Anticancer activity of Salvia officinalis essential oil and its principal constituents against hormone-dependent tumour cells
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摘要
Objective: To investigate the in vitro antiproliferative action of essential oil from Salvia officinalis L.(S. officinalis) grown in Sicily(Italy), and its main components on hormone-dependent cancer cell lines. Methods: S. officinalis essential oil was prepared by hydrodistillation. The actions of the S. officinalis essential oil and its three principal components(毩-thujone, 1,8-cineole and camphor) were evaluated in LNCaP cells(prostate carcinoma), MCF7 cells(breast carcinoma) and He La cells(cervical carcinoma) at various dosages and diverse time points. Cell viability and proliferation were estimated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: S. officinalis essential oil at doses of 100 μg/m L and 200 μg/m L induced a significant reduction of cell viability in MCF7, LNCa P and He La cell lines after a 48-hour incubation. The same cell lines also showed decreased cell viability when they were treated with a mixture of three major components of the essential oil, at doses of 100 μg/mL and 200 μg/mL, after a 48-hour incubation. Conclusions: These preliminary results could shed light on the formulation of new therapeutic agents with antiproliferative activity. Thus supplementary investigations are fundamental to examine the molecular mechanisms of the anticancer effects of this species of Salvia in cancer cells and to achieve confirmation of its in vivo anticancer activity.
Objective: To investigate the in vitro antiproliferative action of essential oil from Salvia officinalis L.(S. officinalis) grown in Sicily(Italy), and its main components on hormone-dependent cancer cell lines. Methods: S. officinalis essential oil was prepared by hydrodistillation. The actions of the S. officinalis essential oil and its three principal components(毩-thujone, 1,8-cineole and camphor) were evaluated in LNCaP cells(prostate carcinoma), MCF7 cells(breast carcinoma) and He La cells(cervical carcinoma) at various dosages and diverse time points. Cell viability and proliferation were estimated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: S. officinalis essential oil at doses of 100 μg/m L and 200 μg/m L induced a significant reduction of cell viability in MCF7, LNCa P and He La cell lines after a 48-hour incubation. The same cell lines also showed decreased cell viability when they were treated with a mixture of three major components of the essential oil, at doses of 100 μg/mL and 200 μg/mL, after a 48-hour incubation. Conclusions: These preliminary results could shed light on the formulation of new therapeutic agents with antiproliferative activity. Thus supplementary investigations are fundamental to examine the molecular mechanisms of the anticancer effects of this species of Salvia in cancer cells and to achieve confirmation of its in vivo anticancer activity.
Objective: To investigate the in vitro antiproliferative action of essential oil from Salvia officinalis L.(S. officinalis) grown in Sicily(Italy), and its main components on hormone-dependent cancer cell lines. Methods: S. officinalis essential oil was prepared by hydrodistillation. The actions of the S. officinalis essential oil and its three principal components(毩-thujone, 1,8-cineole and camphor) were evaluated in LNCaP cells(prostate carcinoma), MCF7 cells(breast carcinoma) and He La cells(cervical carcinoma) at various dosages and diverse time points. Cell viability and proliferation were estimated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: S. officinalis essential oil at doses of 100 μg/m L and 200 μg/m L induced a significant reduction of cell viability in MCF7, LNCa P and He La cell lines after a 48-hour incubation. The same cell lines also showed decreased cell viability when they were treated with a mixture of three major components of the essential oil, at doses of 100 μg/mL and 200 μg/mL, after a 48-hour incubation. Conclusions: These preliminary results could shed light on the formulation of new therapeutic agents with antiproliferative activity. Thus supplementary investigations are fundamental to examine the molecular mechanisms of the anticancer effects of this species of Salvia in cancer cells and to achieve confirmation of its in vivo anticancer activity.
引文
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[3]Ojima I,Lichtenthal B,Lee S,Wang C,Wang X.Taxane anticancer agents:A patent perspective.Expert Opin Ther Pat 2016;26(1):1-20.Doi:10.1517/13543776.2016.1111872.
[4]Mukherjee A,Basu S,Sarkar N,Ghosh AC.Advances in cancer therapy with plant based natural products.Curr Med Chem 2001;8(12):1467-1486.
[5]Gutiérrez-Rebolledo GA,Drier-Jonas S,Jiménez-Arellanes MA.Natural compounds and extracts from Mexican medicinal plants with antileishmaniasis activity:An update.Asian Pac J Trop Med 2017;10(12):1105-1110.Doi:10.1016/j.apjtm.2017.10.016.
