低分子质量和寡聚透明质酸制备、活性与应用研究进展
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摘要
透明质酸是一种高分子糖胺聚糖,不同相对分子质量(M_r)的透明质酸具有不同的生物活性。文章主要对近年来低M_r和寡聚透明质酸的制备方法、生物活性及其应用的研究进展进行了综述,为将来进一步研究利用低M_r和寡聚透明质酸提供了理论依据和新思路。检索阅读近几年国内外发表的与低M_r和寡聚透明质酸相关的研究文献,对原始研究论文中的数据和主要观点进行了分析整理并归纳总结。通过分析文献发现,近年来对低M_r及寡聚透明质酸的制备方法和它们在抗氧化、促进骨和血管生成、促进伤口愈合、免疫调节等生物活性研究较多,并且在临床医学、药品、化妆品、保健食品等领域的应用均有研究。LMW-HA和oligo-HA表现出的与普通高M_r HA截然不同的生物活性,随着研究的不断深入,其在临床医学、药品、化妆品、保健食品等领域将有更广阔的应用前景。
Hyaluronic acid is one kind of glycosaminoglycans of high molecular weight,and the biological activities of it associate with its molecular weight. To provide theoretical basis and new ideas for further research on the use of low molecular weight and oligomeric hyaluronic acid( LMW-HA and oligo-HA) in the future,this paper has reviewed the progress on the preparation methods,bioactivities and applications of them in recent years. The research literatures related to LMW-HA and oligo-HA published in recent years were searched,analyzed and summarized. By reviewing the research literatures it can be found that progresses have been made on the preparation methods,bioactivities and applications of LMW-HA and oligo-HA in recent years,especially in the studies of the activities of antioxidation,osteogenesis and angiogenesis acceleration,wound healing promotion,immune regulation and the applications in the fields of medicine,cosmetics and health foods. The biological activities of LMW-HA and oligo-HA are distinctly different from those of natural high M_rHA. With the deepening of research,they will have wide use in the fields of clinical medicine,pharmaceuticals,cosmetics,health food and so on.
Hyaluronic acid is one kind of glycosaminoglycans of high molecular weight,and the biological activities of it associate with its molecular weight. To provide theoretical basis and new ideas for further research on the use of low molecular weight and oligomeric hyaluronic acid( LMW-HA and oligo-HA) in the future,this paper has reviewed the progress on the preparation methods,bioactivities and applications of them in recent years. The research literatures related to LMW-HA and oligo-HA published in recent years were searched,analyzed and summarized. By reviewing the research literatures it can be found that progresses have been made on the preparation methods,bioactivities and applications of LMW-HA and oligo-HA in recent years,especially in the studies of the activities of antioxidation,osteogenesis and angiogenesis acceleration,wound healing promotion,immune regulation and the applications in the fields of medicine,cosmetics and health foods. The biological activities of LMW-HA and oligo-HA are distinctly different from those of natural high M_rHA. With the deepening of research,they will have wide use in the fields of clinical medicine,pharmaceuticals,cosmetics,health food and so on.
引文
[1]郭学平,凌沛学,张天民.低分子质量及寡聚玻璃酸[J].中国生化药物杂志,2003,24(3):148-150.
[2]黄岳山,潘艺茗,薛静.不同相对分子质量透明质酸功能及应用的研究[J].透析与人工器官,2011,21(2):22-25.
[3]蒋延超,蒋世云,傅凤鸣,等.透明质酸生物合成途径及基因工程研究进展[J].中国生物工程杂志,2015,35(1):104-110.
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[5]Ke C,Sun L,Qiao D,et al.Antioxidant acitivity of low molecular weight hyaluronic acid[J].Food Chem Toxicol,2011,49(10):2670-2675.
[6]高瑞昌,陈辉,李来好,等.低分子质量罗非鱼眼透明质酸的制备及其抗氧化性研究[J].食品工业科技,2015,36(3):60-64.
[7]Euppayo T,Siengdee P,Buddhachat K,et al.Effects of low molecular weight hyaluronan combined with carprofen on canine osteoarthritis articular chondrocytes and cartilage explants in vitro[J].In Vitro Cell Dev Biol Anim,2015,51(8):857-865.
[8]Huang TL,Hsu HC,Yang KC,et al.Effect of different molecular weight hyaluronans on osteoarthritis-related protein production in fibroblast-like synoviocytes from patients with tibia plateau fracture[J].J Trauma,2010,68(1):146-152.
[9]Slevin M,Krupinski J,Gaffney J,et al.Hyaluronan-mediated angiogenesis in vascular disease:uncovering RHAMM and CD44receptor signaling pathways[J].Matrix Biol,2007,26(1):58-68.
[10]杨翠霞,刘鷖雯,何怡青,等.低分子质量透明质酸寡糖片段介导内皮细胞增殖的信号通路[J].中国生物化学与分子生物学报,2008,24(3):268-275.
