重组氯毒素构建细胞靶向的活性纳米颗粒（英文）
|
摘要
功能性蛋白质在纳米尺度的可控寡聚化提供了通过重组DNA技术来设计和生产改良材料和药物的可能性.氯毒素(CTX),作为一种重组的蝎毒素,由于其优先结合癌细胞的能力而引起人们的兴趣.本研究将氯毒素设计并自组装为12 nm的常规纳米颗粒,这些纳米颗粒可穿透具有和天然毒素相同受体特异性的培养细胞.这些生物相容且可生物降解的材料,表现出与同时作为载体和治疗剂的重组毒素相应的温和但仍然显著的细胞毒活性,有希望成为用于细胞靶向治疗胶质瘤的药物载体.此外,对CTX侧区域的修改可有效影响纳米颗粒的性能,说明基于CTX的构建体可通过常规基因工程来调节其多重功能性.
引文
1 Serna N,Sánchez-García L,Unzueta U,et al.Protein-based therapeutic killing for cancer therapies.Trends Biotech,2018,36:318-335
2 DeBin JA,Maggio JE,Strichartz GR.Purification and characterization of chlorotoxin,a chloride channel ligand from the venom of the scorpion.Am J Physiol-Cell Physiol,1993,264:C361-C369
3 DeBin JA,Strichartz GR.Chloride channel inhibition by the venom of the scorpion Leiurus quinquestriatus.Toxicon,1991,29:1403-1408
4 Veiseh M,Gabikian P,Bahrami SB,et al.Tumor paint:a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci.Cancer Res,2007,67:6882-6888
5 Deshane J,Garner CC,Sontheimer H.Chlorotoxin inhibits glioma cell invasion via matrix metalloproteinase-2.J Biol Chem,2003,278:4135-4144
6 Xu T,Fan Z,Li W,et al.Identification of two novel chlorotoxin derivatives CA4 and CTX-23 with chemotherapeutic and antiangiogenic potential.Sci Rep,2016,6:19799
7 Ojeda PG,Henriques ST,Pan Y,et al.Lysine to arginine mutagenesis of chlorotoxin enhances its cellular uptake.Biopolymers,2017,108:e23025
8 Mamelak AN,Jacoby DB.Targeted delivery of antitumoral therapy to glioma and other malignancies with synthetic chlorotoxin(TM-601).Expert Opin Drug Deliver,2007,4:175-186
9 Kasai T,Nakamura K,Vaidyanath A,et al.Chlorotoxin fused to IgG-Fc inhibits glioblastoma cell motility via receptor-mediated endocytosis.J Drug Deliver,2012,2012:1-10
10 Vazquez E,Mangues R,Villaverde A.Functional recruitment for drug delivery through protein-based nanotechnologies.Nanomedicine,2016,11:1333-1336
11 Rueda F,Céspedes MV,Conchillo-SoléO,et al.Bottom-up instructive quality control in the biofabrication of smart protein materials.Adv Mater,2015,27:7816-7822
12 Serna N,Céspedes MV,Sánchez-García L,et al.Peptide-based nanostructured materials with intrinsic proapoptotic activities in CXCR4+solid tumors.Adv Funct Mater,2017,27:1700919
13 Sánchez-García L,Serna N,álamo P,et al.Self-assembling toxinbased nanoparticles as self-delivered antitumoral drugs.J Control Release,2018,274:81-92
14 Díaz R,Pallarès V,Cano-Garrido O,et al.Selective CXCR4+cancer cell targeting and potent antineoplastic effect by a nanostructured version of recombinant ricin.Small,2018,14:1800665
15 Céspedes MV,Unzueta U,Avi?óA,et al.Selective depletion of metastatic stem cells as therapy for human colorectal cancer.EMBO Mol Med,2018,10:e8772
16 Unzueta U,Ferrer-Miralles N,Cedano J,et al.Non-amyloidogenic peptide tags for the regulatable self-assembling of protein-only nanoparticles.Biomaterials,2012,33:8714-8722
17 Sánchez JM,Sánchez-García L,Pesarrodona M,et al.Conformational conversion during controlled oligomerization into nonamylogenic protein nanoparticles.Biomacromolecules,2018,19:3788-3797
18 Céspedes MV,Unzueta U,Tatkiewicz W,et al.In vivo architectonic stability of fully de novo designed protein-only nanoparticles.ACS Nano,2014,8:4166-4176
