化妆品中耐热大肠菌群快速检测方法的研究
|
摘要
研究化妆品中耐热大肠菌群快速检测方法。分别采用3M Petrifilm~(TM)大肠菌群测试片法和环介导等温扩增(Loopmediated isothermal amplification,LAMP)技术检测人工污染化妆品中耐热大肠菌群,并与传统平板检测法进行了比较。结果表明,3M Petrifilm~(TM)大肠菌群测试片法与传统平板检测法比较结果无统计学差异,且操作步骤简便;LAMP技术特异性强,灵敏度可达到2.03 μg/L,且耗时短。3M Petrifilm~(TM)大肠菌群测试片法和LAMP技术均可用于化妆品中耐热大肠菌群的快速检测。
The study aimed to investigate the rapid detection method of thermotolerant coliforms in cosmetics. The 3M Petrifilm~(TM) coliform count plate method and Loop-mediated isothermal amplification(LAMP)technique were used to rapidly detect thermotolerant coliform bacteria in artificially contaminated cosmetics, and compared with the traditional flat plate detection method. The results show that the 3M Petrifilm~(TM) coliform test method and the traditional detection method have no statistical difference, and the operation procedure is simple. The LAMP technique has strong specificity, sensitivity of 2.03 μg/L, and is time-consuming. 3M Petrifilm TM coliform count plate method and LAMP technique can be used for rapid detection of thermotolerant coliforms in cosmetics.
The study aimed to investigate the rapid detection method of thermotolerant coliforms in cosmetics. The 3M Petrifilm~(TM) coliform count plate method and Loop-mediated isothermal amplification(LAMP)technique were used to rapidly detect thermotolerant coliform bacteria in artificially contaminated cosmetics, and compared with the traditional flat plate detection method. The results show that the 3M Petrifilm~(TM) coliform test method and the traditional detection method have no statistical difference, and the operation procedure is simple. The LAMP technique has strong specificity, sensitivity of 2.03 μg/L, and is time-consuming. 3M Petrifilm TM coliform count plate method and LAMP technique can be used for rapid detection of thermotolerant coliforms in cosmetics.
引文
[1]Li Zhiming.Food hygiene microbiological examination[M].Beijing:Chemical Industry Press,2009.
[2]State Food and Drug Administration.Safety and technical standards for cosmetics[S].Beijing:State Food and Drug Administration,2015.
[3]Sun Hui,Li Tiantian.Comparison of Petrifilm and GB methods in the food coliform bacteria detection[J].Liquor Making,2011,38(6):54-56.
[4]Bo Lichao,Li Fen,Zhang Jing.Research on the application of Petrifilm colony count test piece in disinfection quality monitoring of primary medical institutions[J].Chinese Journal of Disinfection,2018(3):180-185.
[5]Yang Lanhua,Wang Xuqiang,Lu Li,et al.Comparison of GBstandard with 3M Petrifilm method to test aerobic bacterial count in cosmetics[J].Chinese Journal of Health Laboratory Technology,2008(1):2297-2298.
[6]Zhu Shizhen,Wang Lifang,Wang Zunwen.Application of Petrifilm colony count plate in microbial detection for cosmetics[J].China Pharmacist,2014(9):1601-1602.
[7]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Research,2000,28(12):63.
[8]Kumar S,Mondal K K.Visual detection of Escherichia coli contamination in milk and fruit juice using loop-mediated isothermal amplification[J].Journal of Food Science and Technology,2015,52(11):7417-7424.
[9]Jiang Yong,Liu Zhentao,Ma Shumian,et al.Comparative study of different inoculation methods for detection of heat-injured coliforms in food[J].Journal of Food Safety and Quality,2018,9(14):3745-3748.
[10]Notomi T,Mori Y,Tomita N,et al.Loop-mediated isothermal amplific ation(LAMP):principle,features,and future prospects[J].Journal of Microbiology,2015,53(1):1-5.
[11]Yan M,Xu L,Jiang H,et al.PMA-LAMP for rapid detection of Escherichia coli and shiga toxins from viable but non-culturable state[J].Microbial Pathogenesis,2017,105:245-250.
[12]Liu W,Huang S,Liu N,et al.Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay[J].Scientific Reports,2017,7:40125.
[13]Yang Luxi,Li Xiaoyan.LAMP quantitative method and process based on a fluorescence quantitative system:201810265404.1[P].2018-08-10.
[2]State Food and Drug Administration.Safety and technical standards for cosmetics[S].Beijing:State Food and Drug Administration,2015.
[3]Sun Hui,Li Tiantian.Comparison of Petrifilm and GB methods in the food coliform bacteria detection[J].Liquor Making,2011,38(6):54-56.
[4]Bo Lichao,Li Fen,Zhang Jing.Research on the application of Petrifilm colony count test piece in disinfection quality monitoring of primary medical institutions[J].Chinese Journal of Disinfection,2018(3):180-185.
[5]Yang Lanhua,Wang Xuqiang,Lu Li,et al.Comparison of GBstandard with 3M Petrifilm method to test aerobic bacterial count in cosmetics[J].Chinese Journal of Health Laboratory Technology,2008(1):2297-2298.
[6]Zhu Shizhen,Wang Lifang,Wang Zunwen.Application of Petrifilm colony count plate in microbial detection for cosmetics[J].China Pharmacist,2014(9):1601-1602.
[7]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Research,2000,28(12):63.
[8]Kumar S,Mondal K K.Visual detection of Escherichia coli contamination in milk and fruit juice using loop-mediated isothermal amplification[J].Journal of Food Science and Technology,2015,52(11):7417-7424.
[9]Jiang Yong,Liu Zhentao,Ma Shumian,et al.Comparative study of different inoculation methods for detection of heat-injured coliforms in food[J].Journal of Food Safety and Quality,2018,9(14):3745-3748.
[10]Notomi T,Mori Y,Tomita N,et al.Loop-mediated isothermal amplific ation(LAMP):principle,features,and future prospects[J].Journal of Microbiology,2015,53(1):1-5.
[11]Yan M,Xu L,Jiang H,et al.PMA-LAMP for rapid detection of Escherichia coli and shiga toxins from viable but non-culturable state[J].Microbial Pathogenesis,2017,105:245-250.
[12]Liu W,Huang S,Liu N,et al.Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay[J].Scientific Reports,2017,7:40125.
[13]Yang Luxi,Li Xiaoyan.LAMP quantitative method and process based on a fluorescence quantitative system:201810265404.1[P].2018-08-10.