哮喘病寒饮蕴肺证病证结合大鼠病理模型的建立与思考
|
摘要
目的:探索大鼠哮喘寒饮蕴肺证病证结合动物模型的制作,评价造模效果。方法:将39只大鼠随机分为3组:空白对照组给予常规饲养,模型组、小青龙汤组给予支气管哮喘与寒饮蕴肺证大鼠模型联合造模。结果:模型组、小青龙汤组大鼠在体征与行为学症状上不同程度上出现了病理改变,吸气阻力(Ri)、呼气阻力(Re)均增大,肺顺应性(Cdyn)降低,模型组大鼠肺组织支气管黏膜上皮细胞可见严重肿胀、增生,小青龙汤组也出现一定程度病变,但是较模型组相比有着较好的治疗效果。结论:本实验所建立的支气管哮喘寒饮蕴肺证大鼠模型,其大鼠的一般行为学表现与临床中病人所表现的症状和体征基本一致,微观指标如大鼠肺的功能、肺组织形态学等病理改变,也基本符合支气管哮喘寒饮蕴肺证的病证表现,从而在宏观及微观两层面证明了实验模型的可靠性。
Objective:The animal model of asthma combined with syndrome of cold fluid retention in lung was evaluated. Method:Thrity-nine rats were randomly divided into three groups. The control group was given conventional breeding. The model group and Xiaoqinglong Decoction group set up the model of bronchial asthma and cold fluid in the lung. Result:The model group and Xiaoqinglong Decoction group's signs and symptoms appeared pathological changes in different degrees and inspiratory resistance(Ri) and expiratory resistance(Re) were increased and lung compliance(Cdyn) decreased. The model group's lung tissue bronchial epithelial cells showed swelling and severe hyperplasia. The Xiaoqinglong Decoction group also appeared a certain degree of lesions, but compared with that of the model group there was a better therapeutic effect. Conclusion:The rats model of bronchial asthma of cold fluid in the lung was established in this experiment. The general behavior of rats are basically the same as clinical symptoms and signs of human beings. The micro indexes of lung function, lung tissue morphology, pathological changes, disease and syndrome are also consistent with bronchial asthma of cold fluid in the lung syndrome, which proves the reliability of the experimental model in macro and micro levels.
Objective:The animal model of asthma combined with syndrome of cold fluid retention in lung was evaluated. Method:Thrity-nine rats were randomly divided into three groups. The control group was given conventional breeding. The model group and Xiaoqinglong Decoction group set up the model of bronchial asthma and cold fluid in the lung. Result:The model group and Xiaoqinglong Decoction group's signs and symptoms appeared pathological changes in different degrees and inspiratory resistance(Ri) and expiratory resistance(Re) were increased and lung compliance(Cdyn) decreased. The model group's lung tissue bronchial epithelial cells showed swelling and severe hyperplasia. The Xiaoqinglong Decoction group also appeared a certain degree of lesions, but compared with that of the model group there was a better therapeutic effect. Conclusion:The rats model of bronchial asthma of cold fluid in the lung was established in this experiment. The general behavior of rats are basically the same as clinical symptoms and signs of human beings. The micro indexes of lung function, lung tissue morphology, pathological changes, disease and syndrome are also consistent with bronchial asthma of cold fluid in the lung syndrome, which proves the reliability of the experimental model in macro and micro levels.
引文
[1] 张伟荣,陈小野.中医实验学[M].北京:中国协和医科大学出版社,2004:4.
[2] 杨悦,高俪,许智慧,等.中国流行株C基因型HBV稳定复制表达小鼠模型的建立[J].解放军医学杂志,2016,41(10):793-797.
[3] 卢令慧,王景,曹愿,等.冠心病复合高血脂状态病证结合动物模型的建立与评价[J].中华中医药杂志,2016,31(5):1816-1821.
[4] 王文丽,李冬梅,施雷.咳嗽变异性哮喘肝郁肺虚病证结合动物模型的建立和评价[J].中华中医药学刊,2016,34(3):667-671.
[5] 张伯臾.中医内科学[M].上海:上海科学技术出版社,1985:59.
[6] 王洪武.慢性阻塞性肺疾病寒饮蕴肺证大鼠模型的建立[D].济南:山东中医药大学,2006.
[7] 崔正昱,李燕宁.李燕宁用苓甘五味姜辛汤治疗儿童肺寒伏饮咳嗽经验[J].时珍国医国药, 2014,25(3):730.
[8] 管新竹,张伟.咳嗽变异性哮喘与痰饮相关性探讨[J].山东中医杂志,2016(9):770-771.
[9] 张庆祥,于少泓,孙广仁,等.哮喘病寒饮蕴肺证大鼠病理模型的建立与评价[J].山东中医杂志,2006,25(2):120-122.
[10] 颜培正,孟庆岩,刘燕,等.中药剂量对哮喘病寒饮蕴肺证大鼠肺功能的影响[J].辽宁中医杂志,2016,43(4):843-845.
