三甲基吡嗪及四甲基吡嗪对急性酒精肝损伤小鼠的保护作用
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摘要
目的探讨三甲基吡嗪(Pyr)、四甲基吡嗪(TMP)及其联合作用对急性酒精肝损伤小鼠的保护作用。方法60只ICR小鼠随机分为5组,分别为对照组、模型组、Pyr组、TMP组及Pyr+TMP组。对照组自由喂养,模型组每日给予0.9%的生理盐水灌胃,其他各组分别给予100 mg/(kg·d)bw的Pyr、TMP及Pyr+TMP灌胃。30d后,造急性酒精肝损伤模型,常规方法制备小鼠血清及肝匀浆,测定血清中总胆红素(TB)、谷草转氨酶(sGOT)、谷丙转氨酶(sGPT)、碱性磷酸酶(sALP)、血糖、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、乙醇脱氢酶(ADH)及肝匀浆中丙二醛(MDA)、谷胱甘肽(GSH)含量。结果(1)提高ADH活性:Pyr+TMP组>TMP组>Pyr组;(2)降低因急性酒精肝损伤引起的sGOT和sGPT:Pyr组>Pyr+TMP组>TMP组;(3)降低血糖和血脂,抗脂质过氧化活性:Pyr+TMP组>Pyr组>TMP组。结论 100 mg/(kg·d)bw的Pyr、TMP及两者联合使用对于急性酒精肝损伤小鼠均具有很好的保护作用,且Pyr和TMP联合使用效果最佳。
Objective To study the protective effects of 2,3,5-trimethylpyrazine(Pyr) and 2,3,5,6-tetra methylpyrazine(TMP) on acute alcoholic liver injury in mice. Methods Sixty healthy mice rats were randomly divided into five groups:control group, model group, Pyr group, TMP group and Pyr+ TMP group. The mice were given saline or 100 mg/(kg·d) bw drug for 30 d, 12 ml/kg volume fraction of 50% ethanol were given to all groups except the control. The level of total bilirubin(TB), glutamate-oxalacetate transaminease(sGOT), glutamate pyruvate transaminase(sGPT), alkaline phosphatase(sALP), blood glucose, total cholesterol(TC), total triglycerides(TG), high-density lipoprotein(HDL-C), low-density lipoprotein(LDL-C), alcohol dehydrogenase(ADH) in serum and the content of glutathione(GSH), malondialdehyde(MDA)in liver were detected. Results(1)Improvement of the ADH activity: Pyr+TMP > TMP >Pyr;(2)Reduction of sGOT and sGPT: Pyr >Pyr+TMP > TMP;(3)Reduction of the level of glucose and serum lipid, and anti-lipid peroxidation activity:Pyr+TMP > Pyr > TMP. Conclusion Pyr and TMP(100 mg/kg bw·d) had an effective protection against acute alcoholic liver injury in mice and Pyr+TMP was the best.
Objective To study the protective effects of 2,3,5-trimethylpyrazine(Pyr) and 2,3,5,6-tetra methylpyrazine(TMP) on acute alcoholic liver injury in mice. Methods Sixty healthy mice rats were randomly divided into five groups:control group, model group, Pyr group, TMP group and Pyr+ TMP group. The mice were given saline or 100 mg/(kg·d) bw drug for 30 d, 12 ml/kg volume fraction of 50% ethanol were given to all groups except the control. The level of total bilirubin(TB), glutamate-oxalacetate transaminease(sGOT), glutamate pyruvate transaminase(sGPT), alkaline phosphatase(sALP), blood glucose, total cholesterol(TC), total triglycerides(TG), high-density lipoprotein(HDL-C), low-density lipoprotein(LDL-C), alcohol dehydrogenase(ADH) in serum and the content of glutathione(GSH), malondialdehyde(MDA)in liver were detected. Results(1)Improvement of the ADH activity: Pyr+TMP > TMP >Pyr;(2)Reduction of sGOT and sGPT: Pyr >Pyr+TMP > TMP;(3)Reduction of the level of glucose and serum lipid, and anti-lipid peroxidation activity:Pyr+TMP > Pyr > TMP. Conclusion Pyr and TMP(100 mg/kg bw·d) had an effective protection against acute alcoholic liver injury in mice and Pyr+TMP was the best.
引文
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[2]吴婷婷,孙惜时,冉昇,等.芝麻香型白酒对大鼠血脂血糖和肝功能的影响[J].酿酒科技,2013:46-49.
[3]庄名扬.再论美拉德反应产物与中国白酒的香和味[J].酿酒科技,2005:34-38.
[4]吴建峰.中国白酒中健康功能性成分四甲基吡嗪的研究[J].酿酒科技,2007:117-120.
[5]杨涛,李国友,吴林蔚,等.中国白酒健康因子的研究及其产生菌选育和在生产中的应用(I).中国白酒健康因子的研究[J].酿酒科技,2010:65-69.
[6]Shan Au AL,Kwan YW,Kwok CC,et al.Mechanisms responsible for the in vitro relaxation of ligustrazine on porcine left anterior descending coronary artery[J].Eur J Pharmacol,2003,468:199-207.
[7]Yang QH,Liang Y,Xu Q,et al.Protective effect of tetramethylpyrazine isolated from Ligusticum chuanxiong on nephropathy in rats with streptozotocininduced diabetes[J].Phytomedicine,2011,18:1148-1152.
[8]彭易清,林婉媚,聂伟明,等.长期危险饮酒与脂代谢糖耐量变化及动脉硬化的关系[J].检验医学与临床,2008,5:197-199.
[9]谢屹.长期饮酒与血压血脂和血糖的关系[J].华西医学,2008,23:1513-1514.
[10]Sarem IA,Arora R.The cardiovascular implications of alcohol and red wine[J].Am J Therap,2008,15:265-277.
[11]郑曼,蔡九英.不同剂量白酒对大鼠血管内皮功能血脂和血糖的影响[J].山东医药,2011,51:19-21.
[12]Zheng H,Chen XL,Han ZX,et al.Ligustrazine attenuates acute lung injury after burn trauma[J].Burns,2005,31:453-458.
[13]王宝恩,张定凤.现代肝脏病学[M].北京:科学出版社,2003:186-187,195.
[14]Chen HP,He M,Huang QR,et al.Delayed protection of tetramethylpyrazine on neonatal rat cardiomyocytes subjected to anoxia-reoxygenation injury[J].Basic Clin Pharmacol,2007,100:366-371.
[15]褚燕君,许景伟.川芎嗪对酒精性肝炎患者血清SOD LPO T NF-αIL-8 PC-III与HA的影响[J].中国临床综合,2003,19:597-598.