磁纳米颗粒固定化多能硫碱弧菌(T.versutus D301)生物脱硫
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摘要
采用共沉淀法合成油酸修饰的Fe3O4纳米颗粒,用其固定化Thioalkalivibrio versutus D301细胞,考察了盐碱环境下纳米颗粒固定化细胞的工艺条件,比较了固定化细胞和游离细胞的硫氧化活性,研究了固定化细胞的重复利用性能.结果表明,最佳固定化条件为Na+浓度0.6 mol/L,p H值9.5,固定化温度20℃,吸附时间10 min.固定化细胞硫氧化速率是游离细胞的81%.固定化细胞具有很好的硫氧化活性,可重复使用至少6次.
Magnetic Fe3O4 nanoparticles were synthesized by a coprecipitation method followed by modification with oleic acid, which was used for immobilizing Thioalkalivibrio versutus D301 cells. The process parameters of cell immobilization under halo-alkaline condition, the sulfur oxidation rate of free cells and immobilized cells and recycling ability of immobilized cells were investigated. The results indicated that the optimum immobilization conditions were that the cells were immobilized for 10 min at the conditions of 0.6 mol/L Na+, p H 9.5 and 20 ℃. The sulfur oxidation rate of immobilized cells was 81% of free cells after activation. The coated cells not only had good sulfur oxidation activity, but also could be reused six batch cycles.
Magnetic Fe3O4 nanoparticles were synthesized by a coprecipitation method followed by modification with oleic acid, which was used for immobilizing Thioalkalivibrio versutus D301 cells. The process parameters of cell immobilization under halo-alkaline condition, the sulfur oxidation rate of free cells and immobilized cells and recycling ability of immobilized cells were investigated. The results indicated that the optimum immobilization conditions were that the cells were immobilized for 10 min at the conditions of 0.6 mol/L Na+, p H 9.5 and 20 ℃. The sulfur oxidation rate of immobilized cells was 81% of free cells after activation. The coated cells not only had good sulfur oxidation activity, but also could be reused six batch cycles.
引文
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[3]De Graaff M,Klok J B,Bijmans M F,et al.Application of a 2-Step Process for the Biological Treatment of Sulfidic Spent Caustics[J].Water Res.,2012,46(3):723-730.
[4]Sorokin D Y,Lysenko A M,Mityushina L L,et al.Thioalkalimicrobium aerophilum gen.nov.,sp.nov.and Thioalkalimicrobium sibericum sp.nov.,and Thioalkalivibrio versutus gen.nov.,sp.nov.,Thioalkalivibrio nitratis sp.nov.,Novel and Thioalkalivibrio denitrificancs sp.nov.,Novel Obligately Alkaliphilic and Obligately Chemolithoautotrophic Sulfur-oxidizing Bacteria from Soda Lakes[J].Int.J.Syst.Evol.Microbiol.,2001,51(2):565-580.
[5]Wang J L,Quan X C,Han L P,et al.Microbial Degradation of Quinoline by Immobilized Cells of Burkholderia pickettii[J].Water Res.,2002,36(9):2288-2296.
[6]Wang L,Han L P,Shi H C,et al.Biodegradation of Quinoline by Gel Immobilized Burkholderia sp.[J].Chemosphere,2001,44(5):1041-1046.
[7]Xu L,Yang L,Luo M,et al.Reduction of Hexavalent Chromium by Pannonibacter phragmitetus LSSE-09 Coated with Polyethylenimine-functionalized Magnetic Nanoparticles under Alkaline Conditions[J].J.Hazard.Mater.,2011,189(3):787-793.
[8]Li Y G,Gao H S,Li W L,et al.In situ Magnetic Separation and Immobilization of Dibenzothiophene-desulfurizing Bacteria[J].Bioresour.Technol.,2009,100(21):5092-5096.
[9]Pfenning N,Lippert K.Vitamin B12-bedürfnis Photototropher Schwefelbakterien[J].Arch.Microbiol.,1966,55:245-256.
