消肿片中枫香脂的鉴别与松香酸的检查
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摘要
目的建立消肿片中枫香脂定性鉴别的薄层色谱(TLC)法及测定松香酸含量的高效液相-二极管阵列(HPLC-DAD)法。方法采用TLC法对枫香脂进行定性鉴别。色谱柱为Agilent ZORBAX SB-C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸溶液(18∶82),流速为1.0 mL/min,柱温为35℃,检测波长为241 nm,并同时采集200～400 nm波长的光谱图,对消肿片中是否含有松香酸进行定性、定量分析。结果 5批消肿片(5个生产厂家)有4批TLC鉴别未检出枫香脂,而在HPLC-DAD法中检出了松香酸。结论该方法准确可靠,操作简单,可用于消肿片中枫香脂的鉴别和松香酸的定性、定量分析。
Objective To establish a TLC method for identification of liquiddambaris resina and an HPLC-DAD method for content determination of abietic acid in Xiaozhong Tablets.Methods TLC method was used for qualitative identification for Liquiddambaris resina.The abietic acid in Xiaozhong Tablets was qualitatively and quantitatively analyzed with chromatographic column of Agilent ZORBAX SB-C_(18) column(250 mm × 4.6 mm,5 μm),the mobile phase of acetonitrile-0.1% formic acid(18 ∶ 82),the flow rate of 1.0 mL/min,the column temperature of 35 ℃ and the detection wavelength of 241 nm, and the spectrum at wavelength of 200-400 nm was simultaneously collected.Results Among the 5 batches of Xiaozhong Tablets(5 manufacturers),liquiddambaris resina were not identified in 4 batches,and abietic acid was detected by HPLC-DAD in the same 4 batches of Xiaozhong Tablets.Conclusion The methods are accurate,reliable and simple,which can be used for the identification of liquiddambaris resina and qualitative and quantitative analysis of abietic acid in Xiaozhong Tablets.
Objective To establish a TLC method for identification of liquiddambaris resina and an HPLC-DAD method for content determination of abietic acid in Xiaozhong Tablets.Methods TLC method was used for qualitative identification for Liquiddambaris resina.The abietic acid in Xiaozhong Tablets was qualitatively and quantitatively analyzed with chromatographic column of Agilent ZORBAX SB-C_(18) column(250 mm × 4.6 mm,5 μm),the mobile phase of acetonitrile-0.1% formic acid(18 ∶ 82),the flow rate of 1.0 mL/min,the column temperature of 35 ℃ and the detection wavelength of 241 nm, and the spectrum at wavelength of 200-400 nm was simultaneously collected.Results Among the 5 batches of Xiaozhong Tablets(5 manufacturers),liquiddambaris resina were not identified in 4 batches,and abietic acid was detected by HPLC-DAD in the same 4 batches of Xiaozhong Tablets.Conclusion The methods are accurate,reliable and simple,which can be used for the identification of liquiddambaris resina and qualitative and quantitative analysis of abietic acid in Xiaozhong Tablets.
引文
[1]中华人民共和国卫生部药典委员会.卫生部药品标准·中药成方制剂(第7册)[M].北京:人民卫生出版社,1993:154.
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[4]陈玉敏,曹红,刑俊波,等.麝香接骨胶囊中松香酸检测的研究[J].中华中医药杂志,2014,29(9):2982-2984.
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[6]汪杨丽,苏晶,张毅.舒筋活血丸中非法添加物松香酸的检测方法[J].中国现代中药,2015,17(3):244-246.
[7]武佳丽,曲范娜,张清波,等.小儿化毒系列制剂中非法添加松香酸的检测方法研究[J].中国药物警戒,2016,13(8):468-471.
[8]仇雅静.高效液相色谱法检查骨筋丸胶囊中非法添加的松香酸[J].中国药业,2011,20(4):39-40.
[9]聂俭林,刘学云,李勇成,等.消肿片质量标准研究[J].中国中医药信息杂志,2006,13(4):40-42.
[2]夏小中,张昌权.掺伪乳香、没药的鉴别[J].现代中药研究与实践,2007,21(1):28-29.
[3]钟名诚,饶伟文,肖聪.乳香的商品调查与质量检测方法研究[J].中国现代应用药学,2012,29(5):401-414.
[4]陈玉敏,曹红,刑俊波,等.麝香接骨胶囊中松香酸检测的研究[J].中华中医药杂志,2014,29(9):2982-2984.
[5]钟名诚,饶伟文,彭飞燕.小活络丸中松香酸的检测方法研究[J].中国药师,2013,16(12):1791-1794.
[6]汪杨丽,苏晶,张毅.舒筋活血丸中非法添加物松香酸的检测方法[J].中国现代中药,2015,17(3):244-246.
[7]武佳丽,曲范娜,张清波,等.小儿化毒系列制剂中非法添加松香酸的检测方法研究[J].中国药物警戒,2016,13(8):468-471.
[8]仇雅静.高效液相色谱法检查骨筋丸胶囊中非法添加的松香酸[J].中国药业,2011,20(4):39-40.
[9]聂俭林,刘学云,李勇成,等.消肿片质量标准研究[J].中国中医药信息杂志,2006,13(4):40-42.