肠愈宁对溃疡性结肠炎大鼠炎性因子及钙卫蛋白释放的影响
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摘要
目的:研究肠愈宁颗粒对二硝基氯苯乙酸复合法诱导大鼠溃疡性结肠炎的作用,并初步探讨其作用机制。方法:将雄性SD大鼠分成6组:正常组、模型组、柳氮磺胺吡啶组(SASP)和肠愈宁颗粒低、中、高剂量组。以二硝基氯苯乙酸复合法诱导大鼠溃疡性结肠炎模型。评估疾病活动指数(DAI)、结肠黏膜损伤指数(CMDI)和病理组织学。ELISA方法测定血清IL-β、TNF-α、钙卫蛋白(Calproteein,CP),Western blot方法检测结肠黏膜CP的表达水平。结果:DAI结果提示造模成功,与正常组比较,模型组血清中IL-β、TNF-α、CP均明显升高,而CMDI指数、结肠黏膜CP均明显升高。肠愈宁颗粒各组DAI、CMDI、结肠组织中黏膜CP均降低,血清中IL-β、TNF-α、CP水平也明显降低。结论:肠愈宁颗粒能够有效缓解二硝基氯苯乙酸复合法诱导的大鼠溃疡性结肠炎,其作用机制可能与下调IL-β、TNF-α表达,减少CP水平有关。
Objective: To study the effects of Changyuning Granule( CYN) on ulcerative colitis induced by dinitrochlorobenzene/ethanoic acid in rats,and to explore its mechanism. Methods: Male SD rats were divided into 6 groups: the normal group,the model group,the low,medium and high dose group of CYN and the sulfonylamine group( SASP). The ulcerative colitis model was induced by dinitrochlorobenzene/ethanoic acid. Disease activity index( DAI),colonic mucosa damage index( CMDI) and pathological histology were assessed. Serum IL-β,TNF-α,and calcium protein( Calproteein,CP) were detected by ELISA. The expression of CP in colonic mucosa was detected by Western blot. Results: DAI results suggested that the model was successful. Compared with the normal group,IL-β,TNF-α,and CP in the serum of the model group were significantly increased,and the index of CMDI,colonic mucosa CP were significantly increased. The DAI,CMDI,and mucosal CP in the colon tissue of each group of Changyuning Granules were decreased,and the levels of IL-,TNF-,and CP in serum were also significantly decreased. Conclusion: CYN Granule can effectively relieve the ulcerative colitis induced by dinitrochlorobenzene/ethanoic acid in rats. The mechanism may be related to down-regulation of IL-β and TNF-α expression and reduction of CP level.
Objective: To study the effects of Changyuning Granule( CYN) on ulcerative colitis induced by dinitrochlorobenzene/ethanoic acid in rats,and to explore its mechanism. Methods: Male SD rats were divided into 6 groups: the normal group,the model group,the low,medium and high dose group of CYN and the sulfonylamine group( SASP). The ulcerative colitis model was induced by dinitrochlorobenzene/ethanoic acid. Disease activity index( DAI),colonic mucosa damage index( CMDI) and pathological histology were assessed. Serum IL-β,TNF-α,and calcium protein( Calproteein,CP) were detected by ELISA. The expression of CP in colonic mucosa was detected by Western blot. Results: DAI results suggested that the model was successful. Compared with the normal group,IL-β,TNF-α,and CP in the serum of the model group were significantly increased,and the index of CMDI,colonic mucosa CP were significantly increased. The DAI,CMDI,and mucosal CP in the colon tissue of each group of Changyuning Granules were decreased,and the levels of IL-,TNF-,and CP in serum were also significantly decreased. Conclusion: CYN Granule can effectively relieve the ulcerative colitis induced by dinitrochlorobenzene/ethanoic acid in rats. The mechanism may be related to down-regulation of IL-β and TNF-α expression and reduction of CP level.
引文
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[12]王少东,汪芳裕,万海军,等,钙卫蛋白监测在炎症性肠病患者中的意义[J].胃肠病学和肝病学杂志,2010,19(11):1043-1044.
[13]Lv H,Hao SY,Yan NB. Faecal calprotectin meaning differential diag-nosis among inflammatory bowel disease,irritable bowel syndrome colo-rectal cancer[J]. Int J Lab Med,2012,33(21):2651-2652.
[2]路晓红,杨恩来,赵霞,等.溃疡性结肠炎免疫机制的研究进展[J].山西中医学院学报,2011,12(1):66-68.
[3]朱毅,赵锦,陈国彪,等.海南砂仁挥发油对2,4-二硝基氯苯与乙酸诱发的大鼠溃疡性结肠炎的治疗作用[J].中国药理学与毒理学杂志,2009,23(5):388-394.
[4]王坤,宣秀敏,王莲,等.溃疡性结肠炎大鼠外周血CD4+CD25+Treg的变化及相关性研究[J].安徽医科大学学报,2012,47(3):354-356.
[5]Christine C,Michel C,Lecannu G. The prebiotic characteristics of fructooligo saccharides are necessary for reduction of TNBS induced colitis in rats[J]. J Nutr,2003,133(1):21-27.
[6]郑家驹.炎症性肠病的维持缓解治疗[J].中华消化杂志,2007,27(12):852-854.
[7]谢晶日,林静.肠愈宁颗粒治疗溃疡性结肠炎临床疗效观察[J].中国中西医结合消化杂志,2010,18(3):161.
[8]赵曼,高峰.溃疡性结肠炎发病机制研究进展[J].现代医学生物进展,2011,11(16):170.
[9]Nguyen GC,TuskeyA,DassopoulosT,etal. Rising hospitalization rates for inflammatory bowel disease in the United States between 1998 and2004[J]. Inflammatory bowel diseases,2007,13:1529-1535.
[10]Brun JG,Haga HJ,Boe E,et al. Calprotectin in patient s with rheumat oid art hritis:relation toclinical and laboratory variables of disease activity[J]. J Rheumatol,1992,19(6):859-862.
[11]王伟宁,张熙纯,刘丽.活动期溃疡性结肠炎发病机制的免疫学讨论[J].中国现代医学杂志,2003,13(16):74.
[12]王少东,汪芳裕,万海军,等,钙卫蛋白监测在炎症性肠病患者中的意义[J].胃肠病学和肝病学杂志,2010,19(11):1043-1044.
[13]Lv H,Hao SY,Yan NB. Faecal calprotectin meaning differential diag-nosis among inflammatory bowel disease,irritable bowel syndrome colo-rectal cancer[J]. Int J Lab Med,2012,33(21):2651-2652.