柚皮苷对骨肉瘤细胞生物学功能的影响
|
摘要
目的:探讨柚皮苷对骨肉瘤细胞生物学功能的调节作用。方法:将骨肉瘤MG63细胞分成三组,分别用NaCl(对照组)、10μmol/ml和20μmol/ml浓度的柚皮苷作用于细胞,通过MTT实验观察不同时间不同浓度的柚皮苷对MG63细胞增殖的作用。流式细胞术分析10μmol/ml和20μmol/ml柚皮苷对MG63细胞细胞周期的影响。通过Western blot检测不同浓度柚皮苷对增殖相关蛋白(Cyclin D1)的调节作用。Transwell实验观察10μmol/ml和20μmol/ml柚皮苷对MG63细胞迁移能力的影响。通过Western blot检测不同浓度柚皮苷对侵袭转移相关蛋白(MMP2)的调节作用。结果:MTT检测结果发现10μmol/ml和20μmol/ml浓度的柚皮苷作用MG63细胞24 h后,对MG63细胞增殖的抑制率分别为(24.10±0.03)%和(46.94±0.03)%。流式细胞术结果发现柚皮苷可以对MG63细胞G_1/S期的转导造成阻滞。Western blot结果指出,柚皮苷可以抑制Cyclin D1的表达。Transwell实验发现柚皮苷作用后可以显著抑制MG63细胞的迁移。Western blot检测发现,柚皮苷可以抑制MG63细胞中MMP2的表达。结论:柚皮苷可以抑制骨肉瘤细胞的增殖和迁移。
Objective:To investigate the effect of naringin on the biological function of osteosarcoma.Methods:MG63 osteosarcoma cells were divided into three groups.The cells were treated with NaCl(control group),10 μmol/ml and 20 μmol/ml naringin respectively.The effect of naringin on the proliferation of MG63 cells at different concentrations at different time was observed by MTT.Flow cytometry was used to analyze the effects of 10 μmol/ml and 20 μmol/ml naringin on the cell cycle of MG63 cells.The regulation of naringin with different concentrations on proliferation related protein(Cyclin D1) was detected by Western blot.The effect of naringin on the migration of MG63 cells was observed by Transwell.The regulation of naringin with different concentrations on the metastasis related protein(MMP2) was detected by Western blot.Results:MTT assay showed that the inhibitory rates of 10 μmol/ml and 20 μmol/ml naringin on MG63 cells after 24 h were(24.10±0.03)% and(46.94±0.03)%.Flow cytometry results showed that naringin could block the G_1/S phase transduction of MG63 cells.The results of Western blot indicated that naringin could inhibit the expression of Cyclin D1.Transwell assay found that the action of naringin could significantly inhibit the migration of MG63 cells.Western blot detection showed that naringin could inhibit the expression of MMP2 in MG63 cells.Conclusion:Naringin can inhibit the proliferation and migration of osteosarcoma cells.
Objective:To investigate the effect of naringin on the biological function of osteosarcoma.Methods:MG63 osteosarcoma cells were divided into three groups.The cells were treated with NaCl(control group),10 μmol/ml and 20 μmol/ml naringin respectively.The effect of naringin on the proliferation of MG63 cells at different concentrations at different time was observed by MTT.Flow cytometry was used to analyze the effects of 10 μmol/ml and 20 μmol/ml naringin on the cell cycle of MG63 cells.The regulation of naringin with different concentrations on proliferation related protein(Cyclin D1) was detected by Western blot.The effect of naringin on the migration of MG63 cells was observed by Transwell.The regulation of naringin with different concentrations on the metastasis related protein(MMP2) was detected by Western blot.Results:MTT assay showed that the inhibitory rates of 10 μmol/ml and 20 μmol/ml naringin on MG63 cells after 24 h were(24.10±0.03)% and(46.94±0.03)%.Flow cytometry results showed that naringin could block the G_1/S phase transduction of MG63 cells.The results of Western blot indicated that naringin could inhibit the expression of Cyclin D1.Transwell assay found that the action of naringin could significantly inhibit the migration of MG63 cells.Western blot detection showed that naringin could inhibit the expression of MMP2 in MG63 cells.Conclusion:Naringin can inhibit the proliferation and migration of osteosarcoma cells.
引文
[1] Li C,Xu B,Miu X,et al.Inhibition of miRNA-21 attenuates the proliferation and metastasis of human osteosarcoma by upregulating PTEN[J].Experimental and Therapeutic Medicine,2018(15):1036-1040.
[2] Andersen GB,Knudsen A,Hager H,et al.miRNA profiling identifies deregulated miRNAs associated with osteosarcoma development and time to metastasis in two large cohorts[J].Molecular Oncology,2018(12):114-131.
[3] He Y,Ma J,Wang A,et al.A support vector machine and a random forest classifier indicates a 15-miRNA set related to osteosarcoma recurrence[J].OncoTargets and Therapy,2018(11):253-269.
[4] Song YZ,Li JF.Circular RNA hsa_circ_0001564 regulates osteosarcoma proliferation and apoptosis by acting miRNA sponge[J].Biochemical and Biophysical Research Communications,2018(495):2369-2375.
[5] Zhu H,Gao J,Wang L,et al.In vitro study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor-kappaB signaling pathway[J].Experimental and Therapeutic Medicine,2018(15):2643-2648.
[6] Li H,Yang B,Huang J,et al.Naringin inhibits growth potential of human triple-negative breast cancer cells by targeting beta-catenin signaling pathway[J].Toxicology Letters,2013(220):219-228.
[7] Ramesh E,Alshatwi AA.Naringin induces death receptor and mitochondria-mediated apoptosis in human cervical cancer (SiHa) cells[J].Food and Chemical Toxicology,2013(51):97-105.
