真核生物来源漆酶的异源表达研究进展
|
摘要
漆酶属于多铜氧化酶家族中的一种,广泛存在于昆虫、植物、真菌和细菌中。由于其作用的底物范围较广,因此在纺织、制浆、食品以及木质素的降解等方面有广阔的应用前景。但是自然界中的漆酶存在表达量和酶活低、高温易失活等问题,限制了它的应用。对漆酶进行大量高效的异源表达,是解决这一问题的有效途径。近年来,越来越多不同来源的漆酶基因被克隆,并在不同宿主中异源表达。但这些大多局限于实验室研究,还未达到工业化生产的水平。笔者对真核生物来源漆酶的异源表达研究进展进行综述,重点介绍了真核生物来源的漆酶在不同表达系统中的异源表达情况以及在酵母细胞中表达漆酶时提高表达量和酶活性能的方法,以期为研究者们提供参考。
Laccases are enzymes belonging to the group of multi-copper oxidases.These enzymes are widely distributed in insects,plants,fungi and bacteria.In general,laccases can oxidize an exceptionally high number of substrates,so they have broad applications in textile,pulp,food and the degradation of lignin.However,low yield,low activity andthermo-instability of laccase in nature limit the application of laccase.High efficient heterologous expression of the protein is an effective way for solving this problem.Here,we summarize the research advances of heterologous expression of eukaryote-origin laccases.We focus on the overexpression of eukaryote-origin laccases using different expression system and the method for improving the production yield and enzyme activity in yeast cells.Information provided in this review would be helpful for researchers in the field.
Laccases are enzymes belonging to the group of multi-copper oxidases.These enzymes are widely distributed in insects,plants,fungi and bacteria.In general,laccases can oxidize an exceptionally high number of substrates,so they have broad applications in textile,pulp,food and the degradation of lignin.However,low yield,low activity andthermo-instability of laccase in nature limit the application of laccase.High efficient heterologous expression of the protein is an effective way for solving this problem.Here,we summarize the research advances of heterologous expression of eukaryote-origin laccases.We focus on the overexpression of eukaryote-origin laccases using different expression system and the method for improving the production yield and enzyme activity in yeast cells.Information provided in this review would be helpful for researchers in the field.
引文
[1]Thurston CF.The structure and function of fungal laccases.Microbiology,1994,140(1):19-26.
[2]Bao WL,O'Malley DM,Whetten R,et al.A laccase associated with lignification in loblolly pine xylem.Science,1993,260(5108):672-674.
[3]Driks A.Bacillus subtilis spore coat.Microbiol Mol Biol Rev,1999,63(1):1-20.
[4]Dittmer NT,Suderman RJ,Jiang HB,et al.Characterization of c DNAs encoding putative laccase-like multicopper oxidases and developmental expression in the tobacco hornworm,Manduca sexta,and the malaria mosquito,Anopheles gambiae.Insect Biochem Mol Biol,2004,34(1):29-41.
[5]Hullo MF,Moszer I,Danchin A,et al.Cot A of Bacillus subtilis is a copper-dependent laccase.JBacteriol,2001,183(18):5426-5430.
[6]Grass G,Rensing C.Cue O is a multi-copper oxidase that confers copper tolerance in Escherichia coli.Biochem Biophys Res Commun,2001,286(5):902-908.
[7]Claus H.Laccases and their occurrence in prokaryotes.Arch Microbiol,2003,179(3):145-150.
[8]Si J,Li W,Cui BK,et al.Advances of research on characteristic,molecular biology and applications of laccase from fungi.Biotechnol Bull,2011,(2):48-55(in Chinese).司静,李伟,崔宝凯,等.真菌漆酶性质、分子生物学及其应用研究迚展.生物技术通报,2011,(2):48-55.
[9]Ten Have R,Teunissen PJM.Oxidative mechanisms involved in lignin degradation by white-rot fungi.Chem Rev,2001,101(11):3397-3413.
[10]Baldrian P.Fungal laccases-occurrence and properties.FEMS Microbiol Rev,2006,30(2):215-242.
[11]Wang GD,Li QJ,Luo B,et al.Ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase.Nat Biotechnol,2004,22(7):893-897.
[12]Mayer AM,Staples RC.Laccase:new functions for an old enzyme.Phytochemistry,2002,60(6):551-565.
[13]Yu ML,Ni JF.Research advances in the insect laccase.Chin J Bioproc Eng,2014,12(1):80-85(in Chinese).于孟兰,倪金凤.昆虫漆酶的研究迚展.生物加工过程,2014,12(1):80-85.
[14]Giardina P,Faraco V,Pezzella C,et al.Laccases:a never-ending story.Cell Mol Life Sci,2010,67(3):369-385.
[15]Liu ZC,Wang GG.Fungal laccases:structure-based function and mechanism.Acta Biophys Sinica,2013,29(9):629-645(in Chinese).刘忠川,王刚刚.真菌漆酶结构与功能研究迚展.生物物理学报,2013,29(9):629-645.
