高F值寡肽对小鼠肠道菌群影响探究
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摘要
为探究高F值寡肽对改善肠道吸收状况的影响。选用昆明种小鼠为实验对象,将其分为高F值寡肽酶解液组、超滤组和对照组,分别每天灌胃,10 d后收集小鼠肠道内容物,通过试剂盒提取DNA并利用16S rRNA技术获得各组小鼠肠道的优势菌群并采用Anosim、OTU Venn、OTU PCA、PCoA和LDA EffectSize (LEfSe)等进行分析。结果表明,酶解液组优势菌群为蛭弧菌科与毛螺菌科,超滤组为肠球菌科与嗜冷杆菌属,而对照组为拟杆菌门的黄杆菌目和拟杆菌科、β-变形菌纲的黄杆菌科与产碱杆菌科。高F值寡肽酶解液可以改变肠道菌群的整体结构,并能增加肠道内的有益菌种,从而维持肠道菌群的结构和稳定。
To explore the effect of high fischer ratio oligo-peptides on the improvement of intestinal absorption. Kunming mice were selected as experimental subjects and divided into high fischer ratio oligo-peptides hydrolyzing solution group, ultrafiltration group and control group. They were intragastrically administrated on a daily basis, and the intestinal contents of mice were collected ten days later. DNA was extracted by the kit. The 16 S rRNA technology was used to obtain the dominant intestinal flora of mice in each group and analyzed using Anosim, OTU Venn, OTU PCA, PCoA, and LDA EffectSiz e(LEfSe). The results showed that the dominant bacteria group in the enzymolysis solution group were Bdellovibrio and Lachnospiraceae, the ultrafiltration group was Enterococcaceae and Psychrobacter, and the control group was Flavobacteriales and Bacteroidaceae of Bacteroidetes, Flavobacteriums and Alcaligenacea of Beta-proteobacteria. The high fischer ratio oligo-peptides hydrolysate can change the overall structure of the intestinal flora and increase the probiotic bacteria in the intestine, thereby maintaining the structure and stability of the intestinal flora.
To explore the effect of high fischer ratio oligo-peptides on the improvement of intestinal absorption. Kunming mice were selected as experimental subjects and divided into high fischer ratio oligo-peptides hydrolyzing solution group, ultrafiltration group and control group. They were intragastrically administrated on a daily basis, and the intestinal contents of mice were collected ten days later. DNA was extracted by the kit. The 16 S rRNA technology was used to obtain the dominant intestinal flora of mice in each group and analyzed using Anosim, OTU Venn, OTU PCA, PCoA, and LDA EffectSiz e(LEfSe). The results showed that the dominant bacteria group in the enzymolysis solution group were Bdellovibrio and Lachnospiraceae, the ultrafiltration group was Enterococcaceae and Psychrobacter, and the control group was Flavobacteriales and Bacteroidaceae of Bacteroidetes, Flavobacteriums and Alcaligenacea of Beta-proteobacteria. The high fischer ratio oligo-peptides hydrolysate can change the overall structure of the intestinal flora and increase the probiotic bacteria in the intestine, thereby maintaining the structure and stability of the intestinal flora.
引文
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[24]李桂军,楼永良.脆弱拟杆菌肠毒素致病机制研究进展[J].中国人兽共患病学报, 2009, 25(1):70-72; 77.
[25]石青峰,高玲. ICU脑膜炎败血黄杆菌所致医院获得性感染的临床特征及耐药性分析[J].国际检验医学杂志, 2013, 34(1):27-28; 31.
[26]黎雪梅,李小兵,郑宗林,等.水生动物柱状黄杆菌的研究进展[J].饲料与畜牧, 2014(6):31-37.
[27]程灿灿.住院患者粪便中产NDM超级细菌调查及基因分型研究[D].广州:南方医科大学, 2014.
[2] LEE Y K, MAZMANIAN S K. Has the microbiota played a critical role in the evolution of the adaptive immune system?[J].Science, 2010, 330(6012):1 768-1 773.
[3] ZHU Yuanmin, LUO T M, JOBIN C, et al. Gut microbiota and probiotics in colon tumorigenesis[J]. Cancer Letters, 2011, 309(2):119-127.
[4] EDGAR R C, HAAS B J, CLEMENTE J C, et al. UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics, 2011, 27(16):2 194-2 200.
[5] EDGAR R C. UPARSE:Highly accurate OUT sequences from microbial amplicon reads[J]. Nature Methods, 2013, 10(10):996-998.
