细胞共培养技术在骨组织生物学中的应用
|
摘要
骨组织不同细胞相互交织构成了复杂的网络结构,通过旁分泌和间隙连接途径相互传递信号,调节了个体发育的骨重塑和成体的骨重建过程。细胞共培养技术是研究细胞间相互作用的重要手段,目前主要有接触式和非接触式两种。就骨组织而言,由于骨细胞(osteocyte)在力学感知及调节骨重塑/重建平衡中的中心枢纽作用,建立适宜于骨细胞与骨组织其他细胞间的共培养技术,对于骨组织基础生物学的研究具有重要意义。该文就当前应用于骨组织生物学中的细胞共培养技术进行综述。
Different bone cells constitutes a complex network structure,which transmit signals each other via paracrine and gap junction channels and adjust bone modeling and remodeling.Cells co-culture technique is the key method to investigate cell/cell interactions in vitro.Currently,this technique has two types: the contact coculture and non-contact co-culture.For the bone tissue,since the importance of osteocyte in mechanical sensing and in regulation of bone modeling and remodeling,it is important for the research of bone biology to establish the suitable co-culture technique between osteocyte and other bone cells.This article is aimed at reviewing the development of cells co-culture technique in the bone tissue biology.
Different bone cells constitutes a complex network structure,which transmit signals each other via paracrine and gap junction channels and adjust bone modeling and remodeling.Cells co-culture technique is the key method to investigate cell/cell interactions in vitro.Currently,this technique has two types: the contact coculture and non-contact co-culture.For the bone tissue,since the importance of osteocyte in mechanical sensing and in regulation of bone modeling and remodeling,it is important for the research of bone biology to establish the suitable co-culture technique between osteocyte and other bone cells.This article is aimed at reviewing the development of cells co-culture technique in the bone tissue biology.
引文
1 Bonewald LF.Mechanosensation and transduction in osteocytes.Bonekey Osteovision 2006;3(10):7-15.
2 Gusm o CV,Belangero WD.How do bone cells sense mechani-cal loading.Rev Bras Ortop 2009;44(4):299-305.
3骞爱荣,胡丽芳,狄升蒙,高翔,孟芮,商澎.骨细胞在力学信号感受过程中的作用.中华航空航天医学杂志(Qian Ai-rong,Hu Lifang,Di Shengmeng,Gao Xiang,Meng Rui,ShangPeng.Roles of osteocytes in mechanosensation.Chin J Aero-space Med)2010;21(2):149-53.
4 Cole RJ,Paul J.Properties of cultured preimplantation mouseand rabbitembryos,and cell strains derived from them.CibaFoundation Symposium-Preimplantation Stages of Pregnancy1965;82-112.
5 James Kirkpatrick C,Fuchs S,Iris Hermanns M,Peters K,UngerRE.Cell culture models of higher complexity in tissue engineer-ing and regenerative medicine.Biomaterials 2007;28(34):5193-8.
6 Wang J,Ren L,Li L,Liu W,Zhou J,Yu W,et al.Microfluidics:A new cosset for neurobiology.Lab Chip 2009;9(5):644-52.
7 Peyrin JM,Deleglise B,Saias L,Vignes M,Gougis P,Magnifico S,etal.Axon diodes for the reconstruction of oriented neuronal net-works in microfluidic chambers.Lab Chip 2011;21(11):3663-73.
8Mow VC,Huiskes R,主编.骨科生物力学暨力学生物学,第3版.汤亭亭,裴国献,李旭,白波,主译.山东:山东科学技术出版社(Mow VC,Huiskes R.Basic Orthopaedic Biomechan-ics and Mechano-Biology,3rd ed.Tang Tingting,Pei Guoxian,Li Xu,Bai Bo,Translate.Shandong:Shangdong Scientific andTechnical Publishers),2009,12-6.