[11] 孙广仁,臧笑薇,高博,等.温阳化饮方对支气管哮喘病寒饮蕴肺证大鼠模型肺组织β-ARmRNA表达的影响[J].世界中西医结合杂志,2007,2(9):507-510.
[12] 李晓娟,陈家旭,刘玥芸.探讨证本质研究在完善中医辨证论治体系中的意义[J].中华中医药杂志, 2017,32(6):2353-2357.
[13] 杜武勋,朱明丹,袁宏伟,等.病证结合、方证相应的系统——系统的方证相应动态研究[J].中国中西医结合杂志,2012,32(6):839-842.
[14] 蔡子微.复杂适应系统理论—研究和认识中医学理论科学内涵的理论[J].中国病理生理杂志, 2015(10):1897.
[15] 苏芮,韩振蕴,范吉平.应用复杂巨系统思维方法探索中医脑病的研究途径[J].中医研究,2010, 23(3):5-7.
[16] 黄碧燕.关于病证结合动物模型研究现状的思考[J].中国中医药信息杂志,2010,17(1):4-6.
[17] 纪冬琛,李昌煜.病证结合动物模型的制作、评价与展望[J].浙江中医药大学学报,2010,34(4):615-618.
[18] 赵慧辉,王伟.病证结合证候模型研究基本思路[J].中华中医药杂志,2006,21(12):762-764.
[19] 刘涛,王伟.证候动物模型研究的思索[J].中华中医药杂志,2006,21(9):550-551.
[20] 康洁,高碧珍.病证结合动物模型研究概况[J].中华中医药学刊,2009,27(11):2357-2359.
[21] 富琦,陈信义.建立病证结合动物模型的新思路[J].中国中医药信息杂志,2003,10(9):79-80.
[2] 杨悦,高俪,许智慧,等.中国流行株C基因型HBV稳定复制表达小鼠模型的建立[J].解放军医学杂志,2016,41(10):793-797.
[3] 卢令慧,王景,曹愿,等.冠心病复合高血脂状态病证结合动物模型的建立与评价[J].中华中医药杂志,2016,31(5):1816-1821.
[4] 王文丽,李冬梅,施雷.咳嗽变异性哮喘肝郁肺虚病证结合动物模型的建立和评价[J].中华中医药学刊,2016,34(3):667-671.
[5] 张伯臾.中医内科学[M].上海:上海科学技术出版社,1985:59.
[6] 王洪武.慢性阻塞性肺疾病寒饮蕴肺证大鼠模型的建立[D].济南:山东中医药大学,2006.
[7] 崔正昱,李燕宁.李燕宁用苓甘五味姜辛汤治疗儿童肺寒伏饮咳嗽经验[J].时珍国医国药, 2014,25(3):730.
[8] 管新竹,张伟.咳嗽变异性哮喘与痰饮相关性探讨[J].山东中医杂志,2016(9):770-771.
[9] 张庆祥,于少泓,孙广仁,等.哮喘病寒饮蕴肺证大鼠病理模型的建立与评价[J].山东中医杂志,2006,25(2):120-122.
[10] 颜培正,孟庆岩,刘燕,等.中药剂量对哮喘病寒饮蕴肺证大鼠肺功能的影响[J].辽宁中医杂志,2016,43(4):843-845.
[11] 孙广仁,臧笑薇,高博,等.温阳化饮方对支气管哮喘病寒饮蕴肺证大鼠模型肺组织β-ARmRNA表达的影响[J].世界中西医结合杂志,2007,2(9):507-510.
[12] 李晓娟,陈家旭,刘玥芸.探讨证本质研究在完善中医辨证论治体系中的意义[J].中华中医药杂志, 2017,32(6):2353-2357.
[13] 杜武勋,朱明丹,袁宏伟,等.病证结合、方证相应的系统——系统的方证相应动态研究[J].中国中西医结合杂志,2012,32(6):839-842.
[14] 蔡子微.复杂适应系统理论—研究和认识中医学理论科学内涵的理论[J].中国病理生理杂志, 2015(10):1897.
[15] 苏芮,韩振蕴,范吉平.应用复杂巨系统思维方法探索中医脑病的研究途径[J].中医研究,2010, 23(3):5-7.
[16] 黄碧燕.关于病证结合动物模型研究现状的思考[J].中国中医药信息杂志,2010,17(1):4-6.
[17] 纪冬琛,李昌煜.病证结合动物模型的制作、评价与展望[J].浙江中医药大学学报,2010,34(4):615-618.
[18] 赵慧辉,王伟.病证结合证候模型研究基本思路[J].中华中医药杂志,2006,21(12):762-764.
[19] 刘涛,王伟.证候动物模型研究的思索[J].中华中医药杂志,2006,21(9):550-551.
[20] 康洁,高碧珍.病证结合动物模型研究概况[J].中华中医药学刊,2009,27(11):2357-2359.
[21] 富琦,陈信义.建立病证结合动物模型的新思路[J].中国中医药信息杂志,2003,10(9):79-80.