MscL通道在生物纳米技术领域的应用研究
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摘要
大电导机械敏感性离子通道(Mechanosensitive channel of large conductance, MscL)是细菌上的一种机械敏感性离子通道,起到紧急释放阀门的作用,避免细菌在外界渗透压剧烈下降时破裂死亡。MscL开放口径大,易于修饰、突变和表达,重构于脂质体上仍有活性,是生物纳米技术领域良好的工具分子。近年来MscL在生物纳米技术领域的应用已有大量成果,研究发现通过修饰、突变后的MscL蛋白可以作为纳米给药系统上的分子开关,具有通透孔径和电荷的选择性,并受到光、pH及磁场等环境因素调控。对MscL在生物纳米技术领域的应用研究进行综述。
The mechanosensitive channel of large conductance(MscL) functions as an emergency valve in bacteria responding to changes in membrane tension and preventing lysis under acute osmotic shock. Taking the advantage of large pore size, tractable manipulation, and retainment of its mechanosensitivity when reconstituted into lipid bilayer, MscL could be developed into a ideal bio-nano tool. MscL based nanotechnology has advanced recently. These findings demonstrate that MscL channels with modified pore size or ion selectivity could be controlled by light, pH and magnetic field, and could be used for targeted drug release or other nanodevices. In this review, we focus on the progress of MscL based applictions in the field of bionanotechnology.
The mechanosensitive channel of large conductance(MscL) functions as an emergency valve in bacteria responding to changes in membrane tension and preventing lysis under acute osmotic shock. Taking the advantage of large pore size, tractable manipulation, and retainment of its mechanosensitivity when reconstituted into lipid bilayer, MscL could be developed into a ideal bio-nano tool. MscL based nanotechnology has advanced recently. These findings demonstrate that MscL channels with modified pore size or ion selectivity could be controlled by light, pH and magnetic field, and could be used for targeted drug release or other nanodevices. In this review, we focus on the progress of MscL based applictions in the field of bionanotechnology.
引文
[1]ESTANQUEIRO M,AMARAL M H,CONCEICAO J,et al. Nanotechnological carriers for cancer chemotherapy:the state of the art[J]. Colloids Surf B:Biointerfaces,2015,126:631-648.
[2]POON W,ZHANG X,NADEAU J. Nanoparticle drug formulations for cancer diagnosis and treatment[J]. Crit Rev Oncog,2014,19:223-245.
[3]SEETA RAMA RAJU G,BENTON L,PAVITRA E,et al. Multifunctional nanoparticles:recent progress in cancer therapeutics[J].Chem Commun(Camb),2015,51:13248-13259.
[4]LIONG M,LU J,KOVOCHICH M,et al. Multifunctional inorganic nanoparticles for imaging,targeting,and drug delivery[J]. ACS Nano,2008,2:889-896.
[5]HWANG A A,LU J,TAMANOI F,et al. Functional nanovalves on protein-coated nanoparticles for in vitro and in vivo controlled drug delivery[J]. Small,2015,11:319-328.
[6]FERRIS D P,LU J,GOTHARD C,et al. Synthesis of biomoleculemodified mesoporous silica nanoparticles for targeted hydrophobic drug delivery to cancer cells[J]. Small,2011,7:1816-1826.
[7]AMBROGIO M W,THOMAS C R,ZHAO Y L,et al. Mechanized silica nanoparticles:a new frontier in theranostic nanomedicine[J].Accounts of Chemical Research,2011,44:903-913.
[8]ZHANG W K,WANG D,DUAN Y Y,et al. Mechanosensitive gating of CFTR[J]. Nature Cell Biology,2010,12:507-512.
[9]HUANG D Y,YU B W. Recent advance and possible future in TREK-2:a two-pore potassium channel may involved in the process of NPP,brain ischemia and memory impairment[J]. Medical Hypotheses,2008,70:618-624.
[10]DEDMAN A,SHARIF-NAEINI R,FOLGERING J H,et al. The mechano-gated K2P channel TREK-1[J]. European Biophysics Journal,2009,38:293-303.
