儿童患者咽拭子肺炎支原体-DNA对肺炎支原体肺炎的诊断价值
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摘要
目的探讨儿童患者咽拭子肺炎支原体(MP)-DNA对肺炎支原体肺炎(MPP)的早期诊断价值。方法采用实时荧光定量PCR对2016年3月至2018年2月期间就诊于南京医科大学第二附属医院1031例门诊患儿咽拭子标本进行MP-DNA的定量检测。结果 1031例门诊患儿MP-DNA总阳性率33.37%(344/1031);男女患儿MP-DNA阳性率的差异无统计学意义(c2=0.467,P>0.05);学龄组患儿阳性率最高42.19%(27/64),婴儿组阳性率27.91%(55/197)最低,幼儿组阳性率33.53%(115/343)、学龄前组阳性率34.43%(147/427);冬季患儿阳性率最高41.18%(168/408)、春季阳性率21.16%(40/189)最低,夏季阳性率27.02%(40/148)、秋季阳性率33.57%(96/286)。结论南京地区MP-DNA阳性率逐年上升、且随着年龄上升阳性率逐渐增高、冬季患儿阳性率最高,实时荧光定量MP-DNA具有快速、简便、敏感性高、特异性强的特点,是目前临床上早期诊断肺炎支原体感染的有效方法。
Objective To investigate the early diagnostic value of Mycoplasma pneumoniae(MP)-DNA in children with pneumonia mycoplasma pneumonia(MPP). Methods Real-time quantitative PCR was used to detect the MP-DNA of 1031 outpatient throat swabs from the Second Affiliated Hospital of Nanjing Medical University from March 2016 to February 2018. Results The total positive rate of MP-DNA in 1031 outpatients was 33.37%(344/1031). There was no significant difference in the positive rate of MP-DNA between male and female children(c2=0.467, P>0.05). The positive rate of children in school age group The highest rate was 42.19%(27/64),the positive rate of infant group was 27.91%(55/197), the positive rate of children group was 33.53%(115/343), the positive rate of preschool group was 34.43%(147/427); children in winter The positive rate was 41.18%(168/408), the spring positive rate was 21.16%(40/189), the summer positive rate was 27.02%(40/148), and the autumn positive rate was 33.57%(96/286). Conclusion The positive rate of MP-DNA in Nanjing is increasing year by year, and the positive rate increases with age, and the positive rate of children in winter is the highest. Real-time quantitative MP-DNA has the characteristics of fast, simple, high sensitivity and specificity. Tt is an effective method for early diagnosis of Mycoplasma pneumoniae infection in the clinic.
Objective To investigate the early diagnostic value of Mycoplasma pneumoniae(MP)-DNA in children with pneumonia mycoplasma pneumonia(MPP). Methods Real-time quantitative PCR was used to detect the MP-DNA of 1031 outpatient throat swabs from the Second Affiliated Hospital of Nanjing Medical University from March 2016 to February 2018. Results The total positive rate of MP-DNA in 1031 outpatients was 33.37%(344/1031). There was no significant difference in the positive rate of MP-DNA between male and female children(c2=0.467, P>0.05). The positive rate of children in school age group The highest rate was 42.19%(27/64),the positive rate of infant group was 27.91%(55/197), the positive rate of children group was 33.53%(115/343), the positive rate of preschool group was 34.43%(147/427); children in winter The positive rate was 41.18%(168/408), the spring positive rate was 21.16%(40/189), the summer positive rate was 27.02%(40/148), and the autumn positive rate was 33.57%(96/286). Conclusion The positive rate of MP-DNA in Nanjing is increasing year by year, and the positive rate increases with age, and the positive rate of children in winter is the highest. Real-time quantitative MP-DNA has the characteristics of fast, simple, high sensitivity and specificity. Tt is an effective method for early diagnosis of Mycoplasma pneumoniae infection in the clinic.
引文
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[9]崔娟,王佳,姚慧生,等.2006-2010年儿童肺炎支原体感染流行病学分析[J].中国实用儿科杂志,2013,28(6):446-448.
[10]李锐成,阎林,邹菊贤,等.961例呼吸道感染患儿MP-DNA检测结果分析[J].国际检验医学杂志,2013,34(20):2675-2677.
[2]郭美玲,王月训,郭丽.小儿肺炎支原体肺炎与链球菌属感染肺炎的CT鉴别诊断[J].中华医院感染学杂志,2015,25(2):443-445.
[3]张华,黄盛文,罗振元,等.贵州地区呼吸道感染儿童中肺炎支原体检测与分析[J].中华医院感染学杂志,2011,21(10):2135-2136.
[4]付晓燕,辛德莉,秦选光.儿童肺炎支原体感染流行病学,临床特点,发病机制及治疗研究进展[J].山东医药,2015,55(4):96-99.
[5]吕泰霞,赵水娣.肺炎支原体液体培养假阳性原因分析及解决方法探讨[J].实验与检验医学杂志,2009,27(3):323-324.
[6]曹波,郁飞,张宪伟,等.3种检测肺炎支原体感染方法的比较分析[J].东南大学学报:医学版,2016,35(5):770-772.
[7]Fend R,Geddes R,Lesellier.Use of an electronic nose to diagnose Mycobacterium boris infection in badgers and cattle[J].J Clin Microbiol,2005,43(4):1745-1751.
[8]刘志,刘义庆,田文君,等.1207例急性呼吸道疾病肺炎支原体核酸检测情况分析[J].中国卫生检验杂志,2017,27(22):3250-3252.
[9]崔娟,王佳,姚慧生,等.2006-2010年儿童肺炎支原体感染流行病学分析[J].中国实用儿科杂志,2013,28(6):446-448.
[10]李锐成,阎林,邹菊贤,等.961例呼吸道感染患儿MP-DNA检测结果分析[J].国际检验医学杂志,2013,34(20):2675-2677.