Miseq测序分析围垦后海三棱藨草湿地土壤微生物群落多样性的季节变化
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摘要
为探讨上海南汇东滩湿地围垦后海三棱藨草土壤微生物群落多样性的季节变化,应用Miseq高通量测序技术,分析藨草密集区与光滩区4个季度土壤微生物群落多样性以及相关的土壤环境因子。结果表明,藨草密集区与光滩区土壤微生物群落多样性及丰富度差异显著(P <0. 001),藨草密集区高于光滩区,同一区域样本微生物多样性及丰富度季节变化显著(P <0. 001),春夏2季低于秋冬2季。Proteobacteria(变形菌门)、Bacteroidetes(拟杆菌门)、Acidobacteria(酸杆菌门)、Chloroflexi(绿弯菌门)、Planctomycetes(浮霉菌门)、Actinobacteria(放线菌门)、Cyanobacteria(蓝藻菌门)是各样本中相对丰度较高的菌门,且样品优势菌季节性差异较大。RDA冗余分析表明,土壤盐分、干湿比、硝态氮是影响土壤微生物群落结构的主要因素。研究对弄清海三棱藨草湿地的空间扩张微观机制及促进海三棱藨草湿地快速修复具有理论意义。
By Miseq high-throughput sequencing technology,soil microbial community dynamics and the associated soil physiochemical characteristics were studied.The reclaimed Scirpus mariqueter wetland in Shanghai Nanhui Dongtan coastal area was the study site,which is just undergoing large scale wetlands reclamation impacts.Results showed the dense area and the light beach of S.mariqueter soil microbial communities were significantly different(P<0.001).The dense area of S.mariqueter is higher than the light beach area.The seasonal variation of microbial diversity and abundance in the same area was significant(P<0.001),and the spring and summer microbial diversity and abundance was lower than that in the autumn and winter.Proteobacteria,Bacteroidetes,Acidobacteria,Chloroflexi,Planctomycetes,Actinobacteria,Cyanobacteria,Gemmatimonadetes,and Nitrospirae were relatively abundant,and the seasonal dominant microbial community was different.Redundancy analysis showed that microbial community structure was significantly correlated with factors including soil salinity,dry wet ratio and nitrate nitrogen.This study is helpful to understand the micromechanism during S.mariqueter wetlands spatial sprawling and promote the fast remediation of S.mariqueter wetlands.
By Miseq high-throughput sequencing technology,soil microbial community dynamics and the associated soil physiochemical characteristics were studied.The reclaimed Scirpus mariqueter wetland in Shanghai Nanhui Dongtan coastal area was the study site,which is just undergoing large scale wetlands reclamation impacts.Results showed the dense area and the light beach of S.mariqueter soil microbial communities were significantly different(P<0.001).The dense area of S.mariqueter is higher than the light beach area.The seasonal variation of microbial diversity and abundance in the same area was significant(P<0.001),and the spring and summer microbial diversity and abundance was lower than that in the autumn and winter.Proteobacteria,Bacteroidetes,Acidobacteria,Chloroflexi,Planctomycetes,Actinobacteria,Cyanobacteria,Gemmatimonadetes,and Nitrospirae were relatively abundant,and the seasonal dominant microbial community was different.Redundancy analysis showed that microbial community structure was significantly correlated with factors including soil salinity,dry wet ratio and nitrate nitrogen.This study is helpful to understand the micromechanism during S.mariqueter wetlands spatial sprawling and promote the fast remediation of S.mariqueter wetlands.
引文
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