碳酸钠碱性介质氧脱木素促进火炬松酶解糖化的研究
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摘要
分别采用硫酸盐法(KP)和绿液法(GL)蒸煮火炬松,并以碳酸钠为碱性介质进行氧脱木素,所得浆料分别标记为KP-OC浆和GL-OC浆,分析了这两种浆料的化学组分,并对这两种浆料进行酶解以提取可发酵单糖。结果显示,在120℃氧脱木素4 h所得的KP-OC浆和GL-OC浆的木素脱出率均明显优于相近条件下以氢氧化钠为碱性介质且脱木素1 h的浆料。对于GL-OC浆和KP-OC浆,当酶用量为10 FPU/g时,酶解单糖得率、总单糖提取率最高分别可达73.2%和95.8%、72.4%和75.7%,均优于相近条件下以氢氧化钠为碱性介质氧脱木素的浆料。
Loblolly pine were prepared by kraft process(KP) and green liquor process(GL),and the pulps were respectively carried out oxygen delignification with sodium carbonate as alkaline medium(marked as KP-OCand GL-OC),the chemical compositions of the resultant pulps were analyzed and the pulp were conducted enzymatic hydrolysis.The results indicated that the ratios of delignification of the pulps obtained through oxygen delignification at 120℃ for 4 h using sodium carbonate as alkaline medium were much higher than that of the pulp obtained through oxygen delignification at the similar conditions for 1 h using sodium hydroxide as alkaline medium.When the enzyme dosage was 10 FPU/g,the monoce yield and monoce recovery of enzymatic hydrolysis of GL-OC were 73.2% and 72.4%,respectively.Accordingly,those of KP-OC were 95.8% and 75.7%,respectively.The enzymatic hydrolysis efficiency of the pulp oxygen delignified with sodium carbonate was much better than that with sodium hydroxide.
Loblolly pine were prepared by kraft process(KP) and green liquor process(GL),and the pulps were respectively carried out oxygen delignification with sodium carbonate as alkaline medium(marked as KP-OCand GL-OC),the chemical compositions of the resultant pulps were analyzed and the pulp were conducted enzymatic hydrolysis.The results indicated that the ratios of delignification of the pulps obtained through oxygen delignification at 120℃ for 4 h using sodium carbonate as alkaline medium were much higher than that of the pulp obtained through oxygen delignification at the similar conditions for 1 h using sodium hydroxide as alkaline medium.When the enzyme dosage was 10 FPU/g,the monoce yield and monoce recovery of enzymatic hydrolysis of GL-OC were 73.2% and 72.4%,respectively.Accordingly,those of KP-OC were 95.8% and 75.7%,respectively.The enzymatic hydrolysis efficiency of the pulp oxygen delignified with sodium carbonate was much better than that with sodium hydroxide.
引文
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[15]Ko J K,Bak J S,Jung M,et al.Ethanol production from rice straw using optimized aqueous ammonia soaking pretreatment and simultaneous saccharification and fermentation[J].Bioresource Technology,2009,100(19):4374.
[16]Zhu J Y,Pan X J,Wang G S,et al.Sulfite pretreatment(SPORL)for robust enzymatic saccharification of spruce and red pine[J].Bioresource Technology,2009,100(8):2411.
[17]Salehian P,Karimi K,Zilouei H,et al.Improvement of biogas production from pine wood by alkali pretreatment[J].Fuel,2013,106(4):484.
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[19]Wu S,Jameel H,Chang H,et al.Techno-economic analysis of the optimum softwood lignin content for the production of bioethanol in a repurposed kraft mill[J].BioResources,2014,9(4):6817.
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