PP333和TIBA对大叶黄杨根系形态及根系活力的影响
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摘要
采用双因素随机区组设计,以绿篱植物大叶黄杨为试验材料,研究不同浓度PP333、TIBA对其根系形态及活力的影响,探讨化学修剪对绿蓠植物的养分吸收机理。结果表明:(1)单施PP333极显著提高了大叶黄杨根系形态、细根根系的形态、根系活力,且随着PP333浓度升高而增大。单施TIBA仅对根平均直径、根表面积、细根的各根系形态指标以及活跃吸收面积有显著促进作用,且均在TIBA50 mg/L时达到最大值;(2)PP333和TIBA的交互作用对根体积、细根根系形态以及活跃吸收面积、活跃比表面积有显著促进作用,其中根系体积、细根根系的根长和根体积在80 mg/LPP333与100 mg/LTIBA配合喷施下达到最大值,而细根根系的表面积、根系活跃吸收面积和活跃比表面积则在80 mg/LPP333与50 mg/LTIBA配合喷施下达到最大值。PP333和TIBA通过对细根形态的影响,显著提高了根系的吸收活力。
In this study, Euonymus japonicus was used as the test material and two-factor completely randomize design was adopted to investigate the effects of PP333 and TIBA in different concentration levels on root morphology and root activity of Euonymus japonicus, aimed at providing the theoretical basis for mechanism of effects of chemical pruning on nutrient uptake and utilization of hedge plants.Results indicated that:(1)all root morphological characteristics,root activity indexes and morphological characteristics of fine root were promoted extremely significantly by applying PP333 individually, the data of which increased with higher concentration of PP333; Average root diameter, root surface area, morphological characteristics of fine root and active absorption were favored significantly in different extents by applying TIBA individually. Also, the data reached the top in low concentration of TIBA;(2)Root volume, morphological characteristics, active absorption area and active specific surface area were improved significantly under the interaction of PP333 and TIBA.The data of root volume, root length and volume of fine root reached the peak at treatment of combination of high concentration of PP333(80 mg/L) and high concentration of TIBA(100 mg/L). Differently, the number of root surface of fine root, active absorption area and active specific surface area reached the top at treatment of combination of high concentration of PP333(80 mg/L) and low concentration of TIBA(50 mg/L). Overall, Root morphology and root activity of Euonymus japonicus had a significant plasticity under the main effects and interaction of PP333 and TIBA.And PP333 and TIBA improve the root activity of Euonymus japonicus significantly by influencing fine root morphology.
In this study, Euonymus japonicus was used as the test material and two-factor completely randomize design was adopted to investigate the effects of PP333 and TIBA in different concentration levels on root morphology and root activity of Euonymus japonicus, aimed at providing the theoretical basis for mechanism of effects of chemical pruning on nutrient uptake and utilization of hedge plants.Results indicated that:(1)all root morphological characteristics,root activity indexes and morphological characteristics of fine root were promoted extremely significantly by applying PP333 individually, the data of which increased with higher concentration of PP333; Average root diameter, root surface area, morphological characteristics of fine root and active absorption were favored significantly in different extents by applying TIBA individually. Also, the data reached the top in low concentration of TIBA;(2)Root volume, morphological characteristics, active absorption area and active specific surface area were improved significantly under the interaction of PP333 and TIBA.The data of root volume, root length and volume of fine root reached the peak at treatment of combination of high concentration of PP333(80 mg/L) and high concentration of TIBA(100 mg/L). Differently, the number of root surface of fine root, active absorption area and active specific surface area reached the top at treatment of combination of high concentration of PP333(80 mg/L) and low concentration of TIBA(50 mg/L). Overall, Root morphology and root activity of Euonymus japonicus had a significant plasticity under the main effects and interaction of PP333 and TIBA.And PP333 and TIBA improve the root activity of Euonymus japonicus significantly by influencing fine root morphology.
引文
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[3]李德燕,周运超.马尾松幼苗生长对钙浓度的响应[J].中南林业科技大学学报,2017,27(12):39-45.
[4]李千惠,范俊俊,赵明明,等.外源激素在金雀花扦插生根进程中的调节机制[J].中南林业科技大学学报,2017,37(10):54-60.
[5]何应会,蒋燚,姜英,等.NAA与切根组合对江南油杉苗内源激素及根系构型的调节效应[J].中南林业科技大学学报,2017,37(8):11-17.
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[7]黄旭光,罗恩波,陆仟,等.多效唑与矮壮素对福建茶的矮化效果[J].南方农业学报,2012,43(10):1549-1552.
