黑龙江耐甲氧西林金黄色葡萄球菌耐药及分子分型研究
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摘要
目的了解黑龙江省健康人与屠宰场猪耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)携带情况,及其耐药和分子分型情况。方法参照黑龙江省金黄色葡萄球菌专项监测方案,对分离的金黄色葡萄球菌进行药物敏感性检测筛选MRSA,对MRSA进行spa、MLST和SCCmec基因分型,同时检测pvl基因。结果 1500份健康人样本分离出257株金黄色葡萄球菌(17.13%, 257/1500), 500份猪样本分离出149株金黄色葡萄球菌(29.8%, 149/500)。所有菌株对万古霉素、替考拉宁和利奈唑烷均敏感,对红霉素和克林霉素耐药率较高。MLST分型结果为ST9型, 1株未鉴定出型别。spa分型为t899和t2922, t899为主要型别(83%, 29/35)。SCCmec分型结果为IVb型, pvl基因均为阴性。结论黑龙江省健康人MRSA携带率比例较低,屠宰场猪携带一定程度MRSA,且猪源金黄色葡萄球菌耐药情况较重,应控制养殖动物抗生素的使用。
Objective To understand the carrying status, drug resistance and molecular typing of methicillin-resistant Staphylococcus aureus(MRSA) in healthy human and slaughterhouses pig in Heilongjiang province. Methods According to the special monitoring program of Staphylococcus aureus in Heilongjiang province, MRSA was detected by drug sensitivity test for isolated Staphylococcus aureus, and the spa, MLST and SCCmec genotyping were performed on MRSA, and pvl gene was detected at the same time. Results Totally 257 strains of Staphylococcus aureus were isolated from 1500 healthy samples(17.13%, 257/1500), and 149 strains of Staphylococcus aureus were isolated from 500 pig samples(29.8%, 149/500). All strains were sensitive to vancomycin, teicoplanin and linezolid, and had higher resistance to erythromycin and clindamycin. The typing result of MLST was ST9, and one strain was not identified. spa typing was t899 and t2922, and t899 was the main type(83%, 29/35). The results of SCCmec typing were IVb type and all pvl genes were negative. Conclusion The proportion of healthy people carrying MRSA in Heilongjiang province is low and pigs in the slaughterhouse carry a certain degree of MRSA, and the resistance of pig-derived Staphylococcus aureus is heavier, so the use of antibiotics in farmed animals should be controlled.
Objective To understand the carrying status, drug resistance and molecular typing of methicillin-resistant Staphylococcus aureus(MRSA) in healthy human and slaughterhouses pig in Heilongjiang province. Methods According to the special monitoring program of Staphylococcus aureus in Heilongjiang province, MRSA was detected by drug sensitivity test for isolated Staphylococcus aureus, and the spa, MLST and SCCmec genotyping were performed on MRSA, and pvl gene was detected at the same time. Results Totally 257 strains of Staphylococcus aureus were isolated from 1500 healthy samples(17.13%, 257/1500), and 149 strains of Staphylococcus aureus were isolated from 500 pig samples(29.8%, 149/500). All strains were sensitive to vancomycin, teicoplanin and linezolid, and had higher resistance to erythromycin and clindamycin. The typing result of MLST was ST9, and one strain was not identified. spa typing was t899 and t2922, and t899 was the main type(83%, 29/35). The results of SCCmec typing were IVb type and all pvl genes were negative. Conclusion The proportion of healthy people carrying MRSA in Heilongjiang province is low and pigs in the slaughterhouse carry a certain degree of MRSA, and the resistance of pig-derived Staphylococcus aureus is heavier, so the use of antibiotics in farmed animals should be controlled.
引文
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[14]庞璐,徐进,林兰,等.动物源性甲氧西林耐药金黄色葡萄球菌的研究进展[J].中国药事,2012,26(11):1249-1254.Pang L,Xu J,Lin L,et al.Research progress of livestock-associated methicillin resistant Staphylococcus aureus[J].Chin Pharm Aff,2012,26(11):1249-1254.
[15]万东芳,万永山,朱红军,等.皮肤软组织感染患者金黄色葡萄球菌感染状况研究[J].中华医院感染学杂志,2019,29(1):32-35.Wan DF,Wan YS,Zhu HJ,et al.Prevalence of Staphylococcus aureus infection in patients with skin and soft tissue infection and resistance to linezolid and vancomycin[J].Chin J Nosocomiol,2019,29(1):32-35.
[16]顾克菊,王雪文,钟秀君,等.社区获得性与医院获得性MRSA感染监测[J].中国感染控制杂志,2011,10(2):136-138Gu KJ,Wang XW,Zhong XJ,et al.Surveillance on infections caused by community-associated and healthcare-associated MRSA[J].Chin J Infect Contr,2011,10(2):136-138.