[16]Anto?ováZ,SychrováH.Yeast hosts for the production of recombinant laccases:a review.Mol Biotechnol,2016,58(2):93-116.
[17]Piscitelli A,Pezzella C,Giardina P,et al.Heterologous laccase production and its role in industrial applications.Bioeng Bugs,2010,1(4):254-264.
[18]Ausec L,?rnigoj M,?najder M,et al.Characterization of a novel high-p H-tolerant laccase-like multicopper oxidase and its sequence diversity in Thioalkalivibrio sp..Appl Microbiol Biotechnol,2015,99(23):9987-9999.
[19]Zhou W,Guan ZB,Cai YJ,et al.Progress in the Bacillus laccase.Microbiol China,2015,42(7):1372-1383(in Chinese).周稳,管政兵,蔡宇杰,等.芽胞杆菌漆酶的研究迚展.微生物学通报,2015,42(7):1372-1383.
[20]Ma YY,Jia HH,Wei P.Progress in study and application of bacterial laccase.Biotechnol Bull,2013,(2):41-48(in Chinese).马莹莹,贾红华,韦萍.细菌漆酶的研究及应用迚展.生物技术通报,2013,(2):41-48.
[21]Yang JQ.Heterologous expression and modification of laccase from Panus rudis[D].Beijing:Academy of Military Medical Science,2005(in Chinese).杨建强.革耳(Panus rudis)漆酶的异源表达和酶学性质改良[D].北京:中国人民解放军军事医学科学院,2005.
[22]Salony,Garg N,Baranwal R,et al.Laccase of Cyathus bulleri:structural,catalytic characterization and expression in Escherichia coli.Biochim Biophys Acta Proteins Proteomics,2008,1784(2):259-268.
[23]Nicolini C,Bruzzese D,Cambria MT,et al.Recombinant laccase:I.Enzyme cloning and characterization.J Cell Biochem,2013,114(3):599-605.
[24]Pan CY.Cloning and expression of genes encoding laccases in Termitomyces albuminosus,Culex pipiens pallens and Musca domestica[D].Hangzhou:Zhejiang University,2009(in Chinese).潘程进.鸡枞菌与淡色库蚊及家蝇中漆酶编码基因的克隆与表达[D].杭州:浙江大学,2009.
[25]Xue D.Cloning laccase gene from edible fungl and established the system of transformation lactic acid bacteria by electroporation[D].Hohhot:Inner Mongolia University,2014(in Chinese).薛丹.食用菌中漆酶基因的克隆及乳酸菌电激转化体系的建立[D].呼和浩特:内蒙古大学,2014.
[26]Rodgers CJ,Blanford CF,Giddens SR,et al.Designer laccases:a vogue for high-potential fungal enzymes?Trends Biotechnol,2010,28(2):63-72.
[27]Maestre-Reyna M,Liu WC,Jeng WY,et al.Structural and functional roles of glycosylation in fungal laccase from Lentinus sp..PLo S ONE,2015,10(4):e0120601.
[28]Jadhav JP,Parshetti GK,Kalme SD,et al.Decolourization of azo dye methyl red by Saccharomyces cerevisiae MTCC 463.Chemosphere,2007,68(2):394-400.
[29]Kalyani D,Tiwari MK,Li JL,et al.A highly efficient recombinant laccase from the yeast Yarrowia lipolytica and its application in the hydrolysis of biomass.PLo S ONE,2015,10(3):e0120156.
[30]Pezzella C,Autore F,Giardina P,et al.The Pleurotus ostreatus laccase multi-gene family:isolation and heterologous expression of new family members.Curr Genet,2009,55(1):45-57.
[31]Gu CJ,Zheng F,Long LK,et al.Engineering the expression and characterization of two novel laccase isoenzymes from Coprinus comatus in Pichia pastoris by fusing an additional ten amino acids tag at N-terminus.PLo S ONE,2014,9(4):e93912.
[32]Bao WH,Peng RH,Zhang Z,et al.Expression,characterization and 2,4,6-trichlorophenol degradation of laccase from Monilinia fructigena.Mol Biol Rep,2012,39(4):3871-3877.
[33]Wang HW,Zhu H,Liang XF,et al.Molecular cloning and expression of a novel laccase showing thermo-and acid-stability from the endophytic fungus Phomopsis liquidambari and its potential for growth promotion of plants.Biotechnol Lett,2014,36(1):167-173.
[34]Wang B,Yan Y,Tian YS,et al.Heterologous expression and characterisation of a laccase from Colletotrichum lagenarium and decolourisation of different synthetic dyes.World J Microbiol Biotechnol,2016,32(3):40.
[35]Liu HP,Tong CF,Du B,et al.Expression and characterization of Lac MP,a novel fungal laccase of Moniliophthora perniciosa FA553.Biotechnol Lett,2015,37(9):1829-1835.
[36]Lu YP,Wu GM,Lian LD,et al.Cloning and expression analysis of Vvlcc3,a novel and functional laccase gene possibly involved in stipe elongation.Int J Mol Sci,2015,16(12):28498-28509.
