光照对非光敏型茄子花青素合成相关基因的影响
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摘要
以‘FGM’茄子品种(非光敏型)为实验材料,应用Real-Time PCR技术,分析套袋处理对茄子果皮花青素合成的关键酶基因(SmCHS,SmDFR,SmCHI,SmF3'H,SmF3'5'H,SmANS)和转录因子(SmMYB1,SmCOP1)表达水平的影响。结果表明,与对照未套袋果皮相比,SmCHI、SmF3'H和SmANS基因的表达量在套袋处理后没有显著变化,但是SmCOP1、SmCHS、SmDFR、F3'5'H四个基因在套袋处理后表达量明显降低,而SmMYB1基因的表达量在套袋处理后表达量上升,对比分析光敏型茄子的花青素合成相关基因的表达模式,SmMYB1和SmCOP1两个基因在光敏性差异显著的两个品种中表达模式截然相反,这些基因可能与光照对花青素合成的调控作用有关。
Eggplant variety‘FGM'was used in this experiment. Several structural genes(SmCHS, SmDFR, SmCHI,SmF3'H, SmF3'5'H, and SmANS) and transcriptional factors(SmMYB1 and SmCOP1) involved in anthocyanin biosynthesis of eggplant were analyzed under bagging treatments using Real-Time PCR. The results indicated that the expression quantity of SmCHI, SmF3'H, and SmANS showed no significant changes after bagging treatments compared with control. Whereas, the expression of SmCOP1, SmCHS, SmDFR, and F3'5'H was evidently dropped, but increased for SmMYB1 compared with control. By comparing the expression patterns for anthocyanin biosynthesis related genes in light-sensitive eggplant with non-photosensitive eggplant, the results demonstrated that the expression profiles of SmMYB1 and SmCOP1 have exactly opposite effects in two distinct different photosensitive varieties. These genes may be associated with the regulation of light on anthocyanin biosynthesis in eggplant.
Eggplant variety‘FGM'was used in this experiment. Several structural genes(SmCHS, SmDFR, SmCHI,SmF3'H, SmF3'5'H, and SmANS) and transcriptional factors(SmMYB1 and SmCOP1) involved in anthocyanin biosynthesis of eggplant were analyzed under bagging treatments using Real-Time PCR. The results indicated that the expression quantity of SmCHI, SmF3'H, and SmANS showed no significant changes after bagging treatments compared with control. Whereas, the expression of SmCOP1, SmCHS, SmDFR, and F3'5'H was evidently dropped, but increased for SmMYB1 compared with control. By comparing the expression patterns for anthocyanin biosynthesis related genes in light-sensitive eggplant with non-photosensitive eggplant, the results demonstrated that the expression profiles of SmMYB1 and SmCOP1 have exactly opposite effects in two distinct different photosensitive varieties. These genes may be associated with the regulation of light on anthocyanin biosynthesis in eggplant.