[8]LAUBSCHERC P,NDAKIDEMI P A,et al.Paclobutrazol retards vegetative growth in hydroponically-cultured Leonotis leonurus(L.)R.Br.Lamiaceae,for a multipurpose flowering potted plant[J].South African Journal of Botany,2016,106(5):67-70.
[9]ZHANG L,YAN P,Shen C,et al.Effects of exogenous TIBA on dwarfing,shoot branching and yield of tea plant(Camellia sinensis L.)[J].Scientia Horticulturae,2017,225(10):676-680.
[10]TANTASAWAT P A,SORNTIP A,PORNBUNGKERD P.Effects of exogenous application of plant growth regulators on growth,yield,and in vitro gynogenesis in cucumber[J].Hortscience,2015,50(3):374-382.
[11]ZHANG S,ZHANG D,FAN S,et al.Effect of exogenous GA3and its inhibitor paclobutrazol on floral formation,endogenous hormones,and flowering-associated genes in‘Fuji’apple(Malus domestica Borkh.)[J].Plant Physiology&Biochemistry,2016,107(6):178-186.
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[13]CHUSRI O,KOZAI N,OGATA T,et al.Application of Paclobutrazol for Flowering and Fruit Production of‘Irwin’Mango(Mangifera indica L.)in Okinawa[J].International Journal of Life Cycle Assessment,2009,12(2):103-108.
[14]PADILLA I M G,FERNáNDEZ-GARCíA N,OLMOS E,et al.Effects of growth retardants on sprouting and development of apricot(Prunus armeniaca L.)and neem(Azarchta indica A.Juss.)nodal buds[J].Plant Cell Tissue&Organ Culture,2015,122(2):285-297.
[15]SAWAN Z M.Cottonseed yield and its quality as affected by mineral fertilizers and plant growth retardants[J].Agricultural Science,2014,5(3):186-209.
[16]JUNGKLANG J,SAENGNIL K.Effect of paclobutrazol on patumma cv.Chiang Mai Pink under water stress[J].Songklanakarin Journal of Science&Technology,2012,34(4):361-366.
[17]李金航,齐秀慧,徐程扬,等.华北4产地黄栌幼苗根系形态对干旱胁迫的短期响应[J].北京林业大学学报,2014,36(1):48-54.
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[19]MCCORMACK M L,DICKIE I A,EISSENSTAT D M,et al.Redefining fine roots improves understanding of belowground contributions to terrestrial biosphere processes[J].New Phytologist,2015,39(8):505-518.
[20]张伟明,孟军,王嘉宇,等.生物炭对水稻根系形态与生理特性及产量的影响[J].作物学报,2013,39(8):1445-1451.
[21]庞世龙,欧芷阳,申文辉,等.干旱胁迫对蚬木幼苗表型可塑性的影响[J].中南林业科技大学学报,2017,37(5):21-25.
[22]林多,赵康,陈宁,等.植物生长延缓剂对夏季番茄穴盘苗生长及根系形态的影响[J].西北农业学报,2013,22(10):140-145.
[23]白小明,相斐,罗仁峰.多效唑对高羊茅扩展性和根系特性的调控效应[J].草业科学,2009,26(10):171-176.
[24]王宇灵,白小明,罗仁峰.多效唑对多年生黑麦草扩展性和根系特性的影响[J].中国沙漠,2010,30(6):1319-1324.
[25]王义强,王启业,华连滩,等.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响[J].中南林业科技大学学报,2017,37(10):7-12.
[26]PACURAR D I,PACURAR M L,BUSSELL J D,et al.Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot 2 mutation[J].Journal of Experimental Botany,2014,65(6):1605.
[27]AMIJIMA M,IWATA Y,KOIZUMI N,et al.The polar auxin transport inhibitor TIBA inhibits endore duplication in dark grown spinach hypocotyls[J].Plant Science,2014,225(8):45-51.
[28]WANG Y C,MENG Y,WAN-RONG G U,et al.Effects of Mixed Compound of DCPTA and CCC on Root Growth and Stem Agronomic Traits of Spring Maize in Cold Area[J].Acta Agriculture Boreali Sinica,2014,29(2):156-160.
[29]陈冠陶,郑军,彭天驰,等.扁刺栲不同根序细根形态和化学特征及其对短期氮添加的响应[J].应用生态学报,2017(8):1-11.