[37]Yang J,Ng TB,Lin J,et al.A novel laccase from basidiomycete Cerrena sp.:cloning,heterologous expression,and characterization.Int J Biol Macromol,2015,77:344-349.
[38]Feng BZ,Li PQ.Cloning,characterization and expression of a novel laccase gene Pclac2 from Phytophthora capsici.Braz J Microbiol,2014,45(1):351-358.
[39]Tian YS,Xu H,Peng RH,et al.Heterologous expression and characterization of laccase 2 from Coprinopsis cinerea capable of decolourizing different recalcitrant dyes.Biotechnol Biotechnol Equip,2014,28(2):248-258.
[40]Wu L,Yang JH,Chen MJ,et al.Cloning and heterologous expression of laccase genes vv-lac1and vv-lac6 from Volvaria volvacea.Acta Microbiol Sin,2014,54(7):828-835(in Chinese).吴林,阳经慧,陈明杰,等.草菇漆酶基因vv-lac1和vv-lac6的克隆及异源表达.微生物学报,2014,54(7):828-835.
[41]You LF,Liu ZM,Lin JF,et al.Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris.J Basic Microbiol,2014,54 Suppl 1:S134-S141.
[42]Bao SY,Teng Z,Ding SJ.Heterologous expression and characterization of a novel laccase isoenzyme with dyes decolorization potential from Coprinus comatus.Mol Biol Rep,2013,40(2):1927-1936.
[43]Garg N,Bieler N,Kenzom T,et al.Cloning,sequence analysis,expression of Cyathusbulleri laccase in Pichia pastoris and characterization of recombinant laccase.BMC Biotechnol,2012,12:75.
[44]Kittl R,Gonaus C,Pillei C,et al.Constitutive expression of Botrytis aclada laccase in Pichia pastoris.Bioengineered,2012,3(4):232-235.
[45]Sun J,Peng RH,Xiong AS,et al.Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris.Mol Biol Rep,2012,39(4):3807-3814.
[46]Huang SJ,Liu ZM,Huang XL,et al.Molecular cloning and characterization of a novel laccase gene from a white-rot fungus Polyporus grammocephalus TR16 and expression in Pichia pastoris.Lett Appl Microbiol,2011,52(3):290-297.
[47]Bleve G,Lezzi C,Mita G,et al.Molecular cloning and heterologous expression of a laccase gene from Pleurotus eryngii in free and immobilized Saccharomyces cerevisiae cells.Appl Microbiol Biotechnol,2008,79(5):731-741.
[48]Faraco V,Ercole C,Festa G,et al.Heterologous expression of heterodimeric laccase from Pleurotus ostreatus in Kluyveromyces lactis.Appl Microbiol Biotechnol,2008,77(6):1329-1335.
[49]Joo SS,Ryu IW,Park JK,et al.Molecular cloning and expression of a laccase from Ganoderma lucidum,and its antioxidative properties.Mol Cells,2008,25(1):112-118.
[50]Hong YZ,Zhou HM,Tu XM,et al.Cloning of a laccase gene from a novel basidiomycete Trametes sp.420 and its heterologous expression in Pichia pastoris.Curr Microbiol,2007,54(4):260-265.
[51]Li JF,Hong YZ,Xiao YZ.Cloning and heterologous expression of the gene of laccase Cfrom Trametes sp.420 and potential of recombinant laccase in dye decolorization.Acta Microbiol Sin,2007,47(1):54-58(in Chinese).李剑凤,洪宇植,肖亚中.栓菌420漆酶C基因的克隆、高效表达及重组酶的染料脱色潜能.微生物学报,2007,47(1):54-58.
[52]Yaver DS,Xu F,Golightly EJ,et al.Purification,characterization,molecular cloning,and expression of two laccase genes from the white rot basidiomycete Trametes villosa.Appl Environ Microbiol,1996,62(3):834-841.
[53]Larrondo LF,Avila M,Salas L,et al.Heterologous expression of laccase c DNA from Ceriporiopsis subvermispora yields copper-activated apoprotein and complex isoform patterns.Microbiology,2003,149(5):1177-1182.
[54]Benghazi L,Record E,Suárez A,et al.Production of the Phanerochaete flavido-alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation.World J Microbiol Biotechnol,2014,30(1):201-211.
[55]Ramos JAT,Barends S,Verhaert RMD,et al.The Aspergillus niger multicopper oxidase family:analysis and overexpression of laccase-like encoding genes.Microb Cell Fact,2011,10:78.
[56]Tamayo-Ramos JA,Barends S,de Lange D,et al.Enhanced production of Aspergillus niger laccase-like multicopper oxidases through m RNAoptimization of the glucoamylase expression system.Biotechnol Bioeng,2013,110(2):543-551.
[57]Wang YC,Xue W,Sims AH,et al.Isolation of four pepsin-like protease genes from Aspergillus niger and analysis of the effect of disruptions on heterologous laccase expression.Fungal Genet Biol,2008,45(1):17-27.
[58]Kiiskinen LL,Kruus K,Bailey M,et al.Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme.Microbiology,2004,150(9):3065-3074.
