TET1和5hmC在上皮性卵巢癌组织中的表达及临床意义
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摘要
目的探讨TET1和5hm C在浆液上皮性卵巢癌组织中的表达及其与临床病理特点的关系。方法选取2016年02月至2017年02月间广西医科大学肿瘤医院收治的59例卵巢浆液上皮性组织样本,其中,浆液性囊腺癌组织30例,交界性浆液性囊腺瘤组织7例,浆液性囊腺瘤组织12例,并选取正常卵巢组织10例作为对照。采用免疫组化法观察TET1和5hm C蛋白的表达,采用Fisher和Spearman检验法进行相关性分析。结果与对照组相比,TET1和5hm C在囊腺癌组织中的表达升高最显著,差异均有统计学意义(均P<0.05)。在囊腺癌组织中,TET1在有淋巴结转移、病理学Ⅲ期和组织学高级别中的表达均高于无淋巴结转移、病理学Ⅰ~Ⅱ期和组织学低级别,差异均有统计学意义(均P<0.05)。结论 TET1和5hm C在浆液上皮性卵巢癌组织中高表达,TET1高表达与伴有淋巴结转移、病理学晚期和高组织学级别有关。
Objective To explore the expression levels of TET1 and 5 hm C in serous epithelial ovarian cancer tissues and the relationship with clinicopathological characteristics. Methods From February 2016 to February 2017,59 specimens of epithelial ovarian cancer were selected from patients treated at Guangxi Medical University with 30 specimens of serous cystadenocarcinoma,7 specimens of borderline serous cystadenoma,and 12 specimens of serous cystadenoma. The expression of TET1,5 hm C were observed by using immunohistochemical method. Fisher's exact test and spearman's test were used for statistical analysis. Results Compared with the control group,the expression levels of TET1 and 5 hm C were highest in serous ovarian cancer tissues( all P < 0. 05). High levels of TET1 were seen in lymphatic metastasis,pathology Ⅲ period and advanced histology groups in serous ovarian cancer tissues( all P < 0. 05).Conclusion The expression of TET1,5 hm C were high in serous epithelial ovarian cancer tissues. The high expression of TET1 was associated with lymphatic metastasis,pathology Ⅲ period and advanced histology.
Objective To explore the expression levels of TET1 and 5 hm C in serous epithelial ovarian cancer tissues and the relationship with clinicopathological characteristics. Methods From February 2016 to February 2017,59 specimens of epithelial ovarian cancer were selected from patients treated at Guangxi Medical University with 30 specimens of serous cystadenocarcinoma,7 specimens of borderline serous cystadenoma,and 12 specimens of serous cystadenoma. The expression of TET1,5 hm C were observed by using immunohistochemical method. Fisher's exact test and spearman's test were used for statistical analysis. Results Compared with the control group,the expression levels of TET1 and 5 hm C were highest in serous ovarian cancer tissues( all P < 0. 05). High levels of TET1 were seen in lymphatic metastasis,pathology Ⅲ period and advanced histology groups in serous ovarian cancer tissues( all P < 0. 05).Conclusion The expression of TET1,5 hm C were high in serous epithelial ovarian cancer tissues. The high expression of TET1 was associated with lymphatic metastasis,pathology Ⅲ period and advanced histology.
引文
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[14]Shi FT,Kim H,Lu W,et al.Ten-eleven translocation 1(Tet1)is regulated by O-linked N-acetylglucosamine transferase(Ogt)for target gene repression in mouse embryonic stem cells[J].J Biol Chem,2013,288:20776-20784.
[15]Chen Q,Yin D,Zhang Y,et al.MicroRNA-29a induces loss of 5-hydroxymethylcytosine and promotes metastasis of hepatocellular carcinoma through a TET-SOCS1-MMP9signaling axis[J].Cell Death Dis,2017,8:e2906.
[16]张萌萌,李雅琪,张硕,等.TET1蛋白对人乳腺癌MDA-MB-231细胞株增殖和侵袭能力的影响及其相关机制[J].肿瘤防治研究,2017,44:447-453.