[10]Zhou J M,Song Z Y,Yan D J,et al.Performance of a Haloalkaliphilic Bioreactor and Bacterial Community Shifts under Different COD/SO42-Ratios and Hydraulic Retention Times[J].J.Hazard.Mater.,2014,274:53-62.
[11]Lee J,Isobe T,Senna M.Preparation of Ultrafine Fe3O4 Particles by Precipitation in the Presence of PVA at High p H[J].J.Colloid Interface Sci.,1996,177(2):490-494.
[12]El-Hilo M,O'grady K,Chantrell R.Susceptibility Phenomena in a Fine Particle System:I.Concentration Dependence of the Peak[J].J.Magn.Magn.Mater.,1992,114(3):295-306.
[13]Liu X,Kaminski M D,Guan Y,et al.Preparation and Characterization of Hydrophobic Superparamagnetic Magnetite Gel[J].J.Magn.Magn.Mater.,2006,306(2):248-253.
[14]Korolev V,Ramazanova A,Blinov A.Adsorption of Surfactants on Superfine Magnetite[J].Russ.Chem.Bull.,2002,51(11):2044-2049.
[15]Petrache H I,Zemb T,Belloni L,et al.Salt Screening and Specific Ion Adsorption Determine Neutral-lipid Membrane Interactions[J].Proc.Natl.Acad.Sci.USA,2006,103(21):7982-7987.
[16]Hu Y R,Wang F,Wang S K,et al.Efficient Harvesting of Marine Microalgae Nannochloropsis maritima Using Magnetic Nanoparticles[J].Bioresour.Technol.,2013,138:387-390.
[17]Nassar N N.Rapid Removal and Recovery of Pb(II)from Wastewater by Magnetic Nanoadsorbents[J].J.Hazard.Mater.,2010,184(1/3):538-546.
[18]Aravindhan R,Rao J R,Nair B U.Removal of Basic Yellow Dye from Aqueous Solution by Sorption on Green Alga Caulerpa scalpelliformis[J].J.Hazard.Mater.,2007,142(1/2):68-76.
[19]Pan Y,Neuss S,Leifert A,et al.Size-dependent Cytotoxicity of Gold Nanoparticles[J].Small,2007,3(11):1941-1949.
[20]Lyon D Y,Alvarez P J.Fullerene Water Suspension(n C60)Exerts Antibacterial Effects via ROS-independent Protein Oxidation[J].Environ.Sci.Technol.,2008,42(18):8127-8132.
[21]Liu J F,Zhao Z S,Jiang G B.Coating Fe3O4 Magnetic Nanoparticles with Humic Acid for High Efficient Removal of Heavy Metals in Water[J].Environ.Sci.Technol.,2008,42(18):6949-6954.
[22]Zhang L,Li Y,Jimmy C Y,et al.Assembly of Polyethylenimine-functionalized Iron Oxide Nanoparticles as Agents for DNA Transfection with Magnetofection Technique[J].J.Mater.Chem.B,2014,2(45):7936-7944.
[23]Xia T,Guan Y,Yang M,et al.Synthesis of Polyethylenimine Modified Fe3O4 Nanoparticles with Immobilized Cu2+for Highly Efficient Proteins Adsorption[J].Colloids Surf.A,2014,443:552-559.
[24]Clejan S,Krulwich T A,Mondrus K R,et al.Membrane Lipid Composition of Obligately and Facultatively Alkalophilic Strains of Bacillus spp[J].J.Bacteriol.,1986,168(1):334-340.
[25]Shan G,Xing J,Zhang H,et al.Biodesulfurization of Dibenzothiophene by Microbial Cells Coated with Magnetite Nanoparticles[J].Appl.Environ.Microbiol.,2005,71(8):4497-4502.
[26]Zhang W,Rittmann B,Chen Y.Size Effects on Adsorption of Hematite Nanoparticles on E.coli Cells[J].Environ.Sci.Technol.,2011,45(6):2172-2178.