9蒋斯,韩泽民.引导骨组织再生技术在牙槽骨植骨中的应用现状.武警医学院学报(Jiang Si,Han Zeming.Current situationon the clinical application of alveolar augmentation combine withguided bone regeneration.Acta Academiae Medicinae CPAF)2011;20(8):686-8.
10 Riggs BL,Melton LJ 3rd.Evidence for two distinct syndromesof involutional osteoporosis.Am J Med 1983;75(6):899-901.
11 Dempster DW,Lambing CL,Kostenuik PJ,Grauer A.Role ofRANK ligand and denosumab,a targeted RANK ligand inhibi-tor,in bone health and osteoporosis:A review of preclinical andclinical data.Clin Ther 2012;34(3):521-36.
12丁冲,杨宪园,杨鹏飞,商澎.关于机械振动对抗骨量丢失的研究进展.中华物理医学与康复杂志(Ding Chong,Yang Xi-anyuan,Yang Pengfei,Shang Peng.Advance in countermeasureagainst bone loss of vibration.Chin J Phys Med Rehabil)2010;32(10):795-7.
13丁冲,杨鹏飞,商澎.用于预防和治疗骨丢失的机械振动装置.北京生物医学工程(Ding Chong,Yang Pengfei,ShangPeng.A mechanical vibration device for preventing and treatingbone loss.Beijing Biomedical Engineering)2009;28(2):165-9.
14 Bonewald LF.The amazing osteocyte.J Bone Miner Res 2011;26(2):229-38.
15 Pead MJ,Suswillo R,Skerry TM,Vedi S,Lanyon LE.Increased3H-uridine levels in osteocytes following a single short periodof dynamic bone loading in vivo.Calcif Tissue Int 1988;43(2):92-6.
16 Kamioka H,Honjo T,Takano-Yamamoto T.A three-dimensionaldistribution of osteocyte processes revealed by the combinationof confocal laser scanning microscopy and differential interfer-ence contrast microscopy.Bone 2001;28(2):145-9.
17 Kamioka H,Sugawara Y,Honjo T,Yamashiro T,Takano-Yama-moto T.Terminal differentiation of osteoblasts to osteocytes isaccompanied by dramatic changes in the distribution of actin-binding proteins.J Bone Miner Res 2004;19(3):471-8.
18 Cheng B,Zhao S,Luo J,Sprague E,Bonewald LF,Jiang JX.Ex-pression of functional gap junctions and regulation by fluid flowin osteocyte-like MLO-Y4 cells.J Bone Miner Res 2001;16(2):249-59.
19 Takai E,Mauck RL,Hung CT,Guo XE.Osteocyte viability andregulation of osteoblast function in a 3D trabecular bone explantunder dynamic hydrostatic pressure.J Bone Miner Res 2004;19(9):1403-10.
20 Taylor AF,Saunders MM,Shingle DL,Cimbala JM,Zhou Z,Donahue HJ.Mechanically stimulated osteocytes regulate os-teoblastic activity via gap junctions.Am J Physiol Cell Physiol2007;292(2):545-52.
21 Chan ME,Lu XL,Huo B,Baik AD,Chiang V,Guldberg RE,etal.A trabecular bone explant model of osteocyte-osteoblast co-culture for bone mechanobiology.Cell Mol Bioeng 2009;2(3):405-15.
22 Adachi T,Aonuma Y,Taira K,Hojo M,Kamioka H.Asymmetricintercellular communication between bone bells:propagation ofthe calcium sigmaling.Biochem Biophys Res Commun 2009;389(3):495-500.
23 Kurata K,Heino TJ,Higaki H,V n nen HK.Bone marrow celldifferentiation induced by mechanically damaged osteocytes in3D gel-embedded culture.J Bone Miner Res 2006;21(4):616-25.
24 Yellowley CE,Li Z,Zhou Z,Jacobs CR,Donahue HJ.Functionalgap junctions between osteocytic and osteoblastic cells.J BoneMiner Res 2000;15(2):209-17.