[11]INOUE R,JIAN Z,KAWARABAYASHI Y. Mechanosensitive TRP channels in cardiovascular pathophysiology[J]. Pharmacology&Therapeutic,2009,123:371-385.
[12]FOLGERING J H,SHARIF-NAEINI R,DEDMAN A,et al. Molecular basis of the mammalian pressure-sensitive ion channels:focus on vascular mechanotransduction[J]. Progess in Biophysics and Molocular Biology,2008,97:180-195.
[13]STIBER J A,SETH M,ROSENBERG P B. Mechanosensitive channels in striated muscle and the cardiovascular system:Not quite a stretch anymore[J]. Journal of Cardiovascular Pharmacology,2009,54:116-122.
[14]CORRY B,MARTINAC B. Bacterial mechanosensitive channels:experiment and theory[J]. Biochimica et Biophysica Acta(BBA)-Biomembranes,2008,1778(9):1859-1870.
[15]BOOTH I R,EDWARDS M D,BLACK S,et al. Mechanosensitive channels in bacteria:signs of closure?[J]. Nature Review Microbiology,2007,5:431-440.
[16]PEROZO E. Gating prokaryotic mechanosensitive channels[J].Nature Reviews Molecular Cell Biology,2006,7:108-119.
[17]YANG L M,WRAY R,PARKER J,et al. Three routes to modulate the pore size of the msc L channel/nanovalve[J]. ACS Nano,2012,6(2):1134-1141.
[18]PETROV G,ROHDE P R,MARTINAC B. Flying-patch patchclamp study of G22E-Msc L mutant under high hydrostatic pressure[J]. Biophysical Journal,2011,100:1635-1641.
[19]PARK K H,BERRIER C,MARTINAC B,et al. Purification and functional reconstitution of N-and C-halves of the Msc L channel[J]. Biophysical Journal,2004,86(4):2129-2136.
[20]SUKHAREV S,DURELL S R,GUY H R. Structural models of the Msc L gating mechanism[J]. Biophysical Journal,2001,81(2):917-36.
[21]ICHIMURA K,OH S K,NAKAGAWA M. Light-driven motion of liquids on a photoresponsive surface[J]. Science, 2000,288(5471):1624-1626.
[22]FOLGERING J H,KUIPER J M,DE VRIES A H,et al. Lipidmediated light activation of a mechanosensitive channel of large conductance[J]. Langmuir,2004,20(17):6985-6987.
[23]KO■ER A,WALKO M,MEIJBERG W,et al. A light-actuated nanovalve derived from a channel protein[J]. Science,2005,309:755-758.
[24]YOSHIMURA K,BATIZA A,KUNG C. Chemically charging the pore constriction opens the mechanosensitive channel Msc L[J].Biophysical Journal,2001,80(5):2198-206.
[25]BARTLETT J L,LEVIN G,BLOUNT P. An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating[J]. PNAS,2004,101(27):10161-10165.
[26]KO■ER A,WALKO M,BULTEN E,et al. Rationally designed chemical modulators convert a bacterial channel protein into a p Hsensory valve[J]. Angew Chem,2006,45:3126-3130.
[27]NAKAYAMA Y,MUSTAPI■M,EBRAHIMIAN H,et al. Magnetic nanoparticles for"smart liposomes"[J]. European Biophysics Journal,2015,44(8):647-654.
[28]LEVIN G,BLOUNT P. Cysteine scanning of Msc L transmembrane domains reveals residues critical for mechanosensitive channel gating[J]. Biophysical Journal,2004,86(5):2862-2870.
[29]LI Y,WRAY R,EATON C,BLOUNT P. An open-pore structure of the mechanosensitive channel Msc L derived by determining transmembrane domain interactions upon gating[J]. FASEB Journal,2009,23(7):2197-2204.
[30]YANG L M,BLOUNT P. Manipulating the permeation of charged compounds through the Msc L nanovalve[J]. FASEB Journal,2011,25(1):428-434.