19 Unzueta U,Céspedes MV,Ferrer-Miralles N,et al.Intracellular CXCR4+cell targeting with T22-empowered protein-only nanoparticles.Int J Nanomedicine,2012,7:4533-4544
20 Serna N,Céspedes MV,Saccardo P,et al.Rational engineering of single-chain polypeptides into protein-only,BBB-targeted nanoparticles.NanoMed-Nanotechnol Biol Med,2016,12:1241-1251
21 Locatelli E,Naddaka M,Uboldi C,et al.Targeted delivery of silver nanoparticles and alisertib:in vitro and in vivo synergistic effect against glioblastoma.Nanomedicine,2014,9:839-849
22 Graf N,Mokhtari TE,Papayannopoulos IA,et al.Platinum(IV)-chlorotoxin(CTX)conjugates for targeting cancer cells.J Inorg Biochem,2012,110:58-63
23 Asphahani F,Wang K,Thein M,et al.Single-cell bioelectrical impedance platform for monitoring cellular response to drug treatment.Phys Biol,2011,8:015006
24 Locatelli E,Broggi F,Ponti J,et al.Lipophilic silver nanoparticles and their polymeric entrapment into targeted-PEG-based micelles for the treatment of glioblastoma.Adv Healthcare Mater,2012,1:342-347
25 Poty S,Désogère P,Goze C,et al.New AMD3100 derivatives for CXCR4 chemokine receptor targeted molecular imaging studies:synthesis,anti-HIV-1 evaluation and binding affinities.Dalton Trans,2015,44:5004-5016
26 Richard JP,Melikov K,Vives E,et al.Cell-penetrating peptides.JBiol Chem,2003,278:585-590
27 Dardevet L,Rani D,Aziz TAE,et al.Chlorotoxin:a helpful natural scorpion peptide to diagnose glioma and fight tumor invasion.Toxins,2015,7:1079-1101
28 Allen M,Bjerke M,Edlund H,et al.Origin of the U87MG glioma cell line:Good news and bad news.Sci Transl Med,2016,8:354re3
29 Shen J,Wolfram J,Ferrari M,et al.Taking the vehicle out of drug delivery.Mater Today,2017,20:95-97
2 DeBin JA,Maggio JE,Strichartz GR.Purification and characterization of chlorotoxin,a chloride channel ligand from the venom of the scorpion.Am J Physiol-Cell Physiol,1993,264:C361-C369
3 DeBin JA,Strichartz GR.Chloride channel inhibition by the venom of the scorpion Leiurus quinquestriatus.Toxicon,1991,29:1403-1408
4 Veiseh M,Gabikian P,Bahrami SB,et al.Tumor paint:a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci.Cancer Res,2007,67:6882-6888
5 Deshane J,Garner CC,Sontheimer H.Chlorotoxin inhibits glioma cell invasion via matrix metalloproteinase-2.J Biol Chem,2003,278:4135-4144
6 Xu T,Fan Z,Li W,et al.Identification of two novel chlorotoxin derivatives CA4 and CTX-23 with chemotherapeutic and antiangiogenic potential.Sci Rep,2016,6:19799
7 Ojeda PG,Henriques ST,Pan Y,et al.Lysine to arginine mutagenesis of chlorotoxin enhances its cellular uptake.Biopolymers,2017,108:e23025
8 Mamelak AN,Jacoby DB.Targeted delivery of antitumoral therapy to glioma and other malignancies with synthetic chlorotoxin(TM-601).Expert Opin Drug Deliver,2007,4:175-186
9 Kasai T,Nakamura K,Vaidyanath A,et al.Chlorotoxin fused to IgG-Fc inhibits glioblastoma cell motility via receptor-mediated endocytosis.J Drug Deliver,2012,2012:1-10
10 Vazquez E,Mangues R,Villaverde A.Functional recruitment for drug delivery through protein-based nanotechnologies.Nanomedicine,2016,11:1333-1336