[6]Aziz ZAA,Ahmad A,Setapar SHM,Karakucuk A,Azim MM,Lokhat D,et al.Essential oils:Extraction techniques,pharmaceutical and therapeutic potential-A review.Curr Drug Metab 2018;19(13):1100-1110.Doi:10.2174/1389200219666180723144850.
[7]Blowman K,Magalh?es M,Lemos MFL,Cabral C,Pires IM.Anticancer properties of essential oils and other natural products.Evid Based Complement Alternat Med 2018;2018:3149362.Doi:10.1155/2018/3149362.
[8]Pavithra PS,Mehta A,Verma RS.Induction of apoptosis by essential oil from P.missionis in skin epidermoid cancer cells.Phytomedicine 2018;50:184-195.Doi:10.1016/j.phymed.2017.11.004.
[9]Lima EJSP,Alves RG,D Elia GMA,Anuncia??o TAD,Silva VR,Santos LS,et al.Antitumor effect of the essential oil from the leaves of Croton matourensis Aubl.(Euphorbiaceae).Molecules 2018;23(11):2974.Doi:10.3390/molecules23112974.
[10]Zhong Z,Yu H,Wang S,Wang Y,Cui L.Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells.Chin Med2018;13:44.Doi:10.1186/s13020-018-0203-z.
[11]Al-Otaibi WA,Alkhatib MH,Wali AN.Cytotoxicity and apoptosis enhancement in breast and cervical cancer cells upon coadministration of mitomycin C and essential oils in nanoemulsion formulations.Biomed Pharmacother 2018;106:946-955.Doi:10.1016/j.biopha.2018.07.041.
[12]Russo A,Cardile V,Graziano ACE,Avola R,Bruno M,Rigano D.Involvement of Bax and Bcl-2 in induction of apoptosis by essential oils of three Lebanese Salvia species in human prostate cancer cells.Int J Mol Sci 2018;19(1):292.Doi:10.3390/ijms19010292.
[13]Khoury M,Stien D,Eparvier V,Ouaini N,El Beyrouthy M.Report on the medicinal use of eleven Lamiaceae species in Lebanon and rationalization of their antimicrobial potential by examination of the chemical composition and antimicrobial activity of their essential oils.Evid Based Complement Alternat Med 2016;2016:2547169.Doi:10.1155/2016/2547169.
[14]Kamatou,GP,Makunga NP,Ramogola WP,Viljoen AM.South African Salvia species:A review of biological activities and phytochemistry.JEthnopharmacol 2008;119(3):664-672.
[15]Cardile V,Russo A,Formisano C,Rigano D,Senatore F,Arnold NA,et al.Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon:Chemical composition,antimicrobial activity and inhibitory effect on human melanoma cells.J Ethnopharmacol 2009;126(2):265-272.
[16]Privitera G,Napoli E,Luca T,Ruberto G,Castorina S.In vitro antiproliferative effect of Salvia officinalis essential oil and its three main components on human lung cancer cells.Am J Phytomedicine Clin Therapeuthics 2014;2(10):1159-1168.
[17]Napoli EM,Ruberto G.Sicilian aromatic plants:From traditional heritage to a new agro-industrial exploitation.In:Kralis JF(ed).Spices:Types,uses and health benefits.New York,USA:Nova Publishers;2012,p.1-56.
[18]Khedher MRB,Khedher SB,Chaieb I,Tounsi S,Hammami M.Chemical composition and biological activities of Salvia officinalis essential oil from Tunisia.EXCLI J 2017;16:160-173.Doi:10.17179/excli2016-832.
[19]Granata G,Stracquadanio S,Leonardi M,Napoli E,Consoli G,Cafiso V,et al.Essential oils encapsulated in polymer-based nanocapsules as potential candidates for application in food preservation.Food Chem2018;269:286-292.
[20]Privitera G,Luca T,Musso N,Vancheri C,Crimi N,Barresi V,et al.In vitro antiproliferative effect of trastuzumab(Herceptin(?))combined with cetuximab(Erbitux(?))in a model of human non-small cell lung cancer expressing EGFR and HER2.Clin Exp Med 2016;16(2):161-168.
[21]ABICH S.r.l.Citotoxicity evaluation.[Online].Available from:onetruthusa.com/wp-content/uploads/2016/05/Cytotoxicity-AbichReport.pdf[Accessed on 5 February 2018].
[22]Ghoncheh M,Pournamdar Z,Salehiniya H.Incidence and mortality and epidemiology of breast cancer in the world.Asian Pac J Cancer Prev2016;17(S3):43-46.
[23]Wong MC,Goggins WB,Wang HH,Fung FD,Leung C,Wong SY,et al.Global incidence and mortality for prostate cancer:Analysis of temporal patterns and trends in 36 countries.Eur Urol 2016;70(5):862-874.Doi:10.1016/j.eururo.2016.05.043.
