摘要
本研究对12个香稻材料Badh2基因第7外显子序列进行PCR扩增和序列分析,利用籽粒咀嚼法、KOH浸泡法对香稻材料进行香味鉴定,以分析香稻材料Badh2基因第7外显子的序列突变及其与香味性状的对应关系。结果表明,香糯、绿金香、美香占2号、华香、越香选1号、红芋香、美香选1号、巴香选1号、泰香选1号、泰香选2号10个材料Badh2基因的第7外显子均发生了8 bp的缺失(aaaa--t——ggc)和1 bp的突变(g→t),与GenBank的EU155083序列的突变一致,在中国水稻材料中首次发现该突变。在发生突变的10个香稻材料中,7个材料被鉴定为香稻品种,其中美香占2号和越香选1号的糙米有甜味;华香和美香选1号被鉴定为杂合基因型,红芋香被鉴定为非香稻。香稻材料Badh2基因第7外显子的序列突变与其香味性状不能一一对应。研究结果为水稻香味性状遗传机制研究和香稻品种的推广提供了参考。
In this study, we performed the amplificaion and the sequence analysis of exon7 of Badh2 gene of 12 fragrant rice materials, and identified the aroma of fragrant rice materials by the method of seed chewing and KOH soaking, to analysed the sequence mutation of exon 7 in the Badh2 gene of rice material and its corresponding relation with the flavor character. The results showed that an 8 bp deletion(aaaa--t---ggc) and a mutation of SNP(g →t) in exon 7 of Badh2 gene were found in 10 materials, including Xiangnuo, Lüjinxiang, Meixiangzhan 2,Huaxiang, Yuexiangxuan 1, Hongyuxiang, Meixiangxuan 1, Baxiangxuan 1, Taixiangxuan 1, Taixiangxuan 2.This mutaion was consistent with the mutation of EU155083 sequence in GenBank and was reported for the first time in Chinese rice materials. In these 10 fragrant rice materials with mutation, 7 rice materials were identified as fragrant rice where the brown rice of Meixiangzhan 2 and Yuexiangyuan 1 had sweet taste, Huaxiang and Meixiangxuan 1 were identified as the heterozygote genotype, and Hongyuxiang was identified as the non-fragrant rice. So the sequence mutation in exon 7 of Badh2 gene in fragrant rice materials didn't correspond to aroma traits one by one. Our results could provide a reference for the research on the genetic mechanism of rice aroma character and the promotion of the rice variety.
引文
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