淫羊藿化学成分分析及其药代动力学研究进展
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摘要
淫羊藿作为补肾阳中药,具有悠久的用药历史。通过回顾淫羊藿化学成分分析及其药代动力学研究,发现淫羊藿成分复杂多样(如黄酮类、生物碱类、酚酸类等),易受到品种、产地、部位、加工炮制等因素的影响,且淫羊藿各类成分的体内过程差异较大,疾病、组方配伍、炮制加工及中西药联用对其药代动力学参数均有不同程度的影响。文章归纳淫羊藿成分分析及体内过程的研究思路和方法,探讨了目前研究中存在的一些问题,以期为淫羊藿质量控制、新药开发与临床应用研究提供参考。
Epimedium herb,as a traditional herb medicine for tonifying Kidney Yang,had a long history of use in Chinese medicine. Our paper had reviewed the relevant literature of chemical analysis and pharmacokinetics of Epimedium. It was found that Epimedium contained many active constituents(such as flavonoids,alkaloids,phenolic acids and so on),and its active constituents were differently affected by many factors such as cultivars,place of production,medicinal site,and processing. The bioavailability of various components of Epimedium in vivo was different,and the pharmacokinetics was differently affected by many factors such as disease,prescription compatibility,processing,and drug interaction. According to the summary of quantitative analysis of active constituents in Epimedium and the pharmacokinetic properties,some problems existed in the present research had been discussed to provide a reference for the quality control,drug development and clinical application of Epimedium herb.
Epimedium herb,as a traditional herb medicine for tonifying Kidney Yang,had a long history of use in Chinese medicine. Our paper had reviewed the relevant literature of chemical analysis and pharmacokinetics of Epimedium. It was found that Epimedium contained many active constituents(such as flavonoids,alkaloids,phenolic acids and so on),and its active constituents were differently affected by many factors such as cultivars,place of production,medicinal site,and processing. The bioavailability of various components of Epimedium in vivo was different,and the pharmacokinetics was differently affected by many factors such as disease,prescription compatibility,processing,and drug interaction. According to the summary of quantitative analysis of active constituents in Epimedium and the pharmacokinetic properties,some problems existed in the present research had been discussed to provide a reference for the quality control,drug development and clinical application of Epimedium herb.
引文
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[29]金晓勇,贾晓斌,孙娥,等.炙淫羊藿炮制过程中5种黄酮类成分变化规律研究[J].中国中药杂志,2009,34(21):2738-2742.
[30]陈彦,贾晓斌,范晨怡,等.不同品种淫羊藿生品与炮制品中5种黄酮类成分的含量比较[J].中华中医药杂志,2009,24(5):565-567.
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[33]Sun X,Qi L,Jie Z,et al. The reason leading to the increase of icariin in Herba Epimedii by heating process[J]. Journal of Pharmaceutical&Biomedical Analysis,2018,149:525-531.
[34]Zhang HM,Wang H,Wei J,et al. Comparison of the active compositions between raw and processed epimedium from different species[J].Molecules,2018,23:1656-1662.
[35]Wang NN,Huang XW,Wang XP,et al. Pipette tip solidphase extraction and high-performance liquid chromatography for the determination of flavonoids from Epimedii herba in rat serum and application of the technique to pharmacokinetic studies[J].Journal of Chromatography B,2015,990:64-72.
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[37]张锡玮,陈颖萍,夏素霞. LC-MS/MS法检测健康人血浆淫羊藿苷及药动学探索研究[J].辽宁中医杂志,2014,41(1):133-135.
[38]刘晓燕,王本杰,魏春敏,等.淫羊藿苷单体及淫羊藿总黄酮提取物大鼠药动学特征比较[J].中国医院药学杂志,2011,31(22):1858-1863.
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[40]刘聪燕,高霞,陈彦,等.淫羊藿总黄酮在骨质疏松大鼠体内的药动学行为[J].中成药,2017,39(8):1595-1600.
[41]周一帆,胡昌江,张雪洁,等.淫羊藿炮制前后在大鼠体内的药代动力学研究[J].中成药,2013,35(12):2717-2720.