[11]赵京玉,柴家科.外源性透明质酸对创面愈合影响的研究进展[J].中华损伤与修复杂志(电子版),2011,06(1):65-67.
[12]Kavasi RM,Berdiaki A,Spyridaki I,et al.HA metabolism in skin homeostasis and inflammatory disease[J].Food Chem Toxicol,2017,101(3):128-138.
[13]D'Agostino A,Stellavato A,Busico T,et al.In vitro analysis of the effects on wound healing of high-and low-molecular weight chains of hyaluronan and their hybrid H-HA/L-HA complexes[J].Bmc Cell Biol,2015,16(1):19-44.
[14]Litwiniuk M,Krejner A,Speyrer MS,et al.Hyaluronic acid in inflammation and tissue regeneration[J].Wounds,2016,28(3):78-88.
[15]Suga H,Sugaya M,Fujita H,et al.TLR4,rather than TLR2,regulates wound healing through TGF-b and CCL5 expression[J].JDermatol Sci,2014,73(2):117-124.
[16]Bourguignon LYW,Wong G,Earle C A,et al.Interaction of low molecular weight hyaluronan with CD44 and toll-like receptors promotes the actin filament-associated protein 110-actin binding and My D88-NFκB signaling leading to proinflammatory cytokine/chemokine production and breast tumor invasion[J].Cytoskeleton,2011,68(12):671-693.
[17]柯春林,李作美,曾晓雄.低分子质量透明质酸对小鼠巨噬细胞和T细胞的免疫调节作用[J].食品工业科技,2016,37(15):349-353.
[18]Fuchs K,Hippe A,Schmaus A,et al.Opposing effects of high-and low-molecular weight hyaluronan on CXCL12-induced CXCR4signaling depend on CD44[J].Cell Death Dis,2013,4(10):819-830.
[19]Thompson CB,Shepard HM,O'Connor PM,et al.Enzymatic depletion of tumor hyaluronan induces antitumor responses in preclinical animal models[J].Mol Cancer Ther,2010,9(11):3052-3064.
[20]Yang C,Liu Y,He Y,et al.The use of HA oligosaccharide-loaded nanoparticles to breach the endogenous hyaluronan glycocalyx for breast cancer therapy[J].Biomaterials,2013,34(28):6829-6838.
[21]刘婷先,冯立霞,刘凤喜,等.透明质酸修饰阳离子脂质体的制备及初步评价[J].药物生物技术,2013,20(05):400-404.
[22]李娜,周伟芳,刘慧敏,等.疫苗佐剂的研究现状和发展趋势[J].中国预防兽医学报,2013,35(1):81-86.
[23]施建东,胡凝珠,赵蕊蕊,等.氢氧化锌与低分子质量透明质酸复合佐剂对甲肝乙肝联合抗原的体液免疫增强作用[J].中国生物制品学杂志,2012,25(1):29-31.
[24]Ran W,Wang X,Hu Y,et al.Crosslinking sodium hyaluronate gel with different ratio of molecular weight for subcutaneous injection:animal experimental study and clinical trials subcutaneous injection[J].Chines J Plast Surg,2015,31(3):198-201.
[25]Doleckova I,Moravcova M,Maresova M,et al.24th conference of the International Federation of Society of Cosmetic Chemists[C].Korea:IFSCC Conference,2017.
[26]Chinatsu K,Takushi Y,Hideto Y,et al.Ingested hyaluronan moisturizes dry skin[J].Nutr J,2014,13(1):1-9.
[27]冯宁,石艳丽,郭风仙,等.口服透明质酸对皮肤水分的改善作用及体内抗氧化作用研究[J].食品与药品,2016,18(6):386-390.
[28]蒋秋燕,凌沛学,黄思玲,等.口服透明质酸在大鼠体内吸收机制的研究[J].中国药学杂志,2005,40(23):1811-1813.
[2]黄岳山,潘艺茗,薛静.不同相对分子质量透明质酸功能及应用的研究[J].透析与人工器官,2011,21(2):22-25.
[3]蒋延超,蒋世云,傅凤鸣,等.透明质酸生物合成途径及基因工程研究进展[J].中国生物工程杂志,2015,35(1):104-110.
[4]刘浩,孙夏青.低分子质量透明质酸的制备方法及工程菌[P].中国,CN103993022A,2014.
[5]Ke C,Sun L,Qiao D,et al.Antioxidant acitivity of low molecular weight hyaluronic acid[J].Food Chem Toxicol,2011,49(10):2670-2675.
[6]高瑞昌,陈辉,李来好,等.低分子质量罗非鱼眼透明质酸的制备及其抗氧化性研究[J].食品工业科技,2015,36(3):60-64.