[17]陈庆增.Mec A基因在金黄色葡萄球菌中的分布和对耐药性的影响[D].青岛:青岛大学,2009.Chen QZ.Distribution of MecA in Staphylococcus aureus and its effect on antibiotics-resistance[D].Qingdao:Qingdao University,2009.
[2]Saravanan M,Ramachandran B,Barabadi H.The prevalence and drug resistance pattern of extended spectrum-lactamases(ESBLs)-producing Enterobacteriaceae in Africa[J].J Microbiol Pathog,2017,28(12):180-192.
[3]Yan X,Song Y,Yu X,et al.Factors associated with Staphylococcus aureus nasal carriage among healthy people in northern China[J].J Clin Microbiol Infect,2015,21:157-162.
[4]Chang SJ,Dawn MS,Jeffrey C,et al.For the vancomycin-resistant Staphylococcus aureus investigative team.infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene[J].New Eng JMed,2003,348(14):1342-1347.
[5]梁蓓蓓,王睿.社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)感染特点及治疗对策的研究进展[J].中国药物应用与监测,2011,8(6):325-329.Liang BB,Wang R.Advances in infection characteristics and treatment strategies of CA-MRSA[J].Chin J Drug Appl Monit,2011,8(6):325-329.
[6]王新,黄山,周婷,等.猪源耐甲氧西林金黄色葡萄球菌的耐药性及其SCCmec基因分型研究[J].中国预防兽医学报,2010,12(32):975-977.Wang X,Huang S,Zhou T,et al.Antimicrobial susceptibility and SCCmec genetyping of methicillin-resistant Stapylococcus aureus isolates from swine[J].Chin J Prev Veter Med,2010,12(32):975-977.
[7]CLSI M100S 26th[S].
[8]闫笑梅,陶晓霞,遇晓杰,等.健康人携带金黄色葡萄球菌耐药表型及基因型研究[J].中华流行病学杂志,2015,36(6):639-643.Yan XM,Tao XX,Yu XJ,et al.Phenotype and genotype of antimicrobial resistance on nasal Staphylococcus aureus lsolates from healthy people[J].Chin J Epidemiol,2015,36(6):639-643.
[9]Zhang K,Mcclure JA,Elsayed S,et al.Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus[J].J Clin Microbiol,2005,(43):5026-5033.
[10]Lina G,Piemont Y,Godail-Gamot F,et al.Involvement of panton-valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia[J].J.Clin Infect Dis,1999,(29):1128-1132.
[11]樊润,吴聪明,李德喜,等.河南省猪源耐甲氧西林金黄色葡萄球菌的耐药性及分子分型研究[J].中国兽医科学,2014,44(12):1223-1230.Fan R,Wu CM,Li DX,et al.Antimicrobial resistance and molecular typing of methicillin-resistant Syaphylococcus aureus from pigs in Henan province[J].Vet Sci China,2014,44(12):1223-1230.
[12]黄仆.四川地区猪群中耐甲氧西林金黄色葡萄球的分离鉴定和耐药性研究[D].雅安:四川农业大学,2014.Huang P.Antimicrobial resistance of methicillin-resistant Syaphylococcus aureus from pigs in Sichuan province[D].Yaan:Sichuan Agricultural University,2014.
[13]王雪敏,姚建楠,李蓓蓓,等.上海市猪源与人源耐甲氧西林金黄色葡萄球菌的分离及分子分型[J].中国兽医科学,2013,43(3):252-255.Wang XM,Yao JN,Li BB,et al.Isolation and molecular typing of methicillin-resistant Staphylococcus aureus from swine and human in Shanghai[J].Vet Sci China,2013,43(3):252-255.
[14]庞璐,徐进,林兰,等.动物源性甲氧西林耐药金黄色葡萄球菌的研究进展[J].中国药事,2012,26(11):1249-1254.Pang L,Xu J,Lin L,et al.Research progress of livestock-associated methicillin resistant Staphylococcus aureus[J].Chin Pharm Aff,2012,26(11):1249-1254.
[15]万东芳,万永山,朱红军,等.皮肤软组织感染患者金黄色葡萄球菌感染状况研究[J].中华医院感染学杂志,2019,29(1):32-35.Wan DF,Wan YS,Zhu HJ,et al.Prevalence of Staphylococcus aureus infection in patients with skin and soft tissue infection and resistance to linezolid and vancomycin[J].Chin J Nosocomiol,2019,29(1):32-35.
[16]顾克菊,王雪文,钟秀君,等.社区获得性与医院获得性MRSA感染监测[J].中国感染控制杂志,2011,10(2):136-138Gu KJ,Wang XW,Zhong XJ,et al.Surveillance on infections caused by community-associated and healthcare-associated MRSA[J].Chin J Infect Contr,2011,10(2):136-138.
[17]陈庆增.Mec A基因在金黄色葡萄球菌中的分布和对耐药性的影响[D].青岛:青岛大学,2009.Chen QZ.Distribution of MecA in Staphylococcus aureus and its effect on antibiotics-resistance[D].Qingdao:Qingdao University,2009.