四味消瘤饮抗脑胶质瘤血管生成的研究
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摘要
目的:观察四味消瘤饮对脑胶质瘤血管生成的抑制作用,探讨四味消瘤饮抑制脑胶质瘤血管生成的机制。方法:建立人脑胶质瘤细胞株U87皮下移植瘤模型,将裸鼠随机分为生理盐水组、常规剂量四味消瘤饮组、大剂量四味消瘤饮组、替莫唑胺(Temozolomide,TMZ)组、四味消瘤饮+TMZ组,测量裸鼠肿瘤体积,Weidner法计数裸鼠肿瘤组织微血管密度,酶联吸附法检测裸鼠血清血管内皮生长因子(Vascular endothelial growth factor,VEGF)、内皮抑素(Endostatin,ES)的含量。结果:与生理盐水组比较,TMZ组肿瘤体积减小(P <0.05,P <0.01);常规剂量四味消瘤饮组、大剂量四味消瘤饮组、四味消瘤饮+TMZ组肿瘤体积、组织微血管密度(Microvessel density,MVD)、血清VEGF含量减小,血清ES含量升高,差异均有统计学意义(P <0.05,P <0.01)。结论:四味消瘤饮能抑制脑胶质瘤血管生成,其机制可能是通过上调ES和下调VEGF实现的。
Objective:To observe the inhibitory effect of Siwei Xiaoliu Yin on angiogenesis in gliomas, and explore its mechanism. Methods:Established subcutaneou transplantation tumor model of U87 glioma,randomly divided nude mice into the normal saline group, the routine dose of Siwei Xiaoliu Yin group, the high dose of Siwei Xiaoliu Yin group, the temozolomide(TMZ) group,and the Siwei Xiaoliu Yin plus TMZ group,measured the tumor volume,counted the microvessel density(MVD) of tumor tissues by Weidner method,and detected the content of vascular endothelial growth factor(VEGF) and endostatin(ES) in serum of nude mice by enzyme-linked immunosorbent assay(ELISA). Results:Compared with that in the normal saline group,the tumor volume in the TMZ group was decreased(P < 0.05). The tumor volume,MVD and the content of VEGF in serum in the routine dose and the high dose of Siwei Xiaoliu Yin groups and the Siwei Xiaoliu Yin plus TMZ group were decreased,while the content of ES in the serum was increased when compared with those in the normal saline group,differences being significant(P < 0.05). Conclusion: Siwei Xiaoliu Yin can inhibit the angiogenesis in gliomas, and the mechanism is probably to up-regulate endostatin and to down-regulate vascular endothelial growth factor.
Objective:To observe the inhibitory effect of Siwei Xiaoliu Yin on angiogenesis in gliomas, and explore its mechanism. Methods:Established subcutaneou transplantation tumor model of U87 glioma,randomly divided nude mice into the normal saline group, the routine dose of Siwei Xiaoliu Yin group, the high dose of Siwei Xiaoliu Yin group, the temozolomide(TMZ) group,and the Siwei Xiaoliu Yin plus TMZ group,measured the tumor volume,counted the microvessel density(MVD) of tumor tissues by Weidner method,and detected the content of vascular endothelial growth factor(VEGF) and endostatin(ES) in serum of nude mice by enzyme-linked immunosorbent assay(ELISA). Results:Compared with that in the normal saline group,the tumor volume in the TMZ group was decreased(P < 0.05). The tumor volume,MVD and the content of VEGF in serum in the routine dose and the high dose of Siwei Xiaoliu Yin groups and the Siwei Xiaoliu Yin plus TMZ group were decreased,while the content of ES in the serum was increased when compared with those in the normal saline group,differences being significant(P < 0.05). Conclusion: Siwei Xiaoliu Yin can inhibit the angiogenesis in gliomas, and the mechanism is probably to up-regulate endostatin and to down-regulate vascular endothelial growth factor.
引文
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[3]张志强,韩富,谢海涛,等.乌骨藤对裸鼠胶质瘤作用的实验研究[J].新中医,2013,45(1):160-161.
[4]Jhaveri N,Chen TC,Hofman FM.Tumor vasculature and glioma stem cells:Contributions to glioma progression[J].Cancer Lett,2014,16(14):783-786.
[5]Borhani K,Bamdad T,Hashempour T.Low dose of lenalidomide enhances NK cell activity:possible implication as an adjuvant[J].Iranian Journal of Immunology,2017,14(2):151-158.
[6]Sun H,Guo D,Su Y,et al.Hyperplasia of pericytes is one of the main characteristics of microvascular architecture in malignant glio-ma[J].PLoS One,2014,9(12):e114246.
[7]鲁美钰,仲维兰,司春枫,等.肿瘤血管生成机制及抗肿瘤血管新生的靶向药物研究进展[J].安徽医药,2018,22(5):798-802.
[8]Bhattacharya R,Xia L,Fan F,et al.Inhibition of intracrine VEGF signaling prevents colorectal cancer cell migration and invasion[J].Cancer Res,2016,76(14):3255.
[9]Krcek R,Matschke V,Theis V,et al.Vascular endothelial growth factor,irradiation,and axitinib have diverse effects on motility and proliferation of glioblastoma multiforme cells[J].Front Oncol,2017,7(8):182.
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