引文
Aharoni A.,Vos R.,Wein M.,Sun Z.K.,Greco R.,Kroon A.Mol J.,and O'Connell A.,2001,The strawberry Fa MYB1transcription factor suppresses anthocyanin and flavonol ac cumulation in transgenic tobacco,The Plant Journal,28319-332
Ahmed N.U.,Park J.I.,Jung H.J.,Hur Y.,and Nou L.S.,2015Anthocyanin biosynthesis for cold and freezing stress toler ance and desirable color in Brassica rapa,Functional&Inte grative Genomics,15(4):383-394
Albert N.W.,Lewis D.H.,Zhang H.,Irving L.J.,Jameson P.E.and Davies K.M.,2009,Light-induced vegetative antho cyanin pigmentation in Petunia,Journal of Experimenta Botany,60(7):2191-2202
Annamraju D.S.,Yellamraju S.,and Rameshwar S.,1997,An tioxidant ability of anthocyanins against ascorbic acid oxida tion,Phytochemistry,45(4):671-674
Astell C.R.,Ahlstrom-Jonasson L.,Smith M.,Tatchell K.,Nas myth K.A.,and Hall B.D.,1981,The sequence of the DNAcoding for the mating-type loci of Saccharomyces cerevisi ae,Cell,27(1):15-23
Castellarin D.,Pfeiffer A.,Sivilotti P.,Degan M.,Peterlunger E.and Gaspero G.,2007,Transcriptional regulation of antho cyanin biosynthesis in ripening fruits of grapevine unde seasonal water deficit,Plant Cell and Environment,30(11)1381-1399
Christie P.J.,Alfenito M.R.,and Walbot V.,1994,Impact o low-temperature stress on general phenylpropanoid and an thocyanin pathways:enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings,Planta194(4):541-549
Chalker-Scott L.,1999,Environmental significance of anthocyanin in plant stress responses,Photochem.Photobiol,70:1-9
Cominelli E.,Gusmaroli G.,Allegra D.,Galbiati M.,Wade H.K.Jenkins G.I.,and Tonelli C.,2006,Expression analysis o anthocyanin regulatory genes in response to different ligh qualities in Arabidopsis thaliana,Journal of Plant Physiolo gy,165(8):886-894
Cone K.C.,Cocciolone S.M.,Moehlenkamp C.A.,Weber T.,Dru mmond B.J.,Tagliani L.A.,Bowen B.A.,and Perrot G.H.1993,Role of the regulatory gene pl in the photocontrol o maize anthocyanin pigmentation,Plant Cell,5(12):1807-1816
Cvorovic J.,Tramer F.,Granzotto M.,Candussio L.,and Decort G.,2010,Oxidative stress-based cytotoxicity of delphinidin and cyanidin in colon cancer cells,Archives of Biochem istry and Biophysics,501(1):151-157
Dooner H.K.,Robbins T.P.,and Jorgensen R.A.,1991,Genetic and developmental control of anthocyanin biosynthesis,An nual Review of Genetics,25(1):173-199
Feller A.,Machemer K.,Braun E.L.,and Grotewold E.,2011Evolutionary and comparative analysis of MYB and b HLHplant transcription factors,Plant J.,66(1):94-116
Han K.T.,Zhao L.,Tang X.J.,Hu K.,and Dai S.L.,2012,The re lationship between the expression of key genes in antho cyanin biosynthesis and the color of chrysanthemum,Yua nyi Xuebao(Acta Horticulturae Sinica),39(3):516-524(韩科厅,赵莉,唐杏姣,胡可,戴思兰,2012,菊花花青素苷合成关键基因表达与花色表型的关系,园艺学报,39(3)516-524)
Hartmann U.,Valentine W.J.,Christie J.M.,Hays J.,Jenkins G.I.and Weisshaar B.,1998,Identification of UV/blue lightre sponse elements in the Arabidopsis thaliana chalcone syn thase promoter using a homologous protoplast transient ex pression system,Plant Mol.Biol.,36(5):741-54
Jenkins G.I.,1997,UV and blue light signal transduction in Ara bidopsis,Plant Cell Environ.,20(6):773-778