[59]Zhang JW,Qu YB,Xiao P,et al.Improved biomass saccharification by Trichoderma reesei through heterologous expression of lac A gene from Trametes sp.AH28-2.J Biosci Bioeng,2012,113(6):697-703.
[60]Dong XR,Qin LN,Tao Y,et al.Overexpression and characterization of a laccase gene from Pleurotus ostreatus in Trichoderma reesei.Acta Microbiol Sin,2012,52(7):850-856(in Chinese).董欣睿,秦丼娜,陶勇,等.糙皮侧耳(Pleurotus ostreatus)漆酶POXA1在里氏木霉中的高效表达及酶学性质.微生物学报,2012,52(7):850-856.
[61]Abianova AR,Chulkin AM,Vavilova EA,et al.Aheterologous production of the Trametes hirsuta laccase in the fungus Penicillium canescens.Prikl Biokhim Mikrobiol,2010,46(3):342-347.
[62]Fujihiro S,Higuchi R,Hisamatsu S,et al.Metabolism of hydroxylated PCB congeners by cloned laccase isoforms.Appl Microbiol Biotechnol,2009,82(5):853-860.
[63]SkálováT,Dohnálek J,?stergaard LH,et al.The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases.J Mol Biol,2009,385(4):1165-1178.
[64]Andberg M,Hakulinen N,Auer S,et al.Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production,catalytic properties and structure.FEBS J,2009,276(21):6285-6300.
[65]Berka RM,Schneider P,Golightly EJ,et al.Characterization of the gene encoding an extracellular laccase of Myceliophthora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae.Appl Environ Microbiol,1997,63(8):3151-3157.
[66]Bukh C,Lund M,Bjerrum MJ.Kinetic studies on the reaction between Trametes villosa laccase and dioxygen.J Inorg Biochem,2006,100(9):1547-1557.
[67]Hakulinen N,Kruus K,Koivula A,et al.Acrystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase.Biochem Biophys Res Commun,2006,350(4):929-934.
[68]Sigoillot C,Camarero S,Vidal T,et al.Comparison of different fungal enzymes for bleaching high-quality paper pulps.J Biotechnol,2005,115(4):333-343.
[69]Ravalason H,Herpo?l-Gimbert I,Record E,et al.Fusion of a family 1 carbohydrate binding module of Aspergillus niger to the Pycnoporus cinnabarinus laccase for efficient softwood kraft pulp biobleaching.J Biotechnol,2009,142(3/4):220-226.
[70]Li L,Steffens JC.Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance.Planta,2002,215(2):239-247.
[71]Chiaiese P,Palomba F,Tatino F,et al.Engineered tobacco and microalgae secreting the fungal laccase POXA1b reduce phenol content in olive oil mill wastewater.Enzyme Microb Technol,2011,49(6/7):540-546.
[72]Cesarino I,Araújo P,Mayer JLS,et al.Expression of Sof LAC,a new laccase in sugarcane,restores lignin content but not S?G ratio of Arabidopsis lac17 mutant.J Exp Bot,2013,64(6):1769-1781.
[73]Arakane Y,Muthukrishnan S,Beeman RW,et al.Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning.Proc Natl Acad Sci USA,2005,102(32):11337-11342.
[74]Dittmer NT,Gorman MJ,Kanost MR.Characterization of endogenous and recombinant forms of laccase-2,a multicopper oxidase from the tobacco hornworm,Manduca sexta.Insect Biochem Mol Biol,2009,39(9):596-606.
[75]Coy MR,Salem TZ,Denton JS,et al.Phenol-oxidizing laccases from the termite gut.Insect Biochem Mol Biol,2010,40(10):723-732.
[76]Hong F,Meinander NQ,J?nsson LJ.Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris.Biotechnol Bioeng,2002,79(4):438-449.
[77]Guo M,Lu FP,Du LX,et al.Optimization of the expression of a laccase gene from Trametes versicolor in Pichia methanolica.Appl Microbiol Biotechnol,2006,71(6):848-852.
[78]Liu W,Chao Y,Liu S,et al.Molecular cloning and characterization of a laccase gene from the basidiomycete Fome lignosus and expression in Pichia pastoris.Appl Microbiol Biotechnol,2003,63(2):174-181.
[79]Rivera-Hoyos CM,Morales-álvarez ED,Poveda-Cuevas SA,et al.Computational analysis and low-scale constitutive expression of laccases synthetic genes Gl LCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris.PLo S ONE,2015,10(1):e0116524.
[80]Mate DM,Gonzalez-Perez D,Kittl R,et al.Functional expression of a blood tolerant laccase in Pichia pastoris.BMC Biotechnol,2013,13:38.
[81]Bohlin C,J?nsson LJ,Roth R,et al.Heterologous expression of Trametes versicolor laccase in Pichia pastoris and Aspergillus niger.Appl Biochem Biotechnol,2006,129(1/2/3):195-214.