[7]Euppayo T,Siengdee P,Buddhachat K,et al.Effects of low molecular weight hyaluronan combined with carprofen on canine osteoarthritis articular chondrocytes and cartilage explants in vitro[J].In Vitro Cell Dev Biol Anim,2015,51(8):857-865.
[8]Huang TL,Hsu HC,Yang KC,et al.Effect of different molecular weight hyaluronans on osteoarthritis-related protein production in fibroblast-like synoviocytes from patients with tibia plateau fracture[J].J Trauma,2010,68(1):146-152.
[9]Slevin M,Krupinski J,Gaffney J,et al.Hyaluronan-mediated angiogenesis in vascular disease:uncovering RHAMM and CD44receptor signaling pathways[J].Matrix Biol,2007,26(1):58-68.
[10]杨翠霞,刘鷖雯,何怡青,等.低分子质量透明质酸寡糖片段介导内皮细胞增殖的信号通路[J].中国生物化学与分子生物学报,2008,24(3):268-275.
[11]赵京玉,柴家科.外源性透明质酸对创面愈合影响的研究进展[J].中华损伤与修复杂志(电子版),2011,06(1):65-67.
[12]Kavasi RM,Berdiaki A,Spyridaki I,et al.HA metabolism in skin homeostasis and inflammatory disease[J].Food Chem Toxicol,2017,101(3):128-138.
[13]D'Agostino A,Stellavato A,Busico T,et al.In vitro analysis of the effects on wound healing of high-and low-molecular weight chains of hyaluronan and their hybrid H-HA/L-HA complexes[J].Bmc Cell Biol,2015,16(1):19-44.
[14]Litwiniuk M,Krejner A,Speyrer MS,et al.Hyaluronic acid in inflammation and tissue regeneration[J].Wounds,2016,28(3):78-88.
[15]Suga H,Sugaya M,Fujita H,et al.TLR4,rather than TLR2,regulates wound healing through TGF-b and CCL5 expression[J].JDermatol Sci,2014,73(2):117-124.
[16]Bourguignon LYW,Wong G,Earle C A,et al.Interaction of low molecular weight hyaluronan with CD44 and toll-like receptors promotes the actin filament-associated protein 110-actin binding and My D88-NFκB signaling leading to proinflammatory cytokine/chemokine production and breast tumor invasion[J].Cytoskeleton,2011,68(12):671-693.
[17]柯春林,李作美,曾晓雄.低分子质量透明质酸对小鼠巨噬细胞和T细胞的免疫调节作用[J].食品工业科技,2016,37(15):349-353.
[18]Fuchs K,Hippe A,Schmaus A,et al.Opposing effects of high-and low-molecular weight hyaluronan on CXCL12-induced CXCR4signaling depend on CD44[J].Cell Death Dis,2013,4(10):819-830.
[19]Thompson CB,Shepard HM,O'Connor PM,et al.Enzymatic depletion of tumor hyaluronan induces antitumor responses in preclinical animal models[J].Mol Cancer Ther,2010,9(11):3052-3064.
[20]Yang C,Liu Y,He Y,et al.The use of HA oligosaccharide-loaded nanoparticles to breach the endogenous hyaluronan glycocalyx for breast cancer therapy[J].Biomaterials,2013,34(28):6829-6838.
[21]刘婷先,冯立霞,刘凤喜,等.透明质酸修饰阳离子脂质体的制备及初步评价[J].药物生物技术,2013,20(05):400-404.
[22]李娜,周伟芳,刘慧敏,等.疫苗佐剂的研究现状和发展趋势[J].中国预防兽医学报,2013,35(1):81-86.
[23]施建东,胡凝珠,赵蕊蕊,等.氢氧化锌与低分子质量透明质酸复合佐剂对甲肝乙肝联合抗原的体液免疫增强作用[J].中国生物制品学杂志,2012,25(1):29-31.
[24]Ran W,Wang X,Hu Y,et al.Crosslinking sodium hyaluronate gel with different ratio of molecular weight for subcutaneous injection:animal experimental study and clinical trials subcutaneous injection[J].Chines J Plast Surg,2015,31(3):198-201.
[25]Doleckova I,Moravcova M,Maresova M,et al.24th conference of the International Federation of Society of Cosmetic Chemists[C].Korea:IFSCC Conference,2017.
[26]Chinatsu K,Takushi Y,Hideto Y,et al.Ingested hyaluronan moisturizes dry skin[J].Nutr J,2014,13(1):1-9.
[27]冯宁,石艳丽,郭风仙,等.口服透明质酸对皮肤水分的改善作用及体内抗氧化作用研究[J].食品与药品,2016,18(6):386-390.
[28]蒋秋燕,凌沛学,黄思玲,等.口服透明质酸在大鼠体内吸收机制的研究[J].中国药学杂志,2005,40(23):1811-1813.