Jiang M.M.,Ren L.,Lian H.L.,Liu Y.,and Chen H.Y.,2016Novel insight into the mechanism underlying light-con trolled anthocyanin accumulation in eggplant(Solanum mel ongena L.),Plant Sci.,249:46-58
Kami C.,Lorrain S.,Hornitschek P.,and Fankhauser C.,2010Light-regulated plant growth and development,Curr.Top Dev.,(91):29-66
Li Y.Y.,Mao K.,Zhao C.,Zhao X.Y.,Zhang H.L.,Shu H.R.,and Hao Y.J.,2012,Md COP1 Ubiquitin E3 ligases interact with Md MYB1 to regulate light-induced anthocyanin biosynthesi and red fruit coloration in apple,American Society of Plan Biologists,(160):1011-1022
Matus J.,Aquea F.,and Arce-Johnson P.,2008,Analysis of th grape MYB R2R3 subfamily reveals expanded wine quali ty-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes,BMC Plant Biology(8):83
Matus J.T.,Loyola R.,Vega A.,Pena-Neira A.,Bordeu E.Arce-Johnson P.,and Alcalde J.A.,2009,Post-veraison sun light exposure induces myb-mediated transcripttional regula tion of anthocyanin and flavonol synthesis in berry skins o Vitis vinifera,Journal of Experimental Botany,60(3)853-867
Meng X.C.,Xing T.,and Wang X.J.,2004,The role of light in th regulation of anthocyanin accumulation in Gerbera hybrid Plant Growth Regulation,44(3):243-250
Nesi N.,Debeaujon I.,Jond C.,Pelletier G.,Caboche M.,and Lepiniec L.,2000,The TT8 gene encodes a basic helix-lo op-helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques,Plant Cell,12:1863-1878
Niu S.S.,Xu C.J.,Zhang W.S.,Zhang B.,Li X.,Lin-Wang K.Ferguson I.B.,Allan A.C.,and Chen K.S.,2010,Coordinat ed regulation of anthocyanin biosynthesis in Chinese bay berry(Myrica rubra)fruit by a R2R3 MYB transcription fac tor,Planta,231(4):887-899
Pan L.J.,Zhang M.B.,Fan G.Q.,Chen W.T.,and Cao Y.P.,2010Cloning,sequencing and prokaryotic expression of dfr from Dendrobium,Yuanyi Xuebao(Acta Horticulturae Sinica),37(1):129-134(潘丽晶,张妙彬,范干群,陈伟庭,曹友培2010,石斛兰dfr基因的克隆,序列分析及原核表达,园艺学报,37(1):129-134)
Pérez-Díaz J.R.,Pérez-Díaz J.,Madrid-Espinoza J.,González Villanueva E.,Moreno Y.M.,and Ruiz-Lara S.,2016,New member of the R2R3-MYB transcription factors family in grapevine suppresses the anthocyanin accumulation in th flowers of transgenic tobacco,Plant Molecular Biology(90):63-76
Petroni K.,Cominelli E.,Consonni G.,Gusmaroli G.,Gavazzi G.and Tonelli C.,2000,The developmental expression of th maize regulatory gene Hopi determines germination depen dent anthocyanin accumulation,Genetics,155:323-36
Piazza P.,Procissi A.,Jenkins G.,and Tonelli C.,2002,Member of the c1/pl1 regulatory gene family mediate the response o maize aleurone and mesocotyl to different light qualities and cytokinins,Plant Physiol.,(128):1077-86
Preuss H.G.,Wallerstedt D.,Talpur N.,Tutuncuoglu S.,Echard B.,and Myers A.,2000,Effects of niacin-bound chromium and grape seed proanthocyanidin extract on the lipid profil of hypercholesterolemic subjects:a pilot study,Journal o Medicine,31(5-6):227-246
Rowan D.D.,Cao M.S.,Lin-Wang K.,Cooney J.M.,Jensen D.J.Austin P.T.,Hunt M.B.,Norling C.,Hellens R.P.,Sehaffe R.J.,and Allan A.C.,2009,Environmental regulation of lea colour in red 355,PAP1 Arabidopsis thaliana,New Phytolo gist,182(1):102-115
Seeram N.,Momin R.,Nair M.,and Bourquin L.D.,2001,Cy clooxygenase inhibitory and antioxidant cyanidin glycoside in cherries and berries,Phytomedicine,8(5):362-369