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[43]许舜军,朱雅玲,余捷婧,等.喘可治注射液中朝藿定A,朝藿定B,朝藿定C和淫羊藿苷在大鼠体内的药物动力学研究[J].中国中药杂志,2016,41(1):129-133.
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[2]孟宁,孔凯,李师翁.淫羊藿属植物化学成分及药理活性研究进展[J].西北植物学,2010,30(5):1063-1073.
[3]陈旭凤,喻澜,夏秦.淫羊藿苷促前成骨细胞MC3T3-E1成骨分化的实验研究[J].中国中西医结合杂志,2018:38(6):687-692.
[4]赵锦,朱雯.淫羊藿苷对IgA肾病大鼠纤维化和炎症反应的调控作用[J].免疫学杂志,2018,34(5):385-392.
[5]夏世金,沈自尹,俞卓伟,等.基于基因表达谱研究淫羊藿总黄酮干预老年大鼠海马炎性衰老的效果与机制[J].实用老年医学,2010,24(1):24-27.
[6]朱翠香,彭玲玲,潘宇政,等.淫羊藿对气管切开插管留置大鼠免疫功能的影响[J].广西医学,2018,40(7):803-807.
[7]Wang GQ,Li DD,Huang C,et al. Icariin reduces dopaminergic neuronal loss and Microglia-Mediated inflammation in vivo and in vitro[J]. Front Mol Neurosci,2017,10:441.
[8]Chi A,Shen Z,Zhu W,et al. Characterization of a proteinbound polysaccharide from Herba Epimedii and its metabolic mechanism in chronic fatigue syndrome[J]. J Ethnopharmacol,2017,203:241-251.
[9]Shou D,Zhang Y,Shen L,et al. Flavonoids of herba epimedii enhances bone repair in a rabbit model of chronic osteomyelitis during post-infection treatment and stimulates osteoblast proliferation in vitro[J]. Phytother Res,2017,31(2):330-339.
[10]Cho JH,Jung JY,Lee BJ,et al. Epimedii herba:A promising herbal medicine for neuroplasticity[J].Phytother Res,2017,31(6):838-848.
[11]袁航,曹树萍,陈抒云,等.淫羊藿的化学成分及质量控制研究进展[J].中草药,2014,45(24):3630-3640.
[12]Jiang J,Song J,Jia XB. Phytochemistry and ethnopharmacology of Epimedium L. species[J].Chinese Herbal Medicines,2015,7(3):204-222.
[13]李遇伯,孟繁浩,鹿秀梅,等.淫羊霍化学成分的研究[J].中国中药杂志,2005,30(8):586.
[14]郭青,吴晓燕,宁青,等.淫羊藿茎叶中5种黄酮类成分分析及质量评价[J].中草药,2011,42(10):2028-2032.
[15]Chen XJ,Ji H,Zhang QW,et al. A rapid method for simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and ultra-performance liquid chromatography[J].Journal of Pharmaceutical and Biomedical Analysis,2008,46(2):226-235.
[16]冯伟红,李春,张锴镔,等.“一测多评”模式在淫羊藿药材、饮片及含淫羊藿中成药黄酮类成分检测中的一体化研究[J].中国中药杂志,2016,41(15):2843-2854.
[17]吴文辉,冯健,耿媛媛,等. 14个产地淫羊藿中淫羊藿苷含量测定[J].亚太传统医药,2014(18):8-9.
[18]王世杰,高云佳,周书繁,等.高效液相色谱法测定淫羊藿药材及饮片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的含量[J].中国医院用药评价与分析,2018,18(2):156-159.
[19]牛晓静,鲁静,段晓颖,等.基于聚类分析的淫羊藿黄酮类成分的质量分析研究[J].中华中医药杂志,2016,31(6):2386-2389.
[20]陈春雪,马元春,刘焱文,等. UPLC法同时测定淫羊藿中5种黄酮类成分的含量[J].湖北中医药大学学报,2017,19(4):40-45.
[21]黄弥娜,周燕妮,柳强,等. HPLC法测定不同产地淫羊藿中7种主要黄酮类成分的含量[J].第二军医大学学报,2015,36(12):1352-1355.