25 Vatsa A,Smit TH,Klein-Nulend J.Extracellular NO signallingfrom a mechanically stimulated osteocyte.J Biomech 2007;40Suppl 1:S89-95.
26 Zhao S,Zhang YK,Harris S,Ahuja SS,Bonewald LF.MLO-Y4osteocyte-like cells support osteoclast formation and activation.JBone Miner Res 2002;17(11):2068-79.
27 Yaccoby S,Wezeman MJ,Zangari M,Walker R,Cottler-Fox M,Gaddy D,et al.Inhibitory effects of osteoblasts and increasedbone formation on myeloma in novel culture systems and a my-elomatous mouse model.Haematologica 2006;91(2):192-9.
28 Zhou H,Mak W,Zheng Y,Dunstan CR,Seibel MJ.Osteoblastsdirectly control lineage commitment of mesenchymal progenitorcells through Wnt signaling.J Biol Chem 2008;283(4):1936-45.
29 Wang IE,Shan J,Choi R,Oh S,Kepler CK,Chen FH,et al.Role of osteoblast–fibroblast interactions in the formation of theligament-to-bone interface.J Orthop Res 2007;25(12):1609-20.
30 Boggs ME,Thompson WR,Farach-Carson MC,Duncan RL,Beebe TP.Co-culture of osteocytes and neurons on a unique pat-terned surface.Biointerphases 2011;6(4):200-9.
31 Masuyama R,Stockmans I,Torrekens S,van Looveren R,MaesC,Carmeliet P,et al.Vitamin D receptor in chondrocytes pro-motes osteoclastogenesis and regulates FGF23 production inosteoblasts.J Clin Invest 2006;116(12):3150-9.
32孟芮,罗明志,王亮,高翔,狄升蒙,商澎.三维回转骨细胞条件培养基对成骨细胞功能的调节作用.中国生物化学与分子生物学报(Meng Rui,Luo Mingzhi,Wang Liang,Gao Xi-ang,Di Shengmeng,Shang Peng.Regulating role of conditionedmedium of osteocytes subjected to 3-D rotation on functions ofosteoblasts.Chinese Journal of Biochemistry and Molecular Bi-ology)2010;26(2):95-102.
33孟芮,续惠云,王哲,张维,商澎.模拟失重骨细胞条件培养基对成骨细胞活性的影响.航天医学与医学工程(MengRui,Xu Huiyun,Wang Zhe,Zhang Wei,Shang Peng.Effects ofconditioned meduim of osteocytes subjeced to simulated weight-lessness on activity of osteoblasts.Space Medicine&MedicalEngineering)2009;22(4):247-51.
34杨德洪,金大地,陈建庭,武大林,景宗森.共育体系中成骨细胞和破骨细胞生物学特性观察.中华骨科杂志(Yang Dehong,Jin Dadi,Chen Jianting,Wu Dalin,Jing Zongsen.The biologicalcharacteristics of osteoblasts and osteoclasts in co-culture sys-tem.Chin J Orthop)2001;21(11):676-80.
35武密山,赵素芝,李恩,白霞.抗骨松丹杞颗粒含药血清对成骨–破骨细胞共培养体系中破骨细胞功能的影响.中国病理生理杂志(Wu Mishan,Zhao Suzhi,Li En,Bai Xia.Effect ofKang Gu Song Dan Qi instant granules contained serum on theactivity of osteoclasts in osteoblasts and osteoclasts co-culturesystem.Chinese Journal of Pathophysiology)2010;26(8):1635-6,1639.
36 Watkins M,Grimston SK,Norris JY,Guillotin B,Shaw A,Be-niash E,et al.Osteoblast Connexin43 Modulates Skeletal Archi-tecture by Regulating Both Arms of Bone Remodeling.Mol BiolCell 2011;22(8):1240-51.
37 Rauch A,Seitz S,Baschant U,Schilling AF,Illing A,Stride B,etal.Glucocorticoids suppress bone formation by attenuating os-teoblast differentiation via the monomeric glucocorticoid recep-tor.Cell Metab 2010;11(6):517-31.
