LncRNA MEG3对口腔鳞状细胞癌细胞增殖、侵袭及凋亡的影响
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摘要
目的探究LncRNA MEG3在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织和细胞中的表达水平及对OSCC细胞增殖、侵袭和凋亡的影响。方法 qRT-PCR法检测OSCC患者口腔粘膜组织、肿瘤组织及Tca8113细胞系中LncRNA MEG3的表达水平。构建pcDNA3.1-MEG3重组质粒转染Tca8113细胞,分为3组:转染过表达pcDNA3.1-MEG3的质粒组(MEG3组)、空质粒组pcDNA3.1-NC (NC组)和空白对照组(BLANK组)。分别采用qRT-PCR法、CCK-8法、流式细胞术和Transwell检测LncRNA MEG3表达、细胞增殖、凋亡率和侵袭能力。结果与正常口腔黏膜组织和细胞相比,LncRNA MEG3在OSCC组织和细胞中的表达下调(P<0.05)。体外实验发现,与空白对照组和NC组相比,过表达LncRNA MEG3组细胞增殖和侵袭能力受到抑制,凋亡率明显升高(P<0.05)。结论 LncRNA MEG3在OSCC组织和细胞中低表达;LncRNA MEG3过表达可抑制OCSS细胞的增殖和侵袭,促进凋亡。
Objective To investigate the expression of LncRNA MEG3 in the tissues and cells of oral squamous cell carcinoma(OSCC)and its effect on the proliferation,invasion and apoptosis of OSCC cells.Methods The expression of LncRNA MEG3 in the oral mucosa,tumor tissues and Tca8113 cell line of OSCC patients was detected by qRT-PCR.The recombinant plasmid pcDNA3.1-MEG3 was constructed to transfect the Tca8113 cells.The Tca8113 cells transfected with overexpressed pcDNA3.1-MEG3 were divided into the MEG3 group,those transfected with pcDNA3.1-NC were divided into the NC group,and those without any transfection were divided into the blank control group.The expression,cell proliferation,apoptosis rate and invasion ability of LncRNA MEG3 were detected by the methods of qRT-PCR,CCK-8,flow cytometry and Transwell,respectively.Results The expression of LncRNA MEG3 was significantly down-regulated in the OSCC tissues and Tca8113 cells when compared with the normal oral mucosa tissues and cells(P<0.05).The results of vitro experiments showed that the proliferation and invasion ability of the MEG3 group was significantly inhibited and the apoptosis rate was significantly increased when compared with the blank control group and the NC group(P<0.05).Conclusion The expression of LncRNA MEG3 is low in the OSCC tissues and Tca8113 cells,and its overexpression could inhibit the proliferation and invasion of OCSS cells and promote their apoptosis.
Objective To investigate the expression of LncRNA MEG3 in the tissues and cells of oral squamous cell carcinoma(OSCC)and its effect on the proliferation,invasion and apoptosis of OSCC cells.Methods The expression of LncRNA MEG3 in the oral mucosa,tumor tissues and Tca8113 cell line of OSCC patients was detected by qRT-PCR.The recombinant plasmid pcDNA3.1-MEG3 was constructed to transfect the Tca8113 cells.The Tca8113 cells transfected with overexpressed pcDNA3.1-MEG3 were divided into the MEG3 group,those transfected with pcDNA3.1-NC were divided into the NC group,and those without any transfection were divided into the blank control group.The expression,cell proliferation,apoptosis rate and invasion ability of LncRNA MEG3 were detected by the methods of qRT-PCR,CCK-8,flow cytometry and Transwell,respectively.Results The expression of LncRNA MEG3 was significantly down-regulated in the OSCC tissues and Tca8113 cells when compared with the normal oral mucosa tissues and cells(P<0.05).The results of vitro experiments showed that the proliferation and invasion ability of the MEG3 group was significantly inhibited and the apoptosis rate was significantly increased when compared with the blank control group and the NC group(P<0.05).Conclusion The expression of LncRNA MEG3 is low in the OSCC tissues and Tca8113 cells,and its overexpression could inhibit the proliferation and invasion of OCSS cells and promote their apoptosis.
引文
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[14]Soudyab M,Iranpour M,Ghafouri-Fard S.The role of long non-coding RNAs in breast cancer[J].Arch Iran Med,2016,19(7):508-517.
[15]Gao W,Chan J Y,Wong T S.Long non-coding RNAderegulation in tongue squamous cell carcinoma[J].Biomed Res Int,2014,2014:405860.