[82]Brown MA,Zhao ZW,Mauk AG.Expression and characterization of a recombinant multi-copper oxidase:laccase IV from Trametes versicolor.Inorganica Chim Acta,2002,331(1):232-238.
[83]Guo M,Lu FP,Pu J,et al.Molecular cloning of the c DNA encoding laccase from Trametes versicolor and heterologous expression in Pichia methanolica.Appl Microbiol Biotechnol,2005,69(2):178-183.
[84]Hildén K,M?kel?MR,Lundell T,et al.Heterologous expression and structural characterization of two low p H laccases from a biopulping white-rot fungus Physisporinus rivulosus.Appl Microbiol Biotechnol,2013,97(4):1589-1599.
[85]Lin YQ,Zhang Z,Tian YS,et al.Purification and characterization of a novel laccase from Coprinus cinereus and decolorization of different chemically dyes.Mol Biol Rep,2013,40(2):1487-1494.
[86]Bulter T,Alcalde M,Sieber V,et al.Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution.Appl Environ Microbiol,2003,69(2):987-995.
[87]Festa G,Autore F,Fraternali F,et al.Development of new laccases by directed evolution:functional and computational analyses.Proteins Struct Funct Bioinform,2008,72(1):25-34.
[2]Bao WL,O'Malley DM,Whetten R,et al.A laccase associated with lignification in loblolly pine xylem.Science,1993,260(5108):672-674.
[3]Driks A.Bacillus subtilis spore coat.Microbiol Mol Biol Rev,1999,63(1):1-20.
[4]Dittmer NT,Suderman RJ,Jiang HB,et al.Characterization of c DNAs encoding putative laccase-like multicopper oxidases and developmental expression in the tobacco hornworm,Manduca sexta,and the malaria mosquito,Anopheles gambiae.Insect Biochem Mol Biol,2004,34(1):29-41.
[5]Hullo MF,Moszer I,Danchin A,et al.Cot A of Bacillus subtilis is a copper-dependent laccase.JBacteriol,2001,183(18):5426-5430.
[6]Grass G,Rensing C.Cue O is a multi-copper oxidase that confers copper tolerance in Escherichia coli.Biochem Biophys Res Commun,2001,286(5):902-908.
[7]Claus H.Laccases and their occurrence in prokaryotes.Arch Microbiol,2003,179(3):145-150.
[8]Si J,Li W,Cui BK,et al.Advances of research on characteristic,molecular biology and applications of laccase from fungi.Biotechnol Bull,2011,(2):48-55(in Chinese).司静,李伟,崔宝凯,等.真菌漆酶性质、分子生物学及其应用研究迚展.生物技术通报,2011,(2):48-55.
[9]Ten Have R,Teunissen PJM.Oxidative mechanisms involved in lignin degradation by white-rot fungi.Chem Rev,2001,101(11):3397-3413.
[10]Baldrian P.Fungal laccases-occurrence and properties.FEMS Microbiol Rev,2006,30(2):215-242.
[11]Wang GD,Li QJ,Luo B,et al.Ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase.Nat Biotechnol,2004,22(7):893-897.
[12]Mayer AM,Staples RC.Laccase:new functions for an old enzyme.Phytochemistry,2002,60(6):551-565.
[13]Yu ML,Ni JF.Research advances in the insect laccase.Chin J Bioproc Eng,2014,12(1):80-85(in Chinese).于孟兰,倪金凤.昆虫漆酶的研究迚展.生物加工过程,2014,12(1):80-85.
[14]Giardina P,Faraco V,Pezzella C,et al.Laccases:a never-ending story.Cell Mol Life Sci,2010,67(3):369-385.
[15]Liu ZC,Wang GG.Fungal laccases:structure-based function and mechanism.Acta Biophys Sinica,2013,29(9):629-645(in Chinese).刘忠川,王刚刚.真菌漆酶结构与功能研究迚展.生物物理学报,2013,29(9):629-645.
[16]Anto?ováZ,SychrováH.Yeast hosts for the production of recombinant laccases:a review.Mol Biotechnol,2016,58(2):93-116.
[17]Piscitelli A,Pezzella C,Giardina P,et al.Heterologous laccase production and its role in industrial applications.Bioeng Bugs,2010,1(4):254-264.
[18]Ausec L,?rnigoj M,?najder M,et al.Characterization of a novel high-p H-tolerant laccase-like multicopper oxidase and its sequence diversity in Thioalkalivibrio sp..Appl Microbiol Biotechnol,2015,99(23):9987-9999.
[19]Zhou W,Guan ZB,Cai YJ,et al.Progress in the Bacillus laccase.Microbiol China,2015,42(7):1372-1383(in Chinese).周稳,管政兵,蔡宇杰,等.芽胞杆菌漆酶的研究迚展.微生物学通报,2015,42(7):1372-1383.
[20]Ma YY,Jia HH,Wei P.Progress in study and application of bacterial laccase.Biotechnol Bull,2013,(2):41-48(in Chinese).马莹莹,贾红华,韦萍.细菌漆酶的研究及应用迚展.生物技术通报,2013,(2):41-48.
