翅果油联合有氧运动对高脂饮食小鼠血脂代谢紊乱及肝脏脂质积累的改善作用
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摘要
目的:探讨翅果油联合有氧运动对高脂饮食小鼠血脂及肝脏脂质积累的影响。方法:将雄性C57BL/6小鼠,随机分为正常对照组(NC),高脂饮食组(HF),高脂饮食+翅果油灌胃组(HS),高脂饮食+有氧运动组(HE),高脂饮食+翅果油+有氧运动组(SE),每组8只,连续干预8周;喂养结束后采用酶学试剂盒测定小鼠血清甘油三脂(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)及肝脏TC、TG含量,并计算动脉粥样硬化指数(AI)和肝脏系数;采用H&E染色观察肝脏组织病理变化。结果:翅果油、有氧运动和翅果油联合有氧运动干预组小鼠体重,血清TG、TC、LDL-C,肝脏TC、TG,肝脏系数,AI值及肝脏组织脂肪病变程度均显著低于高脂饮食组(p<0.05),且翅果油联合有氧运动干预效果更好。结论:翅果油联合有氧运动能够显著改善由高脂饮食诱导的血脂代谢紊乱和肝脏脂质积累,降低动脉粥样硬化和脂肪肝的风险。
Objective: To investigate the effect of samara oil with aerobic exercise on blood lipids and hepatic lipid accumulation in high fat diet-fed mice.Methods: The male C57 BL/6 mice were randomly divided into normal diet-fed group( NC),high fat diet-fed( HF),high fat diet-fed with samara oil group( HS),high fat diet-fed with aerobic exercise group( HE),high fat diet-fed with samara oil and aerobic exercise group( SE).Eight mice for each group were treated for 8 weeks.After experiments,ELISA assays were applied to measure the plasma triglyceride( TG),total cholesterol( TC),low-density lipoprotein cholesterol( LDL-C),how-density lipoprotein cholesterol( HDL-C),and hepatic TC,TG.Atherosclerosis index( AI) and liver coefficient were calculated,and H&E staining was used to observe histopathological changes in liver tissues.Results: The body weight,TG,TC,LDL-C of serum,TG,TC of hepatic,AI and liver tissue fatty degeneration reduced significantly compared with HF group( p < 0.05). Furthermore,the combined treatment of samara oil with aerobic exercise showed better effect.Conclusion: Samara oil with aerobic exercise could significantly improve lipid metabolism disorders and hepatic lipid accumulation induced by high fat diet feeding,and reduce the risk of atherosclerosis and fatty liver.
Objective: To investigate the effect of samara oil with aerobic exercise on blood lipids and hepatic lipid accumulation in high fat diet-fed mice.Methods: The male C57 BL/6 mice were randomly divided into normal diet-fed group( NC),high fat diet-fed( HF),high fat diet-fed with samara oil group( HS),high fat diet-fed with aerobic exercise group( HE),high fat diet-fed with samara oil and aerobic exercise group( SE).Eight mice for each group were treated for 8 weeks.After experiments,ELISA assays were applied to measure the plasma triglyceride( TG),total cholesterol( TC),low-density lipoprotein cholesterol( LDL-C),how-density lipoprotein cholesterol( HDL-C),and hepatic TC,TG.Atherosclerosis index( AI) and liver coefficient were calculated,and H&E staining was used to observe histopathological changes in liver tissues.Results: The body weight,TG,TC,LDL-C of serum,TG,TC of hepatic,AI and liver tissue fatty degeneration reduced significantly compared with HF group( p < 0.05). Furthermore,the combined treatment of samara oil with aerobic exercise showed better effect.Conclusion: Samara oil with aerobic exercise could significantly improve lipid metabolism disorders and hepatic lipid accumulation induced by high fat diet feeding,and reduce the risk of atherosclerosis and fatty liver.
引文
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[21]Zhang S,Zheng L,Dong D,et al.Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced nonalcoholic fatty liver disease in rats[J].Food Chemistry,2013,141(3):2108-2116.
[22]Jiao J,Han S F,Zhang W,et al.Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet[J].Food and Nutrition Research,2016,60:31304.