[8] Raha S,Yumnam S,Hong GE,et al.Naringin induces autophagy-mediated growth inhibition by downregulating the PI3K/Akt/mTOR cascade via activation of MAPK pathways in AGS cancer cells[J].International Journal of Oncology,2015(47):1061-1069.
[9] Li T,Xiao Y,Huang T.HIF1alpha induced upregulation of lncRNA UCA1 promotes cell growth in osteosarcoma by inactivating the PTEN/AKT signaling pathway[J].Oncology Reports,2018(39):1072-1080.
[10] Zhao Z,Jia Q,Wu MS,et al.Degalactotigonin,a natural compound from solanum nigrum L,inhibits growth and metastasis of osteosarcoma through GSK3beta inactivation-mediated repression of the Hedgehog/Gli1 pathway[J].Clinical Cancer Research,2018(24):130-144.
[11] Yoshinaga A,Kajiya N,Oishi K,et al.NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation[J].European Journal of Pharmacology,2016(782):21-29.
[12] Zeng L,Zhen Y,Chen Y,et al.Naringin inhibits growth and induces apoptosis by a mechanism dependent on reduced activation of NFkappaB/COX2caspase-1 pathway in HeLa cervical cancer cells[J].International Journal of Oncology,2014(45):1929-1936.
[13] Camargo CA,Gomes-Marcondes MC,Wutzki NC,et al.Naringin inhibits tumor growth and reduces interleukin-6 and tumor necrosis factor alpha levels in rats with Walker 256 carcinosarcoma[J].Anticancer Research,2012(32):129-133.
[14] Lv J,Sun X,Ma J,et al.Involvement of periostin-sclerostin-Wnt/beta-catenin signaling pathway in the prevention of neurectomy-induced bone loss by naringin[J].Biochemical and Biophysical Research Communications,2015(468):587-593.
[15] Lee EJ,Kim DI,Kim WJ,et al.Naringin inhibits matrix metalloproteinase-9 expression and AKT phosphorylation in tumor necrosis factor-alpha-induced vascular smooth muscle cells[J].Molecular Nutrition & Food Research,2009(53):1582-1591.
[16] Wong RW,Rabie AB.Effect of naringin on bone cells[J].Journal of Orthopaedic Research,2006(24):2045-2050.
[2] Andersen GB,Knudsen A,Hager H,et al.miRNA profiling identifies deregulated miRNAs associated with osteosarcoma development and time to metastasis in two large cohorts[J].Molecular Oncology,2018(12):114-131.
[3] He Y,Ma J,Wang A,et al.A support vector machine and a random forest classifier indicates a 15-miRNA set related to osteosarcoma recurrence[J].OncoTargets and Therapy,2018(11):253-269.
[4] Song YZ,Li JF.Circular RNA hsa_circ_0001564 regulates osteosarcoma proliferation and apoptosis by acting miRNA sponge[J].Biochemical and Biophysical Research Communications,2018(495):2369-2375.
[5] Zhu H,Gao J,Wang L,et al.In vitro study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor-kappaB signaling pathway[J].Experimental and Therapeutic Medicine,2018(15):2643-2648.
[6] Li H,Yang B,Huang J,et al.Naringin inhibits growth potential of human triple-negative breast cancer cells by targeting beta-catenin signaling pathway[J].Toxicology Letters,2013(220):219-228.
[7] Ramesh E,Alshatwi AA.Naringin induces death receptor and mitochondria-mediated apoptosis in human cervical cancer (SiHa) cells[J].Food and Chemical Toxicology,2013(51):97-105.
[8] Raha S,Yumnam S,Hong GE,et al.Naringin induces autophagy-mediated growth inhibition by downregulating the PI3K/Akt/mTOR cascade via activation of MAPK pathways in AGS cancer cells[J].International Journal of Oncology,2015(47):1061-1069.
[9] Li T,Xiao Y,Huang T.HIF1alpha induced upregulation of lncRNA UCA1 promotes cell growth in osteosarcoma by inactivating the PTEN/AKT signaling pathway[J].Oncology Reports,2018(39):1072-1080.
[10] Zhao Z,Jia Q,Wu MS,et al.Degalactotigonin,a natural compound from solanum nigrum L,inhibits growth and metastasis of osteosarcoma through GSK3beta inactivation-mediated repression of the Hedgehog/Gli1 pathway[J].Clinical Cancer Research,2018(24):130-144.
[11] Yoshinaga A,Kajiya N,Oishi K,et al.NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation[J].European Journal of Pharmacology,2016(782):21-29.
[12] Zeng L,Zhen Y,Chen Y,et al.Naringin inhibits growth and induces apoptosis by a mechanism dependent on reduced activation of NFkappaB/COX2caspase-1 pathway in HeLa cervical cancer cells[J].International Journal of Oncology,2014(45):1929-1936.
[13] Camargo CA,Gomes-Marcondes MC,Wutzki NC,et al.Naringin inhibits tumor growth and reduces interleukin-6 and tumor necrosis factor alpha levels in rats with Walker 256 carcinosarcoma[J].Anticancer Research,2012(32):129-133.
[14] Lv J,Sun X,Ma J,et al.Involvement of periostin-sclerostin-Wnt/beta-catenin signaling pathway in the prevention of neurectomy-induced bone loss by naringin[J].Biochemical and Biophysical Research Communications,2015(468):587-593.
[15] Lee EJ,Kim DI,Kim WJ,et al.Naringin inhibits matrix metalloproteinase-9 expression and AKT phosphorylation in tumor necrosis factor-alpha-induced vascular smooth muscle cells[J].Molecular Nutrition & Food Research,2009(53):1582-1591.
[16] Wong RW,Rabie AB.Effect of naringin on bone cells[J].Journal of Orthopaedic Research,2006(24):2045-2050.