38 Birmingham E,Niebur GL,McHugh PE,Shaw G,Barry FP,McNamara LM.Osteogenic differentiation of mesenchymal stemcells is regulated by osteocyte and osteoblast cells in a simplifiedbone niche.Eur Cell Mater 2012;23:13-27.
39鲁秀敏,陈林,苏楠,李福兵,赵玲.小鼠成骨细胞和破骨细胞共培养模型的建立.中国骨质疏松杂志(Lu Xiumin,ChenLin,Su Nan,Li Fubin,Zhao Ling.Establishment of co-culturemodel of mouse osteoblasts and osteoclasts.Chin J Oseoporos)2008;14(3):159-63.
40 You L,Temiyasathit S,Lee P,Kim CH,Tummala P,Yao W,et al.Osteocytes as mechanosensors in the inhibition of bone resorp-tion due to mechanical loading.Bone 2008;42(1):172-9.
2 Gusm o CV,Belangero WD.How do bone cells sense mechani-cal loading.Rev Bras Ortop 2009;44(4):299-305.
3骞爱荣,胡丽芳,狄升蒙,高翔,孟芮,商澎.骨细胞在力学信号感受过程中的作用.中华航空航天医学杂志(Qian Ai-rong,Hu Lifang,Di Shengmeng,Gao Xiang,Meng Rui,ShangPeng.Roles of osteocytes in mechanosensation.Chin J Aero-space Med)2010;21(2):149-53.
4 Cole RJ,Paul J.Properties of cultured preimplantation mouseand rabbitembryos,and cell strains derived from them.CibaFoundation Symposium-Preimplantation Stages of Pregnancy1965;82-112.
5 James Kirkpatrick C,Fuchs S,Iris Hermanns M,Peters K,UngerRE.Cell culture models of higher complexity in tissue engineer-ing and regenerative medicine.Biomaterials 2007;28(34):5193-8.
6 Wang J,Ren L,Li L,Liu W,Zhou J,Yu W,et al.Microfluidics:A new cosset for neurobiology.Lab Chip 2009;9(5):644-52.
7 Peyrin JM,Deleglise B,Saias L,Vignes M,Gougis P,Magnifico S,etal.Axon diodes for the reconstruction of oriented neuronal net-works in microfluidic chambers.Lab Chip 2011;21(11):3663-73.
8Mow VC,Huiskes R,主编.骨科生物力学暨力学生物学,第3版.汤亭亭,裴国献,李旭,白波,主译.山东:山东科学技术出版社(Mow VC,Huiskes R.Basic Orthopaedic Biomechan-ics and Mechano-Biology,3rd ed.Tang Tingting,Pei Guoxian,Li Xu,Bai Bo,Translate.Shandong:Shangdong Scientific andTechnical Publishers),2009,12-6.
9蒋斯,韩泽民.引导骨组织再生技术在牙槽骨植骨中的应用现状.武警医学院学报(Jiang Si,Han Zeming.Current situationon the clinical application of alveolar augmentation combine withguided bone regeneration.Acta Academiae Medicinae CPAF)2011;20(8):686-8.
10 Riggs BL,Melton LJ 3rd.Evidence for two distinct syndromesof involutional osteoporosis.Am J Med 1983;75(6):899-901.
11 Dempster DW,Lambing CL,Kostenuik PJ,Grauer A.Role ofRANK ligand and denosumab,a targeted RANK ligand inhibi-tor,in bone health and osteoporosis:A review of preclinical andclinical data.Clin Ther 2012;34(3):521-36.
12丁冲,杨宪园,杨鹏飞,商澎.关于机械振动对抗骨量丢失的研究进展.中华物理医学与康复杂志(Ding Chong,Yang Xi-anyuan,Yang Pengfei,Shang Peng.Advance in countermeasureagainst bone loss of vibration.Chin J Phys Med Rehabil)2010;32(10):795-7.