Shin D.H.,Choi M.,Kim K.,Bang G.,Cho M.,Choi S.,Choi G.and Park Y.,2013,HY5 regulates anthocyanin biosynthesi by inducing the transcriptional activation of the MYB75PAP1 transcription factor in Arabidopsis,Febs.Letters,587(10):1543-1547
Sivankalyania V.,Feygenberga O.,Diskina S.,Wright B.,and Alkan N.,2016,Increased anthocyanin and flavonoids in ma ngo fruit peel are associated with cold and pathogen resis tance,Postharvest Biology and Technology,(111):132-139
Shi S.C.,Gao Y.K.,Zhang X.H.,Sun J.Q.,Zhao L.L.,and Wang Y.,2011,Progress on plant genes involved in biosyntheti pathway of anthocyanins,Zhiwu Yanjiu(Bulletin of Botani cal Research),31(5):633-640(石少川,高亦珂,张秀海,孙佳琦,赵伶俐,王叶,2011,植物花青素生物合成相关基因的研究及应用,植物研究,31(5):633-640)
Spencer J.P.,2010,The impact of fruit flavonoids on memory and cognition,British Journal of Nutrition,104(S3):S40-S47
Springob K.,Nakajima J.,and Yamazaki M.,2003,Recent ad vances in the biosynthesis and accumulation of anthocyanins Natural Product Reports,20(3):288-303
Van Tunen A.J.,Koes R.E.,Spelt C.E.,van der Krol A.R.,Stuitj A.R.,and Mol J.N.,1988,Cloning of the two chalcone fla vanone isomerase genes from Petunia hybrida:coordinate light-regulated and differential expression of flavonoid genes,EMBO J.,7(5):1257-63
Wang Y.R.,Lu Y.F.,Hao S.X.,Zhang M.L.,Zhang J.,and Tian J.,2015,Different coloration patterns between the red-and white-fleshed fruits of malus crabapples,Scientia Horticul turae,194:26-33
Zhang Y.,Jung C.S.,and De Jong W.S.,2009,Genetic analysi of pigmented tuber flesh in potato,Theor.Appl.Genet.,19(1):143-150
Zhao Y.P.,Chen F.D.,and Guo W.M.,2003,Advances in geneti engineering of flower color of ornamental plants,Zhiwuxu Tongbao(Chinese Bulletin of Botany),20(1):51-58(赵云鹏,陈发棣,郭维明,2003,观赏植物花色基因工程研究进展,植物学通报,20(1):51-58)
Ahmed N.U.,Park J.I.,Jung H.J.,Hur Y.,and Nou L.S.,2015Anthocyanin biosynthesis for cold and freezing stress toler ance and desirable color in Brassica rapa,Functional&Inte grative Genomics,15(4):383-394
Albert N.W.,Lewis D.H.,Zhang H.,Irving L.J.,Jameson P.E.and Davies K.M.,2009,Light-induced vegetative antho cyanin pigmentation in Petunia,Journal of Experimenta Botany,60(7):2191-2202
Annamraju D.S.,Yellamraju S.,and Rameshwar S.,1997,An tioxidant ability of anthocyanins against ascorbic acid oxida tion,Phytochemistry,45(4):671-674
Astell C.R.,Ahlstrom-Jonasson L.,Smith M.,Tatchell K.,Nas myth K.A.,and Hall B.D.,1981,The sequence of the DNAcoding for the mating-type loci of Saccharomyces cerevisi ae,Cell,27(1):15-23
Castellarin D.,Pfeiffer A.,Sivilotti P.,Degan M.,Peterlunger E.and Gaspero G.,2007,Transcriptional regulation of antho cyanin biosynthesis in ripening fruits of grapevine unde seasonal water deficit,Plant Cell and Environment,30(11)1381-1399
Christie P.J.,Alfenito M.R.,and Walbot V.,1994,Impact o low-temperature stress on general phenylpropanoid and an thocyanin pathways:enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings,Planta194(4):541-549
Chalker-Scott L.,1999,Environmental significance of anthocyanin in plant stress responses,Photochem.Photobiol,70:1-9
Cominelli E.,Gusmaroli G.,Allegra D.,Galbiati M.,Wade H.K.Jenkins G.I.,and Tonelli C.,2006,Expression analysis o anthocyanin regulatory genes in response to different ligh qualities in Arabidopsis thaliana,Journal of Plant Physiolo gy,165(8):886-894