[21]Yang JQ.Heterologous expression and modification of laccase from Panus rudis[D].Beijing:Academy of Military Medical Science,2005(in Chinese).杨建强.革耳(Panus rudis)漆酶的异源表达和酶学性质改良[D].北京:中国人民解放军军事医学科学院,2005.
[22]Salony,Garg N,Baranwal R,et al.Laccase of Cyathus bulleri:structural,catalytic characterization and expression in Escherichia coli.Biochim Biophys Acta Proteins Proteomics,2008,1784(2):259-268.
[23]Nicolini C,Bruzzese D,Cambria MT,et al.Recombinant laccase:I.Enzyme cloning and characterization.J Cell Biochem,2013,114(3):599-605.
[24]Pan CY.Cloning and expression of genes encoding laccases in Termitomyces albuminosus,Culex pipiens pallens and Musca domestica[D].Hangzhou:Zhejiang University,2009(in Chinese).潘程进.鸡枞菌与淡色库蚊及家蝇中漆酶编码基因的克隆与表达[D].杭州:浙江大学,2009.
[25]Xue D.Cloning laccase gene from edible fungl and established the system of transformation lactic acid bacteria by electroporation[D].Hohhot:Inner Mongolia University,2014(in Chinese).薛丹.食用菌中漆酶基因的克隆及乳酸菌电激转化体系的建立[D].呼和浩特:内蒙古大学,2014.
[26]Rodgers CJ,Blanford CF,Giddens SR,et al.Designer laccases:a vogue for high-potential fungal enzymes?Trends Biotechnol,2010,28(2):63-72.
[27]Maestre-Reyna M,Liu WC,Jeng WY,et al.Structural and functional roles of glycosylation in fungal laccase from Lentinus sp..PLo S ONE,2015,10(4):e0120601.
[28]Jadhav JP,Parshetti GK,Kalme SD,et al.Decolourization of azo dye methyl red by Saccharomyces cerevisiae MTCC 463.Chemosphere,2007,68(2):394-400.
[29]Kalyani D,Tiwari MK,Li JL,et al.A highly efficient recombinant laccase from the yeast Yarrowia lipolytica and its application in the hydrolysis of biomass.PLo S ONE,2015,10(3):e0120156.
[30]Pezzella C,Autore F,Giardina P,et al.The Pleurotus ostreatus laccase multi-gene family:isolation and heterologous expression of new family members.Curr Genet,2009,55(1):45-57.
[31]Gu CJ,Zheng F,Long LK,et al.Engineering the expression and characterization of two novel laccase isoenzymes from Coprinus comatus in Pichia pastoris by fusing an additional ten amino acids tag at N-terminus.PLo S ONE,2014,9(4):e93912.
[32]Bao WH,Peng RH,Zhang Z,et al.Expression,characterization and 2,4,6-trichlorophenol degradation of laccase from Monilinia fructigena.Mol Biol Rep,2012,39(4):3871-3877.
[33]Wang HW,Zhu H,Liang XF,et al.Molecular cloning and expression of a novel laccase showing thermo-and acid-stability from the endophytic fungus Phomopsis liquidambari and its potential for growth promotion of plants.Biotechnol Lett,2014,36(1):167-173.
[34]Wang B,Yan Y,Tian YS,et al.Heterologous expression and characterisation of a laccase from Colletotrichum lagenarium and decolourisation of different synthetic dyes.World J Microbiol Biotechnol,2016,32(3):40.
[35]Liu HP,Tong CF,Du B,et al.Expression and characterization of Lac MP,a novel fungal laccase of Moniliophthora perniciosa FA553.Biotechnol Lett,2015,37(9):1829-1835.
[36]Lu YP,Wu GM,Lian LD,et al.Cloning and expression analysis of Vvlcc3,a novel and functional laccase gene possibly involved in stipe elongation.Int J Mol Sci,2015,16(12):28498-28509.
[37]Yang J,Ng TB,Lin J,et al.A novel laccase from basidiomycete Cerrena sp.:cloning,heterologous expression,and characterization.Int J Biol Macromol,2015,77:344-349.
[38]Feng BZ,Li PQ.Cloning,characterization and expression of a novel laccase gene Pclac2 from Phytophthora capsici.Braz J Microbiol,2014,45(1):351-358.
[39]Tian YS,Xu H,Peng RH,et al.Heterologous expression and characterization of laccase 2 from Coprinopsis cinerea capable of decolourizing different recalcitrant dyes.Biotechnol Biotechnol Equip,2014,28(2):248-258.
[40]Wu L,Yang JH,Chen MJ,et al.Cloning and heterologous expression of laccase genes vv-lac1and vv-lac6 from Volvaria volvacea.Acta Microbiol Sin,2014,54(7):828-835(in Chinese).吴林,阳经慧,陈明杰,等.草菇漆酶基因vv-lac1和vv-lac6的克隆及异源表达.微生物学报,2014,54(7):828-835.
[41]You LF,Liu ZM,Lin JF,et al.Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris.J Basic Microbiol,2014,54 Suppl 1:S134-S141.
