日粮纤维对吉林白鹅免疫器官指数和肠道黏膜微生物多样性的影响
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摘要
本试验旨在研究日粮纤维对吉林白鹅免疫器官指数及肠道黏膜微生物多样性的影响。选取体重均匀的5周龄的吉林白鹅80只,随机分为4个试验组,每组20只。Ⅰ和Ⅱ组以秸秆为纤维源,水平分别为5%和8%,Ⅲ和Ⅳ组以苜蓿为纤维源,水平分别为5%和8%。测定吉林白鹅免疫器官指数,并采用PCR-DGGE技术对鹅肠道黏膜定植菌进行测定。结果表明,日粮纤维水平显著的提升了胸腺指数和脾脏指数(P <0. 05),但对腔上囊无显著影响(P> 0. 05)。吉林白鹅各肠段黏膜定植微生物多样性随纤维水平的提高而提高,其中优势菌瘤胃球菌属(Ruminococcus obeum)、梭状芽孢杆菌属(Clostridium saccharoperbutylacetonicum)、戊糖片球菌(Pediococcus pentosaceus)数量增加。
The aim of the present study was to investigate the effect of dietary fiber on immune organ index,and biochemical index of blood and mucosa colonization microbial diversity of Jilin white goose. 80 of 5 weeks old Jilin white goose were selected and randomly divided into 4 groups with 20 in each test group. Fiber levels were 5% and 8% for straw and alfalfa fiber source (Ⅰ: fiber level of 5% straw; Ⅱ: fiber level of 8% straw;Ⅲ: fiber level of 5% alfalfa;Ⅳ: fiber level of 8% alfalfa). To evaluate the effects of dietary fiber on immune organ index,colonization of bacteria was determined by PCR-DGGE technology. The test results showed that:dietary fiber levels significantly increased the thymus index,spleen index,while the fiber level on the bursa of the impact was not significant. With the increase of dietary fiber levels,duodenum,jejunum,ileum,Jilin goose,cecal mucosa colonization microbial diversity increased,and the dominant bacteria Ruminococcus,Clostridium,and Pediococcus pentosaceus increased in number.
The aim of the present study was to investigate the effect of dietary fiber on immune organ index,and biochemical index of blood and mucosa colonization microbial diversity of Jilin white goose. 80 of 5 weeks old Jilin white goose were selected and randomly divided into 4 groups with 20 in each test group. Fiber levels were 5% and 8% for straw and alfalfa fiber source (Ⅰ: fiber level of 5% straw; Ⅱ: fiber level of 8% straw;Ⅲ: fiber level of 5% alfalfa;Ⅳ: fiber level of 8% alfalfa). To evaluate the effects of dietary fiber on immune organ index,colonization of bacteria was determined by PCR-DGGE technology. The test results showed that:dietary fiber levels significantly increased the thymus index,spleen index,while the fiber level on the bursa of the impact was not significant. With the increase of dietary fiber levels,duodenum,jejunum,ileum,Jilin goose,cecal mucosa colonization microbial diversity increased,and the dominant bacteria Ruminococcus,Clostridium,and Pediococcus pentosaceus increased in number.
引文
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[2]周根来,张玲.鹅日粮纤维营养的研究进展[J].饲料研究,2011(10):55-57.
[3]李彦品.饲粮粗纤维水平对仔鹅生产性能、养分利用率及盲肠微生物区系的影响[D].扬州:扬州大学,2017.
[4]Abreu MT.Toll-like receptor signalling in the intestinal epithelium:how bacterial recognition shapes intestinal function[J].Nat Rev Immunol,2010,10(2):131-144.
[5]周海柱.鹅肠道菌群对日粮纤维响应规律及其功能研究[D].长春:吉林农业大学,2016.
[6]胡敏华,胡民强.粗纤维水平对肉鹅生长及其肠道微生物的影响[J].中国家禽,2010,32(5):27-30.
[7]Kato S,Haruta S,Cui Z J,et al.Stable coexistence of five bacterial strains as a cellulose-degrading community[J].Applied&Environmental Microbiology,2005,71(11):7 099-7 116.
[8]Peng G,Moidzuki K,Wei C,et al.Effects of different pretreatment strategies on corn stalk acidogenic fermentation using a microbial consortium[J].Bioresource Technology,2011,102(16):7 526-7531.
[9]代文婷,郑楠,赵圣国,等.瘤胃壁黏附细菌的多样性及其影响因素[J].动物营养学报,2014,26(2):304-310.
[10]SADET S,MARTIN C,MEUNIER B,et al.PCR-DGGEanalysis reveals a distinct diversity in the bacterial population attached to the rumen epithelium[J].Animal,2007,1(7):939-944.
[11]罗旭.日粮纤维水平对不同生长阶段籽鹅生长性能和胃肠道菌群的影响[D].大庆:黑龙江八一农垦大学,2017.