[30]陈晓娜,高永,党晓宏,等.植物延缓剂对盐碱胁迫下羊柴幼苗根系形态的影响[J].水土保持研究,2017,24(4):187-190.
[31]匡冬姣,雷丕锋.不同林龄杉木人工林细根生物量及分布特征[J].中南林业科技大学学报,2015,35(6):70-74.
[32]江俐妮,魏红旭,刘勇,等.长白落叶松播种苗根系形态可塑性与氮素空间异质性关系[J].东北林业大学学报,2010,38(1):24-27.
[33]Adans T S,Mccormack M L,Eissenstat D M.Foraging strategies in trees of different root morphology:The role of root lifespan[J].Tree Physiology,2013,33:940-948.
[34]张静,程智慧,孟焕文.多效唑包衣处理对番茄种子活力和幼苗质量的影响[J].西北农林科技大学学报(自然科学版),2007,35(9):161-166.
[35]LU S N,CHE W G,ZHOU Y F,et al.Inhibition of Sb ABI5expression in roots by ultra-high endogenous ABA accumulation results in sorghum sensitivity to salt stress[J].International Journal of Agriculture&Biology,2016,18(1):146-154.
[36]贾全全,刘琪璟,梁宇.三种常见针叶树种的细根形态比较[J].中南林业科技大学学报,2016,36(2):33-39.
[37]范伟国,杨洪强.平邑甜茶根系构型、养分吸收和新梢生长对根域形状的反应[J].中国农业科学,2014,47(19):3907-3913.
[38]薛艳.植物生长延缓剂对不同作物的作用及其机理研究[D].武汉:华中农业大学,2014.
[2]PESTOVA T V,BORUKHOV S I,HELLEN C U.Eukaryotic ribosomes require initiation factors 1 and 1A to locate initiation codons[J].Nature,1998,394(8):854-859.
[3]李德燕,周运超.马尾松幼苗生长对钙浓度的响应[J].中南林业科技大学学报,2017,27(12):39-45.
[4]李千惠,范俊俊,赵明明,等.外源激素在金雀花扦插生根进程中的调节机制[J].中南林业科技大学学报,2017,37(10):54-60.
[5]何应会,蒋燚,姜英,等.NAA与切根组合对江南油杉苗内源激素及根系构型的调节效应[J].中南林业科技大学学报,2017,37(8):11-17.
[6]NAIDU J H,ASHOK P,SEKHAR R C,et al.Effect of plant growth retardants and spacings on vegetative growth and flower yield of African marigold(Tagetes erecta L)cv.Pusa Narangi Gainda[J].International Journal of Farm Sciences,2014,4(2):92-99.
[7]黄旭光,罗恩波,陆仟,等.多效唑与矮壮素对福建茶的矮化效果[J].南方农业学报,2012,43(10):1549-1552.
[8]LAUBSCHERC P,NDAKIDEMI P A,et al.Paclobutrazol retards vegetative growth in hydroponically-cultured Leonotis leonurus(L.)R.Br.Lamiaceae,for a multipurpose flowering potted plant[J].South African Journal of Botany,2016,106(5):67-70.
[9]ZHANG L,YAN P,Shen C,et al.Effects of exogenous TIBA on dwarfing,shoot branching and yield of tea plant(Camellia sinensis L.)[J].Scientia Horticulturae,2017,225(10):676-680.
[10]TANTASAWAT P A,SORNTIP A,PORNBUNGKERD P.Effects of exogenous application of plant growth regulators on growth,yield,and in vitro gynogenesis in cucumber[J].Hortscience,2015,50(3):374-382.
[11]ZHANG S,ZHANG D,FAN S,et al.Effect of exogenous GA3and its inhibitor paclobutrazol on floral formation,endogenous hormones,and flowering-associated genes in‘Fuji’apple(Malus domestica Borkh.)[J].Plant Physiology&Biochemistry,2016,107(6):178-186.
[12]WILHELM,HANSJOERG F,GRAEBE J E,et al.Tetcyclacis and triazole type plant growth retardants:Their influence on the biosynthesis of gibberellins and other metabolic processes[J].Pest Management Science,2010,21(4):241-252.
[13]CHUSRI O,KOZAI N,OGATA T,et al.Application of Paclobutrazol for Flowering and Fruit Production of‘Irwin’Mango(Mangifera indica L.)in Okinawa[J].International Journal of Life Cycle Assessment,2009,12(2):103-108.