[24]?zkan A,Erdo?an A.A comparative evaluation of antioxidant and anticancer activity of essential oil from origanum onites(lamiaceae)and its two major phenolic components.Turk J Biol 2011;35(6):735-742.
[25]Biswas R,Mandal SK,Dutta S,Bhattacharyya SS,Boujedaini N,KhudaBukhsh AR.Thujone-rich fraction of thuja occidentalis demonstrates major anti-cancer potentials:evidences from in vitro studies on A375cells.Evid Based Complement Alternat Med 2011;2011:568148.Doi:10.1093/ecam/neq042.
[26]Zhou Y,Liu JQ,Zhou ZH,Lv XT,Chen YQ,Sun LQ,et al.Enhancement of CD3AK cell proliferation and killing ability by毩-thujone.Int Immunopharmacol 2016;30:57-61.
[27]Siveen KS,Kuttan G.Thujone inhibits lung metastasis induced by B16F-10 melanoma cells in C57BL/6 mice.Can J Physiol Pharmacol 2011;89(10):691-703.
[28]Nikoli?B,Vasilijevi?B,Miti?-?ulafi?D,Vukovi?-Ga?i?B,Kne?evi?-Vuk?evi?J.Comparative study of genotoxic,antigenotoxic and cytotoxic activities of monoterpenes camphor,eucalyptol and thujone in bacteria and mammalian cells.Chem Biol Interact Int 2015;242:263-271.
[29]Gautam N,Mantha AK,Mittal S.Essential oils and their constituents as anticancer agents:A mechanistic view.Bio Med Res Int 2014;2014:154106.Doi:10.1155/2014/154106.
[30]Waris G,Ahsan H.Reactive oxygen species:Role in the development of cancer and various chronic conditions.J Carcinog 2006;5:14.
[31]Calabrese V,Giordano J,Signorile A,Laura Ontario M,Castorina S,De Pasquale C,et al.Major pathogenic mechanisms in vascular dementia:Roles of cellular stress response and hormesis in neuroprotection.JNeurosci Res 2016;94(12):1588-1603.
[32]Sharifi-Rad J,Sureda A,Tenore GC,Daglia M,Sharifi-Rad M,Valussi M,et al.Biological activities of essential oils:From plant chemoecology to traditional healing systems.Molecules 2017;22(1):E70.Doi:10.3390/molecules22010070.
[33]Blowman K,Magalh?es M,Lemos MFL,Cabral C,Pires IM.Anticancer properties of essential oils and other natural products.Evid Based Complement Alternat Med 2018;2018:3149362.Doi:10.1155/2018/3149362.
[34]Cha,JD,Kim HY,Kim YJ.Essential oil and 1,8-cineole from Artemisia lavandulaefolia induces apoptosis in KB cells via mitochondrial stress and caspase activation.Food Sci Biotechnol 2010;19(1):185-191.
[35]Akrout A,Gonzalez LA,El Jani H,Madrid PC.Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia.Food Chem Toxicol 2011;49(2):342-347.
[36]Yu JQ,Lei JC,Zhang XQ,Yu HD,Tian DZ,Liao ZX.Anticancer,antioxidant and antimicrobial activities of the essential oil of Lycopus lucidus Turcz.var.hirtus Regel.Food Chem 2011;126(4):1593-1598.
[37]Gomes MRF,Schuh RS,Jacques ALB,Augustin OA,Bordignon SAL,Dias DO,et al.Citotoxic activity evaluation of essential oils and nanoemulsions of Drimys angustifolia and D.brasiliensis on human glioblastoma(U-138 MG)and human bladder carcinoma(T24)cell lines in vitro.Rev Bras Farmacogn 2013;23(2):259-267.
[38]Nanyonga SK,Lewu F,Opoku AR,Oyedeji.Chemical composition,antioxidant activity and cytotoxicity of the essential oils of the leaves and stem of Tarchonanthus camphoratus.Afr J Pharm Pharmaco 2013;7(7):360-367.
[2]Tamrat T,Yesudass DR.A review on anticancer activity of some plantderived compounds and their mode of action.Nat Prod Chem Res 2018;6:4.Doi:10.4172/2329-6836.1000330.
[3]Ojima I,Lichtenthal B,Lee S,Wang C,Wang X.Taxane anticancer agents:A patent perspective.Expert Opin Ther Pat 2016;26(1):1-20.Doi:10.1517/13543776.2016.1111872.