Cone K.C.,Cocciolone S.M.,Moehlenkamp C.A.,Weber T.,Dru mmond B.J.,Tagliani L.A.,Bowen B.A.,and Perrot G.H.1993,Role of the regulatory gene pl in the photocontrol o maize anthocyanin pigmentation,Plant Cell,5(12):1807-1816
Cvorovic J.,Tramer F.,Granzotto M.,Candussio L.,and Decort G.,2010,Oxidative stress-based cytotoxicity of delphinidin and cyanidin in colon cancer cells,Archives of Biochem istry and Biophysics,501(1):151-157
Dooner H.K.,Robbins T.P.,and Jorgensen R.A.,1991,Genetic and developmental control of anthocyanin biosynthesis,An nual Review of Genetics,25(1):173-199
Feller A.,Machemer K.,Braun E.L.,and Grotewold E.,2011Evolutionary and comparative analysis of MYB and b HLHplant transcription factors,Plant J.,66(1):94-116
Han K.T.,Zhao L.,Tang X.J.,Hu K.,and Dai S.L.,2012,The re lationship between the expression of key genes in antho cyanin biosynthesis and the color of chrysanthemum,Yua nyi Xuebao(Acta Horticulturae Sinica),39(3):516-524(韩科厅,赵莉,唐杏姣,胡可,戴思兰,2012,菊花花青素苷合成关键基因表达与花色表型的关系,园艺学报,39(3)516-524)
Hartmann U.,Valentine W.J.,Christie J.M.,Hays J.,Jenkins G.I.and Weisshaar B.,1998,Identification of UV/blue lightre sponse elements in the Arabidopsis thaliana chalcone syn thase promoter using a homologous protoplast transient ex pression system,Plant Mol.Biol.,36(5):741-54
Jenkins G.I.,1997,UV and blue light signal transduction in Ara bidopsis,Plant Cell Environ.,20(6):773-778
Jiang M.M.,Ren L.,Lian H.L.,Liu Y.,and Chen H.Y.,2016Novel insight into the mechanism underlying light-con trolled anthocyanin accumulation in eggplant(Solanum mel ongena L.),Plant Sci.,249:46-58
Kami C.,Lorrain S.,Hornitschek P.,and Fankhauser C.,2010Light-regulated plant growth and development,Curr.Top Dev.,(91):29-66
Li Y.Y.,Mao K.,Zhao C.,Zhao X.Y.,Zhang H.L.,Shu H.R.,and Hao Y.J.,2012,Md COP1 Ubiquitin E3 ligases interact with Md MYB1 to regulate light-induced anthocyanin biosynthesi and red fruit coloration in apple,American Society of Plan Biologists,(160):1011-1022
Matus J.,Aquea F.,and Arce-Johnson P.,2008,Analysis of th grape MYB R2R3 subfamily reveals expanded wine quali ty-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes,BMC Plant Biology(8):83
Matus J.T.,Loyola R.,Vega A.,Pena-Neira A.,Bordeu E.Arce-Johnson P.,and Alcalde J.A.,2009,Post-veraison sun light exposure induces myb-mediated transcripttional regula tion of anthocyanin and flavonol synthesis in berry skins o Vitis vinifera,Journal of Experimental Botany,60(3)853-867
Meng X.C.,Xing T.,and Wang X.J.,2004,The role of light in th regulation of anthocyanin accumulation in Gerbera hybrid Plant Growth Regulation,44(3):243-250
Nesi N.,Debeaujon I.,Jond C.,Pelletier G.,Caboche M.,and Lepiniec L.,2000,The TT8 gene encodes a basic helix-lo op-helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques,Plant Cell,12:1863-1878
Niu S.S.,Xu C.J.,Zhang W.S.,Zhang B.,Li X.,Lin-Wang K.Ferguson I.B.,Allan A.C.,and Chen K.S.,2010,Coordinat ed regulation of anthocyanin biosynthesis in Chinese bay berry(Myrica rubra)fruit by a R2R3 MYB transcription fac tor,Planta,231(4):887-899
Pan L.J.,Zhang M.B.,Fan G.Q.,Chen W.T.,and Cao Y.P.,2010Cloning,sequencing and prokaryotic expression of dfr from Dendrobium,Yuanyi Xuebao(Acta Horticulturae Sinica),37(1):129-134(潘丽晶,张妙彬,范干群,陈伟庭,曹友培2010,石斛兰dfr基因的克隆,序列分析及原核表达,园艺学报,37(1):129-134)