[42]Bao SY,Teng Z,Ding SJ.Heterologous expression and characterization of a novel laccase isoenzyme with dyes decolorization potential from Coprinus comatus.Mol Biol Rep,2013,40(2):1927-1936.
[43]Garg N,Bieler N,Kenzom T,et al.Cloning,sequence analysis,expression of Cyathusbulleri laccase in Pichia pastoris and characterization of recombinant laccase.BMC Biotechnol,2012,12:75.
[44]Kittl R,Gonaus C,Pillei C,et al.Constitutive expression of Botrytis aclada laccase in Pichia pastoris.Bioengineered,2012,3(4):232-235.
[45]Sun J,Peng RH,Xiong AS,et al.Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris.Mol Biol Rep,2012,39(4):3807-3814.
[46]Huang SJ,Liu ZM,Huang XL,et al.Molecular cloning and characterization of a novel laccase gene from a white-rot fungus Polyporus grammocephalus TR16 and expression in Pichia pastoris.Lett Appl Microbiol,2011,52(3):290-297.
[47]Bleve G,Lezzi C,Mita G,et al.Molecular cloning and heterologous expression of a laccase gene from Pleurotus eryngii in free and immobilized Saccharomyces cerevisiae cells.Appl Microbiol Biotechnol,2008,79(5):731-741.
[48]Faraco V,Ercole C,Festa G,et al.Heterologous expression of heterodimeric laccase from Pleurotus ostreatus in Kluyveromyces lactis.Appl Microbiol Biotechnol,2008,77(6):1329-1335.
[49]Joo SS,Ryu IW,Park JK,et al.Molecular cloning and expression of a laccase from Ganoderma lucidum,and its antioxidative properties.Mol Cells,2008,25(1):112-118.
[50]Hong YZ,Zhou HM,Tu XM,et al.Cloning of a laccase gene from a novel basidiomycete Trametes sp.420 and its heterologous expression in Pichia pastoris.Curr Microbiol,2007,54(4):260-265.
[51]Li JF,Hong YZ,Xiao YZ.Cloning and heterologous expression of the gene of laccase Cfrom Trametes sp.420 and potential of recombinant laccase in dye decolorization.Acta Microbiol Sin,2007,47(1):54-58(in Chinese).李剑凤,洪宇植,肖亚中.栓菌420漆酶C基因的克隆、高效表达及重组酶的染料脱色潜能.微生物学报,2007,47(1):54-58.
[52]Yaver DS,Xu F,Golightly EJ,et al.Purification,characterization,molecular cloning,and expression of two laccase genes from the white rot basidiomycete Trametes villosa.Appl Environ Microbiol,1996,62(3):834-841.
[53]Larrondo LF,Avila M,Salas L,et al.Heterologous expression of laccase c DNA from Ceriporiopsis subvermispora yields copper-activated apoprotein and complex isoform patterns.Microbiology,2003,149(5):1177-1182.
[54]Benghazi L,Record E,Suárez A,et al.Production of the Phanerochaete flavido-alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation.World J Microbiol Biotechnol,2014,30(1):201-211.
[55]Ramos JAT,Barends S,Verhaert RMD,et al.The Aspergillus niger multicopper oxidase family:analysis and overexpression of laccase-like encoding genes.Microb Cell Fact,2011,10:78.
[56]Tamayo-Ramos JA,Barends S,de Lange D,et al.Enhanced production of Aspergillus niger laccase-like multicopper oxidases through m RNAoptimization of the glucoamylase expression system.Biotechnol Bioeng,2013,110(2):543-551.
[57]Wang YC,Xue W,Sims AH,et al.Isolation of four pepsin-like protease genes from Aspergillus niger and analysis of the effect of disruptions on heterologous laccase expression.Fungal Genet Biol,2008,45(1):17-27.
[58]Kiiskinen LL,Kruus K,Bailey M,et al.Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme.Microbiology,2004,150(9):3065-3074.
[59]Zhang JW,Qu YB,Xiao P,et al.Improved biomass saccharification by Trichoderma reesei through heterologous expression of lac A gene from Trametes sp.AH28-2.J Biosci Bioeng,2012,113(6):697-703.
[60]Dong XR,Qin LN,Tao Y,et al.Overexpression and characterization of a laccase gene from Pleurotus ostreatus in Trichoderma reesei.Acta Microbiol Sin,2012,52(7):850-856(in Chinese).董欣睿,秦丼娜,陶勇,等.糙皮侧耳(Pleurotus ostreatus)漆酶POXA1在里氏木霉中的高效表达及酶学性质.微生物学报,2012,52(7):850-856.
[61]Abianova AR,Chulkin AM,Vavilova EA,et al.Aheterologous production of the Trametes hirsuta laccase in the fungus Penicillium canescens.Prikl Biokhim Mikrobiol,2010,46(3):342-347.
[62]Fujihiro S,Higuchi R,Hisamatsu S,et al.Metabolism of hydroxylated PCB congeners by cloned laccase isoforms.Appl Microbiol Biotechnol,2009,82(5):853-860.