13丁冲,杨鹏飞,商澎.用于预防和治疗骨丢失的机械振动装置.北京生物医学工程(Ding Chong,Yang Pengfei,ShangPeng.A mechanical vibration device for preventing and treatingbone loss.Beijing Biomedical Engineering)2009;28(2):165-9.
14 Bonewald LF.The amazing osteocyte.J Bone Miner Res 2011;26(2):229-38.
15 Pead MJ,Suswillo R,Skerry TM,Vedi S,Lanyon LE.Increased3H-uridine levels in osteocytes following a single short periodof dynamic bone loading in vivo.Calcif Tissue Int 1988;43(2):92-6.
16 Kamioka H,Honjo T,Takano-Yamamoto T.A three-dimensionaldistribution of osteocyte processes revealed by the combinationof confocal laser scanning microscopy and differential interfer-ence contrast microscopy.Bone 2001;28(2):145-9.
17 Kamioka H,Sugawara Y,Honjo T,Yamashiro T,Takano-Yama-moto T.Terminal differentiation of osteoblasts to osteocytes isaccompanied by dramatic changes in the distribution of actin-binding proteins.J Bone Miner Res 2004;19(3):471-8.
18 Cheng B,Zhao S,Luo J,Sprague E,Bonewald LF,Jiang JX.Ex-pression of functional gap junctions and regulation by fluid flowin osteocyte-like MLO-Y4 cells.J Bone Miner Res 2001;16(2):249-59.
19 Takai E,Mauck RL,Hung CT,Guo XE.Osteocyte viability andregulation of osteoblast function in a 3D trabecular bone explantunder dynamic hydrostatic pressure.J Bone Miner Res 2004;19(9):1403-10.
20 Taylor AF,Saunders MM,Shingle DL,Cimbala JM,Zhou Z,Donahue HJ.Mechanically stimulated osteocytes regulate os-teoblastic activity via gap junctions.Am J Physiol Cell Physiol2007;292(2):545-52.
21 Chan ME,Lu XL,Huo B,Baik AD,Chiang V,Guldberg RE,etal.A trabecular bone explant model of osteocyte-osteoblast co-culture for bone mechanobiology.Cell Mol Bioeng 2009;2(3):405-15.
22 Adachi T,Aonuma Y,Taira K,Hojo M,Kamioka H.Asymmetricintercellular communication between bone bells:propagation ofthe calcium sigmaling.Biochem Biophys Res Commun 2009;389(3):495-500.
23 Kurata K,Heino TJ,Higaki H,V n nen HK.Bone marrow celldifferentiation induced by mechanically damaged osteocytes in3D gel-embedded culture.J Bone Miner Res 2006;21(4):616-25.
24 Yellowley CE,Li Z,Zhou Z,Jacobs CR,Donahue HJ.Functionalgap junctions between osteocytic and osteoblastic cells.J BoneMiner Res 2000;15(2):209-17.
25 Vatsa A,Smit TH,Klein-Nulend J.Extracellular NO signallingfrom a mechanically stimulated osteocyte.J Biomech 2007;40Suppl 1:S89-95.
26 Zhao S,Zhang YK,Harris S,Ahuja SS,Bonewald LF.MLO-Y4osteocyte-like cells support osteoclast formation and activation.JBone Miner Res 2002;17(11):2068-79.
27 Yaccoby S,Wezeman MJ,Zangari M,Walker R,Cottler-Fox M,Gaddy D,et al.Inhibitory effects of osteoblasts and increasedbone formation on myeloma in novel culture systems and a my-elomatous mouse model.Haematologica 2006;91(2):192-9.
28 Zhou H,Mak W,Zheng Y,Dunstan CR,Seibel MJ.Osteoblastsdirectly control lineage commitment of mesenchymal progenitorcells through Wnt signaling.J Biol Chem 2008;283(4):1936-45.