11 Rueda F,Céspedes MV,Conchillo-SoléO,et al.Bottom-up instructive quality control in the biofabrication of smart protein materials.Adv Mater,2015,27:7816-7822
12 Serna N,Céspedes MV,Sánchez-García L,et al.Peptide-based nanostructured materials with intrinsic proapoptotic activities in CXCR4+solid tumors.Adv Funct Mater,2017,27:1700919
13 Sánchez-García L,Serna N,álamo P,et al.Self-assembling toxinbased nanoparticles as self-delivered antitumoral drugs.J Control Release,2018,274:81-92
14 Díaz R,Pallarès V,Cano-Garrido O,et al.Selective CXCR4+cancer cell targeting and potent antineoplastic effect by a nanostructured version of recombinant ricin.Small,2018,14:1800665
15 Céspedes MV,Unzueta U,Avi?óA,et al.Selective depletion of metastatic stem cells as therapy for human colorectal cancer.EMBO Mol Med,2018,10:e8772
16 Unzueta U,Ferrer-Miralles N,Cedano J,et al.Non-amyloidogenic peptide tags for the regulatable self-assembling of protein-only nanoparticles.Biomaterials,2012,33:8714-8722
17 Sánchez JM,Sánchez-García L,Pesarrodona M,et al.Conformational conversion during controlled oligomerization into nonamylogenic protein nanoparticles.Biomacromolecules,2018,19:3788-3797
18 Céspedes MV,Unzueta U,Tatkiewicz W,et al.In vivo architectonic stability of fully de novo designed protein-only nanoparticles.ACS Nano,2014,8:4166-4176
19 Unzueta U,Céspedes MV,Ferrer-Miralles N,et al.Intracellular CXCR4+cell targeting with T22-empowered protein-only nanoparticles.Int J Nanomedicine,2012,7:4533-4544
20 Serna N,Céspedes MV,Saccardo P,et al.Rational engineering of single-chain polypeptides into protein-only,BBB-targeted nanoparticles.NanoMed-Nanotechnol Biol Med,2016,12:1241-1251
21 Locatelli E,Naddaka M,Uboldi C,et al.Targeted delivery of silver nanoparticles and alisertib:in vitro and in vivo synergistic effect against glioblastoma.Nanomedicine,2014,9:839-849
22 Graf N,Mokhtari TE,Papayannopoulos IA,et al.Platinum(IV)-chlorotoxin(CTX)conjugates for targeting cancer cells.J Inorg Biochem,2012,110:58-63
23 Asphahani F,Wang K,Thein M,et al.Single-cell bioelectrical impedance platform for monitoring cellular response to drug treatment.Phys Biol,2011,8:015006
24 Locatelli E,Broggi F,Ponti J,et al.Lipophilic silver nanoparticles and their polymeric entrapment into targeted-PEG-based micelles for the treatment of glioblastoma.Adv Healthcare Mater,2012,1:342-347
25 Poty S,Désogère P,Goze C,et al.New AMD3100 derivatives for CXCR4 chemokine receptor targeted molecular imaging studies:synthesis,anti-HIV-1 evaluation and binding affinities.Dalton Trans,2015,44:5004-5016
26 Richard JP,Melikov K,Vives E,et al.Cell-penetrating peptides.JBiol Chem,2003,278:585-590
27 Dardevet L,Rani D,Aziz TAE,et al.Chlorotoxin:a helpful natural scorpion peptide to diagnose glioma and fight tumor invasion.Toxins,2015,7:1079-1101
28 Allen M,Bjerke M,Edlund H,et al.Origin of the U87MG glioma cell line:Good news and bad news.Sci Transl Med,2016,8:354re3
29 Shen J,Wolfram J,Ferrari M,et al.Taking the vehicle out of drug delivery.Mater Today,2017,20:95-97