[4]Mukherjee A,Basu S,Sarkar N,Ghosh AC.Advances in cancer therapy with plant based natural products.Curr Med Chem 2001;8(12):1467-1486.
[5]Gutiérrez-Rebolledo GA,Drier-Jonas S,Jiménez-Arellanes MA.Natural compounds and extracts from Mexican medicinal plants with antileishmaniasis activity:An update.Asian Pac J Trop Med 2017;10(12):1105-1110.Doi:10.1016/j.apjtm.2017.10.016.
[6]Aziz ZAA,Ahmad A,Setapar SHM,Karakucuk A,Azim MM,Lokhat D,et al.Essential oils:Extraction techniques,pharmaceutical and therapeutic potential-A review.Curr Drug Metab 2018;19(13):1100-1110.Doi:10.2174/1389200219666180723144850.
[7]Blowman K,Magalh?es M,Lemos MFL,Cabral C,Pires IM.Anticancer properties of essential oils and other natural products.Evid Based Complement Alternat Med 2018;2018:3149362.Doi:10.1155/2018/3149362.
[8]Pavithra PS,Mehta A,Verma RS.Induction of apoptosis by essential oil from P.missionis in skin epidermoid cancer cells.Phytomedicine 2018;50:184-195.Doi:10.1016/j.phymed.2017.11.004.
[9]Lima EJSP,Alves RG,D Elia GMA,Anuncia??o TAD,Silva VR,Santos LS,et al.Antitumor effect of the essential oil from the leaves of Croton matourensis Aubl.(Euphorbiaceae).Molecules 2018;23(11):2974.Doi:10.3390/molecules23112974.
[10]Zhong Z,Yu H,Wang S,Wang Y,Cui L.Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells.Chin Med2018;13:44.Doi:10.1186/s13020-018-0203-z.
[11]Al-Otaibi WA,Alkhatib MH,Wali AN.Cytotoxicity and apoptosis enhancement in breast and cervical cancer cells upon coadministration of mitomycin C and essential oils in nanoemulsion formulations.Biomed Pharmacother 2018;106:946-955.Doi:10.1016/j.biopha.2018.07.041.
[12]Russo A,Cardile V,Graziano ACE,Avola R,Bruno M,Rigano D.Involvement of Bax and Bcl-2 in induction of apoptosis by essential oils of three Lebanese Salvia species in human prostate cancer cells.Int J Mol Sci 2018;19(1):292.Doi:10.3390/ijms19010292.
[13]Khoury M,Stien D,Eparvier V,Ouaini N,El Beyrouthy M.Report on the medicinal use of eleven Lamiaceae species in Lebanon and rationalization of their antimicrobial potential by examination of the chemical composition and antimicrobial activity of their essential oils.Evid Based Complement Alternat Med 2016;2016:2547169.Doi:10.1155/2016/2547169.
[14]Kamatou,GP,Makunga NP,Ramogola WP,Viljoen AM.South African Salvia species:A review of biological activities and phytochemistry.JEthnopharmacol 2008;119(3):664-672.
[15]Cardile V,Russo A,Formisano C,Rigano D,Senatore F,Arnold NA,et al.Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon:Chemical composition,antimicrobial activity and inhibitory effect on human melanoma cells.J Ethnopharmacol 2009;126(2):265-272.
[16]Privitera G,Napoli E,Luca T,Ruberto G,Castorina S.In vitro antiproliferative effect of Salvia officinalis essential oil and its three main components on human lung cancer cells.Am J Phytomedicine Clin Therapeuthics 2014;2(10):1159-1168.
[17]Napoli EM,Ruberto G.Sicilian aromatic plants:From traditional heritage to a new agro-industrial exploitation.In:Kralis JF(ed).Spices:Types,uses and health benefits.New York,USA:Nova Publishers;2012,p.1-56.
[18]Khedher MRB,Khedher SB,Chaieb I,Tounsi S,Hammami M.Chemical composition and biological activities of Salvia officinalis essential oil from Tunisia.EXCLI J 2017;16:160-173.Doi:10.17179/excli2016-832.
[19]Granata G,Stracquadanio S,Leonardi M,Napoli E,Consoli G,Cafiso V,et al.Essential oils encapsulated in polymer-based nanocapsules as potential candidates for application in food preservation.Food Chem2018;269:286-292.
[20]Privitera G,Luca T,Musso N,Vancheri C,Crimi N,Barresi V,et al.In vitro antiproliferative effect of trastuzumab(Herceptin(?))combined with cetuximab(Erbitux(?))in a model of human non-small cell lung cancer expressing EGFR and HER2.Clin Exp Med 2016;16(2):161-168.
