元宝枫翅果3种活性成分及抗氧化能力研究
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摘要
对元宝枫翅果的果翅、种皮、仁粕中的总酚、缩合单宁和总黄酮3种活性成分含量进行测定,并应用FRAP法、DPPH自由基清除能力和ABTS+·清除能力等方法对翅果提取物的体外抗氧化能力进行评价。结果表明,种皮中3种活性成分含量最高,其总酚、缩合单宁和总黄酮含量分别为342. 83 mgGAE/g、847. 45 mgCE/g、57. 66 mgRT/g,仁粕中含量最低;翅果不同部位提取物的FRAP抗氧化能力、对DPPH自由基和ABTS+·的清除能力均存在显著性差异,提取物的体外抗氧化能力依次表现为:种皮>果翅>仁粕;相关性分析发现,翅果不同部位提取物中总酚和缩合单宁含量与抗氧化能力呈显著正相关。
The contents of total phenolics,condensed tannins and total flavonoids in fruit wing,seed coat and seed kernel meal of Acer truncatum Bunge. were analyzed,and the vitro antioxidant capacity of the samara extract were evaluated through FRAP method,DPPH radical scavenging ability and ABTS+·scavenging capacity. The results indicated that the contents of three bioactive components in the seed coat were the highest. The contents of total phenolics,condensed tannins and total flavonoids in seed coat were 342. 83 mg GAE/g,847. 45 mgCE/g and 57. 66 mgRT/g respectively. The contents of three bioactive components in seed kernel meal were the lowest. There were significant differences in different parts of samara in the FRAP antioxidant capacity and the scavenging activities of DPPH free radical and ABTS+·,and the sequence of antioxidative capacity from high to low was: seed coat,fruit wing,seed kernel meal. In addition,the antioxidative capacity was positively correlated with the contents of total phenolics and condensed tannins in different parts of samara.
The contents of total phenolics,condensed tannins and total flavonoids in fruit wing,seed coat and seed kernel meal of Acer truncatum Bunge. were analyzed,and the vitro antioxidant capacity of the samara extract were evaluated through FRAP method,DPPH radical scavenging ability and ABTS+·scavenging capacity. The results indicated that the contents of three bioactive components in the seed coat were the highest. The contents of total phenolics,condensed tannins and total flavonoids in seed coat were 342. 83 mg GAE/g,847. 45 mgCE/g and 57. 66 mgRT/g respectively. The contents of three bioactive components in seed kernel meal were the lowest. There were significant differences in different parts of samara in the FRAP antioxidant capacity and the scavenging activities of DPPH free radical and ABTS+·,and the sequence of antioxidative capacity from high to low was: seed coat,fruit wing,seed kernel meal. In addition,the antioxidative capacity was positively correlated with the contents of total phenolics and condensed tannins in different parts of samara.
引文
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[17]BRAVO L.Polyphenols:chemistry,dietary sources,metabolism,and nutritional significances[J].Nutr Rev,1998,56(11):317-333.
[18]杨连利,李仲瑾.元宝枫种壳中单宁类型鉴定及含量测定[J].咸阳师范学院学报,2004,19(4):31-33.
[19]王慧.黄酮类化合物生物活性的研究进展[J].食品与药品,2010,12(9):347-350.
[20]张露,刘鹏飞,涂宗财,等.香榧不同部位提取物的抗氧化和酶抑制活性比较分析[EB/OL].(2017-07-13)[2018-05-23].http://kns.cnki.net/kcms/detail/11.2206.TS.20170713.1133.072.html.
[2]刘祥义,付惠,陈玉惠.元宝枫油理化特性及脂肪酸组成研究[J].中国油脂,2003,28(3):66-67.
[3]魏明,廖成华.绵阳元宝枫种仁油脂成分分析研究[J].食品工业科技,2011,32(2):127-128.
[4]王性炎,王姝清.新资源食品---元宝枫籽油[J].中国油脂,2011,36(9):56-59.
[5]樊金栓,李晓明,侯小蕊.元宝枫油抗菌作用研究[M]//王性炎.元宝枫开发利用研究.西安:陕西科学技术出版社,1996:93-96.
[6]贺浪冲,高雯.元宝枫油乳业对艾氏腹水癌小鼠的抗肿瘤作用[M]//王性炎.元宝枫开发利用研究.西安:陕西科学技术出版社,1996:77-78.
[7]赵文华,张英侠.RP-HPLC法对元宝枫果翅中3种黄酮苷元的研究[J].首都医科大学学报,2005,26(4):438-440.
[8]王性炎,孙波,梁志荣,等.元宝枫栲胶的制取、分析及应用[J].西北轻工业学院学报,1994,12(3):60-65.
[9]张玉伟,李珊珊,宋明杰,等.元宝枫种仁70%乙醇提取物的化学成分研究[J].食品科学,2017,38(24):137-143.
[10]华晓雨,陶爽,孙盛楠,等.植物次生代谢产物---酚类化合物的研究进展[J].生物技术通报,2017,33(12):22-29.
[11]BEKIR J,MARS M,SOUCHARD J P,et al.Assessment of antioxidant,anti-inflammatory,anti-cholinesterase and cytotoxic activities of pomegranate(Punica granatum)leaves[J].Food Chem Toxicol,2013,55:470-475.
[12]何肖,王文凯,马明波,等.香草醛盐酸法定量薯莨色素提取液中的缩合单宁[J].现代纺织技术,2014(6):5-8.
[13]ZHANG X X,SHI Q Q,JI D,et al.Determination of the phenolic content,profile,and antioxidant activity of seeds from nine tree peony(Paeonia section Moutan DC.)species native to China[J].Food Res Int,2017,97:141-148.
[14]ZHANG L,TU Z C,XIE X.Antihyperglycemic,antioxidant activities of two Acer palmatum cultivars,and identification of phenolics profile by UPLC-QTOF-MS/MS:new natural sources of functional constituents[J].Ind Crop Prod,2016,89:522-532.
[15]ZAOUAY F,MENA P,GARCIAVIGUERA C,et al.Antioxidant activity and physico-chemical properties of Tunisian grown pomegranate(Punica granatum L.)cultivars[J].Ind Crop Prod,2012,40:81-89.
[16]朱小芳,杨凯,赵玉红.平欧榛子活性成分含量及抗氧化性比较[J].食品科学,2017,38(19):130-136.
[17]BRAVO L.Polyphenols:chemistry,dietary sources,metabolism,and nutritional significances[J].Nutr Rev,1998,56(11):317-333.
[18]杨连利,李仲瑾.元宝枫种壳中单宁类型鉴定及含量测定[J].咸阳师范学院学报,2004,19(4):31-33.
[19]王慧.黄酮类化合物生物活性的研究进展[J].食品与药品,2010,12(9):347-350.
[20]张露,刘鹏飞,涂宗财,等.香榧不同部位提取物的抗氧化和酶抑制活性比较分析[EB/OL].(2017-07-13)[2018-05-23].http://kns.cnki.net/kcms/detail/11.2206.TS.20170713.1133.072.html.