[2]Bartley GE,Yokoyama W,Young SA,et al.Hypocholesterolemic effects of hydroxypropyl methylcellulose are mediated by altered gene expression in hepatic bile and cholesterol pathways of male hamsters[J].Journal of Nutrition,2010,140(7):1255-1260.
[3]王萌萌,梅振东,张淼,等.葛根提取物对高脂血症大鼠血脂及抗氧化能力的影响[J].食品工业科技,2015,36(11):369-372.
[4]Karbasi-afshar R,Saburi A,Khedmat H.Cardiovascular disorders in the context of non-alcoholic Fatty liver disease:A literature review[J].Journal of Tehran University Heart Center,2014,9(1):1-8.
[5]陈侨侨.翅果油树籽油提取及其脂肪酸组成和易挥发成分的研究[D].太原:山西大学,2016.
[6]佚名.卫生部批准翅果油等2种新资源食品[J].中国食品,2011(5):42-42.
[7]陈雨娜.翅果油对小鼠抗氧化能力及脂质代谢的影响[J].中国油脂,2017,42(6):77-80.
[8]黄玲,刘娅,王宝贵.翅果油对老龄鼠脂质过氧化的影响[J].中华预防医学杂志,2002,36(3):215-215.
[9]白谊涵,马全福,张永青,等.翅果油胶囊对高脂血症患者血脂影响的观察[J].中华保健医学杂志,2012,14(1):46-47.
[10]Guo R,Liong EC,So KF,et al.Beneficial mechanisms of aerobic exercise on hepatic lipid metabolism in non-alcoholic fatty liver disease[J].Hepatobiliary and Pancreatic Diseases International,2015,14(2):139-144.
[11]Fernando P,Bonen A,Hoffman-Goeta L.Predicting submaximal oxygen consumption during treadmill running in mice[J].Canadian Journal of Physiology and Pharmacology,1993,71(10):854-857.
[12]Kleiner D E,Brunt E M,Van Natta M,et al.Design and validation of a histological scoring system for nonalcoholic fatty liver disease[J].Hepatology,2005,41(6):1313-1321.
[13]严煜钧.EGCG对非酒精性脂肪肝大鼠的调脂保肝作用研究[D].湖南农业大学,2014.
[14]刘金枝,陆红佳,李本娇,等.甘薯纤维素粒度对高脂大鼠的降血脂作用[J].食品工业科技,2016,37(18):289-293.
[15]Di M D,Pagano F,Magaudda L,et al.Aerobic exercise and non-alcoholic fatty liver disease:a case report[J].Journal of Sports Medicine and Physical Fitness,2009,49(4):448-452.
[16]Ogasawara J,Sakurai T,Kizaki T,et al.Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes[J].Plos One,2012,7(7):e40876.
[17]Sertie R A,Andreotti S,Proen9a AR,et al.Cessation of physical exercise changes metabolism and modifies the adipocyte cellularity of the periepididymal white adipose tissue in rats[J].Journal of Applied Physiology,2013,115(3):394-402.
[18]Sreenivasa BC,Alexander G,Kalyani B,et al.Effect of exercise and dietary modification on serum aminotransferase levels in patients with nonalcoholic steatohepatitis[J].Journal of Gastroenterology and Hepatology,2006,21(1):191-198.
[19]Yeon JE,Choi KM,Baik SH,et al.Reduced expression of peroxisome proliferator activated receptor-alpha may have an important role in the development of non-alcoholic fatty liver disease[J].Journal of Gastroenterology and Hepatology,2004,19(7):799-804.
[20]Marialena M,Johane A.Nonalcoholic steatohepatitis:The therapeutic challenge of a global epidemic[J].Current Opinion in Lipidology,2012,25(3):207-217.
[21]Zhang S,Zheng L,Dong D,et al.Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced nonalcoholic fatty liver disease in rats[J].Food Chemistry,2013,141(3):2108-2116.
[22]Jiao J,Han S F,Zhang W,et al.Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet[J].Food and Nutrition Research,2016,60:31304.