[17]Good CR,Madzo J,Patel B,et al.Nucleic Acids Res.A novel isoform of TET1 that lacks a CXXC domain is overexpressed in cancer[J].Nucleic Acids Res,2017,45:8269-8281.
[2]Yokoyama S,Higashi M,Tsutsumida H,et al.TET1-mediated DNA hypomethylation regulates the expression of MUC4 in lung cancer[J].Genes Cancer,2017,8:517-527.
[3]Yang H,Liu Y,Bai F,et al.Tumor development is associated with decrease of TET gene expression and 5-methylcytosine hydroxylation[J].Oncogene,2013,32:663-669.
[4]沈凤,丁妍,谭玉洁.TET1基因敲除的宫颈癌Hela细胞增殖、侵袭能力观察[J].山东医药,2017,57:23-25.
[5]Bronowicka-Kys DE,Roszak A,Pawlik P,et al.Transcript levels of ten-eleven translocation type 1-3 in cervical cancer and non-cancerous cervical tissues[J].Oncol Lett,2017,13:3921-3927.
[6]Kok LF,Lee MY,Tyan YS,et al.Comparing the scoring mechanisms of p16INK4a immunohistochemistry based on independent nucleic stains and independent cytoplasmic stains in distinguishing between endocervical and endometrial adenocarcinomas in a tissue microarray study[J].Arch Gynecol Obstet,2010,281:293-300.
[7]Zhang H,Zhang X,Clark E,et al.TET1 is a DNA-binding protein that modulates DNA methylation and gene transcription via hydroxylation of 5-methylcytosine[J].Cell Res,2010,20:1390-1393.
[8]Huang H,Jiang X,Li Z,et al.TET1 plays an essential oncogenic role in MLL-rearranged leukemia[J].Proc Natl Acad Sci U S A,2013,110:11994-11999.
[9]Kudo Y,Tateishi K,Yamamoto K,et al.Loss of 5-hydroxymethylcytosine is accompanied with malignant cellular transformation[J].Cancer Sci,2012,103:670-676.
[10]Hsu CH,Peng KL,Kang ML,et al.TET1suppresses cancer invasion by activating the tissue inhibitors of metalloproteinases[J].Cell Rep,2012,2:568-579.
[11]Fu HL,Ma Y,Lu LG,et al.TET1 exerts its tumor suppressor function by interacting with p53-EZH2 pathway in gastric cancer[J].J Biomed Nanotechnol,2014,10:1217-1230.
[12]谢冰莹,张箴波,杨永彬,等.TET1在子宫内膜病变中的表达及其对癌细胞增殖和迁移能力的影响[J].上海交通大学学报(医学版),2015,35:316-322.
[13]Putiri EL,Tiedemann RL,Thompson JJ,et al.Distinct and over-lapping control of 5-methylcytosine and 5-hydroxymethylcytosine by the TET proteins in human cancer cells[J].Genome Biol,2014,15:R81.
[14]Shi FT,Kim H,Lu W,et al.Ten-eleven translocation 1(Tet1)is regulated by O-linked N-acetylglucosamine transferase(Ogt)for target gene repression in mouse embryonic stem cells[J].J Biol Chem,2013,288:20776-20784.
[15]Chen Q,Yin D,Zhang Y,et al.MicroRNA-29a induces loss of 5-hydroxymethylcytosine and promotes metastasis of hepatocellular carcinoma through a TET-SOCS1-MMP9signaling axis[J].Cell Death Dis,2017,8:e2906.
[16]张萌萌,李雅琪,张硕,等.TET1蛋白对人乳腺癌MDA-MB-231细胞株增殖和侵袭能力的影响及其相关机制[J].肿瘤防治研究,2017,44:447-453.
[17]Good CR,Madzo J,Patel B,et al.Nucleic Acids Res.A novel isoform of TET1 that lacks a CXXC domain is overexpressed in cancer[J].Nucleic Acids Res,2017,45:8269-8281.