[31]SUKHAREV S,BLOUNT P,MARTINAC B,et al. A large-conductance mechanosensitive channel in E. coli encoded by msc L alone[J]. Nature,1994,368(6468):265-268.
[32]ISCLA I,EATON C,PARKER J,et al. Improving the design of a msc L-based triggered nanovalve[J]. Biosensors(Basel),2013,3(1):171-184.
[33]LEE S F,ZHU X M,WANG Y X,et al. Ultrasound,p H,and magnetically responsive crown-ether-coated core/shell nanoparticles as drug encapsulation and release systems[J]. ACS Applied Materials&Interfaces,2013,5(5):1566-1574.
[34]BACHIN D,NAZARENKO L V,MIRONOV K S,et al. Mechanosensitive ion channel Msc L controls ionic fluxes during cold and heat stress in Synechocystis[J]. FEMS Microbiol Lett,2015,362(12):fnv090:1-6.
[35]HAL■A E,BRO T H,BILENBERG B,et al. Well-defined microapertures for ion channel biosensors[J]. Analytical Chemistry,2013,85(2):811-815.
[2]POON W,ZHANG X,NADEAU J. Nanoparticle drug formulations for cancer diagnosis and treatment[J]. Crit Rev Oncog,2014,19:223-245.
[3]SEETA RAMA RAJU G,BENTON L,PAVITRA E,et al. Multifunctional nanoparticles:recent progress in cancer therapeutics[J].Chem Commun(Camb),2015,51:13248-13259.
[4]LIONG M,LU J,KOVOCHICH M,et al. Multifunctional inorganic nanoparticles for imaging,targeting,and drug delivery[J]. ACS Nano,2008,2:889-896.
[5]HWANG A A,LU J,TAMANOI F,et al. Functional nanovalves on protein-coated nanoparticles for in vitro and in vivo controlled drug delivery[J]. Small,2015,11:319-328.
[6]FERRIS D P,LU J,GOTHARD C,et al. Synthesis of biomoleculemodified mesoporous silica nanoparticles for targeted hydrophobic drug delivery to cancer cells[J]. Small,2011,7:1816-1826.
[7]AMBROGIO M W,THOMAS C R,ZHAO Y L,et al. Mechanized silica nanoparticles:a new frontier in theranostic nanomedicine[J].Accounts of Chemical Research,2011,44:903-913.
[8]ZHANG W K,WANG D,DUAN Y Y,et al. Mechanosensitive gating of CFTR[J]. Nature Cell Biology,2010,12:507-512.
[9]HUANG D Y,YU B W. Recent advance and possible future in TREK-2:a two-pore potassium channel may involved in the process of NPP,brain ischemia and memory impairment[J]. Medical Hypotheses,2008,70:618-624.
[10]DEDMAN A,SHARIF-NAEINI R,FOLGERING J H,et al. The mechano-gated K2P channel TREK-1[J]. European Biophysics Journal,2009,38:293-303.
[11]INOUE R,JIAN Z,KAWARABAYASHI Y. Mechanosensitive TRP channels in cardiovascular pathophysiology[J]. Pharmacology&Therapeutic,2009,123:371-385.
[12]FOLGERING J H,SHARIF-NAEINI R,DEDMAN A,et al. Molecular basis of the mammalian pressure-sensitive ion channels:focus on vascular mechanotransduction[J]. Progess in Biophysics and Molocular Biology,2008,97:180-195.
[13]STIBER J A,SETH M,ROSENBERG P B. Mechanosensitive channels in striated muscle and the cardiovascular system:Not quite a stretch anymore[J]. Journal of Cardiovascular Pharmacology,2009,54:116-122.
[14]CORRY B,MARTINAC B. Bacterial mechanosensitive channels:experiment and theory[J]. Biochimica et Biophysica Acta(BBA)-Biomembranes,2008,1778(9):1859-1870.
[15]BOOTH I R,EDWARDS M D,BLACK S,et al. Mechanosensitive channels in bacteria:signs of closure?[J]. Nature Review Microbiology,2007,5:431-440.