[21]ABICH S.r.l.Citotoxicity evaluation.[Online].Available from:onetruthusa.com/wp-content/uploads/2016/05/Cytotoxicity-AbichReport.pdf[Accessed on 5 February 2018].
[22]Ghoncheh M,Pournamdar Z,Salehiniya H.Incidence and mortality and epidemiology of breast cancer in the world.Asian Pac J Cancer Prev2016;17(S3):43-46.
[23]Wong MC,Goggins WB,Wang HH,Fung FD,Leung C,Wong SY,et al.Global incidence and mortality for prostate cancer:Analysis of temporal patterns and trends in 36 countries.Eur Urol 2016;70(5):862-874.Doi:10.1016/j.eururo.2016.05.043.
[24]?zkan A,Erdo?an A.A comparative evaluation of antioxidant and anticancer activity of essential oil from origanum onites(lamiaceae)and its two major phenolic components.Turk J Biol 2011;35(6):735-742.
[25]Biswas R,Mandal SK,Dutta S,Bhattacharyya SS,Boujedaini N,KhudaBukhsh AR.Thujone-rich fraction of thuja occidentalis demonstrates major anti-cancer potentials:evidences from in vitro studies on A375cells.Evid Based Complement Alternat Med 2011;2011:568148.Doi:10.1093/ecam/neq042.
[26]Zhou Y,Liu JQ,Zhou ZH,Lv XT,Chen YQ,Sun LQ,et al.Enhancement of CD3AK cell proliferation and killing ability by毩-thujone.Int Immunopharmacol 2016;30:57-61.
[27]Siveen KS,Kuttan G.Thujone inhibits lung metastasis induced by B16F-10 melanoma cells in C57BL/6 mice.Can J Physiol Pharmacol 2011;89(10):691-703.
[28]Nikoli?B,Vasilijevi?B,Miti?-?ulafi?D,Vukovi?-Ga?i?B,Kne?evi?-Vuk?evi?J.Comparative study of genotoxic,antigenotoxic and cytotoxic activities of monoterpenes camphor,eucalyptol and thujone in bacteria and mammalian cells.Chem Biol Interact Int 2015;242:263-271.
[29]Gautam N,Mantha AK,Mittal S.Essential oils and their constituents as anticancer agents:A mechanistic view.Bio Med Res Int 2014;2014:154106.Doi:10.1155/2014/154106.
[30]Waris G,Ahsan H.Reactive oxygen species:Role in the development of cancer and various chronic conditions.J Carcinog 2006;5:14.
[31]Calabrese V,Giordano J,Signorile A,Laura Ontario M,Castorina S,De Pasquale C,et al.Major pathogenic mechanisms in vascular dementia:Roles of cellular stress response and hormesis in neuroprotection.JNeurosci Res 2016;94(12):1588-1603.
[32]Sharifi-Rad J,Sureda A,Tenore GC,Daglia M,Sharifi-Rad M,Valussi M,et al.Biological activities of essential oils:From plant chemoecology to traditional healing systems.Molecules 2017;22(1):E70.Doi:10.3390/molecules22010070.
[33]Blowman K,Magalh?es M,Lemos MFL,Cabral C,Pires IM.Anticancer properties of essential oils and other natural products.Evid Based Complement Alternat Med 2018;2018:3149362.Doi:10.1155/2018/3149362.
[34]Cha,JD,Kim HY,Kim YJ.Essential oil and 1,8-cineole from Artemisia lavandulaefolia induces apoptosis in KB cells via mitochondrial stress and caspase activation.Food Sci Biotechnol 2010;19(1):185-191.
[35]Akrout A,Gonzalez LA,El Jani H,Madrid PC.Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia.Food Chem Toxicol 2011;49(2):342-347.
[36]Yu JQ,Lei JC,Zhang XQ,Yu HD,Tian DZ,Liao ZX.Anticancer,antioxidant and antimicrobial activities of the essential oil of Lycopus lucidus Turcz.var.hirtus Regel.Food Chem 2011;126(4):1593-1598.
[37]Gomes MRF,Schuh RS,Jacques ALB,Augustin OA,Bordignon SAL,Dias DO,et al.Citotoxic activity evaluation of essential oils and nanoemulsions of Drimys angustifolia and D.brasiliensis on human glioblastoma(U-138 MG)and human bladder carcinoma(T24)cell lines in vitro.Rev Bras Farmacogn 2013;23(2):259-267.
[38]Nanyonga SK,Lewu F,Opoku AR,Oyedeji.Chemical composition,antioxidant activity and cytotoxicity of the essential oils of the leaves and stem of Tarchonanthus camphoratus.Afr J Pharm Pharmaco 2013;7(7):360-367.