[63]SkálováT,Dohnálek J,?stergaard LH,et al.The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases.J Mol Biol,2009,385(4):1165-1178.
[64]Andberg M,Hakulinen N,Auer S,et al.Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production,catalytic properties and structure.FEBS J,2009,276(21):6285-6300.
[65]Berka RM,Schneider P,Golightly EJ,et al.Characterization of the gene encoding an extracellular laccase of Myceliophthora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae.Appl Environ Microbiol,1997,63(8):3151-3157.
[66]Bukh C,Lund M,Bjerrum MJ.Kinetic studies on the reaction between Trametes villosa laccase and dioxygen.J Inorg Biochem,2006,100(9):1547-1557.
[67]Hakulinen N,Kruus K,Koivula A,et al.Acrystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase.Biochem Biophys Res Commun,2006,350(4):929-934.
[68]Sigoillot C,Camarero S,Vidal T,et al.Comparison of different fungal enzymes for bleaching high-quality paper pulps.J Biotechnol,2005,115(4):333-343.
[69]Ravalason H,Herpo?l-Gimbert I,Record E,et al.Fusion of a family 1 carbohydrate binding module of Aspergillus niger to the Pycnoporus cinnabarinus laccase for efficient softwood kraft pulp biobleaching.J Biotechnol,2009,142(3/4):220-226.
[70]Li L,Steffens JC.Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance.Planta,2002,215(2):239-247.
[71]Chiaiese P,Palomba F,Tatino F,et al.Engineered tobacco and microalgae secreting the fungal laccase POXA1b reduce phenol content in olive oil mill wastewater.Enzyme Microb Technol,2011,49(6/7):540-546.
[72]Cesarino I,Araújo P,Mayer JLS,et al.Expression of Sof LAC,a new laccase in sugarcane,restores lignin content but not S?G ratio of Arabidopsis lac17 mutant.J Exp Bot,2013,64(6):1769-1781.
[73]Arakane Y,Muthukrishnan S,Beeman RW,et al.Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning.Proc Natl Acad Sci USA,2005,102(32):11337-11342.
[74]Dittmer NT,Gorman MJ,Kanost MR.Characterization of endogenous and recombinant forms of laccase-2,a multicopper oxidase from the tobacco hornworm,Manduca sexta.Insect Biochem Mol Biol,2009,39(9):596-606.
[75]Coy MR,Salem TZ,Denton JS,et al.Phenol-oxidizing laccases from the termite gut.Insect Biochem Mol Biol,2010,40(10):723-732.
[76]Hong F,Meinander NQ,J?nsson LJ.Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris.Biotechnol Bioeng,2002,79(4):438-449.
[77]Guo M,Lu FP,Du LX,et al.Optimization of the expression of a laccase gene from Trametes versicolor in Pichia methanolica.Appl Microbiol Biotechnol,2006,71(6):848-852.
[78]Liu W,Chao Y,Liu S,et al.Molecular cloning and characterization of a laccase gene from the basidiomycete Fome lignosus and expression in Pichia pastoris.Appl Microbiol Biotechnol,2003,63(2):174-181.
[79]Rivera-Hoyos CM,Morales-álvarez ED,Poveda-Cuevas SA,et al.Computational analysis and low-scale constitutive expression of laccases synthetic genes Gl LCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris.PLo S ONE,2015,10(1):e0116524.
[80]Mate DM,Gonzalez-Perez D,Kittl R,et al.Functional expression of a blood tolerant laccase in Pichia pastoris.BMC Biotechnol,2013,13:38.
[81]Bohlin C,J?nsson LJ,Roth R,et al.Heterologous expression of Trametes versicolor laccase in Pichia pastoris and Aspergillus niger.Appl Biochem Biotechnol,2006,129(1/2/3):195-214.
[82]Brown MA,Zhao ZW,Mauk AG.Expression and characterization of a recombinant multi-copper oxidase:laccase IV from Trametes versicolor.Inorganica Chim Acta,2002,331(1):232-238.
[83]Guo M,Lu FP,Pu J,et al.Molecular cloning of the c DNA encoding laccase from Trametes versicolor and heterologous expression in Pichia methanolica.Appl Microbiol Biotechnol,2005,69(2):178-183.
[84]Hildén K,M?kel?MR,Lundell T,et al.Heterologous expression and structural characterization of two low p H laccases from a biopulping white-rot fungus Physisporinus rivulosus.Appl Microbiol Biotechnol,2013,97(4):1589-1599.
[85]Lin YQ,Zhang Z,Tian YS,et al.Purification and characterization of a novel laccase from Coprinus cinereus and decolorization of different chemically dyes.Mol Biol Rep,2013,40(2):1487-1494.
[86]Bulter T,Alcalde M,Sieber V,et al.Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution.Appl Environ Microbiol,2003,69(2):987-995.
[87]Festa G,Autore F,Fraternali F,et al.Development of new laccases by directed evolution:functional and computational analyses.Proteins Struct Funct Bioinform,2008,72(1):25-34.