29 Wang IE,Shan J,Choi R,Oh S,Kepler CK,Chen FH,et al.Role of osteoblast–fibroblast interactions in the formation of theligament-to-bone interface.J Orthop Res 2007;25(12):1609-20.
30 Boggs ME,Thompson WR,Farach-Carson MC,Duncan RL,Beebe TP.Co-culture of osteocytes and neurons on a unique pat-terned surface.Biointerphases 2011;6(4):200-9.
31 Masuyama R,Stockmans I,Torrekens S,van Looveren R,MaesC,Carmeliet P,et al.Vitamin D receptor in chondrocytes pro-motes osteoclastogenesis and regulates FGF23 production inosteoblasts.J Clin Invest 2006;116(12):3150-9.
32孟芮,罗明志,王亮,高翔,狄升蒙,商澎.三维回转骨细胞条件培养基对成骨细胞功能的调节作用.中国生物化学与分子生物学报(Meng Rui,Luo Mingzhi,Wang Liang,Gao Xi-ang,Di Shengmeng,Shang Peng.Regulating role of conditionedmedium of osteocytes subjected to 3-D rotation on functions ofosteoblasts.Chinese Journal of Biochemistry and Molecular Bi-ology)2010;26(2):95-102.
33孟芮,续惠云,王哲,张维,商澎.模拟失重骨细胞条件培养基对成骨细胞活性的影响.航天医学与医学工程(MengRui,Xu Huiyun,Wang Zhe,Zhang Wei,Shang Peng.Effects ofconditioned meduim of osteocytes subjeced to simulated weight-lessness on activity of osteoblasts.Space Medicine&MedicalEngineering)2009;22(4):247-51.
34杨德洪,金大地,陈建庭,武大林,景宗森.共育体系中成骨细胞和破骨细胞生物学特性观察.中华骨科杂志(Yang Dehong,Jin Dadi,Chen Jianting,Wu Dalin,Jing Zongsen.The biologicalcharacteristics of osteoblasts and osteoclasts in co-culture sys-tem.Chin J Orthop)2001;21(11):676-80.
35武密山,赵素芝,李恩,白霞.抗骨松丹杞颗粒含药血清对成骨–破骨细胞共培养体系中破骨细胞功能的影响.中国病理生理杂志(Wu Mishan,Zhao Suzhi,Li En,Bai Xia.Effect ofKang Gu Song Dan Qi instant granules contained serum on theactivity of osteoclasts in osteoblasts and osteoclasts co-culturesystem.Chinese Journal of Pathophysiology)2010;26(8):1635-6,1639.
36 Watkins M,Grimston SK,Norris JY,Guillotin B,Shaw A,Be-niash E,et al.Osteoblast Connexin43 Modulates Skeletal Archi-tecture by Regulating Both Arms of Bone Remodeling.Mol BiolCell 2011;22(8):1240-51.
37 Rauch A,Seitz S,Baschant U,Schilling AF,Illing A,Stride B,etal.Glucocorticoids suppress bone formation by attenuating os-teoblast differentiation via the monomeric glucocorticoid recep-tor.Cell Metab 2010;11(6):517-31.
38 Birmingham E,Niebur GL,McHugh PE,Shaw G,Barry FP,McNamara LM.Osteogenic differentiation of mesenchymal stemcells is regulated by osteocyte and osteoblast cells in a simplifiedbone niche.Eur Cell Mater 2012;23:13-27.
39鲁秀敏,陈林,苏楠,李福兵,赵玲.小鼠成骨细胞和破骨细胞共培养模型的建立.中国骨质疏松杂志(Lu Xiumin,ChenLin,Su Nan,Li Fubin,Zhao Ling.Establishment of co-culturemodel of mouse osteoblasts and osteoclasts.Chin J Oseoporos)2008;14(3):159-63.
40 You L,Temiyasathit S,Lee P,Kim CH,Tummala P,Yao W,et al.Osteocytes as mechanosensors in the inhibition of bone resorp-tion due to mechanical loading.Bone 2008;42(1):172-9.