3D-printed surface promoting osteogenic differentiation and angiogenetic factor expression of BMSCs on Ti6Al4V implants and early osseointegration in vivo

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英文篇名：3D-printed surface promoting osteogenic differentiation and angiogenetic factor expression of BMSCs on Ti6Al4V implants and early osseointegration in vivo 作者：Jinkai ; Zhang ; Wenhui ; Zhou ; Hui ; Wang ; Kaili ; Lin ; Fengshan ; Chen 英文作者：Jinkai Zhang;Wenhui Zhou;Hui Wang;Kaili Lin;Fengshan Chen;Department of Orthodontics,School & Hospital of Stomatology,Tongji University;Shanghai Engineering Research Center of Tooth Restoration and Regeneration;Department of Oral & Cranio-Maxillofacial Surgery,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology; 英文关键词：Three-dimensional printing;;Titanium alloy;;BMSCs;;Osteogenesis;;Osseointegration 中文刊名：CLKJ 英文刊名：材料科学技术(英文版) 机构：Department of Orthodontics, School & Hospital of Stomatology, Tongji University;Shanghai Engineering Research Center of Tooth Restoration and Regeneration;Department of Oral & Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology; 出版日期：2019-02-15 出版单位：Journal of Materials Science & Technology 年：2019 期：v.35 基金：support of the National Key R&D Program of China (No. 2017YFB1104100);; National Natural Science Foundation of China (No. 81371129, 81670973);; the Science and Technology Commission of Shanghai (No. 17410710500, 16DZ0503800, 17510710800);; the Fund of Shanghai Municipal Commission of Health and Family Planning (No. 201540369) 语种：英文; 页：CLKJ201902011 页数：8 CN：02 ISSN：21-1315/TG 分类号：110-117

摘要

Three-dimensional-printed(3 D-P) titanium implants display many advantages, such as design flexibility,higher efficiency, the capability to easily construct complex or customized structures, etc., and is believed to potentially replace traditional implants. However, the biological performance of the 3 D-P titanium surface has not been investigated systematically. Herein, we analyzed the surface characteristics of 3 D-P Ti6 Al4 V implants and evaluated the biological responses of bone marrow derived mesenchymal stromal cells(BMSCs) to the 3 D-P surface in vitro. Moreover, after implantation into the rat femoral condyle for3 and 6 weeks, the osseointegration performance was evaluated. The results showed the 3 D-P Ti6 Al4 V implant presented distinct fluctuant macroscale rough surface and relatively better hydrophilicity which enhanced the adhesion, proliferation, osteogenic differentiation and angiogenetic factor expression of BMSCs. Moreover, the in vivo osseointegration performance was also better than that of the control group at the early stage. The present study suggested the 3 D-P titanium alloy is a promising candidate to be used as implant material.
        Three-dimensional-printed(3 D-P) titanium implants display many advantages, such as design flexibility,higher efficiency, the capability to easily construct complex or customized structures, etc., and is believed to potentially replace traditional implants. However, the biological performance of the 3 D-P titanium surface has not been investigated systematically. Herein, we analyzed the surface characteristics of 3 D-P Ti6 Al4 V implants and evaluated the biological responses of bone marrow derived mesenchymal stromal cells(BMSCs) to the 3 D-P surface in vitro. Moreover, after implantation into the rat femoral condyle for3 and 6 weeks, the osseointegration performance was evaluated. The results showed the 3 D-P Ti6 Al4 V implant presented distinct fluctuant macroscale rough surface and relatively better hydrophilicity which enhanced the adhesion, proliferation, osteogenic differentiation and angiogenetic factor expression of BMSCs. Moreover, the in vivo osseointegration performance was also better than that of the control group at the early stage. The present study suggested the 3 D-P titanium alloy is a promising candidate to be used as implant material.

引文

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