摘要
以NaCl为胁迫因子,设0(CK)、50、100、150、200、250 mmol·L~(–1) 6个盐分梯度,研究5个高羊茅(Festuca arundinacea)品种种子萌发的耐盐能力,以期筛选出耐盐能力强的高羊茅品种。结果表明,在低NaCl浓度(50 mmol·L~(–1))下,各品种发芽势、发芽率、发芽指数、种子活力指数、苗长、根长和盐害率与对照相比几乎无变化(P> 0.05)。50~250 mmol·L~(–1)的盐浓度范围内,各品种相对发芽势、相对发芽率、相对发芽指数、种子活力指数、相对苗长与相对根长均随盐浓度的增加而降低,相对盐害率随盐浓度的增加而升高。NaCl浓度达到最大(250 mmol·L~(–1))时,相对盐害率达到最高,相对发芽势、相对发芽率、相对发芽指数、种子活力指数、相对苗长、相对根长等指标降至最低。5个高羊茅品种种子萌发期耐盐性强弱依次为踏火6号>警犬>红宝石2号>贝克>超级警犬。
The top of paper(TP) germination method was used to compare the salt tolerance for five tall fescue(Festuca arundinacea) strains at the germination stage in an illumination incubator. Double-layered paper moistened with pure distilled water served as the control, whereas the papers for the experiment were moistened with 6 NaCl concentrations respectively: 50, 100, 150, 200 and 250 mmol·L–1. Germination potential, rate, and index, shoot and root length, as well as salt damage rate showed no remarkable difference between the control(P > 0.05) and the strain germinating at 50 mmol·L–1 NaCl. Relative germination potential, percentage, and index, shoot and root length, and seed vigor index decreased with increases in NaCl concentration; however, the relative salt damage increased when the NaCl stress increased from 50 to250 mmol·L–1. At 250 mmol·L–1 NaCl, the seedings were seriously damaged, and the relative germination potential,percentage, and index, shoot and root length, and seed vigor index were at their lowest(P < 0.05). The results following comprehensive assessment indicate that the 5 strains can be arranged as follows in the decreasing order of salt tolerance: Tar Heel Ⅵ > Watchdog > RubyⅡ > Baker > Super Watchdog.
引文
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