Effect of Miscanthus sinensis var.purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice
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摘要
Objective: To investigate the hair growth-promoting effect of Miscanthus sinensis var.purpurascens(MSP) flower extracton on in vitro and in vivo models.Methods: MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells(h DPCs) in vitro at the dose of 3.92–62.50 μg/m L and hair growth of C57 BL/6 mice in vivo at the dose of 1000 μg/m L.The expression of transforming growth factor β1(TGF-β1),hepatocyte growth factor(HGF),β-catenin,substance P was measured by relative quantitative realtime polymerase chain reaction.Histopathological and immunohistochemical analysis were performed.Results: MSP(7.81 μg/m L) down-regulated TGF-β1 and up-regulated HGF and β-catenin in h DPCs(P<0.01).MSP(1000 μg/m L)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase.The number of mast cells was lower in the MSP-treated mice than in other groups(P<0.05 vs.NCS group).Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control.Stem cell factor in hair follicles was up-regulated in the MSP-treated mice(P<0.01).Conclusion: The MSP flower extract may have hair growth-promotion activities.
Objective: To investigate the hair growth-promoting effect of Miscanthus sinensis var.purpurascens(MSP) flower extracton on in vitro and in vivo models.Methods: MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells(h DPCs) in vitro at the dose of 3.92–62.50 μg/m L and hair growth of C57 BL/6 mice in vivo at the dose of 1000 μg/m L.The expression of transforming growth factor β1(TGF-β1),hepatocyte growth factor(HGF),β-catenin,substance P was measured by relative quantitative realtime polymerase chain reaction.Histopathological and immunohistochemical analysis were performed.Results: MSP(7.81 μg/m L) down-regulated TGF-β1 and up-regulated HGF and β-catenin in h DPCs(P<0.01).MSP(1000 μg/m L)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase.The number of mast cells was lower in the MSP-treated mice than in other groups(P<0.05 vs.NCS group).Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control.Stem cell factor in hair follicles was up-regulated in the MSP-treated mice(P<0.01).Conclusion: The MSP flower extract may have hair growth-promotion activities.
Objective: To investigate the hair growth-promoting effect of Miscanthus sinensis var.purpurascens(MSP) flower extracton on in vitro and in vivo models.Methods: MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells(h DPCs) in vitro at the dose of 3.92–62.50 μg/m L and hair growth of C57 BL/6 mice in vivo at the dose of 1000 μg/m L.The expression of transforming growth factor β1(TGF-β1),hepatocyte growth factor(HGF),β-catenin,substance P was measured by relative quantitative realtime polymerase chain reaction.Histopathological and immunohistochemical analysis were performed.Results: MSP(7.81 μg/m L) down-regulated TGF-β1 and up-regulated HGF and β-catenin in h DPCs(P<0.01).MSP(1000 μg/m L)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase.The number of mast cells was lower in the MSP-treated mice than in other groups(P<0.05 vs.NCS group).Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control.Stem cell factor in hair follicles was up-regulated in the MSP-treated mice(P<0.01).Conclusion: The MSP flower extract may have hair growth-promotion activities.
引文
1.Botchkarev VA,Kishimoto J.Molecular control of epithelialmesenchymal interactions during hair follicle cycling.JInvestig Dermatol Symp Proc 2003;8:46-55.
2.Huelsken J,Vogel R,Erdmann B,Cotsarelis G,Birchmeier W.β-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin.Cell 2001;105:533-545.
3.Danilenko DM,Ring BD,Pierce GF.Growth factors and cytokines in hair follicle development and cycling:recent insights from animal models and the potentials for clinical therapy.Mol Med Today 1996;2:460-467.
4.Arck PC,Handjiski B,Hagen E,Joachim R,Klapp BF,Paus R.Indications for a'brain-hair follicle axis(BHA)':inhibition of keratinocyte proliferation and up-regulation of keratinocyte apoptosis in telogen hair follicles by stress and substance P.FASEB J 2001;15:2536-2538.
5.Paus R.Stress,hair growth control and the neuroendocrine immune connection.Allergo J 2000;9:411-420.
6.Ebner K,Singewald N.The role of substance P in stress and anxiety responses.Amino Acids 2006;31:251-272.
7.Suzuki H,Miura S,Liu YY,Tsuchiya M,Ishii H.Substance Pinduces degranulation of mast cells and leukocyte adhesion to venular endothelium.Peptides 1995;16:1447-1452.