[14]PADILLA I M G,FERNáNDEZ-GARCíA N,OLMOS E,et al.Effects of growth retardants on sprouting and development of apricot(Prunus armeniaca L.)and neem(Azarchta indica A.Juss.)nodal buds[J].Plant Cell Tissue&Organ Culture,2015,122(2):285-297.
[15]SAWAN Z M.Cottonseed yield and its quality as affected by mineral fertilizers and plant growth retardants[J].Agricultural Science,2014,5(3):186-209.
[16]JUNGKLANG J,SAENGNIL K.Effect of paclobutrazol on patumma cv.Chiang Mai Pink under water stress[J].Songklanakarin Journal of Science&Technology,2012,34(4):361-366.
[17]李金航,齐秀慧,徐程扬,等.华北4产地黄栌幼苗根系形态对干旱胁迫的短期响应[J].北京林业大学学报,2014,36(1):48-54.
[18]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-261.
[19]MCCORMACK M L,DICKIE I A,EISSENSTAT D M,et al.Redefining fine roots improves understanding of belowground contributions to terrestrial biosphere processes[J].New Phytologist,2015,39(8):505-518.
[20]张伟明,孟军,王嘉宇,等.生物炭对水稻根系形态与生理特性及产量的影响[J].作物学报,2013,39(8):1445-1451.
[21]庞世龙,欧芷阳,申文辉,等.干旱胁迫对蚬木幼苗表型可塑性的影响[J].中南林业科技大学学报,2017,37(5):21-25.
[22]林多,赵康,陈宁,等.植物生长延缓剂对夏季番茄穴盘苗生长及根系形态的影响[J].西北农业学报,2013,22(10):140-145.
[23]白小明,相斐,罗仁峰.多效唑对高羊茅扩展性和根系特性的调控效应[J].草业科学,2009,26(10):171-176.
[24]王宇灵,白小明,罗仁峰.多效唑对多年生黑麦草扩展性和根系特性的影响[J].中国沙漠,2010,30(6):1319-1324.
[25]王义强,王启业,华连滩,等.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响[J].中南林业科技大学学报,2017,37(10):7-12.
[26]PACURAR D I,PACURAR M L,BUSSELL J D,et al.Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot 2 mutation[J].Journal of Experimental Botany,2014,65(6):1605.
[27]AMIJIMA M,IWATA Y,KOIZUMI N,et al.The polar auxin transport inhibitor TIBA inhibits endore duplication in dark grown spinach hypocotyls[J].Plant Science,2014,225(8):45-51.
[28]WANG Y C,MENG Y,WAN-RONG G U,et al.Effects of Mixed Compound of DCPTA and CCC on Root Growth and Stem Agronomic Traits of Spring Maize in Cold Area[J].Acta Agriculture Boreali Sinica,2014,29(2):156-160.
[29]陈冠陶,郑军,彭天驰,等.扁刺栲不同根序细根形态和化学特征及其对短期氮添加的响应[J].应用生态学报,2017(8):1-11.
[30]陈晓娜,高永,党晓宏,等.植物延缓剂对盐碱胁迫下羊柴幼苗根系形态的影响[J].水土保持研究,2017,24(4):187-190.
[31]匡冬姣,雷丕锋.不同林龄杉木人工林细根生物量及分布特征[J].中南林业科技大学学报,2015,35(6):70-74.
[32]江俐妮,魏红旭,刘勇,等.长白落叶松播种苗根系形态可塑性与氮素空间异质性关系[J].东北林业大学学报,2010,38(1):24-27.
[33]Adans T S,Mccormack M L,Eissenstat D M.Foraging strategies in trees of different root morphology:The role of root lifespan[J].Tree Physiology,2013,33:940-948.
[34]张静,程智慧,孟焕文.多效唑包衣处理对番茄种子活力和幼苗质量的影响[J].西北农林科技大学学报(自然科学版),2007,35(9):161-166.
[35]LU S N,CHE W G,ZHOU Y F,et al.Inhibition of Sb ABI5expression in roots by ultra-high endogenous ABA accumulation results in sorghum sensitivity to salt stress[J].International Journal of Agriculture&Biology,2016,18(1):146-154.
[36]贾全全,刘琪璟,梁宇.三种常见针叶树种的细根形态比较[J].中南林业科技大学学报,2016,36(2):33-39.
[37]范伟国,杨洪强.平邑甜茶根系构型、养分吸收和新梢生长对根域形状的反应[J].中国农业科学,2014,47(19):3907-3913.
[38]薛艳.植物生长延缓剂对不同作物的作用及其机理研究[D].武汉:华中农业大学,2014.