[6] ZHENG Weiwei, ZHANG Ze, LIU Cuihua, et al. Metagenomic sequencing reveals altered metabolic pathways in the oral microbiota of sailors during a long sea voyage[J]. Scientific Reports, 2015, 5:9 131.
[7] SANDERS J G, BEICHMAN A C, ROMAN J, et al. Baleen whales host a unique gut microbiome with similarities to both carnivores and herbivores[J]. Nature Communications, 2015, 6:8 285.
[8] XU Jia, LIAN Fengmei, ZHAO Linhua, et al. Stryctural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula[J]. The ISME Journal, 2015, 9(3):552-562.
[9] YASUDA K, OH K, REN B, et al. Biogeography of the intestinal mucosal and lumenalmicrobiome in the rhesus macaque[J].Cell Host and Microbe, 2015, 17(3):385-391.
[10] MOHD SHAUFI M A, SIEO C C, CHONG C W, et al. Deciphering chicken gut microbial dynamics based on high-throughput16S r RNA metagenomics analyses[J]. Gut Pathogens, 2015, 7(1):4.
[11] ECKBURG P B, BIK E M, BERNSTEIN C N, et al. Diversity of the Human Intestinal Microbial Flora[J]. Science, 2005, 308(5728):1 635-1 638.
[12]王恩辉,张晓黎,张莹,等.山东半岛北岸不同生境潮间带浮游细菌多样性研究[J].海洋科学, 2016, 40(6):8-16.
[13]王婷婷.肠道菌群结构变化与结直肠癌发生发展关系的研究[D].上海:上海交通大学, 2012.
[14]潘明芳.植物乳杆菌ZDY2013对小鼠胃肠道微生态的影响及其对幽门螺杆菌感染的预防作用[D].南昌:南昌大学, 2016.
[15]赵立平,费娜.肠道菌群与肥胖症的关系研究进展[J].微生物与感染, 2013, 8(2):61-71.
[16] LEY R E, LOZUPONE C A, HAMADY M, et al. Worlds within worlds:evolution of the vertebrate gut microbiota[J]. Nature Reviews Microbiology, 2008, 6(10):776-788.
[17] ZHANG Chenhong, ZHANG Menghui, PANG Xiaoyan, et al. Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations[J]. The ISME Journal, 2012, 6(10):1 848-1 857.
[18] TURNBAUGH P J, BCKHED F, FULTON L, et al. Diet-Induced Obesity Is Linked to Marked but Reversible Alterations in the Mouse Distal Gut Microbiome[J]. Cell Host and Microbe, 2008, 3(4):213-223.
[19] LEBBA V, SANTANGELO F, TOTINO V, et al. Higher prevalence and abundance of Bdellovibrio bacteriovorus in the human gut of healthy subjects[J]. PloS One, 2013, 8(4):e61608.
[20] LOOZEN G, BOON N, PAUWELS M, et al. Effect of Bdellovibrio bacteriovorus HD100 on multispecies oral communities[J].Anaerobe, 2015, 35(Pt A):45-53.
[21] MEIJER K, BE VOS P, PRIEBE M G. Butyrate and other short-chain fatty acids as modulators of immunity:what relevance for health[J]. Current Opinion in Clinical Nutrition and Metabolic Care, 2010, 13(6):715-721.
[22]陈曦,李英英,宋铁英.单一益生菌(屎肠球菌)介导的断奶仔猪肠道菌群变化规律[J].福建农业学报, 2016, 31(10):1 091-1 097.
[23]杨红玲,马如龙,孙云章.石斑鱼肠道原籍嗜冷杆菌(Psychrobacter sp.)SE6作为益生菌的体内外评价[J].海洋学报, 2012,34(2):129-135.
[24]李桂军,楼永良.脆弱拟杆菌肠毒素致病机制研究进展[J].中国人兽共患病学报, 2009, 25(1):70-72; 77.
[25]石青峰,高玲. ICU脑膜炎败血黄杆菌所致医院获得性感染的临床特征及耐药性分析[J].国际检验医学杂志, 2013, 34(1):27-28; 31.
[26]黎雪梅,李小兵,郑宗林,等.水生动物柱状黄杆菌的研究进展[J].饲料与畜牧, 2014(6):31-37.
[27]程灿灿.住院患者粪便中产NDM超级细菌调查及基因分型研究[D].广州:南方医科大学, 2014.