Pérez-Díaz J.R.,Pérez-Díaz J.,Madrid-Espinoza J.,González Villanueva E.,Moreno Y.M.,and Ruiz-Lara S.,2016,New member of the R2R3-MYB transcription factors family in grapevine suppresses the anthocyanin accumulation in th flowers of transgenic tobacco,Plant Molecular Biology(90):63-76
Petroni K.,Cominelli E.,Consonni G.,Gusmaroli G.,Gavazzi G.and Tonelli C.,2000,The developmental expression of th maize regulatory gene Hopi determines germination depen dent anthocyanin accumulation,Genetics,155:323-36
Piazza P.,Procissi A.,Jenkins G.,and Tonelli C.,2002,Member of the c1/pl1 regulatory gene family mediate the response o maize aleurone and mesocotyl to different light qualities and cytokinins,Plant Physiol.,(128):1077-86
Preuss H.G.,Wallerstedt D.,Talpur N.,Tutuncuoglu S.,Echard B.,and Myers A.,2000,Effects of niacin-bound chromium and grape seed proanthocyanidin extract on the lipid profil of hypercholesterolemic subjects:a pilot study,Journal o Medicine,31(5-6):227-246
Rowan D.D.,Cao M.S.,Lin-Wang K.,Cooney J.M.,Jensen D.J.Austin P.T.,Hunt M.B.,Norling C.,Hellens R.P.,Sehaffe R.J.,and Allan A.C.,2009,Environmental regulation of lea colour in red 355,PAP1 Arabidopsis thaliana,New Phytolo gist,182(1):102-115
Seeram N.,Momin R.,Nair M.,and Bourquin L.D.,2001,Cy clooxygenase inhibitory and antioxidant cyanidin glycoside in cherries and berries,Phytomedicine,8(5):362-369
Shin D.H.,Choi M.,Kim K.,Bang G.,Cho M.,Choi S.,Choi G.and Park Y.,2013,HY5 regulates anthocyanin biosynthesi by inducing the transcriptional activation of the MYB75PAP1 transcription factor in Arabidopsis,Febs.Letters,587(10):1543-1547
Sivankalyania V.,Feygenberga O.,Diskina S.,Wright B.,and Alkan N.,2016,Increased anthocyanin and flavonoids in ma ngo fruit peel are associated with cold and pathogen resis tance,Postharvest Biology and Technology,(111):132-139
Shi S.C.,Gao Y.K.,Zhang X.H.,Sun J.Q.,Zhao L.L.,and Wang Y.,2011,Progress on plant genes involved in biosyntheti pathway of anthocyanins,Zhiwu Yanjiu(Bulletin of Botani cal Research),31(5):633-640(石少川,高亦珂,张秀海,孙佳琦,赵伶俐,王叶,2011,植物花青素生物合成相关基因的研究及应用,植物研究,31(5):633-640)
Spencer J.P.,2010,The impact of fruit flavonoids on memory and cognition,British Journal of Nutrition,104(S3):S40-S47
Springob K.,Nakajima J.,and Yamazaki M.,2003,Recent ad vances in the biosynthesis and accumulation of anthocyanins Natural Product Reports,20(3):288-303
Van Tunen A.J.,Koes R.E.,Spelt C.E.,van der Krol A.R.,Stuitj A.R.,and Mol J.N.,1988,Cloning of the two chalcone fla vanone isomerase genes from Petunia hybrida:coordinate light-regulated and differential expression of flavonoid genes,EMBO J.,7(5):1257-63
Wang Y.R.,Lu Y.F.,Hao S.X.,Zhang M.L.,Zhang J.,and Tian J.,2015,Different coloration patterns between the red-and white-fleshed fruits of malus crabapples,Scientia Horticul turae,194:26-33
Zhang Y.,Jung C.S.,and De Jong W.S.,2009,Genetic analysi of pigmented tuber flesh in potato,Theor.Appl.Genet.,19(1):143-150
Zhao Y.P.,Chen F.D.,and Guo W.M.,2003,Advances in geneti engineering of flower color of ornamental plants,Zhiwuxu Tongbao(Chinese Bulletin of Botany),20(1):51-58(赵云鹏,陈发棣,郭维明,2003,观赏植物花色基因工程研究进展,植物学通报,20(1):51-58)