora in salt induced hypertension rats changed.The contents of Bifidobacteria,Lactobacilli and Bacteroides decreased significantly in salt sensitivity group,suggesting that it might play an important role in salt induced hypertension.
Objective To observe the changes of the intestinal flora structure in high salt induced hypertension rats.Methods SD male rats were fed on 8% NaCl diet for 8 weeks to prepare hypertension models.Real-time PCR method was used to detect the changes of the flora structure for analysis on the role of intestinal flora in the process of salt sensitive hypertension.Meanwhile,the levels of IL-1β,IL-6 and TNF-αin plasma were detected.Results Compared with the control group(96.00 mmHg±5.74 mmHg),the blood pressure in the salt sensitivity group(122.79 mmHg±6.37 mmHg)increased significantly,while there was no significant change in the salt resistance group.Compared with the control group(377.63 g±32.47 g),the body weight in the experiment group(172.00 g±15.58 g,164.25 g±16.11 g)decreased significantly.The structure of microflora in the experiment group was changed,the numbers of Bifidobacteria(6.19±0.47,7.52±0.47 vs 8.59±0.42),Lactobacilli(6.77±0.23,7.09±0.28 vs 7.60±0.26)and Escherichia coli(8.98±0.45,8.46±0.47 vs 9.99±0.73)decreased,while that of Enterobacters(7.93±0.20,7.78±0.29 vs 7.28±0.27)increased.Compared with the salt resistance group,the reduction of Bifidobacteria and Lactobacilli in the salt sensitivity group were more significant,but that of Escherichia coli was higher than in the salt resistance group.The levels of plasma cytokines IL-1β,IL-6 and TNF-αin the experiment group were significantly higher than those in the control group(P<0.05).Conclusion The structure of intestinal microflora in salt induced hypertension rats changed.The contents of Bifidobacteria,Lactobacilli and Bacteroides decreased significantly in salt sensitivity group,suggesting that it might play an important role in salt induced hypertension.
引文
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[2]Santisteban MM,Qi Y,Zubcevic J,et al.Hypertension-linked pathophysiological alterations in the gut[J].Circul Res,2017,120(2):312-323.
[3]Pluznick JL,Protzko RJ,Gevorgyan H,et al.Olfactory receptor responding to gut microbiota-derived signals plays a role in renin secretion and blood pressure regulation[J].Proc Natl Acad Sci U S A,2013,110(11):4410-4415.
[4]Li J,Zhao F,Wang Y,et al.Gut microbiota dysbiosis contributes to the development of hypertension[J].Microbiome,2017,5(1):14.
[5]Gómez-Guzmán M,Toral M,Romero M,et al.Antihypertensive effects of probiotics Lactobacillus strains in spontaneously hypertensive rats[J].Mol Nutr Food Res,2015,59(11):2326-2336.
[6]Yang T,Santisteban MM,Rodriguez V,et al.Gut dysbiosis is linked to hypertension[J].Hypertension,2015,65(6):1331-1340.
[7]Mell B,Jala VR,Mathew AV,et al.Evidence for a link between gut microbiota and hypertension in the Dahl rat[J].Physiol Genomics,2015,47(6):187-197.
[8]Cani PD,Delzenne NM,Amar J,et al.Role of gut microflora in the development of obesity and insulin resistance following high-fat diet feeding[J].Pathol Biol,2008,56(5):305-309.