[16]刘珂,侯毅,郑稼.LncRNA MEG3对骨肉瘤细胞增殖、侵袭和凋亡的影响[J].郑州大学学报(医学版),2018,53(1):56-59.
[17]Zhou X Y,Ji G P,Ke X Q,et al.MiR-141inhibits gastric cancer proliferation by interacting with long noncoding RNAMEG3and down-regulating E2F3expression[J].Dig Dis Sci,2015,60(11):3271-3282.
[18]周静,卓倩,张静云,等.母源性印记基因MEG3在肿瘤信号通路中的研究进展[J].药物生物技术,2018,25(4):359-362.
[2]Xie S,Xu H,Shan X F,et al.Clinicopathological and prognostic significance of survivin expression in patients with oral squamous cell carcinoma:evidence from a meta-analysis[J].PLoS One,2015,10(2):e0116517.
[3]Lee C F,Chiang N N,Lu Y H,et al.Benzyl isothiocyanate(BITC)triggers mitochondria-mediated apoptotic machinery in human cisplatin-resistant oral cancer CAR cells[J].Biomedicine(Taipei),2018,8(3):15.
[4]Li Y S,Chen H,Pan T,et al.LncRNA ontology:inferring lncRNA functions based on chromatin states and expression patterns[J].Oncotarget,2015,6(37):39793-39805.
[5]Iguchi T,Uchi R,Nambara S,et al.A long noncoding RNA,lncRNA-ATB,is involved in the progression and prognosis of colorectal cancer[J].Anticancer Res,2015,35(3):1385-1388.
[6]Tang H K,Wu Z Y,Zhang J P,et al.Salivary lncRNA as a potential marker for oral squamous cell carcinoma diagnosis[J].Mol Med Rep,2013,7(3):761-766.
[7]Miyoshi N,Wagatsuma H,Wakana S,et al.Identification of an imprinted gene,Meg3/Gtl2and its human homologue MEG3,first mapped on mouse distal chromosome 12 and human chromosome 14q[J].Genes Cells,2000,5(3):211-220.
[8]Wang P,Chen D,Ma H B,et al.LncRNA MEG3enhances cisplatin sensitivity in non-small cell lung cancer by regulating miR-21-5p/SOX7axis[J].Onco Targets Ther,2017,10:5137-5149.
[9]Liu Z X,Wu C,Xie N N,et al.Long non-coding RNAMEG3 inhibits the proliferation and metastasis of oral squamous cell carcinoma by regulating the WNT/β-catenin signaling pathway[J].Oncol Lett,2017,14(4):4053-4058.
[10]詹艳萍,张勇,张泽林.LncRNA MEG3对卵巢上皮癌细胞自噬的调控作用[J].热带医学杂志,2018,18(8):1056-1059.
[11]Gejman R,Batista D L,Zhong Y,et al.Selective loss of MEG3 expression and intergenic differentially methylated region hypermethylation in the MEG3/DLK1locus in human clinically nonfunctioning pituitary adenomas[J].J Clin Endocrinol Metab,2008,93(10):4119-4125.
[12]Jia L F,Wei S B,Gan Y H,et al.Expression,regulation and roles of miR-26aand MEG3in tongue squamous cell carcinoma[J].Int J Cancer,2014,135(10):2282-2293.
[13]王丽萍,陈伟鸿,葛林虎.长链非编码RNA在口腔鳞状细胞癌的研究进展[J].全科口腔医学电子杂志,2018,5(19):16-18.
[14]Soudyab M,Iranpour M,Ghafouri-Fard S.The role of long non-coding RNAs in breast cancer[J].Arch Iran Med,2016,19(7):508-517.
[15]Gao W,Chan J Y,Wong T S.Long non-coding RNAderegulation in tongue squamous cell carcinoma[J].Biomed Res Int,2014,2014:405860.
[16]刘珂,侯毅,郑稼.LncRNA MEG3对骨肉瘤细胞增殖、侵袭和凋亡的影响[J].郑州大学学报(医学版),2018,53(1):56-59.
[17]Zhou X Y,Ji G P,Ke X Q,et al.MiR-141inhibits gastric cancer proliferation by interacting with long noncoding RNAMEG3and down-regulating E2F3expression[J].Dig Dis Sci,2015,60(11):3271-3282.
[18]周静,卓倩,张静云,等.母源性印记基因MEG3在肿瘤信号通路中的研究进展[J].药物生物技术,2018,25(4):359-362.