8.Lee GY,Kim GS,and Cheong CH.Miscanthus sinensis var.purpurascens(Andersson)Rendle.In:Lee GY,Kim GS,Cheong CH,eds.Medicinal herbs growing in Korea.Korea Forest Seed and Variety Center,Chungbuk,Republic of Korea:Seoul Marketing Company;2013:496-497.
9.Lee JA.Identification and standardization of bioactive compounds from roots of Miscanthus sinensis var.purpurascens and Peucedanum japonicum Thunberg[dissertation].Jeju:Jeju National University;2011.(KNS ver.)
10.Kim EJ,Choi JY,Park BC,Lee BH.Platycarya strobilacea S.et Z.extract has a high antioxidant capacity and exhibits hair growth-promoting effects in male C57BL/6 mice.Prev Nutr Food Sci 2014;19:136-144.
11.Palumbo ML,Canzobre MC,Pascuan CG,Ríos H,Wald M,Genaro AM.Stress induced cognitive deficit is differentially modulated in BALB/c and C57Bl/6 mice:correlation with Th1/Th2 balance after stress exposure.J Neuroimmunol2010;218:12-20.
12.Kong MK,Kim YC.Promotion effect of black sesame oil on hair growth in an alopecia model of C57BL/6 mice.JBiomed Res 2010;11:103-116.
13.Kim EJ,Choi JY,Yu MR,Kim MY,Lee SH,Lee BH.Total polyphenols,total flavonoid contents,and antioxidant activity of Korean natural and medicinal plants.J Food Sci Technol 2012;44:337-342.
14.Naito A,Midorikawa T,Yoshino T,Ohdera M.Lipid peroxides induce early onset of catagen phase in murine hair cycles.Int J Mol Med 2008;22:725-729.
15.Hah DS,Kim CH,Kim GS,Kim ES,Kim JS.Antioxidative effects of traditional medicinal plants on lipid peroxidation.Korean J Vet Res 2005;45:341-350.
16.Seger R,Krebs EG.The MAPK signaling cascade.FASEBJ 1995;9:726-735.
17.Jindo T,Tsuboi R,Imai R,Takamori K,Rubin JS,Ogawa H.The effect of hepatocyte growth factor/scatter factor on human hair follicle growth.J Dermatol Sci 1995;10:229-232.
18.Yamauchi K,Kurosaka A.Inhibition of glycogen synthase kinase-3 enhances the expression of alkaline phosphatase and insulin-like growth factor-1 in human primary dermal papilla cell culture and maintains mouse hair bulbs in organ culture.Arch Dermatol Res 2009;301:357-365.
19.Kumamoto T,Shalhevet D,Matsue H,Mummert ME,Ward BR,Jester JV,et al.Hair follicles serve as local reservoirs of skin mast cell precursors.Blood 2003;102:1654-1660.
20.Arck PC,Handjiski B,Peters EM,Hagen E,Klapp BF,Paus R.Topical minoxidil counteracts stress-induced hair growth inhibition in mice.Exp Dermatol 2003;12:580-590.
21.Peters EM,Maurer M,Botchkarev VA,Jensen Kd,Welker P,Scott GA,et al.Kit is expressed by epithelial cells in vivo.JInvest Dermatol 2003;121:976-984.
22.Tsuji Y,Denda S,Soma T,Raftery L,Momoi T,Hibino T.Apotential suppressor of TGF-βdelays catagen progression in hair follicles.J Investig Dermatol Symp Proc 2003;8:65-68.
23.Yano K,Brown LF,Detmar M.Control of hair growth and follicle size by VEGF-mediated angiogenesis.J Clin Invest2001;107:409-417.
24.Lim EJ,Kang HJ,Jung HJ,Kim J,Lim CJ,Park EH.Antiinflammatory,anti-angiogenic and anti-nociceptive activities of 4-hydroxybenzaldehyde.Biomol Ther 2008;16:231-236.
2.Huelsken J,Vogel R,Erdmann B,Cotsarelis G,Birchmeier W.β-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin.Cell 2001;105:533-545.
3.Danilenko DM,Ring BD,Pierce GF.Growth factors and cytokines in hair follicle development and cycling:recent insights from animal models and the potentials for clinical therapy.Mol Med Today 1996;2:460-467.
4.Arck PC,Handjiski B,Hagen E,Joachim R,Klapp BF,Paus R.Indications for a'brain-hair follicle axis(BHA)':inhibition of keratinocyte proliferation and up-regulation of keratinocyte apoptosis in telogen hair follicles by stress and substance P.FASEB J 2001;15:2536-2538.
