一株天然气生物脱硫菌的分离纯化和生物学特性研究
|
摘要
从天然气净化厂污水池回流污泥中分离到一株硫杆菌菌株TYY-1,通过生理生化特性鉴定,该菌株可以确定为脱氮硫杆菌。从最适生长条件、生长曲线两个方面初步探讨了该菌株的生物学特性。结果表明,该菌株最适接种量为10%,生长温度为30℃,初始pH值为7.5。
Thiobacillus strain TYY-1 was isolated from the sewage of a natural gas purification plant.According to the analysis of physiological and biochemical measurements,the strain were identified as Thiobacillus denitrificans.The optimum growth conditions of TYY-1 strain were determined as follows:the optimum inoculation amount was 10%,the culture temperature was 30 ℃,and the initial pH value was 7.5.
Thiobacillus strain TYY-1 was isolated from the sewage of a natural gas purification plant.According to the analysis of physiological and biochemical measurements,the strain were identified as Thiobacillus denitrificans.The optimum growth conditions of TYY-1 strain were determined as follows:the optimum inoculation amount was 10%,the culture temperature was 30 ℃,and the initial pH value was 7.5.
引文
[1]徐波,何金龙,黄黎明,等.天然气生物脱硫技术及其研究进展[J].天然气工业,2013,33(1):116-121.
[2]徐瑛,孙永明,袁振宏,等.生物燃气生物脱硫技术研究进展[J].应用与环境生物学报,2014,20(2):328-335.
[3]汪家铭.Shell-Paques生物脱硫技术及其应用[J].化肥设计,2010,48(2):39-42.
[4]孙茹,何金龙,徐波,等.高效液相色谱法测定生物脱硫溶液中单质硫[J].石油与天然气化工,2015,44(6):101-104.
[5]SYED M A,HENSHAW P F.Effect of tube size on performance of a fixed film tubular bioreactor for conversion of hydrogen sulfide to elemental sulfur[J].Water Research,2003,37(8):1932-1938.
[6]SOROKIN D Y,MUNTYAN M S,PANTELEEVA A N,et al.Thioalkalivibrio sulfidiphilus sp.nov.,a haloalkaliphilic,sulfur-oxidizing gammaproteobacterium from alkaline habitats[J].International Journal of Systematic and Evolutionary Microbiology,2012,62(8):1884-1889.
[7]MUYZER G,SOROKIN D Y,MAVROMATIS K,et al.Complete genome sequence of“Thioalkalivibrio sulfidophilus”HL-EbGr7[J].Standard Genomic Sciences,2011,4(1):23-35.
[8]HOLE U H,VOLLACK K U,ZUMFT W G,et al.Characterization of the membranous denitrification enzymes nitrite reductase(cytochrome cd1)and copper-containing nitrous oxide reductase from Thiobacillus denitrificans[J].Archives of Microbiology,1996,165(1):55-61.
[9]万淑娟,党志,杨琛,等.一株氧化亚铁硫杆菌的筛选及生长条件研究[J].环境工程学报,2010,4(9):2146-2150.
[10]车轩,罗国芝,谭洪新,等.脱氮硫杆菌的分离鉴定和反硝化特性研究[J].环境科学,2008,29(10):2931-2937.
[2]徐瑛,孙永明,袁振宏,等.生物燃气生物脱硫技术研究进展[J].应用与环境生物学报,2014,20(2):328-335.
[3]汪家铭.Shell-Paques生物脱硫技术及其应用[J].化肥设计,2010,48(2):39-42.
[4]孙茹,何金龙,徐波,等.高效液相色谱法测定生物脱硫溶液中单质硫[J].石油与天然气化工,2015,44(6):101-104.
[5]SYED M A,HENSHAW P F.Effect of tube size on performance of a fixed film tubular bioreactor for conversion of hydrogen sulfide to elemental sulfur[J].Water Research,2003,37(8):1932-1938.
[6]SOROKIN D Y,MUNTYAN M S,PANTELEEVA A N,et al.Thioalkalivibrio sulfidiphilus sp.nov.,a haloalkaliphilic,sulfur-oxidizing gammaproteobacterium from alkaline habitats[J].International Journal of Systematic and Evolutionary Microbiology,2012,62(8):1884-1889.
[7]MUYZER G,SOROKIN D Y,MAVROMATIS K,et al.Complete genome sequence of“Thioalkalivibrio sulfidophilus”HL-EbGr7[J].Standard Genomic Sciences,2011,4(1):23-35.
[8]HOLE U H,VOLLACK K U,ZUMFT W G,et al.Characterization of the membranous denitrification enzymes nitrite reductase(cytochrome cd1)and copper-containing nitrous oxide reductase from Thiobacillus denitrificans[J].Archives of Microbiology,1996,165(1):55-61.
[9]万淑娟,党志,杨琛,等.一株氧化亚铁硫杆菌的筛选及生长条件研究[J].环境工程学报,2010,4(9):2146-2150.
[10]车轩,罗国芝,谭洪新,等.脱氮硫杆菌的分离鉴定和反硝化特性研究[J].环境科学,2008,29(10):2931-2937.