[22]高言明,陈惠玲,魏升华,等.毛细管区带电泳法测定几个不同种的淫羊藿中绿原酸的含量[J].中国药学杂志,2006,24(7):1903-1905.
[23]秦伟瀚,刘翔,张军,等.不同产地15个品种淫羊藿的质量评价[J].中成药,2017,39(9):1889-1895.
[24]高敏,刘京晶,孙欣光,等.中药淫羊藿主要资源种类木兰花碱含量的研究[J].中国中药杂志,2011,36(1):16-18.
[25]张萍,孔维军,鄢丹,等.基于淫羊藿苷测定及HPLC指纹图谱分析的淫羊藿药材质量差异评价[J].中草药,2010,41(11):1901-1904.
[26]陈翠萍,沙明,杨松松,等.朝鲜淫羊藿中木兰碱的定量研究[J].中国中药杂志,1996,21(11):41-42.
[27]周涛,张小波,郭兰萍,等.粗毛淫羊藿不同部位、不同生境中淫羊藿苷、总黄酮含量的变化分析[J].中国中药杂志,2012,37(13):1917-1921
[28]罗堃,王元清,张智敏,等.箭叶淫羊藿不同部位中淫羊藿苷及总黄酮含量测定[J].亚太传统医药,2016,12(8):24-26.
[29]金晓勇,贾晓斌,孙娥,等.炙淫羊藿炮制过程中5种黄酮类成分变化规律研究[J].中国中药杂志,2009,34(21):2738-2742.
[30]陈彦,贾晓斌,范晨怡,等.不同品种淫羊藿生品与炮制品中5种黄酮类成分的含量比较[J].中华中医药杂志,2009,24(5):565-567.
[31]曾雯雯,王晶,贾凌云,等.不同干燥和炮制方法对朝鲜淫羊藿中黄酮类化合物的影响[J].沈阳药科大学学报,2011,28(7):559-563.
[32]谢娟平,向纪明,王浩东.不同方法炮制对巫山淫羊藿中主要成分朝藿定C和淫羊藿苷的影响[J].药物分析杂志,2011,31(4):674-677.
[33]Sun X,Qi L,Jie Z,et al. The reason leading to the increase of icariin in Herba Epimedii by heating process[J]. Journal of Pharmaceutical&Biomedical Analysis,2018,149:525-531.
[34]Zhang HM,Wang H,Wei J,et al. Comparison of the active compositions between raw and processed epimedium from different species[J].Molecules,2018,23:1656-1662.
[35]Wang NN,Huang XW,Wang XP,et al. Pipette tip solidphase extraction and high-performance liquid chromatography for the determination of flavonoids from Epimedii herba in rat serum and application of the technique to pharmacokinetic studies[J].Journal of Chromatography B,2015,990:64-72.
[36]Sun M,Yin Y,Wei J,et al. Development and validation of a HPLC-MS/MS method for simultaneous determination of twelve bioactive compounds in epimedium:application to a pharmacokinetic study in rats[J]. Molecules,2018,23(6):1322-1325.
[37]张锡玮,陈颖萍,夏素霞. LC-MS/MS法检测健康人血浆淫羊藿苷及药动学探索研究[J].辽宁中医杂志,2014,41(1):133-135.
[38]刘晓燕,王本杰,魏春敏,等.淫羊藿苷单体及淫羊藿总黄酮提取物大鼠药动学特征比较[J].中国医院药学杂志,2011,31(22):1858-1863.
[39]Wu CS,Zhang JL,Zhou TH,et al. Simultaneous determination of seven flavonoids in dog plasma by ultra-performance liquid chromatography–tandem mass spectrometry and its application to a bioequivalence study of bioactive components in Herba Epimedii and Er-Xian Decoction[J].Journal of Pharmaceutical and Biomedical Analysis,2011,54(1):186-191.
[40]刘聪燕,高霞,陈彦,等.淫羊藿总黄酮在骨质疏松大鼠体内的药动学行为[J].中成药,2017,39(8):1595-1600.
[41]周一帆,胡昌江,张雪洁,等.淫羊藿炮制前后在大鼠体内的药代动力学研究[J].中成药,2013,35(12):2717-2720.
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