[16]PEROZO E. Gating prokaryotic mechanosensitive channels[J].Nature Reviews Molecular Cell Biology,2006,7:108-119.
[17]YANG L M,WRAY R,PARKER J,et al. Three routes to modulate the pore size of the msc L channel/nanovalve[J]. ACS Nano,2012,6(2):1134-1141.
[18]PETROV G,ROHDE P R,MARTINAC B. Flying-patch patchclamp study of G22E-Msc L mutant under high hydrostatic pressure[J]. Biophysical Journal,2011,100:1635-1641.
[19]PARK K H,BERRIER C,MARTINAC B,et al. Purification and functional reconstitution of N-and C-halves of the Msc L channel[J]. Biophysical Journal,2004,86(4):2129-2136.
[20]SUKHAREV S,DURELL S R,GUY H R. Structural models of the Msc L gating mechanism[J]. Biophysical Journal,2001,81(2):917-36.
[21]ICHIMURA K,OH S K,NAKAGAWA M. Light-driven motion of liquids on a photoresponsive surface[J]. Science, 2000,288(5471):1624-1626.
[22]FOLGERING J H,KUIPER J M,DE VRIES A H,et al. Lipidmediated light activation of a mechanosensitive channel of large conductance[J]. Langmuir,2004,20(17):6985-6987.
[23]KO■ER A,WALKO M,MEIJBERG W,et al. A light-actuated nanovalve derived from a channel protein[J]. Science,2005,309:755-758.
[24]YOSHIMURA K,BATIZA A,KUNG C. Chemically charging the pore constriction opens the mechanosensitive channel Msc L[J].Biophysical Journal,2001,80(5):2198-206.
[25]BARTLETT J L,LEVIN G,BLOUNT P. An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating[J]. PNAS,2004,101(27):10161-10165.
[26]KO■ER A,WALKO M,BULTEN E,et al. Rationally designed chemical modulators convert a bacterial channel protein into a p Hsensory valve[J]. Angew Chem,2006,45:3126-3130.
[27]NAKAYAMA Y,MUSTAPI■M,EBRAHIMIAN H,et al. Magnetic nanoparticles for"smart liposomes"[J]. European Biophysics Journal,2015,44(8):647-654.
[28]LEVIN G,BLOUNT P. Cysteine scanning of Msc L transmembrane domains reveals residues critical for mechanosensitive channel gating[J]. Biophysical Journal,2004,86(5):2862-2870.
[29]LI Y,WRAY R,EATON C,BLOUNT P. An open-pore structure of the mechanosensitive channel Msc L derived by determining transmembrane domain interactions upon gating[J]. FASEB Journal,2009,23(7):2197-2204.
[30]YANG L M,BLOUNT P. Manipulating the permeation of charged compounds through the Msc L nanovalve[J]. FASEB Journal,2011,25(1):428-434.
[31]SUKHAREV S,BLOUNT P,MARTINAC B,et al. A large-conductance mechanosensitive channel in E. coli encoded by msc L alone[J]. Nature,1994,368(6468):265-268.
[32]ISCLA I,EATON C,PARKER J,et al. Improving the design of a msc L-based triggered nanovalve[J]. Biosensors(Basel),2013,3(1):171-184.
[33]LEE S F,ZHU X M,WANG Y X,et al. Ultrasound,p H,and magnetically responsive crown-ether-coated core/shell nanoparticles as drug encapsulation and release systems[J]. ACS Applied Materials&Interfaces,2013,5(5):1566-1574.
[34]BACHIN D,NAZARENKO L V,MIRONOV K S,et al. Mechanosensitive ion channel Msc L controls ionic fluxes during cold and heat stress in Synechocystis[J]. FEMS Microbiol Lett,2015,362(12):fnv090:1-6.
[35]HAL■A E,BRO T H,BILENBERG B,et al. Well-defined microapertures for ion channel biosensors[J]. Analytical Chemistry,2013,85(2):811-815.