5.Paus R.Stress,hair growth control and the neuroendocrine immune connection.Allergo J 2000;9:411-420.
6.Ebner K,Singewald N.The role of substance P in stress and anxiety responses.Amino Acids 2006;31:251-272.
7.Suzuki H,Miura S,Liu YY,Tsuchiya M,Ishii H.Substance Pinduces degranulation of mast cells and leukocyte adhesion to venular endothelium.Peptides 1995;16:1447-1452.
8.Lee GY,Kim GS,and Cheong CH.Miscanthus sinensis var.purpurascens(Andersson)Rendle.In:Lee GY,Kim GS,Cheong CH,eds.Medicinal herbs growing in Korea.Korea Forest Seed and Variety Center,Chungbuk,Republic of Korea:Seoul Marketing Company;2013:496-497.
9.Lee JA.Identification and standardization of bioactive compounds from roots of Miscanthus sinensis var.purpurascens and Peucedanum japonicum Thunberg[dissertation].Jeju:Jeju National University;2011.(KNS ver.)
10.Kim EJ,Choi JY,Park BC,Lee BH.Platycarya strobilacea S.et Z.extract has a high antioxidant capacity and exhibits hair growth-promoting effects in male C57BL/6 mice.Prev Nutr Food Sci 2014;19:136-144.
11.Palumbo ML,Canzobre MC,Pascuan CG,Ríos H,Wald M,Genaro AM.Stress induced cognitive deficit is differentially modulated in BALB/c and C57Bl/6 mice:correlation with Th1/Th2 balance after stress exposure.J Neuroimmunol2010;218:12-20.
12.Kong MK,Kim YC.Promotion effect of black sesame oil on hair growth in an alopecia model of C57BL/6 mice.JBiomed Res 2010;11:103-116.
13.Kim EJ,Choi JY,Yu MR,Kim MY,Lee SH,Lee BH.Total polyphenols,total flavonoid contents,and antioxidant activity of Korean natural and medicinal plants.J Food Sci Technol 2012;44:337-342.
14.Naito A,Midorikawa T,Yoshino T,Ohdera M.Lipid peroxides induce early onset of catagen phase in murine hair cycles.Int J Mol Med 2008;22:725-729.
15.Hah DS,Kim CH,Kim GS,Kim ES,Kim JS.Antioxidative effects of traditional medicinal plants on lipid peroxidation.Korean J Vet Res 2005;45:341-350.
16.Seger R,Krebs EG.The MAPK signaling cascade.FASEBJ 1995;9:726-735.
17.Jindo T,Tsuboi R,Imai R,Takamori K,Rubin JS,Ogawa H.The effect of hepatocyte growth factor/scatter factor on human hair follicle growth.J Dermatol Sci 1995;10:229-232.
18.Yamauchi K,Kurosaka A.Inhibition of glycogen synthase kinase-3 enhances the expression of alkaline phosphatase and insulin-like growth factor-1 in human primary dermal papilla cell culture and maintains mouse hair bulbs in organ culture.Arch Dermatol Res 2009;301:357-365.
19.Kumamoto T,Shalhevet D,Matsue H,Mummert ME,Ward BR,Jester JV,et al.Hair follicles serve as local reservoirs of skin mast cell precursors.Blood 2003;102:1654-1660.
20.Arck PC,Handjiski B,Peters EM,Hagen E,Klapp BF,Paus R.Topical minoxidil counteracts stress-induced hair growth inhibition in mice.Exp Dermatol 2003;12:580-590.
21.Peters EM,Maurer M,Botchkarev VA,Jensen Kd,Welker P,Scott GA,et al.Kit is expressed by epithelial cells in vivo.JInvest Dermatol 2003;121:976-984.
22.Tsuji Y,Denda S,Soma T,Raftery L,Momoi T,Hibino T.Apotential suppressor of TGF-βdelays catagen progression in hair follicles.J Investig Dermatol Symp Proc 2003;8:65-68.
23.Yano K,Brown LF,Detmar M.Control of hair growth and follicle size by VEGF-mediated angiogenesis.J Clin Invest2001;107:409-417.
24.Lim EJ,Kang HJ,Jung HJ,Kim J,Lim CJ,Park EH.Antiinflammatory,anti-angiogenic and anti-nociceptive activities of 4-hydroxybenzaldehyde.Biomol Ther 2008;16:231-236.